The Moscone Center
San Francisco, California, United States
2 - 7 February 2019
Conference 10882
Multiphoton Microscopy in the Biomedical Sciences XIX
Sunday - Wednesday 3 - 6 February 2019
Conference
Committee
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Conference Chairs
Program Committee
Program Committee continued...
  • Min Gu, RMIT Univ. (Australia)
  • Stefan W. Hell, Max-Planck-Institut für Biophysikalische Chemie (Germany)
  • Fu-Jen Kao, National Yang-Ming Univ. (Taiwan)
  • Arnd K. Krueger, Newport Spectra-Physics GmbH (Germany)
  • Darryl McCoy, Coherent Scotland Ltd. (United Kingdom)
  • Wei Min, Columbia Univ. (United States)
  • Junle Qu, Shenzhen Univ. (China)
  • Angelika C. Rueck, Univ. Ulm (Germany)
  • Yuansheng Sun, ISS, Inc. (United States)
  • Steven S. Vogel, National Institutes of Health (United States)
  • Xiaoliang S. Xie, Harvard Univ. (United States), Peking Univ. (China)
  • Chris Xu, Cornell Univ. (United States)
  • Elena V. Zagaynova, Nizhny Novgorod State Medical Academy (Russian Federation)
  • Bernhard Zimmermann, Carl Zeiss Jena GmbH (Germany)

Sunday 3 February Show All Abstracts
Opening Remarks
Sunday 3 February 2019
8:15 AM - 8:30 AM
Location: Room 160 (South Upper Mezzanine)

Conference Chair: Ammasi Periasamy, Univ. of Virginia (United States)
Session 1:
Keynote Session
Sunday 3 February 2019
8:30 AM - 10:30 AM
Location: Room 160 (South Upper Mezzanine)
Session Chair:
Peter T. C. So, Massachusetts Institute of Technology (United States)
Multi messenger multiphoton microscopy (Keynote Presentation)
Paper 10882-1
Time: 8:30 AM - 9:00 AM
Author(s): Alberto Diaspro, Istituto Italiano di Tecnologia (Italy)
Show Abstract
Highly sensitive chemical microscopy by sensing the thermal effect of infrared absorption (Keynote Presentation)
Paper 10882-2
Time: 9:00 AM - 9:30 AM
Author(s): Ji-Xin Cheng, Boston Univ. (United States)
Show Abstract
Nonlinear endomicroscopy for label-free histological imaging in vivo (Keynote Presentation)
Paper 10882-3
Time: 9:30 AM - 10:00 AM
Author(s): Xingde Li, Johns Hopkins Univ. (United States)
Show Abstract
FLIM, FRET and FLIRR assay for investigating the mitochondrial redox state in cancer cells (Keynote Presentation)
Paper 10882-4
Time: 10:00 AM - 10:30 AM
Author(s): Ammasi Periasamy, Univ. of Virginia (United States)
Show Abstract
Coffee Break 10:30 AM - 11:00 AM
Session 2:
Metabolism/NADH/FAD/Tryptophan I
Sunday 3 February 2019
11:00 AM - 12:15 PM
Location: Room 160 (South Upper Mezzanine)
Session Chair:
Elena V. Zagaynova, Privolzhsky Research Medical Univ. (Russian Federation)
Fast-acquisition TCSPC FLIM with sub-25-ps IRF width (Invited Paper)
Paper 10882-5
Time: 11:00 AM - 11:25 AM
Author(s): Wolfgang Becker, Stefan Smietana, Becker & Hickl GmbH (Germany)
Show Abstract
Multiparametric luminescence lifetime imaging: a new diagnostic tool to follow up bioenergetic alterations during PDT (Invited Paper)
Paper 10882-6
Time: 11:25 AM - 11:50 AM
Author(s): Angelika C. Rück, K. Reeß, Bjorn von Einem, Sviatlana Kalinina, Univ. Ulm (Germany)
Show Abstract
Multicolor two-photon imaging of endogenous fluorophores in living tissues by wavelength mixing (Invited Paper)
Paper 10882-7
Time: 11:50 AM - 12:15 PM
Author(s): Chiara Stringari, Lamiae Abdeladim, Pierre Mahou, Guy Malkinson, Ecole Polytechnique (France); Sébastien Brizion, Jean-Baptiste Galey, L'Oréal Recherche et Innovation (France); Willy Supatto, Ecole Polytechnique (France); Renaud Legouis, Institut de Biologie Intégrative de la Cellule, Univ. Paris-Saclay (France); Ana-Maria Pena, L'Oréal Recherche et Innovation (France); Emmanuel Beaurepaire, Ecole Polytechnique (France)
Show Abstract
Lunch Break 12:15 PM - 1:30 PM
Session 3:
JenLab Young Investigator Award Oral Presentations
Sunday 3 February 2019
1:30 PM - 3:00 PM
Location: Room 160 (South Upper Mezzanine)
Session Chair:
Ammasi Periasamy, Univ. of Virginia (United States)

