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Journal of Biomedical Optics • Open Access

Multiplexed label-free optical biosensor for medical diagnostics
Author(s): Barbara Bottazzi; Lucia Fornasari; Ana Frangolho; Silvia Giudicatti; Alberto Mantovani; Franco Marabelli; Gerardo Marchesini; Paola Pellacani; Rita Therisod; Andrea Valsesia

Paper Abstract

This paper describes a new multiplexed label-free biosensor. The detection technology is based on nanostructured gold-polymer surfaces. These surfaces support surface plasmon resonance modes that can be probed by a miniaturized optical setup. The optical characterization of the sensing chip shows the sensitivity and the limit-of-detection to refractive index changes. Moreover, by studying the progressive adhesion of molecular monolayers of polyelectrolytes, the decay of the plasmonic mode electric field above the surface has been reconstructed. A multiplexed label-free biosensing device is then described and characterized in terms of sensitivity, lateral resolution, and sensitivity to a model biological assay. The sensitivity in imaging mode of the device is of the order of 10 −6 refractive index units, while the measured lateral resolution is 6.25 μm within a field of view of several tenths of mm 2 , making the instrument unique in terms of multiplexing capability. Finally, the proof-of-concept application of the technology as a point-of-care diagnostic tool for an inflammatory marker is demonstrated.

Paper Details

Date Published: 27 January 2014
PDF: 11 pages
J. Biomed. Opt. 19(1) 017006 doi: 10.1117/1.JBO.19.1.017006
Published in: Journal of Biomedical Optics Volume 19, Issue 1
Show Author Affiliations
Barbara Bottazzi, Fondazione Humanitas per la Ricerca (Italy)
Lucia Fornasari, Univ. degli Studi di Pavia (Italy)
Ana Frangolho, Plasmore S.r.l. (Italy)
Silvia Giudicatti, Univ. degli Studi di Pavia (Germany)
Alberto Mantovani, Fondazione Humanitas per la Ricerca (Italy)
Franco Marabelli, Univ. degli Studi di Pavia (Italy)
Gerardo Marchesini, Plasmore S.r.l. (Italy)
Paola Pellacani, Plasmore S.r.l. (Italy)
Rita Therisod, Univ. degli Studi di Pavia (Italy)
Andrea Valsesia, Plasmore S.r.l. (Italy)

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