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Proceedings Paper

Toxicity, pharmacokinetics, and photodynamic properties of chlorin e6
Author(s): Gennady Kostenich; Ivan N. Zhuravkin; G. P. Gurinovich; Edvard A. Zhavrid M.D.
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Paper Abstract

Toxicity, pharmacokinetics, and the tumor damage effect of chlorin e6 after light irradiation were studied. The results show that chlorin e6 LD50 value in C57Bl mice was 189 +/- 10 mg/kg, in non-inbred white rats it was 99 +/- 14 mg/kg 14 days after the agent iv injection. The concentration of chlorin e6 in blood, liver, kidney, spleen, and tumors (sarcoma M-1 and sarcoma 45) of the rats was determined by the fluorescence method 3, 6, 12, 18, 24, 48, and 72 hours after the agent iv injection at the dose of 10 mg/kg. For this purpose chlorin e6 was extracted from tissues by detergent triton X-100. The depth of necrosis spreading in tumor tissue was evaluated after chlorin e6 injection at the doses of 1 - 10 mg/kg and subsequent irradiation by a krypton laser with light energy density of 90 J/cm2, using the method of vital staining with Evans blue. It was found that depending on the agent dose and time interval between chlorin e6 injection and photoradiation, the depth of tumor necrosis varied from 4.0 to 16.6 mm in sarcoma M-1 and from 5.0 to 15.0 in sarcoma 45.

Paper Details

Date Published: 5 March 1993
PDF: 16 pages
Proc. SPIE 1616, International Conference on Photodynamic Therapy and Laser Medicine, (5 March 1993); doi: 10.1117/12.136990
Show Author Affiliations
Gennady Kostenich, Institute of Oncology and Medical Radiology (Belarus)
Ivan N. Zhuravkin, Institute of Oncology and Medical Radiology (Belarus)
G. P. Gurinovich, Institute of Physics (Belarus)
Edvard A. Zhavrid M.D., Institute of Oncology and Medical Radiology (Belarus)

Published in SPIE Proceedings Vol. 1616:
International Conference on Photodynamic Therapy and Laser Medicine
Junheng Li M.D., Editor(s)

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