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Spie Press Book

In Vivo Microscopy in the Male and Female Genital Tract
Author(s): Sabine Koelle; Matthias Trottmann
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Book Description

Using rodent and bovine subjects as models, this Spotlight focuses on the methodology and diagnostic value of three new imaging technologies to visualize the physiology and pathology of the male and female genital tract under in vivo conditions. These methods include digital video microscopy and probe-based laser endomicroscopy (pCLE).

Book Details

Date Published: 2 August 2016
Pages: 28
ISBN: 9781510604391
Volume: SL19

Table of Contents
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1 Introduction
1.1 Purpose
1.2 Establishing new techniques for in vivo microscopy in the male genital tract
1.3 Establishing new techniques for in vivo microscopy in the female genital tract

2 Digital Videomicroscopy
2.1 Fundamentals of live cell imaging using digital video microscopy
2.2 Preparation of tissue
2.3 Qualitative analysis
2.4 Quantitative analysis
2.5 Diagnostic value and clinical application

3 Probe-Based Confocal Laser Endomicroscopy
3.1 Fundamentals of pCLE
3.2 Preparation of tissues
3.3 Image acquisition and interpretation
3.4 Diagnostic value and clinical application



An in-depth understanding of the morphology and function of organs in the body is pivotal for any health professional. If health is compromised, successful intervention is only possible when the malfunctions can be identified, visualized, and monitored. The imaging technologies described in this Spotlight might contribute to add new diagnostic and therapeutic strategies in the daily routine of clinicians in the future. In the present, these technologies provide valuable tools for researchers to develop new and stunning insights into the physiology and pathology of reproductive biology - a pivotal prerequisite for achieving progress in the diagnosis and therapy of disorders in the reproductive tract.

This book is intended to provide new insights in live cell imaging for researchers and physicians working in andrology or gynecology and to stimulate the further development of imaging technologies in the field. As a precise, step-by-step instruction is provided, the book should enable interested researchers to apply these technologies for elucidating their special field of interest and to find answers to pertinent questions.

Sabine Koelle
Matthias Trottmann
July 2016

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