Digital Forum
Online Only
6 - 11 March 2021
Plenary Events
BiOS Hot Topics
Date: Saturday, 6 March 2021LIVE EVENT
Time: 12:00 PM - 2:00 PM PST
Location: Zoom
Open to all Photonics West paid conference attendees.

Welcome and Opening Remarks

BiOS 2021 Symposium Chair
Jennifer Barton, The Univ. of Arizona (United States)


BiOS 2021 Symposium Chair
Wolfgang Drexler, Medical Univ. of Vienna (Austria)


Presentation by 2021 Britton Chance Biomedical Optics Award Winner Enrico Gratton, Univ. of California Irvine (United States)

Hot Topics Facilitator Remarks

Sergio Fantini, Tufts Univ. (United States)

Breaking the status-quo of high-speed linear and nonlinear microscopy

Kevin Tsia, The Univ. of Hong Kong (Hong Kong)

Multimodal imaging: Photoacoustic imaging plus more

Chulhong Kim, Pohang Univ. of Science and Technology (Korea, Republic of)

Ophthalmic OCT--New Applications in its 4th decade

Michael Kaschke, Karlsruher Institut für Technologie (Germany)

Near-Infrared nerve-specific probes to guide surgery

Summer Gibbs, Oregon Health & Science Univ. (United States)

Translating spectroscopy for clinical care

Anita Mahadevan-Jansen, Vanderbilt Univ. (United States)

Skin-interfaced wireless wearables for physiological monitoring: Applications in syndromic tracking of COVID19

John Rogers, Northwestern Univ. (United States)

Understanding and detecting viruses with surface-enhanced Raman Spectroscopy

Laura Fabris Rutgers, The State Univ. of New Jersey (United States)

Towards intraoperative THz diagnosis of brain gliomas (high-impact article from SPIE Journal of Biomedical Optics)

Kirill Zaytsev, A.M. Prokhorov General Physics Institute of the RAS (Russia)
Neurotechnologies Plenary Session
Date: Sunday, 7 March 2021LIVE EVENT
Time: 2:30 PM - 4:30 PM PST
Location: Zoom
Times for this live event are all Pacific Standard Time (UTC-8 hours)

Open to all Photonics West paid conference attendees.



Welcome and Opening Remarks, Elizabeth Hillman, Columbia Univ. (USA), SPIE Brain Symposium Chair

Presentations:



Estimation of cerebral O2 consumption with 2-photon phosphorescence microscopy

Anna Devor, Boston Univ. (USA)

Abstract: The cerebral cortex is organized in cortical layers that differ in their cellular density, composition, and wiring. Cortical laminar architecture is also readily revealed by staining for cytochrome oxidase – the last enzyme in the respiratory electron transport chain located in the inner mitochondrial membrane. I has been hypothesized that a high-density band of cytochrome oxidase in cortical layer IV reflects higher oxygen consumption under baseline (unstimulated) conditions. Here, we test the hypothesis using direct measurements of the partial pressure of O2 (pO2) in cortical tissue obtained with 2-photon phosphorescence lifetime microscopy (2PLM). We revisit our previously developed method for extraction of cerebral metabolic rate of O2 (CMRO2) based on 2-photon pO2 measurements around diving arterioles and apply this method to estimate baseline CMRO2 in awake mice across cortical layers. Our results show that, in contrary to the common notion, baseline CMRO2 in layer IV is not higher than that in upper layers. We speculate that the cytochrome oxidase band in layer IV may reflect higher metabolic demands during transient surges in neuronal activity. These findings are important for interpretation of cortical-layer-resolved Blood Oxygen Level Dependent (BOLD) functional Magnetic Resonance Imaging (fMRI) studies.

Biography: Anna Devor received her initial research training at Hebrew University of Jerusalem, Israel. Her PhD thesis focused on biophysical mechanisms of the membrane potential oscillations in a network of electrically coupled neurons. After defending her PhD thesis in 2002, she went on to specialize in brain imaging at Martinos Center for Biomedical Imaging at MGH. In 2005, she established an independent research laboratory at UC San Diego. In 2019, she moved her laboratory to Boston University. Dr. Devor is a world expert in neurovascular and neurometabolic coupling in the brain and in regulation of cerebral blood flow.