Candidates include:
  • Spectroscopic stimulated Raman scattering microscopy by ultrafast delay line tuning and deep learning
    Paper 10882-79
    Time: 1:30 PM - 1:35 PM
    Author(s): Haonan Lin, Fengyuan Deng, Chi Zhang, Cheng Zong, Ji-Xin Cheng, Boston Univ. (United States)
  • Hybrid 2-color/3-color background-free broadband CARS with passive polarization optics
    Paper 10882-80
    Time: 1:35 PM - 1:40 PM
    Author(s): Priyank Shah, Brad Littleton, Thomas Kavanagh, David Richards, King's College London (United Kingdom)
  • Pre-resonance stimulated Raman scattering spectroscopy and imaging of membrane potential using near-infrared rhodopsins
    Paper 10882-81
    Time: 1:40 PM - 1:45 PM
    Author(s): Hyeon Jeong Lee, Kai-Chih Huang, Gaoxiang Mei, Natalia Mamaeva, Boston Univ. (United States); Willem J. de Grip, Leiden Univ. (Netherlands); Kenneth J. Rothschild, Ji-Xin Cheng, Boston Univ. (United States)
  • High-speed chemical selective label-free image cytometry for high-content single cell analysis
    Paper 10882-82
    Time: 1:50 PM - 1:55 PM
    Author(s): Kai-Chih Huang, Chi Zhang, Junjie Li, Ji-Xin Cheng, Boston Univ. (United States)
  • Picosecond four wave mixing based fibre amplified diode laser for two photon microscopy (TPM) at 940nm and its application for histology
    Paper 10882-83
    Time: 1:55 PM - 2:00 PM
    Author(s): Jan Philip Kolb, Medizinisches Laserzentrum Lübeck GmbH (Germany); Daniel Weng, Univ. zu Lübeck (Germany); Hubertus Hakert, Medizinisches Laserzentrum Lübeck GmbH (Germany); Matthias Eibl, Univ. zu Lübeck (Germany); Tobias Meyer, Thomas Gottschall, Jürgen Popp, Jens Limpert, Friedrich-Schiller-Univ. Jena (Germany); Sebastian Nino Karpf, Robert Huber, Univ. zu Lübeck (Germany)
  • Simultaneous two-photon and three-photon microscopy imaging with a dual-wavelength Er-doped fiber laser
    Paper 10882-84
    Time: 2:00 PM - 2:05 PM
    Author(s): Lin Huang, Min Ai, Xin Zhou, Shuo Tang, The Univ. of British Columbia (Canada)
  • Assessment of fresh lung tissue using higher harmonic generation microscopy
    Paper 10882-85
    Time: 2:05 PM - 2:10 PM
    Author(s): Laura M. van Huizen, Vrije Univ. Amsterdam (Netherlands); Jouke T. Annema, Pieter Wesseling, Wim-Jan P. van Boven, Amsterdam UMC (Netherlands); Marie Louise Groot, Vrije Univ. Amsterdam (Netherlands)
  • Three-dimensional deep tissue multiphoton frequency-domain fluorescence lifetime imaging microscopy via phase multiplexing and adaptive optics
    Paper 10882-86
    Time: 2:10 PM - 2:15 PM
    Author(s): Yide Zhang, Ian H. Guldner, Evan L. Nichols, David Benirschke, Cody J. Smith, Siyuan Zhang, Scott S. Howard, Univ. of Notre Dame (United States)
  • Comparison of emission wavelengths for in vivo multiphoton imaging of mouse brain
    Paper 10882-87
    Time: 2:15 PM - 2:20 PM
    Author(s): Mengran Wang, Minsu Kim, Chris Xu, Cornell Univ. (United States)
  • Multimodal multiphoton microscopy for visualizing topical drug formulations delivery to human skin
    Paper 10882-88
    Time: 2:20 PM - 2:25 PM
    Author(s): Alexander Fast, Sinyoung Jeong, Sam Osseiran, Massachusetts General Hospital (United States); Maiko Hermsmeier, Tanvee Sawant, Kin F. Chan, BioPharmX, Inc. (United States); Conor L. Evans, Massachusetts General Hospital (United States)
  • Multiphoton microscopy and fluorescence lifetime imaging of the rat and patient liver with cirrhosis ex vivo
    Paper 10882-90
    Time: 2:25 PM - 2:30 PM
    Author(s): Daria Kuznetsova, Varvara V. Dudenkova, Svetlana Rodimova, Privolzhsky Research Medical Univ. (Russian Federation); Nikolai Bobrov, The Volga District Medical Ctr. (Russian Federation); Vladimir Zagainov, The Volga District Medical Ctr. (Russian Federation), Privolzhsky Research Medical Univ. (Russian Federation); Elena V. Zagaynova, Privolzhsky Research Medical Univ. (Russian Federation)
