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Spie Press Book

Optical Blood Flow Measurement in Microcirculatory Systems
Author(s): Laura Sironi; Laura D'Alfonso; Margaux Bouzin; Nicolo' Giovanni Ceffa; Maddalena Collini; Giuseppe Chirico; Pablo Blinder
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Book Description

This Spotlight reviews non-invasive optical methods to determine the blood flow velocity in microcirculation, with an emphasis on the most recent developments, that may be routinely applied in cellular biology laboratories. The methods covered in this book include both scanning and whole-field optical imaging techniques based on endogenous or exogenous (fluorescent) contrast agents for plasma and red-blood-cell visualization, such as laser Doppler velocimetry, laser speckle contrast imaging, diffuse correlation spectroscopy, particle image velocimetry, and two-photon microscopy. Several methods have been developed to quantify the blood flow velocity within microvessels by exploiting the cross-correlation concept applied to optical scanning microscopes: dual-foci cross-correlation, spatio-temporal image correlation spectroscopy, line-scanning techniques, and a recently developed single raster-scanned xy-image technique (FLICS). The basic operational principles, the advantages and disadvantages, and the technical issues of these methods will be discussed and compared, and their applications in biomedical research will be presented.

Book Details

Date Published: 30 December 2016
Pages: 98
ISBN: 9781510605008
Volume: SL23

Table of Contents
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1 Introduction

2 Laser Speckle Contrast Imaging
2.1 Principles
2.2 Instrument configuration and data acquisition
2.3 Applications

3 Doppler Optical Coherence Tomography
3.1 Principles
3.2 Instrument configuration and data acquisition
3.3 Applications

4 Diffuse Correlation Spectroscopy
4.1 Principles
4.2 Instrument configuration and data acquisition
4.3 Applications

5 Multiplexed and Image Correlation Methods: Video-Based Acquisition
5.1 Dual focus cross-correlation
     5.1.1 Principles
     5.1.2 Instrument configuration and data acquisition
5.2 Particle image velocimetry
     5.2.1 Principles
     5.2.2 Instrument configuration and data acquisition
5.3 Spatiotemporal image correlation spectroscopy
     5.3.1 Principles
     5.3.2 Instrument configuration and data acquisition
5.4 Applications
5.5 Other related methods

6 Space�Time Diagrams and Image Correlation Methods: Scanning-Based Acquisition and Detection
6.1 Line-scan and arbitrary-path blood flow measurements
     6.1.1 Principles
 Analysis of line-scan data using scanning laser image correlation
 Analysis of line-scan data using the Radon transform
6.2 Flow image correlation spectroscopy
     6.2.1 Principles
6.3 Instrument configuration and data acquisition
6.4 Applications



Microcirculation is a complex network of small vessels that deliver blood, nourishment, and molecules to tissues and organs, playing a crucial role in their maintenance and hemodynamic. Therefore, its impairment and dysfunction are involved in a variety of pathological processes. Moreover, the measurement of the blood flow velocity provides essential information about subject health, pathological conditions, diagnosis of diseases, and the development and control of the response to treatment. This Spotlight reviews the basic operational principles, advantages/drawbacks, and the applications to the biomedical research of different noninvasive optical methods to measure the blood flow velocity, based both on whole-field and scanning imaging techniques.

Laura Sironi
Laura D'Alfonso
Pablo Blinder
Margaux Bouzin
Nicolo Giovanni Ceffa
Maddalena Collini
Giuseppe Chirico
December 2016

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