The Moscone Center
San Francisco, California, United States
1 - 6 February 2020
Conference 11246
Single Molecule Spectroscopy and Superresolution Imaging XIII
Saturday - Sunday 1 - 2 February 2020
Conference
Committee
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Conference Chairs
Program Committee
Program Committee continued...
  • Ewa M. Goldys, The Univ. of New South Wales (Australia)
  • Zygmunt Karol Gryczynski, Univ. of North Texas Health Science Ctr. at Fort Worth (United States), Texas Christian Univ. at Fort Worth (United States)
  • Mike Heilemann, Goethe-Univ. Frankfurt am Main (Germany)
  • Johan Hofkens, KU Leuven (Belgium)
  • Zhen-Li Huang, Huazhong Univ. of Science and Technology (China)
  • Markus Sauer, Univ. Bielefeld (Germany)
  • Shimon Weiss, Univ. of California, Los Angeles (United States)
  • Andong Xia, Institute of Chemistry (China)

Additional Conference
Information
Chairs emeritus: Zygmunt Karol Gryczynski, Univ. of North Texas Health Science Ctr. at Fort Worth (United States)
Saturday 1 February Show All Abstracts
Welcome & Introduction
Saturday 1 February 2020
8:55 AM - 9:00 AM

Rainer Erdmann gives welcome and introduction
Session 1:
Biological or Multimodal Applications
Saturday 1 February 2020
9:00 AM - 10:20 AM
Session Chair:
Rainer Erdmann, PicoQuant GmbH (Germany)
Novel semiconductor-laser-integrated AFM active optical probe with ultrashort pulses and nanoscale aperture
Paper 11246-1
Author(s): Alexander A. Ukhanov, Fei-Hung Chu, Gennady A. Smolyakov, Kevin J. Malloy, Actoprobe LLC (United States)
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ElastoTweezers: A novel platform for high-precision cell elasticity measurements
Paper 11246-2
Author(s): Karsten Gall, Andy Sischka, Ionovation GmbH (Germany); Sebastian Knust, Univ. Bielefeld (Germany); Hendrik Milting, Herz- und Diabeteszentrum Nordrhein-Westfalen (Germany); Bastien Venzac, Séverine Le Gac, Univ. of Twente (Netherlands); Elwin Vrouwe, Micronit Microfluidics B.V. (Netherlands); Martina Viefhues, Dario Anselmetti, Univ. Bielefeld (Germany)
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Feedback enabled pinpoint force fluorescence microscope
Paper 11246-3
Author(s): Patrick Schmidt, Benjamin Reichert, John Lajoie, Iowa State Univ. of Science and Technology (United States); Sanjeevi Sivasankar, Univ. of California, Davis (United States)
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Super-resolution imaging of pathological tissue reveals higher-order chromatin folding in cancer development
Paper 11246-4
Author(s): Yang Liu, Univ. of Pittsburgh (United States)
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Session 2:
FLIM, FRET & FCS I
Saturday 1 February 2020
10:50 AM - 12:10 PM
Session Chair:
Mike Heilemann, Goethe-Univ. Frankfurt am Main (Germany)
Fluorescence lifetime image scanning microscopy
Paper 11246-5
Author(s): Ingo Gregor, Niels Radmacher, Jörg Enderlein, Georg-August-Univ. Göttingen (Germany)
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Scanning FCS and super-resolution microscopy on 2D lipid membranes
Paper 11246-6
Author(s): Uwe Ortmann, PicoQuant GmbH (Germany); Mariano Gonzalez Pisfil, PicoQuant GmbH (Germany), Humboldt-Univ. (Germany); Marcelle König, Rhys Dowler, Benedikt Krämer, Sumeet Rohilla, Felix Koberling, Rainer Erdmann, PicoQuant GmbH (Germany)
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Fast and compact Time-Correlated Single Photon Counting system for high-speed measurement with low distortion
Paper 11246-7
Author(s): Giulia Acconcia, Serena Farina, Ivan G. Labanca, Massimo Ghioni, Ivan Rech, Politecnico di Milano (Italy)
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Efficient wide-field FLIM and image modulation using Pockels cells
Paper 11246-8
