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Recent progress in using polarized light and Optical Angular Momentum for biomedical diagnostics at both tissue and cellular levels is attracting academic and industrial interest. The development and clinical implementation of new techniques, as well as translation of approaches developed by other scientific communities working in the fields of ellipsometry, astrophysics, photoelastography, etc. to the medical field can broaden the diagnostics toolkit of healthcare professionals.

This conference targets new methodologies and findings of polarized light and Optical Angular Momentum for in vitro, pre-clinical and clinical applications for biomedical diagnostics. A special focus of the conference is on the translation of polarimetric modalities to clinical settings. Scientific papers are solicited related to advanced polarimetric instrumentation, modeling, and applications of polarized light that push beyond the current state of sensitivity, specificity and applicability of different biomedical fields. These include:

Properties of tissues Novel polarimetric Techniques and Methods Biomedical applications of polarized light ;
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Conference 11963

Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2022

In person: 22 - 23 January 2022
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  • 1: Hot Topics in Polarization and Angular Momentum
  • 2: Pre-Clinical Applications of Polarimetry I
  • 3: Pre-Clinical Applications of Polarimetry II
  • 4: Clinical Applications of Polarimetry
  • 5: Microscopy and OCT I
  • 6: Microscopy and OCT II
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POST-DEADLINE ABSTRACT SUBMISSIONS

  • Submissions are accepted through 06-December
  • Notification of acceptance by 20-December

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Session 1: Hot Topics in Polarization and Angular Momentum
11963-30
To be announced (Invited Paper)
Author(s): Federico Capasso, Harvard John A. Paulson School of Engineering and Applied Sciences (United States)
11963-31
Author(s): Martin J. Booth, Chao He, Univ. of Oxford (United Kingdom)
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Sensitive vectorial imaging, such as through polarization microscopes, requires both high spatial resolution and high-fidelty polarimetry. We present new methods to enhance such approaches. We show new polarimetry that maps all analyser states into a single vectorially structured light field, permitting single-shot vectorial state analysis through physical model based inference. These methods avoid complex processing steps and error sources. We further introduce a new adaptive optics technology that can correct both phase and polarisation aberrations within optical systems. We validate improvements in both vector field state and the focal quality, through correction for commonplace sources of vectorial aberration.
11963-1
Author(s): Olga Korotkova, Univ. of Miami (United States); Jyrki Laatikainen, Ari Friberg, Tero Setälä, Univ. of Eastern Finland (Finland)
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Interaction of light with extended biological tissues involves continuous changes in its coherence and polarization states. Therefore, the classic Stokes-Mueller calculus based on the local (single-point) light transformation on the order of intensity (not field) cannot be used for complete and unique reconstruction of the optical properties of random, birefrigent bio-tissues. We suggest a generalization of the Stokes-Mueller calculus to two-point field correlations in which both the Stokes vector and the Mueller operator remain real-valued. We show in particular that the classic Poincare construction enjoyed by the single-point polarization state can be extended to two points to characterize, in addition to the polarization state, its degree of electromagnetic coherence and its degree of cross-polarization. The two-point Stokes-Mueller calculus can be used in a number of applications, e.g. spatial light modulator analysis but is envisioned to be of particular interest in biophotonics.
11963-2
Author(s): Omar Roriguez-Nunez, Lab. de Physique des Interfaces et des Couches Minces (France), CNRS (France), Ecole Polytechnique (France); Philippe Schucht, Inselspital, Univ. Bern (Switzerland); Ekkehard Hewer, CHUV, Ctr. Hospitalier Univ. Vaudois (Switzerland), Univ. de Lausanne (Switzerland); Tatiana Novikova, Angelo Pierangelo, Lab. de Physique des Interfaces et des Couches Minces (France), CNRS (France), Ecole Polytechnique (France)
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Imaging Mueller polarimetry (IMP) was used in reflection geometry and large field of view configuration for the in-plane visualization of brain fiber tracts by exploring the anisotropy of the refractive index of healthy brain white matter. Our initial studies demonstrated that IMP successfully detects in-plane orientation of fiber tracts on a flat surface of the excised brain specimens. This work, performed ex-vivo on complete fresh calf brains proves the potential of IMP as a technique suitable to detect both presence and orientation of brain fiber tracts in the adverse conditions of complex surface topography and presence of blood.
