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Proceedings Paper

Study of ABO blood types by combining membrane electrophoresis with surface-enhanced Raman spectroscopy
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Paper Abstract

The molecular characterization of ABO blood types, which is clinically significant in blood transfusion, has clinical and anthropological importance. Polymerase chain reaction sequence-based typing (PCR-SBT) is one of the most commonly used methods for the analysis of genetic bases of ABO blood types. However, such methods as PCR-SBT are time-consuming and are high in demand of equipments and manipulative skill. Here we showed that membrane electrophoresis based SERS method employed for studying the molecular bases of ABO blood types can provide rapidand easy-operation with high sensitivity and specificity. The plasma proteins were firstly purified by membrane electrophoresis and then mixed with silver nanoparticles to perform SERS detection. We use this method to classify different blood types, including blood type A (n=13), blood type B (n=9) and blood type O (n=10). Combination of principal component analysis (PCA) and liner discriminant analysis (LDA) was then performed on the SERS spectra of purified albumin, showing good classification results among different blood types. Our experimental outcomes represent a critical step towards the rapid, convenient and accurate identification of ABO blood types.

Paper Details

Date Published: 11 December 2012
PDF: 6 pages
Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 855323 (11 December 2012); doi: 10.1117/12.1000238
Show Author Affiliations
Jing Wang, Fujian Normal Univ. (China)
Juqiang Lin, Fujian Normal Univ. (China)
Zufang Huang, Fujian Normal Univ. (China)
Liqing Sun, First Hospital of Fuzhou (China)
Yonghong Shao, Shenzhen Univ. (China)
Peng Lu, Fujian Normal Univ. (China)
Wei Shi, Fujian Normal Univ. (China)
Jinyong Lin, Fujian Normal Univ. (China)
Rong Chen, Fujian Normal Univ. (China)

Published in SPIE Proceedings Vol. 8553:
Optics in Health Care and Biomedical Optics V
Qingming Luo; Ying Gu; Xingde D. Li, Editor(s)

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