Author(s): Adam Bowman, Mark A. Kasevich, Stanford Univ. (United States)
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Lunch Break 12:10 PM - 1:40 PM
Session 3:
FLIM, FRET & FCS II
Saturday 1 February 2020
1:40 PM - 3:10 PM
Session Chair:
Felix Koberling, PicoQuant GmbH (Germany)
Efficient light harvesting biotic-abiotic nanohybrid system incorporating atomically thin van Der Waals transition metal dichalcogenides (Invited Paper)
Paper 11246-9
Author(s): Mircea Cotlet, Mingxing Li, Brookhaven National Lab. (United States)
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Dual-color super-resolution imaging for FRET measurements: Energy transfer among donor/acceptor pairs of quantum dots
Paper 11246-10
Author(s): Duncan Ryan, Los Alamos National Lab. (United States); Megan K. Dunlap, Colorado State Univ. (United States); Somak Majumder, Chris J. Sheehan, James H. Werner, Jennifer A. Hollingsworth, Los Alamos National Lab. (United States); Martin P. Gelfand, Alan K. Van Orden, Colorado State Univ. (United States); Peter M. Goodwin, Los Alamos National Lab. (United States)
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Elucidation of the protein conformational changes occurring during assembly of P22 bacteriophage coat protein
Paper 11246-11
Author(s): Sanchari Banerjee, Carolyn Teschke, Univ. of Connecticut (United States)
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Ultrafast fluorescence lifetime imaging microscopy by frequency-division multiplexing
Paper 11246-12
Author(s): Hiroshi Kanno, The Univ. of Tokyo (Japan); Hideharu Mikami, The Univ. of Tokyo (Japan), Japan Science and Technology Agency (Japan); Keisuke Goda, The Univ. of Tokyo (Japan), Univ. of California (United States), Wuhan Univ. (China)
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Session 4:
Nanoscopy and Superresolution Microscopy I
Saturday 1 February 2020
3:40 PM - 5:30 PM
Session Chair:
Ingo Gregor, Georg-August-Univ. Göttingen (Germany)
The phasor FLIM analysis reveals metabolic modulation and cell migration through adhesion-mediated contractility (Invited Paper)
Paper 11246-13
Author(s): Michelle Digman, Austin Lefebvre, Emma Mah, Albert Yee, Univ. of California, Irvine (United States)
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Planar plasmonic antenna arrays resolve transient nanoscopic heterogeneities in biological membranes
Paper 11246-14
Author(s): Pamina Winkler, ICFO - Institut de Ciències Fotòniques (Spain)
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Microsecond transient absorption spectroscopy at 77 K to inform cryogenic single-molecule active control microscopy
Paper 11246-15
Author(s): Annina M. Sartor, Peter D. Dahlberg, William E. Moerner, Stanford Univ. (United States)
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Achieving spontaneous super-resolution in a confocal microscope by exploiting super-linear emitters
Paper 11246-16
Author(s): Denitza Denkova, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia), Macquarie Univ. (Australia), Institute for Bioengineering of Catalonia (Spain); Martin Ploschner, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia), Macquarie Univ. (Australia), The Univ. of Queensland (Australia); Minakshi Das, Lindsay M. Parker, Xianlin Zheng, Yiqing Lu, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia), Macquarie Univ. (Australia); Antony Orth, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia), RMIT Univ. (Australia); Nicolle H. Packer, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia), Griffith Univ. (Australia); James A. Piper, ARC Ctr. of Excellence for Nanoscale BioPhotonics (Australia), Macquarie Univ. (Australia)
Show Abstract
Combining 3D single molecule localization strategies and sprectral demultiplexing for bioimaging