11963-3
Author(s): Joseph Chue-Sang, Thomas A. Germer, National Institute of Standards and Technology (United States)
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Alignment of tissue fibers, such as collagen, can be disrupted by some cancers. Mueller matrix polarimetry (MMP) is often correlated to this tissue orientation, as seen in cases such as cervical imaging. However, its bulk assessment of a sample’s polarimetric properties prevents it from distinguishing specific structures at different depths, as there would be in any non-uniform or multilayered sample. Spatial Frequency Domain Imaging (SFDI) is a well-documented technique that can be used to manipulate depth of penetration of an investigating light source through use of different sinusoidal frequency patterns. We have combined the two imaging modalities to investigate and differentiate between anisotropic samples with varied depth-dependent structure and demonstrate its use with various tissue phantoms. We also discuss some issues that arise with the combined methodology.
11963-4
Author(s): Leanne Iannucci, Matthew B. Riak, Spencer P. Lake, Washington Univ. in St. Louis (United States)
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Polarimetry has been used to characterize collagen fiber alignment in biological tissues, providing insights into structure-function relationships during unique loading conditions or in disease. These imaging modalities leverage the birefringence of collagen to describe fiber strength and orientation. Structural anisotropy is thought to be the primary driver of this optical phenomenon; however, the degree to which other extracellular matrix (ECM) properties (i.e., local density, crosslinking) confound polarimetry data has yet to be explored. In this study, the effects of individual ECM properties on polarized light propagation in transmission and diffuse reflectance modes was explored using a tunable collagen hydrogel system.
Session 2: Pre-Clinical Applications of Polarimetry I
11963-5
Author(s): Carla Rodríguez, Albert Van Eeckhout, Laia Ferrer, Univ. Autònoma de Barcelona (Spain); Enrique Garcia-Caurel, Lab. de Physique des Interfaces et des Couches Minces (France), CNRS (France), Ecole Polytechnique (France); Emilio González-Arnay, Univ. Autónoma de Madrid (Spain), Hospital Univ. de Canarias, Santa Cruz de Tenerife (Spain); Juan Campos, Angel Lizana, Univ. Autònoma de Barcelona (Spain)
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We propose a predictive method for biological tissue recognition based on the experimental Mueller matrix measurements of a collection of 157 ex-vivo chicken samples corresponding to four different tissue sets: bone, tendon, muscle and myotendinous junction. We highlight the potential of the predictive algorithm, built by means of the statistical analysis of different polarimetric observables that retrieve complete polarimetric information of samples (that is, dichroism, retardance and depolarization). Composed by four independent functions, the proposed predictive optical-based algorithm is capable to recognize in a single measurement, whether a random sample corresponds, or not, to one of the mentioned tissue groups.
11963-6
Author(s): Danielle J. Harper, Yongjoo Kim, Wellman Ctr. for Photomedicine (United States), Harvard Medical School (United States); Alejandra Gómez-Ramírez, Wellman Ctr. for Photomedicine (United States), Univ. Nacional de Colombia Sede Medellín (Colombia); Ahhyun Stephanie Nam, Wellman Ctr. for Photomedicine (United States), Harvard Medical School (United States); Benjamin J. Vakoc, Wellman Ctr. for Photomedicine (United States), Harvard Medical School (United States), Harvard-MIT Health Sciences and Technology (United States)
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Radiation concerns preclude the use of fluoroscopy guidance in lumbar puncture procedures performed on pregnant women or other at-risk groups. We have developed an alternative method for guidance based on a simple, unscanned polarization-sensitive optical coherence tomography needle tip probe. Using the porcine spine as a model, we show that the polarization signals returned by the probe allow each layer from the skin to the subarachnoid space to be uniquely identified in situ. Combining these signals with needle-tip tracking using Doppler methods provides real-time anatomical localization of the needle tip.
