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Proceedings Paper

Effect of photodynamic therapy on mouse platelets
Author(s): Chuannong Zhou; Shunji Chi; Jinsheng Deng; Hua Zhang; Junlin Liang; Xian-wen Ha
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Paper Abstract

Normal mice received hematoporphyrin derivative (10 mg/kg iv) immediately, 24 or 48 hrs prior to red light irradiation. The blood was collected and the platelet-rich plasma was irradiated by red light (100 J/cm2). The platelets were fixed immediately, 8 or 16 hrs after irradiation, and processed for EM examination. In comparison with those of control mice, the platelets of all experimental mice showed structural changes: 16 hrs after irradiation all platelets were necrotized; 8 hrs after irradiation almost one fourth of the platelets were necrotized and the remaining were considerably damaged; immediately after irradiation a small number of platelets became necrotic and most other platelets were swollen and deformed, often with many cytoplasmic projections and considerable dilatation of the canalicular membrane system. Our findings provided a clear evidence that platelets are highly sensitive to PDT action and can be directly and rapidly injured by PDT even in the absence of vascular endothelial cells. Our results give firm support to the hypothesis that both endothelial cells and platelets may play an important role in the initiation of early vascular damage and microcirculatory alterations induced by PDT in vivo.

Paper Details

Date Published: 5 March 1993
PDF: 6 pages
Proc. SPIE 1616, International Conference on Photodynamic Therapy and Laser Medicine, (5 March 1993); doi: 10.1117/12.136976
Show Author Affiliations
Chuannong Zhou, Cancer Institute (China)
Shunji Chi, Cancer Institute (China)
Jinsheng Deng, Cancer Institute (China)
Hua Zhang, Cancer Institute (China)
Junlin Liang, Cancer Institute (China)
Xian-wen Ha, Cancer Institute (China)

Published in SPIE Proceedings Vol. 1616:
International Conference on Photodynamic Therapy and Laser Medicine
Junheng Li M.D., Editor(s)

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