  • Nondestructive method for Chondrocyte Viability Assessment in Articular Cartilage tissues with Nonlinear Optical Microscopy
    Paper 10882-91
    Time: 2:30 PM - 2:35 PM
    Author(s): Yang Li, Clemson Univ. (United States); Elizabeth Nadeau, Russell Reeves, Medical Univ. of South Carolina (United States); Beckham Watkins, Razeen Basunia, College of Charleston (United States); Neal Saini, Medical Univ. of South Carolina (United States); Xun Chen, Clemson Univ. (United States); Vincent Pellegrini, Medical Univ. of South Carolina (United States); Tong Ye, Clemson Univ. (United States)
  • Multimodal automated microscope for wide-scale investigation of protein interactions and genetically encoded biosensors
    Paper 10882-92
    Time: 2:35 PM - 2:40 PM
    Author(s): Tuan A. Nguyen, Henry L. Puhl, An K. Pham, Grace Taumoefolau, Youngchan Kim, Steven S. Vogel, National Institutes of Health (United States)
Coffee Break 3:00 PM - 3:30 PM
Session 4:
Metabolism/NADH/FAD/Tryptophan II
Sunday 3 February 2019
3:30 PM - 4:55 PM
Location: Room 160 (South Upper Mezzanine)
Session Chair:
Angelika C. Rück, Univ. Ulm (Germany)
Interrogation of tumors on macroscale: following metabolic and oxygen status (Invited Paper)
Paper 10882-8
Time: 3:30 PM - 3:55 PM
Author(s): Marina V. Shirmanova, Privolzhsky Research Medical Univ. (Russian Federation); Vladislav I. Shcheslavskiy, Becker & Hickl GmbH (Germany); Anastasia I. Solomatina, Saint Petersburg State Univ. (Russian Federation); Varvara V. Dudenkova, Maria Lukina, Privolzhsky Research Medical Univ. (Russian Federation); Pavel S. Chelushkin, Saint Petersburg State Univ. (Russian Federation); Elena V. Zagaynova, Privolzhsky Research Medical Univ. (Russian Federation); Sergey P. Tunik, Saint Petersburg State Univ. (Russian Federation); Wolfgang Becker, Becker & Hickl GmbH (Germany)
Show Abstract
Multiphoton FLIM imaging of NADH and FAD to analyze cellular metabolic activity of reconstructed human skin in response to UVA light
Paper 10882-9
Time: 3:55 PM - 4:10 PM
Author(s): Ana-Maria Pena, Marine Boulade, Sébastien Brizion, Nicolas Tissot, Thomas Bornschlögl, Jean-Baptiste Galey, Françoise Bernerd, Emilie Planel, L'Oréal Recherche et Innovation (France)
Show Abstract
Metabolic imaging by simultaneous FLIM of NAD(P)H and FAD
Paper 10882-10
Time: 4:10 PM - 4:25 PM
Author(s): Wolfgang Becker, Axel Bergmann, Lukas Z. Braun, Becker & Hickl GmbH (Germany)
Show Abstract
Effects of pH on FAD autofluorescence lifetime
Paper 10882-11
Time: 4:25 PM - 4:40 PM
Author(s): Rebecca Schmitz, Univ. of Wisconsin System (United States); Christine Walsh, Morgridge Institute for Research (United States), Univ. at Albany (United States); Alex Walsh, Morgridge Institute for Research (United States); Melissa Skala, Univ. of Wisconsin System (United States), Morgridge Institute for Research (United States)
Show Abstract
Fluorescence lifetime imaging microscopy (FLIM) resolves the distinct metabolic status of adipose tissues in mice fed on normolipidic diets based on different fatty acids
Paper 10882-12
Time: 4:40 PM - 4:55 PM
Author(s): Danilo Ferrucci, Mariana O. Baratti, Vitor B. Pelegati, Carlos L. Cesar, Hernandes F. Carvalho, Univ. Estadual de Campinas (Brazil)
Show Abstract
Session PSun:
Posters-Sunday
Sunday 3 February 2019
5:30 PM - 7:00 PM
Location: InterContinental Hotel, Grand Ballroom (3rd Floor) & InterContinental Ballroom (5th Floor)
Session Chairs:
Holly Aaron, Univ. of California, Berkeley (United States) ;
Michael Börsch, Universitätsklinikum Jena (Germany) ;
Alberto Diaspro, Istituto Italiano di Tecnologia (Italy) ;
Kevin W. Eliceiri, Univ. of Wisconsin-Madison (United States) ;
Lingyan Shi, Columbia Univ. (United States)

Conference attendees are invited to attend the BiOS poster session on Sunday evening. Come view the posters, enjoy light refreshments, ask questions, and network with colleagues in your field.