Session 3: Pre-Clinical Applications of Polarimetry II
11963-11
Author(s): Ben E. Urban, Colgate-Palmolive Co. (United States); Steven L. Jacques, Univ. of Washington (United States); Hrebesh Subhash, Colgate-Palmolive Co. (United States)
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Reflectance imaging gives a convolved image of superficial and deeper tissue layers. On the other hand, polarization reflectance imaging allows separation of superficial tissue from the convolved tissue image. In this report, polarized light imaging is used to investigate hydration and desiccation on superficial layers of ex vivo porcine skin tissues. A polarization camera acquired co-polarized and cross-polarized reflectance images from porcine front and back dermal surfaces. Polarized scattering is sensitive to sub-micrometer changes in tissue structure, and therefore is useful in detecting collagen density changes in tissue. The reflectance images were acquired at five different wavelengths (405, 490, 590, 660, and 700 nm) for hydrated and desiccated tissues. The back surface (dermal surface) of the skin was affected by hydration or desiccation, while the front surface (epidermal surface) was not as affected due to the stratum corneum which resists desiccation.
11963-12
Author(s): Deyan Ivanov, Lab. de Physique des Interfaces et des Couches Minces (France); Viktor Dremin, Aston Univ. (United Kingdom), Research & Development Ctr. of Biomedical Photonics (Russian Federation); Tsanislava Genova, Institute of Electronics, BAS (Bulgaria); Alexander Bykov, Univ. of Oulu (Finland); Igor Meglinski, Aston Univ. (United Kingdom), Univ. of Oulu (Finland), Institute of Clinical Medicine N.V. Sklifosovsky, I.M. Sechenov Moscow State Medical Univ. (Russian Federation); Tatiana Novikova, Lab. de Physique des Interfaces et des Couches Minces (France), Florida International Univ. (United States); Razvigor Ossikovski, Lab. de Physique des Interfaces et des Couches Minces (France)
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We present a combination of Mueller matrix measurements (635 nm) of cancerous colon specimens and machine-learning approach. Physical realizability filtering and symmetric decomposition were used to extract polarimetric quantities, used as predictors in machine-learning algorithms. The results were visualized using various depolarization spaces. Principal component analysis was used to extract particular features from the model, logistic regression evaluated predictors with high likelihood for tumor detection, while random forest and support vector machines provided the best results for classification. Hence, polarimetry combined with machine-learning approach may increase the histopathology diagnostic accuracy.
11963-15
Author(s): Oriol Arteaga, Dale Gottlieb, Sara Aguado, Adolf Canillas, Esther Pascual, Jordi Gomis-Bresco, Univ. de Barcelona (Spain)
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This work describes a wide-field imaging Mueller polarimetric system optimized for operation in the NIR range. It makes use of two fast switching compensators with optimal values of retardance that are oriented to a discrete set of orientations. The acquisition of 16 raw intensity images is done in a few seconds, which allows the determination of the complete Mueller matrix of a sample in the backscattering configuration at a speed compatible with in-vivo applications. Mueller matrix NIR imaging of polarization properties unveils quantitative information from deeper parts of the tissue than in systems using visible radiation, making it an interesting and promising tool for non-invasive biomedical diagnostic.
Session 4: Clinical Applications of Polarimetry
11963-14
Author(s): Tananant Boonya-Ananta, Mariacarla Gonzalez, Vinh Nguyen Du Le, Florida International Univ. (United States); Edward DeHoog, Optical Design & Engineering (United States); Michael J. Paidas, Arumugam Jayakumar, Univ. of Miami (United States); Jessica C. Ramella-Roman, Florida International Univ. (United States)
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Preterm birth (PTB), defined as any birth prior to 37 weeks of gestation, contributes to 35% of 3.1 million neonatal deaths annually. There is an absence of clinical tools available for accurate and early detection of preterm birth risks. We have developed a Portable PReterm IMaging System capable of 4x3 MMI which can be used at the point of care for monitoring of the cervix. The system was tested on a Gynecologic skills trainer as well as healthy volunteers showing image quality comparable to clinical colposcopes.
