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Proceedings Paper

Second harmonic generation imaging of skin wound healing and scarring in a rabbit ear model
Author(s): Yiyan Tang; Xiaoqin Zhu; Shuyuan Xiong; Jianxin Chen
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Paper Abstract

Skin wound healing and scarring in rabbit ears was examined by second harmonic generation (SHG) microscopy. Rabbit ear wound model was created by punching from the ventral surface with removal of epidermis, dermis and perichondrium. The samples were collected weekly, and cut into 100 μm thickness sections for SHG imaging. SHG imaging system was operated at 810 nm, producing SHG signals at half the excitation wavelength 405 nm. A Plan-Neofluar objective (x40 and NA=0.75) was employed for focusing the excitation beam into tissue samples and was also used to collect the backscattered intrinsic SHG signals. Our results showed apparent difference in collagen content and microstructure at various wound healing and scarring time points. It suggested that SHG signals from collagen can serve as a good indicator for characterization of wound status. With the advancement on miniaturization, microscopy based on SHG will become a valuable tool for monitoring the wound healing and scarring in vivo, and help to guide the improvement of scar appearance with appropriate and subtle modulation during wound healing based on better understanding of scarring response mechanism.

Paper Details

Date Published: 11 December 2012
PDF: 6 pages
Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 855322 (11 December 2012); doi: 10.1117/12.1000154
Show Author Affiliations
Yiyan Tang, Fujian Normal Univ. (China)
Fujian Medical Univ. (China)
Xiaoqin Zhu, Fujian Normal Univ. (China)
Shuyuan Xiong, Fujian Medical Univ. (China)
Jianxin Chen, Fujian Normal Univ. (China)

Published in SPIE Proceedings Vol. 8553:
Optics in Health Care and Biomedical Optics V
Qingming Luo; Ying Gu; Xingde D. Li, Editor(s)

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