Paper 11246-17
Author(s): Clément Cabriel, Nicolas Bourg, Pierre Jouchet, Guillaume Dupuis, Institut des Sciences Moléculaires d'Orsay (France); Cyndelia Guillaume, Abbelight (France); Aurélie Baron, Institut de Chimie des Substances Naturelles (France); Marie-Ange Badet-Denisot, Ctr. de Recherche de Gif-sur-Yvette (France); Boris Vauzeilles, Institut de chimie moléculaire et des matériaux d'Orsay (France); Christophe Leterrier, Aix-Marseille Univ. (Finland); Emmanuel Fort, Institut Langevin Ondes et Images (France); Sandrine Leveque-Fort, Institut des Sciences Moléculaires d'Orsay (France)
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Session HT:
BiOS Hot Topics
Saturday 1 February 2020
7:00 PM - 9:00 PM
Sunday 2 February Show All Abstracts
Session 5:
New Fundamental Single Molecule Techniques I
Sunday 2 February 2020
8:50 AM - 10:10 AM
Session Chair:
Mike Heilemann, Goethe-Univ. Frankfurt am Main (Germany)
Highly inclined plane illumination microscopy with extended imaging depth
Paper 11246-18
Author(s): Jinhan Ren, Kyu Young Han, CREOL, The College of Optics and Photonics, Univ. of Central Florida (United States)
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Line-scanning confocal microscopy with multiple inclined beams
Paper 11246-19
Author(s): Jialei Tang, Kyu Young Han, CREOL, The College of Optics and Photonics, Univ. of Central Florida (United States)
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Correlating DNA-PAINT and single-molecule FRET for multiplexed super-resolution imaging
Paper 11246-20
Author(s): Nina S. Deussner-Helfmann, Goethe-Univ. Frankfurt am Main (Germany); Alexander Auer, Maximilian T. Strauss, Max-Planck-Institut für Biochemie (Germany), Ludwig-Maximilians-Univ. München (Germany); Paul Donlin-Asp, Max-Planck-Institut für Hirnforschung (Germany); Sebastian Malkusch, Marina S. Dietz, Hans-Dieter Barth, Goethe-Univ. Frankfurt am Main (Germany); Erin Schuman, Max-Planck-Institut für Hirnforschung (Germany); Ralf Jungmann, Max-Planck-Institut für Biochemie (Germany), Ludwig-Maximilians-Univ. München (Germany); Mike Heilemann, Goethe-Univ. Frankfurt am Main (Germany)
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Self-interference digital holography (SIDH) for far-field three-dimensional nanoscale localization of fluorescent nanoparticles
Paper 11246-21
Author(s): Abhijit Marar, Peter Kner, Univ. of Georgia (United States)
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Session 6:
Nanoscopy and Superresolution Microscopy II
Sunday 2 February 2020
10:40 AM - 12:10 PM
Session Chair:
Ingo Gregor, Georg-August-Univ. Göttingen (Germany)
Quantitative single-molecule localization microscopy reports on protein numbers in signaling protein complexes (Invited Paper)
Paper 11246-22
Author(s): Mike Heilemann, Goethe-Univ. Frankfurt am Main (Germany)
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Imaging the photoemission of CdSe/CdS quantum dot clusters with sub-nanosecond time resolution and nanometer scale spatial precision (LA-UR-19-27019)
Paper 11246-23
Author(s): Megan K. Dunlap, Colorado State Univ. (United States); Duncan Ryan, Peter M. Goodwin, James H. Werner, Somak Majumder, Jennifer A. Hollingsworth, The Ctr. for Integrated Nanotechnologies (United States), Los Alamos National Lab. (United States); Martin P. Gelfand, Alan K. Van Orden, Colorado State Univ. (United States)
Show Abstract
Quantum imaging with SPAD arrays
Paper 11246-24
Author(s): Gur Lubin, Ron Tenne, Weizmann Institute of Science (Israel); Ivan Michel Antolovic, Edoardo Charbon, Claudio Bruschini, Ecole Polytechnique Fédérale de Lausanne (Switzerland); Dan Oron, Weizmann Institute of Science (Israel)
Show Abstract
3D single molecule localization microscopy based on time modulated illumination.