11963-16
Author(s): Hee Ryung Lee, Lab. de Physique des Interfaces et des Couches Minces (France), CNRS (France), Ecole Polytechnique (France); Ilyas Saytashev, Vinh Nguyen Du Le, Florida International Univ. (United States); Mala Mahendroo, The Univ. of Texas Southwestern Medical Ctr. at Dallas (United States); Jessica C. Ramella-Roman, Florida International Univ. (United States), Herbert Wertheim College of Medicine, Florida International Univ. (United States); Tatiana Novikova, Lab. de Physique des Interfaces et des Couches Minces (France), Florida International Univ. (United States), CNRS (France)
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Thin sections of uterine cervices from pregnant mice at day 6 and 18 of 19-days gestation period at different spatial locations along the cervices were studied with imaging Mueller polarimetry (IMP) combined with statistical analysis and multi-curve fit. The results suggest using depolarization and linear retardance images for collagen scoring and identification of cervical collagen changes during pregnancy. One day before delivery the remodeling of extracellular matrix of cervical collagen was detectable at the external cervical os, thus, proving that IMP modality may serve for the preterm birth risk assessment associated with the accelerated remodeling of cervical collagen.
Session 5: Microscopy and OCT I
11963-21
Author(s): Jigar Lad, Stefano Serra, Jillian Sprenger, Georgia Thomas, Blake Jones, Fayez Quereshy, Univ. of Toronto (Canada); Mohammadali Khorasani, The Univ. of British Columbia (Canada); Alex Vitkin, Univ. of Toronto (Canada)
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Mounting evidence supports the prognostic value contained within the tumour stromal architecture, more specifically the desmoplastic response (DR). However, despite its promise, DR suffers from inter-observer variability, making its clinical uptake difficult. In this study, we are applying our novel polarized light microscopy technique, combined with advanced texture analysis and machine learning algorithms, to offer a quantitative assessment of DR and to investigate the potential prognostic capabilities of our approach. The overarching goal of this study is to expand on our polarized light imaging + quantification methodology, as a step towards improved cancer stratification and personalized therapy management.
11963-22
Author(s): Sophie Brasselet, Miguel Sison, Institut Fresnel (France)
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Polarized nonlinear imaging is an important tool to image molecular organization in tissues using intrinsic nonlinear or multiphoton contrasts. The dynamics of polarized nonlinear imaging is however today still limited to seconds time rates. We have developed an interferometric frequency shift-based method using Acousto-Optic Modulation, capable of reaching sub-second dynamics in polarized scanning imaging. The method is applied to real time structural imaging in cells and tissues.
11963-23
Author(s): Ludovick Bégin, Brain Research Ctr., Ctr. de Recherche CERVO (Canada); Damon DePaoli, Wellman Ctr. for Photomedicine (United States); Brett Bouma, Wellman Ctr. for Photomedicine, Harvard Medical School (United States); Daniel C. Côté, Brain Research Ctr., Ctr. de Recherche CERVO (Canada); Martin Villiger, Wellman Ctr. for Photomedicine (United States)
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Precancer-to-cancer progression in epithelial tissues is marked by cellular proliferation and nuclear pleomorphism. These transformations have been shown to influence tissue optical properties: (1) tissue turbidity (quantified by the scattering coefficient) and (2) average scatterer size. It is found that backscattered polarized light can assess these properties. We use linear and circular depolarization to generate a predictive response surface where turbid media samples cluster by scatterer size and scattering coefficient. The novel polarimetric ability to noninvasively and simultaneously assess scatterer size and scattering coefficient of tissue-like turbid media may improve upon currently invasive skin cancer tests.