Poster Setup: Sunday 10:00 AM – 4:30 PM
View poster presentation guidelines and set-up instructions at
http://spie.org/PWPosterGuidelines
Spectroscopic stimulated Raman scattering microscopy by ultrafast delay line tuning and deep learning
Paper 10882-79
Time: 5:30 PM - 7:00 PM
Author(s): Haonan Lin, Fengyuan Deng, Chi Zhang, Cheng Zong, Ji-Xin Cheng, Boston Univ. (United States)
Show Abstract
Hybrid 2-color/3-color background-free broadband CARS with passive polarization optics
Paper 10882-80
Time: 5:30 PM - 7:00 PM
Author(s): Priyank Shah, Brad Littleton, Thomas Kavanagh, Yu Nie, Stephen Sturzenbaum, David Richards, King's College London (United Kingdom)
Show Abstract
Pre-resonance stimulated Raman scattering spectroscopy and imaging of membrane potential using near-infrared rhodopsins
Paper 10882-81
Time: 5:30 PM - 7:00 PM
Author(s): Hyeon Jeong Lee, Kai-Chih Huang, Gaoxiang Mei, Natalia Mamaeva, Boston Univ. (United States); Willem J. de Grip, Leiden Univ. (Netherlands); Kenneth J. Rothschild, Ji-Xin Cheng, Boston Univ. (United States)
Show Abstract
High-speed chemical selective label-free image cytometry for high-content single cell analysis
Paper 10882-82
Time: 5:30 PM - 7:00 PM
Author(s): Kai-Chih Huang, Chi Zhang, Junjie Li, Ji-Xin Cheng, Boston Univ. (United States)
Show Abstract
Virtual HE histology by fiber-based picosecond two-photon microscopy
Paper 10882-83
Time: 5:30 PM - 7:00 PM
Author(s): Jan Philip Kolb, Medizinisches Laserzentrum Lübeck GmbH (Germany); Daniel Weng, Univ. zu Lübeck (Germany); Hubertus Hakert, Medizinisches Laserzentrum Lübeck GmbH (Germany); Matthias Eibl, Wolfgang Draxinger, Univ. zu Lübeck (Germany); Tobias Meyer, Thomas Gottschall, Friedrich-Schiller-Univ. Jena (Germany); Ralf Brinkmann, Medizinisches Laserzentrum Lübeck GmbH (Germany); Reginald Birngruber, Univ. zu Lübeck (Germany); Jürgen Popp, Jens Limpert, Friedrich-Schiller-Univ. Jena (Germany); Sebastian Nino Karpf, Robert Huber, Univ. zu Lübeck (Germany)
Show Abstract
Simultaneous two-photon and three-photon microscopy imaging with a dual-wavelength Er-doped fiber laser
Paper 10882-84
Time: 5:30 PM - 7:00 PM
Author(s): Lin Huang, Shuo Tang, The Univ. of British Columbia (Canada)
Show Abstract
Assessment of fresh mouse lung tissue using higher harmonic generation microscopy
Paper 10882-85
Time: 5:30 PM - 7:00 PM
Author(s): Laura M. van Huizen, Vrije Univ. Amsterdam (Netherlands); Jouke T. Annema, Pieter Wesseling, Wim-Jan P. van Boven, Amsterdam UMC (Netherlands); Marie Louise Groot, Vrije Univ. Amsterdam (Netherlands)
Show Abstract
Three-dimensional deep tissue multiphoton frequency-domain fluorescence lifetime imaging microscopy via phase multiplexing and adaptive optics
Paper 10882-86
Time: 5:30 PM - 7:00 PM
Author(s): Yide Zhang, Ian H. Guldner, Evan L. Nichols, David Benirschke, Cody J. Smith, Siyuan Zhang, Scott S. Howard, Univ. of Notre Dame (United States)
Show Abstract
Comparison of emission wavelengths for in vivo deep imaging of mouse brain
Paper 10882-87
Time: 5:30 PM - 7:00 PM
Author(s): Mengran Wang, Minsu Kim, Fei Xia, Chris Xu, Cornell Univ. (United States)
Show Abstract
Multimodal multiphoton microscopy for visualizing topical drug formulations delivery to human skin
Paper 10882-88
Time: 5:30 PM - 7:00 PM
Author(s): Alexander Fast, Sinyoung Jeong, Sam Osseiran, Massachusetts General Hospital (United States); Maiko Hermsmeier, Tanvee Sawant, Kin F. Chan, BioPharmX, Inc. (United States); Conor L. Evans, Massachusetts General Hospital (United States)
Show Abstract
Multiphoton microscopy and fluorescence lifetime imaging of the rat and patient liver with cirrhosis ex vivo
Paper 10882-90
Time: 5:30 PM - 7:00 PM
Author(s): Daria Kuznetsova, Svetlana Rodimova, Varvara V. Dudenkova, Privolzhsky Research Medical Univ. (Russian Federation); Nikolai Bobrov, Nikolai Kiselev, The Volga District Medical Ctr. (Russian Federation); Vladimir Zagainov, The Volga District Medical Ctr. (Russian Federation), Privolzhsky Research Medical Univ. (Russian Federation); Elena V. Zagaynova, Privolzhsky Research Medical Univ. (Russian Federation)