Paper 11246-25
Author(s): Pierre Jouchet, Clément Cabriel, Nicolas Bourg, Marion Bardou, Institut des Sciences Moléculaires d'Orsay (France); Christian Poüs, Institut Paris Saclay d’Innovation Thérapeutique (France); Emmanuel Fort, Institut Langevin Ondes et Images (France); Sandrine Leveque-Fort, Institut des Sciences Moléculaires d'Orsay (France)
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Lunch Break 12:10 PM - 1:40 PM
Session 7:
Nanoscopy and Superresolution Microscopy III
Sunday 2 February 2020
1:40 PM - 3:00 PM
Session Chair:
Felix Koberling, PicoQuant GmbH (Germany)
Towards easier, faster, super-resolved microscopy (Invited Paper)
Paper 11246-26
Author(s): Paul M. W. French, Imperial College London (United Kingdom)
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MINFLUX: Achieving the ultimate resolution limit in fluorescence microscopy (Invited Paper)
Paper 11246-27
Author(s): Francisco Balzarotti, Research Institute of Molecular Pathology (Austria); Jasmin K. Pape, Stefan W. Hell, Max-Planck-Institut für Biophysikalische Chemie (Germany)
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Two-color two-photon excitation STED (2C2P-STED) microscopy
Paper 11246-28
Author(s): Christoph Polzer, Stefan Ness, Thomas Kellerer, Markus Hilleringmann, Hochschule für Angewandte Wissenschaften München (Germany); Joachim O. Raedler, Ludwig-Maximilians-Univ. München (Germany); Thomas Hellerer, Hochschule für Angewandte Wissenschaften München (Germany)
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Young Investigator Award
Sunday 2 February 2020
3:00 PM - 3:15 PM
Session Chair:
Ingo Gregor, Georg-August-Univ. Göttingen (Germany)
Session 8:
New Fundamental Single Molecule Techniques II
Sunday 2 February 2020
3:45 PM - 5:05 PM
Session Chair:
Paul M. W. French, Imperial College London (United Kingdom)
Super-resolution microscopy of / with luminescent carbon nanotubes for high-resolution brain imaging in the near-infrared
Paper 11246-29
Author(s): Antoine Godin, Chiara Paviolo, Noémie Danné, Antony Lee, Joana Ferreira, Laurent Groc, Laurent Cognet, Univ. de Bordeaux, CNRS (France)
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Imaging 3D orientation and wobbling of single fluorescent emitters by polarized super-resolved microscopy
Paper 11246-30
Author(s): Valentina Curcio, Caio Vaz Rimoli, Institut Fresnel (France); Cesar Augusto Valades Cruz, Institut Curie (France); Pascal Verdier-Pinard, Ctr. de Recherche en Cancérologie de Marseille (France); Manos Mavrakis, Sophie Brasselet, Institut Fresnel (France)
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Interferometric scattering for fluorescence-free electrokinetic trapping of single nanoparticles in free solution
Paper 11246-31
Author(s): Abhijit A. Lavania, Stanford Univ. (United States); Allison H. Squires, Stanford Univ. (United States), The Univ. of Chicago (United States); Peter D. Dahlberg, William E. Moerner, Stanford Univ. (United States)
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Surface enhanced Raman spectroscopy applied for anti-retroviral drug screening
Paper 11246-32
Author(s): Setumo Lebogang Thobakgale, Sello L. Manoto, Patience T. Mthunzi-Kufa, Rudzani Malabi, CSIR National Laser Ctr. (South Africa)
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Session PSun:
Posters-Sunday
Sunday 2 February 2020
5:30 PM - 7:00 PM

Conference attendees are invited to attend the BiOS poster session on Sunday evening. Come view the posters, enjoy light refreshments, ask questions, and network with colleagues in your field.

Poster Setup: Sunday 10:00 AM – 4:30 PM
View poster presentation guidelines and set-up instructions at
http://spie.org/PWPosterGuidelines
Temporal resolution enhancement in super-resolution imaging via auto-correlation and two-step deconvolution
Paper 11246-33
Author(s): Weisong Zhao, Yixuan Zhao, Jian Liu, Harbin Institute of Technology (China); Changliang Guo, Univ. of California, Los Angeles (United States); Haoyu Li, Harbin Institute of Technology (China)
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Determining the ending time of super-resolution localization microscopy experiments
Paper 11246-34
Author(s): Zhen-Li Huang, Wuhan National Lab. for Optoelectronics (China)
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Single molecule tracking and spectroscopy for investigating dynamics and interactions of neurotrophin receptors
Paper 11246-36
Author(s): Laura Marchetti, Scuola Normale Superiore (Italy), Univ. di Pisa (Italy); Fulvio Bonsignore, Chiara Schirripa Spagnolo, Francesco Gobbo, Scuola Normale Superiore (Italy); Rosy Amodeo, Scuola Normale Superiore (Italy), Ctr. per l'Innovazione delle Nanotecnologie, Istituto Italiano di Tecnologia (Italy); Fabio Beltram, Scuola Normale Superiore (Italy), Fondazione Pisana per la Scienza (Italy); Antonino Cattaneo, Stefano Luin, Scuola Normale Superiore (Italy)
