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Journal of Biomedical Optics • Open Access

Automated alignment method for coherence-controlled holographic microscope
Author(s): Zbynek Dostal; Tomas Slaby; Lukas Kvasnica; Martin Lostak; Aneta Krizova; Radim Chmelik

Paper Abstract

A coherence-controlled holographic microscope (CCHM) was developed particularly for quantitative phase imaging and measurement of live cell dynamics, which is the proper subject of digital holographic microscopy (DHM). CCHM in low-coherence mode extends DHM in the study of living cells. However, this advantage is compensated by sensitivity of the system to easily become misaligned, which is a serious hindrance to wanted performance. Therefore, it became clear that introduction of a self-correcting system is inevitable. Accordingly, we had to devise a theory of a suitable control and design an automated alignment system for CCHM. The modulus of the reconstructed holographic signal was identified as a significant variable for guiding the alignment procedures. From this, we derived the original basic realignment three-dimensional algorithm, which encompasses a unique set of procedures for automated alignment that contains processes for initial and advanced alignment as well as long-term maintenance of microscope tuning. All of these procedures were applied to a functioning microscope and the tested processes were successfully validated. Finally, in such a way, CCHM is enabled to substantially contribute to study of biology, particularly of cancer cells in vitro.

Paper Details

Date Published: 28 October 2015
PDF: 8 pages
J. Biomed. Opt. 20(11) 111215 doi: 10.1117/1.JBO.20.11.111215
Published in: Journal of Biomedical Optics Volume 20, Issue 11
Show Author Affiliations
Zbynek Dostal, Brno Univ. of Technology (Czech Republic)
CEITEC- Central European Institute of Technology (Czech Republic)
Tomas Slaby, TESCAN ORSAY HOLDING, a.s. (Czech Republic)
Lukas Kvasnica, Brno Univ. of Technology (Czech Republic)
TESCAN ORSAY HOLDING, a.s. (Czech Republic)
Martin Lostak, TESCAN ORSAY HOLDING, a.s. (Czech Republic)
Aneta Krizova, CEITEC Brno Univ. of Technology (Czech Republic)
TESCAN ORSAY HOLDING, a.s. (Czech Republic)
Radim Chmelik, CEITEC Brno Univ. of Technology (Czech Republic)
Brno Univ. of Technology (Czech Republic)

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