Show Abstract
Nondestructive method for chondrocyte viability assessment in articular cartilage tissues with nonlinear optical microscopy
Paper 10882-91
Time: 5:30 PM - 7:00 PM
Author(s): Yang Li, Clemson Univ. (United States); Beckham Watkins, College of Charleston (United States); Elizabeth Nadeau, Russell Reeves, Medical Univ. of South Carolina (United States); Razeen Basunia, Clemson Univ. (United States); Neal Saini, Medical Univ. of South Carolina (United States); Xun Chen, Steven Gannon, Clemson Univ. (United States); Vincent Pellegrini, Medical Univ. of South Carolina (United States); Hai Yao, Tong Ye, Clemson Univ. (United States)
Show Abstract
Multimodal automated microscope for wide-scale investigation of protein interactions and genetically encoded biosensors
Paper 10882-92
Time: 5:30 PM - 7:00 PM
Author(s): Tuan A. Nguyen, Henry L. Puhl, An K. Pham, Grace Taumoefolau, Youngchan Kim, Steven S. Vogel, National Institutes of Health (United States)
Show Abstract
Quantitative multimodal imaging of neurovascular coupling
Paper 10882-94
Time: 5:30 PM - 7:00 PM
Author(s): Andrew Francis, Univ. of Washington (United States)
Show Abstract
CARS spectroscopy of L-threonine crystal by a 30fs chirped pulse
Paper 10882-96
Time: 5:30 PM - 7:00 PM
Author(s): Vitor B. Pelegati, Univ. Estadual de Campinas (Brazil); Manoel L. Alves-Neto, Afonso L. D. Moura, Francisco E. A. Melo, Univ. Federal do Ceará (Brazil); Lazaro A. Padilha, Univ. Estadual de Campinas (Brazil); Carlos L. Cesar, Univ. Federal do Ceará (Brazil), Univ. Estadual de Campinas (Brazil)
Show Abstract
Single-objective multiphoton light-sheet microscopy for tumor organoid screening
Paper 10882-97
Time: 5:30 PM - 7:00 PM
Author(s): Trung D. Nguyen, Yen-Liang Liu, Sarah Bi, Shao-Po Huang, Eric D. Borrego, Yuan-I Chen, Yu-An Kuo, Tim Yeh, The Univ. of Texas at Austin (United States)
Show Abstract
Third order dispersion effects on spectral focusing in coherent Raman microscopy
Paper 10882-98
Time: 5:30 PM - 7:00 PM
Author(s): Mojtaba Mohseni, Christoph Polzer, Thomas Hellerer, Hochschule für Angewandte Wissenschaften München (Germany)
Show Abstract
Two-photon microscopic imaging of capillary red blood cell flux in mouse brain reveals vulnerability of cerebral white matter to hypoperfusion
Paper 10882-99
Time: 5:30 PM - 7:00 PM
Author(s): Baoqiang Li, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States); Ryo Ohtomo, Massachusetts General Hospital (United States); Martin Thunemann, Stephen Adams, Univ. of California, San Diego (United States); Jing Yang, Buyin Fu, Chongzhao Ran, Jonathan Polimeni, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States); David Boas, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States), Boston Univ. (United States); Anna Devor, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States), Univ. of California, San Diego (United States); Eng Lo, Ken Arai, Massachusetts General Hospital (United States); Sava Sakadzic, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States)
Show Abstract
Nanometer-scale colocalization microscopy of Streptococcus pneumoniae filaments
Paper 10882-100
Time: 5:30 PM - 7:00 PM
Author(s): Christoph Polzer, Stefan Ness, Mojtaba Mohseni, Hochschule für Angewandte Wissenschaften München (Germany); Joachim O. Rädler, Ludwig-Maximilians-Universität München (Germany); Markus Hilleringmann, Thomas Hellerer, Hochschule für Angewandte Wissenschaften München (Germany)
Show Abstract
Compact and flexible multiphoton microscope with femtosecond pulse fiber delivery
Paper 10882-101
Time: 5:30 PM - 7:00 PM
Author(s): Hans Georg Breunig, Karsten König, Univ. des Saarlandes (Germany), JenLab GmbH (Germany)
Show Abstract
Novel concept for delay-free tuning of optical parametric oscillators for coherent Raman scattering microscopy
Paper 10882-102
Time: 5:30 PM - 7:00 PM
Author(s): Tim Hellwig, Maximilian Brinkmann, Carsten Fallnich, Westfälische Wilhelms-Univ. Münster (Germany)
Show Abstract