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Measuring G-protein activation with spectrally resolved fluorescence fluctuation spectroscopy
Paper 11246-37
Author(s): Daniel J. Foust, David W. Piston, Washington Univ. in St. Louis (United States)
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An LED-based super resolution digital image scanning microscope
Paper 11246-38
Author(s): Yigit Uysalli, Ekin Özgönül, Fatmanur Tiryaki, Elif Nur Fırat Karalar, Alper Kiraz, Koç Univ. (Turkey)
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Measuring localization confidence for quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy
Paper 11246-39
Author(s): Hesam Mazidi, Tianben Ding, Arye Nehorai, Matthew D. Lew, Washington Univ. in St. Louis (United States)
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Quantification of labelled target molecules via dSTORM localization microscopy
Paper 11246-40
Author(s): Dániel Varga, Hajnalka Majoros, Zsuzsanna Ujfaludi, Tibor Pankotai, Miklos Erdélyi, Univ. of Szeged (Hungary)
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Super resolution infrared photothermal microscopy: operating principle and detection limits
Paper 11246-41
Author(s): Ilia M. Pavlovetc, Univ. of Notre Dame (United States); Eduard A. Podshivaylov, M.V. Lomonosov Moscow State Univ. (Russian Federation); Gregory V. Hartland, Univ. of Notre Dame (United States); Pavel A. Frantsuzov, M.V. Lomonosov Moscow State Univ. (Russian Federation); Masaru K. Kuno, Univ. of Notre Dame (United States)
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EMCCDs beyond 20MHz: For high speed applications
Paper 11246-44
Author(s): Yoann Gosselin, Jérémy Turcotte, Marie-Eve Ducharme, Olivier Daigle, Nüvü Cameras Inc. (Canada)
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Nano-resolution in vivo 3D orbital tracking system to study cellular dynamics and bio-molecular processes
Paper 11246-45
Author(s): Ulas C. Coskun, ISS, Inc. (United States); Matthew L. Ferguson, Huynh Anh, Boise State Univ. (United States); Alexander V. Less, Univ. of California, Irvine (United States); Yuansheng Sun, Shih-Chu J. Liao, Sunil Shah, ISS, Inc. (United States); Enrico Gratton, Univ. of California, Irvine (United States); Beniamino Barbieri, ISS, Inc. (United States)
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A single-molecule assay to measure protein binding affinity beyond photobleaching
Paper 11246-46
Author(s): Thilini Perera, Ying S. Hu, Univ. of Illinois at Chicago (United States); Wendi Fu, Univ. of Illinois (United States)
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A computationally-efficient bound for the variance of measuring the orientation of single molecules
Paper 11246-35
Author(s): Tingting Wu, Tianben Ding, Hesam Mazidi, Matthew D. Lew, Washington Univ. in St. Louis (United States)
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Line-scanning confocal fluorescence microscopy with improved spatial resolution
Paper 11246-42
Author(s): Vahid Ebrahimi, Chun-Hung Weng, Kyu Young Han, CREOL, The College of Optics and Photonics, Univ. of Central Florida (United States)
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Synthetic aperture localization nanoscopy
Paper 11246-43
Author(s): Jeffrey J. Field, Randy A. Bartels, Colorado State Univ. (United States)
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Activation and cross-interaction of receptor tyrosine kinases studied by single-molecule localization microscopy
Paper 11246-48
Author(s): Marie-Lena I. E. Harwardt, Sebastian Malkusch, Mark S. Schröder, Petra Freund, Goethe-Univ. Frankfurt am Main (Germany); Sebastian Strauss, Ralf Jungmann, Ludwig-Maximilians-Univ. München (Germany), Max-Planck-Institut für Biochemie (Germany); Marina S. Dietz, Mike Heilemann, Goethe-Univ. Frankfurt am Main (Germany)
Show Abstract
Correlating DNA-PAINT and single-molecule FRET for multiplexed super-resolution imaging
Paper 11246-49
Author(s): Nina S. Deussner-Helfmann, Goethe-Univ. Frankfurt am Main (Germany); Alexander Auer, Maximilian T. Strauss, Max-Planck-Institut für Biochemie (Germany), Ludwig-Maximilians-Univ. München (Germany); Paul Donlin-Asp, Max-Planck-Institut für Hirnforschung (Germany); Sebastian Malkusch, Marina S. Dietz, Hans-Dieter Barth, Goethe-Univ. Frankfurt am Main (Germany); Erin Schuman, Max-Planck-Institut für Hirnforschung (Germany); Ralf Jungmann, Max-Planck-Institut für Biochemie (Germany), Ludwig-Maximilians-Univ. München (Germany); Mike Heilemann, Goethe-Univ. Frankfurt am Main (Germany)
Show Abstract
Session PLN:
BiOS Sunday Plenary
Sunday 2 February 2020
7:15 PM - 8:00 PM
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