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Proceedings Paper

Flash contact x-ray microscopy of biological specimen in water
Author(s): Toshihisa Tomie; Hazime Shimizu; Toshikazu Majima; Toshihiko Kanayama; Mitsuo Yamada; Eisuke Miura
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Paper Abstract

From the shot noise consideration in x-ray microscopy it is shown that, for 50 nm resolution, a few tens mJ/cm2 energy of 3 nm x ray is required on the specimen, and required x ray energy density increases with the fourth power of the resolving power. X-ray images of biological specimens in water taken by flash contact microscopy with a laser-plasma source are presented to discuss the prospects of x-ray microscopy for biological applications. Forty nanometer diameter biological components are observed in an x-ray image. The attempt of observing thick specimens by short wavelength x rays produced by a 10 ps duration laser pulse is also reported. Technical issues for further improving contact microscopy, including the feasibility of stereo-microscopy, are discussed.

Paper Details

Date Published: 13 January 1993
PDF: 11 pages
Proc. SPIE 1741, Soft X-Ray Microscopy, (13 January 1993); doi: 10.1117/12.138725
Show Author Affiliations
Toshihisa Tomie, Electrotechnical Lab. (Japan)
Hazime Shimizu, Electrotechnical Lab. (Japan)
Toshikazu Majima, Electrotechnical Lab. (Japan)
Toshihiko Kanayama, Electrotechnical Lab. (Japan)
Mitsuo Yamada, Electrotechnical Lab. (Japan)
Eisuke Miura, Electrotechnical Lab. (Japan)

Published in SPIE Proceedings Vol. 1741:
Soft X-Ray Microscopy
Chris J. Jacobsen; James E. Trebes, Editor(s)

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