Polarization-resolved SHG microscopy based on continuous rotation of linear polarization with an electro-optic modulator
Paper 10882-103
Time: 5:30 PM - 7:00 PM
Author(s): Takuya Sakaue, Tokushima Univ. (Japan); Eiji Hase, Japan Synchrotron Radiation Research Institute (JASRI) (Japan), Tokushima Univ. (Japan); Takeo Minamikawa, Takeshi Yasui, Tokushima Univ. (Japan)
Show Abstract
Spectrally tunable non-descanned detection: a novel approach for multiphoton microscopy
Paper 10882-104
Time: 5:30 PM - 7:00 PM
Author(s): Giulia Ossato, Susanne Holzmeister, Leica Microsystems GmbH (Germany)
Show Abstract
Fluorescence recovery after photobleaching (FRAP) with simultaneous fluorescence lifetime (FLIM) and time-resolved fluorescence anisotropy imaging (tr-FAIM)
Paper 10882-105
Time: 5:30 PM - 7:00 PM
Author(s): Klaus Suhling, King's College London (United Kingdom)
Show Abstract
Measurement of red blood cell velocity with laser scanning microscopy
Paper 10882-106
Time: 5:30 PM - 7:00 PM
Author(s): Emmanuelle Chaigneau, Serge Charpak, Institut National de la Santé et de la Recherche Médicale (France)
Show Abstract
"Green gap" challenge with LEDs for multiphoton microscopy systems and medical devices
Paper 10882-107
Time: 5:30 PM - 7:00 PM
Author(s): Kavita Aswani, Excelitas Technologies Inc. (Canada)
Show Abstract
pbICS microscopy technique for determining oligomeric state
Paper 10882-108
Time: 5:30 PM - 7:00 PM
Author(s): Alireza Lajevardipour, Andrew Clayton, Swinburne Univ. of Technology (Australia)
Show Abstract
Development of an electrothermal MEMS mirror based two-photon microscopy probe
Paper 10882-109
Time: 5:30 PM - 7:00 PM
Author(s): Liang Zhou, Univ. of Florida (United States); Yanping Chen, Xiaohong Chen, Xiamen Univ. (China), Univ. of Florida (United States); Yunqi Hao, Zhengzhou Univ. of Light Industry (China), Univ. of Florida (United States); Jason Coleman, Huikai Xie, Univ. of Florida (United States)
Show Abstract
Towards field-resolved overtone spectro-microscopy
Paper 10882-110
Time: 5:30 PM - 7:00 PM
Author(s): Ayman Alismail, Haochuan Wang, Ferenc Krausz, Hanieh Fattahi, Max-Planck-Institut für Quantenoptik (Germany)
Show Abstract
Correlated simultaneous fluorescence and phosphorescence lifetime imaging for metabolic mapping and oxygen sensing in living cells
Paper 10882-111
Time: 5:30 PM - 7:00 PM
Author(s): Sviatlana Kalinina, Univ. Ulm (Germany); Patrick Schaefer, Ctr. for Mitochondrial and Epigenomic Medicine, The Children's Hospital of Philadelphia (United States); Bjorn von Einem, Angelika C. Rück, Univ. Ulm (Germany)
Show Abstract
Two-photon luminescence of ultra-small 5 nm gold nanoparticles and its implication in biomedical applications
Paper 10882-112
Time: 5:30 PM - 7:00 PM
Author(s): Sangheon Han, Rice Univ. (United States); Tomasz Zal, Konstantin Sokolov, The Univ. of Texas M. D. Anderson Cancer Ctr. (United States)
Show Abstract
VistaVision toolbox for quantitative multi-parameter analysis of single molecule dynamics
Paper 10882-113
Time: 5:30 PM - 7:00 PM
Author(s): Yuansheng Sun, Sunil Shah, Shih-Chu Jeff Liao, Ulas C. Coskun, Beniamino Barbieri, ISS, Inc. (United States)
Show Abstract
Differentiation of collagens I, II, and III by second order susceptibility imaging
Paper 10882-114
Time: 5:30 PM - 7:00 PM
Author(s): Sheng-Lin Lee, Chiu-Mei Hsueh, Te-Yu Tseng, Ya-Der Huang, Hsuan-Shu Lee, Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
Show Abstract
Label-free imaging of hemoglobin degradation and hemosiderin formation with femtosecond pump-probe microscopy
Paper 10882-115
Time: 5:30 PM - 7:00 PM
Author(s): Minbiao Ji, Fudan Univ. (China)
Show Abstract
FLIM imaging of auto-fluorescent NAD(P)H and FAD to track metabolic changes of non-adherent leukemia cells using microfluidic trapping array
Paper 10882-116
Time: 5:30 PM - 7:00 PM
Author(s): Ruofan Cao, Horst K. Wallrabe, Chunbo Huang, Su-Fern Tan, Yuan Xing, Yong Wang, Jose Oberholzer, Mark Kester, Thomas Loughran, Ammasi Periasamy, Univ. of Virginia (United States)
Show Abstract