Capture fast volumetric dynamics in the brain by light field microscopy

Kai Wang, Institute of Neuroscience, Chinese Academy of Sciences (China)

Abstract: Life is dynamic and three-dimensional (3D). An ideal tool to study biological processes, particularly those involving large populations of functionally diverse cells and fast dynamics, such as brains, is to achieve volumetric imaging in vivo. Traditional imaging tools, such as point scanning confocal microscope, are too slow to catch rapid processes over large scales. Volumetric imaging techniques that completely parallelized the imaging collection start attracting increasingly more attentions recently. Here, we report several new types of light field microscopes that can do functional imaging of calcium activities over large populations of neurons at high speed. We demonstrated whole brain functional imaging in freely behaving larval zebrafish and captured activation of several functional neural ensembles during different phases of larval zebrafish prey capture behavior. We also recorded populations of neurons’ activity and track fast circulating blood cells in 3D vascular network in the mouse brain. We believe these volumetric high speed imaging techniques could open new windows to understand more dynamic biological processes in vivo.

Biography: Kai Wang is a group leader in laboratory of Optical Neuroimaging in Institute of Neuroscience, Chinese Academy of Sciences. He received his B.S. and M.S. degrees at Tsinghua University (2005 and 2007) and Ph.D. at Harvard University (2011). After he finished postdoctoral training in Janelia Research Campuse, HHMI, he joined the Institute of Neuroscience in 2015 with major research interests on developing new fluorescent imaging techniques for neuroscience studies.



Cracking hypothalamic circuits that drive survival behaviors using novel optical methods

Yeka Aponte, National Institutes of Health and Johns Hopkins Univ. (USA)

Abstract: Functional imaging in vivo has become a powerful tool for studying neuronal circuits during behavior. Here we use a microendoscopy system for deep-brain imaging from two distinct lateral hypothalamic neuron populations (i.e. LHPV and LHLEPR). By combining endomicroscopy, genetically encoded calcium indicators, optogenetics, and chemogenetics, we demonstrate that LHPV neurons modulate pain whereas LHLEPR neuronal activity encodes for appetitive behaviors in mice.

Biography: Yeka Aponte is Chief of the Neuronal Circuit and Behavior Unit at the National Institute on Drug Abuse (NIH/NIDA-IRP). Adjunct Assistant Professor at Johns Hopkins University Neuroscience. Associate Director for Diversity and Inclusion at the NIDA-IRP. Her research aims to understand how genetically-identified cell types and their projections drive behaviors essential for survival.



Seeking new biomarkers with diffuse correlation spectroscopy and next generation devices for transcranial assessment of cerebral hemodynamics

Turgut Durduran, ICFO-The Institute of Photonic Sciences in Barcelona (Spain)

Abstract: I will first show the latest advances in testing diffuse correlation spectroscopy (DCS) in neuro-monitoring applications and how new biomarkers of cerebral health are emerging through improved technology. This will be illustrated through clinical examples from several studies in clinics. Then I will describe next generation devices which are pushing the technology towards in both the direction of regulatory approvals and also in low-cost, wearable implementations.

Biography: Turgut Durduran is an ICREA professor at ICFO-The Institute of Photonic Sciences in Barcelona (Spain). He leads the ICFO-Medical Optics group which works with academic, industry and clinics to develop and translate new optical technologies to clinical applications.



Talking to the brain in its own language: a new approach to treating blindness

Sheila Nirenberg, Cornell Univ. (USA)

Abstract: Neuroscience research has focused largely on listening to the brain - on taking recordings, analyzing responses, and trying to extract meaning from them. But now we’re entering a new phase where we can go beyond listening and can start talking back to the brain, and we have developed the ability to do it in the brain’s own language. This opens the door to new technologies for treating disease. Here we present the development of one such technology: a new kind of optogenetic prosthetic for treating blindness.

Biography: Sheila Nirenberg is a professor at the Weill Medical College of Cornell University and the founder of two companies – one that develops new kinds of prosthetic devices, and one that develops new kinds of smart robots. She has received numerous awards for her innovations, including a MacArthur award, and her work on cracking the retina’s neural code has been featured in TED talks, BBC’s “The Genius Behind”, and other documentaries, as well as many peer-reviewed journals.

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