Session 6: Microscopy and OCT II
11963-25
Author(s): Lida Zhu, Shuichi Makita, Daisuke Oida, Univ. of Tsukuba (Japan); Arata Miyazawa, Sky Technology Inc. (Japan); Kensuke Oikawa, Pradipta Mukherjee, Univ. of Tsukuba (Japan); Antonia Lichtenegger, Univ. of Tsukuba (Japan), Zentrum für Medizinische Physik und Biomedizinische Technik, Medizinische Univ. Wien (Austria); Yoshiaki Yasuno, Univ. of Tsukuba (Japan)
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A three-dimensional label-free multi-contrast imaging for ex vivo tissue investigation is presented. Computational refocusing is implemented in a Jones-matrix polarization-sensitive optical coherence tomography (PS-OCT) system to overcome the trade-off between imaging depth and lateral resolution. The application of multiple contrast imaging, including intensity, birefringence, and degree-of-polarization uniformity (DOPU), is demonstrated by phantom, porcine muscle, and zebrafish measurements. Extended imaging depth with enhanced lateral resolution over millimeter is achieved. In tissue imaging we find some altered birefringence and DOPU estimation, whose size and alteration are proportional to defocus amount. This biased estimation can be numerically mitigated after applying computational refocusing.
11963-28
Author(s): Mariacarla Gonzalez, Camilo Roa, Florida International Univ. (United States); Arturo Jimenez, Univ. of Central Florida (United States); Rachelle Gomez-Guevara, Univ. of Rochester (United States); V.N. Du Le, Jessica C. Ramella-Roman, Florida International Univ. (United States)
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One million infants will die of complications from reduced gestation before the age of five. Monitoring elastin and collagen arrangement in the uterine cervix interests researchers studying preterm birth. We have utilized a Self-validating Mueller matrix micro-mesoscope (SAMMM) with convolutional neural networks and K-nearest neighbor for classification of the fibers in the mouse cervix. A polarized microscope can be used for fiber classification leveraging the developed classifier. The Mueller matrix and decomposition parameters from excised cervical tissues are fed to the previously developed classifier. This low cost tool, powered with machine learning, could improve access to collagen and elastin imaging.
11963-29
Author(s): Mariia A. Borovkova, Ivan Lopushenko, Oleksii Sieryi, Alexander Bykov, Univ. of Oulu (Finland); Igor Meglinski, Aston Univ. (United Kingdom)
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The structural alterations in biological tissues influence the tissue optical properties such as scattering, birefringence and optical activity. We developed a Stokes vector polarimetric system to investigate the peculiarities of the phase change of circularly polarized light during its propagation through biotissue-mimicking phantoms and real biological tissues. With the help of tissue screening experiments, Monte-Carlo simulation, and the Poincaré sphere approach, we investigated the change of polarimetric properties of light backscattered from the specimens due to the variation in the measurement’s sampling volume, change in tissue scattering and the process of optical clearing.
Conference Chair
Florida International Univ. (United States)
Conference Chair
Tsinghua Univ. Shenzhen International Graduate School (China)
Conference Chair
Lab. de Physique des Interfaces et des Couches Minces (France)
Conference Co-Chair
Imperial College London (United Kingdom)
Conference Co-Chair
Univ. Health Network (Canada)
Program Committee
Institut Fresnel (France)
Program Committee
Christian Brosseau
Univ. de Bretagne Occidentale (France)
Program Committee
Univ. Autònoma de Barcelona (Spain)
Program Committee
Wyant College of Optical Sciences (United States)
Program Committee
Institut Fresnel (France)
Program Committee
Nirmalya Ghosh
Indian Institute of Science Education and Research Kolkata (India)
Program Committee
Univ. of Illinois (United States)
Program Committee
George S. D. Gordon
The Univ. of Nottingham (United Kingdom)
Program Committee
Francois Hache
Lab. d'Optique et Biosciences (France)
Program Committee
Univ. of Washington (United States)
Program Committee
Univ. of Miami (United States)
Program Committee
Yanqiu Li
Nikon Corp. (Japan)
Program Committee
Univ. of Oulu (Finland)
Program Committee
Lab. de Physique des Interfaces et des Couches Minces (France)
Program Committee
Lab. de Physique des Interfaces et des Couches Minces (France)
Program Committee
Saratov State Univ. (Russian Federation)
Program Committee
Univ. of Massachusetts Lowell (United States)