Global analysis and Decay Associated Images (DAI) derived from Fluorescence Lifetime Imaging Microscopy (FLIM)
Paper 10882-23
Time: 5:30 PM - 7:00 PM
Author(s): Mitchell Harling, Gregory R. Alspaugh, Alessio Andreoni, National Heart, Lung, and Blood Institute, National Institutes of Health (United States); Aleksander V. Smirnov, National Eye Institute, National Institutes of Health (United States); Rozhin Penjweini, Michael Murphy, Marie-Paule Strub, Jay R. Knutson, National Heart, Lung, and Blood Institute, National Institutes of Health (United States)
Show Abstract
Monday 4 February Show All Abstracts
Session 5:
FLIM/FRET/FCS I
Monday 4 February 2019
8:00 AM - 9:30 AM
Location: Room 160 (South Upper Mezzanine)
Session Chair:
Steven S. Vogel, National Institutes of Health (United States)
Autofluorescence lifetime endoscopy for automated label-free early detection of oral epithelial cancer and dysplasia: a cross-validation in vivo pilot study (Invited Paper)
Paper 10882-13
Time: 8:00 AM - 8:25 AM
Author(s): Javier A. Jo, Texas A&M Univ. (United States); Rodrigo Cuenca, Texas A&M Univ. at Qatar (Qatar); Elvis Duran, Shuna Cheng, Bilal Malik, Texas A&M Univ. (United States); Beena Ahmed, Jim Ji, Texas A&M Univ. (Qatar); Kristen Maitland, Texas A&M Univ. (United States); Yi-Shing Lisa Cheng, John Wright, Texas A&M Health Science Ctr. (United States)
Show Abstract
Subsurface luminescence lifetime imaging of photovoltaic materials using multiphoton tomography (Invited Paper)
Paper 10882-14
Time: 8:25 AM - 8:50 AM
Author(s): Karsten König, Univ. des Saarlandes (Germany), JenLab GmbH (Germany); Benedikt Ursprung, Lawrence Berkeley National Lab. (United States); Hans Georg Breunig, Univ. des Saarlandes (Germany), JenLab GmbH (Germany)
Show Abstract
Multiphoton imaging and OCT MA for diagnosis of human melanocytic lesions (Invited Paper)
Paper 10882-15
Time: 8:50 AM - 9:15 AM
Author(s): Elena V. Zagaynova, Vadim Elagin, Ekatarina Gubarkova, Oksana Garanina, Natalia Orlinskaya, Varvara V. Dudenkova, Irena Shlivko, Irina Klemenova, Diana Davydova, Privolzhsky Research Medical Univ. (Russian Federation)
Show Abstract
Detecting conformational changes of membrane receptors using single-molecule fluorescence anisotropy as FRET read-out
Paper 10882-16
Time: 9:15 AM - 9:30 AM
Author(s): Michael Börsch, Thomas Heitkamp, Lukas Spantzel, Universitätsklinikum Jena (Germany); Reinhard Grisshammer, National Cancer Institute, National Institutes of Health (United States)
Show Abstract
Session 6:
JenLab Young Investigator Award Finalists' Presentations
Monday 4 February 2019
9:30 AM - 10:00 AM
Location: Room 160 (South Upper Mezzanine)
Session Chair:
Ammasi Periasamy, Univ. of Virginia (United States)
Coffee Break 10:00 AM - 10:30 AM
Session 7:
FLIM/FRET/FCS II
Monday 4 February 2019
10:30 AM - 12:15 PM
Location: Room 160 (South Upper Mezzanine)
Session Chair:
Margarida Barroso, Albany Medical College (United States)
The SPLIT approach for enhancing the spatial resolution in pulsed STED microscopy with FastFLIM and phasor plots (Invited Paper)
Paper 10882-17
Time: 10:30 AM - 10:55 AM
Author(s): Giorgio Tortarolo, Istituto Italiano di Tecnologia (Italy), Univ. degli Studi di Genova (Italy); Yuansheng Sun, ISS, Inc. (United States); Sunil Shah, ISS (United States); Beniamino Barbieri, ISS, Inc. (United States); Kai-Wen Teng, Yuji Ishitsuka, Paul R. Selvin, Univ. of Illinois (United States); Luca L. Lanzanò, Istituto Italiano di Tecnologia (Italy); Alberto Diaspro, Istituto Italiano di Tecnologia (Italy), Univ. degli Studi di Genova (Italy); Giuseppe Vicidomini, Istituto Italiano di Tecnologia (Italy)
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Functional in vivo imaging using fluorescence lifetime light sheet microscopy (Invited Paper)
Paper 10882-18
Time: 10:55 AM - 11:20 AM
Author(s): Simon M. Ameer-Beg, Claire A. Mitchell, Simon P. Poland, Robert D. Knight, Guoqing Wang, King's College London (United Kingdom); Gerhard Holst, PCO AG (Germany)
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Quantitative ultra-fast FLIM and lifetime based multi-species analysis (Invited Paper)
Paper 10882-19
Time: 11:20 AM - 11:45 AM
Author(s): Benedikt Kraemer, Sumeet Rohilla, Rhys Dowler, Marcus Sackrow, Matthias Patting, Hans-Jürgen Rahn, Michael Wahl, Uwe Ortmann, Felix Koberling, PicoQuant GmbH (Germany); Andreas Hocke, Charité Universitätsmedizin Berlin (Germany); Rainer Erdmann, PicoQuant GmbH (Germany)
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Skin research with multiphoton FLIM
Paper 10882-20
Time: 11:45 AM - 12:00 PM
Author(s): Hauke Studier, Wolfgang Becker, Becker & Hickl GmbH (Germany)
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Phasor-based widefield FLIM using a gated 512×512 single-photon SPAD imager
Paper 10882-21
Time: 12:00 PM - 12:15 PM
Author(s): Arin Can Ulku, Claudio Bruschini, Ivan Michel Antolovic, Ecole Polytechnique Fédérale de Lausanne (Switzerland); Yung Kuo, Rinat Ankri, Shimon Weiss, Xavier Michalet, Univ. of California, Los Angeles (United States); Edoardo Charbon, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
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Lunch Break 12:15 PM - 1:30 PM
Session 8:
FLIM/FRET/FCS III
Monday 4 February 2019
1:30 PM - 2:45 PM
Location: Room 160 (South Upper Mezzanine)
Session Chair:
Beniamino B. Barbieri, ISS, Inc. (United States)
IRDye QC-1 as a near-infrared dark acceptor for fluorescence lifetime FRET measurements in cells and in live intact animals (Invited Paper)
Paper 10882-22
Time: 1:30 PM - 1:55 PM
Author(s): Sez-Jade K. Chen, Rensselaer Polytechnic Institute (United States); Alena Rudkouskaya, Joseph Mazurkiewicz, Albany Medical College (United States); Marien Ochoa Mendoza, Nattawut Sinsuebphon, Rensselaer Polytechnic Institute (United States); Margarida Barroso, Albany Medical College (United States); Xavier Intes, Rensselaer Polytechnic Institute (United States)
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Genetically encoded FRET probes for direct mapping and quantification of intracellular oxygenation level via fluorescence lifetime imaging (Invited Paper)
Paper 10882-93
Time: 1:55 PM - 2:20 PM
Author(s): Alessio Andreoni, Rozhin Penjweini, Branden W. Roarke, Marie-Paule Strub, Dan L. Sackett, Jay R. Knutson, National Institutes of Health (United States)
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AB/FCS-fingerprinting: an optical technique for characterizing fluorophore independence in solution (Invited Paper)
Paper 10882-24
Time: 2:20 PM - 2:45 PM
Author(s): Youngchan Kim, Henry L. Puhl, Grace Taumoefolau, Tuan A. Nguyen, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health (United States); Paul S. Blank, National Institute of Child Health and Human Development, National Institutes of Health (United States); Steven S. Vogel, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health (United States)
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Award Presentation
Monday 4 February 2019
2:45 PM - 3:05 PM
Location: Room 160 (South Upper Mezzanine)
Session Chair:
Ammasi Periasamy, Univ. of Virginia (United States)
Coffee Break 3:05 PM - 3:30 PM
Session 9:
Technology Development I
Monday 4 February 2019
3:30 PM - 5:00 PM
Location: Room 160 (South Upper Mezzanine)
Session Chair:
Kevin W. Eliceiri, Univ. of Wisconsin-Madison (United States)
Advances in ultrafast laser technology for faster imaging and manipulation in biological imaging
Paper 10882-25
Time: 3:30 PM - 3:45 PM
Author(s): Sebastian Peck, Spectra-Physics, a division of MKS Instruments (United States)
Show Abstract
Recent advances in femtosecond lasers for non-linear functional imaging of the brain and other tissues
Paper 10882-26
Time: 3:45 PM - 4:00 PM
Author(s): Marco Arrigoni, Darryl McCoy, Coherent, Inc. (United States)
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Freely tunable spectral detection for multi-photon microscopy
Paper 10882-27
Time: 4:00 PM - 4:15 PM
Author(s): Hilmar Gugel, Ingo Boehm, Felix Neugart, Leica Microsystems CMS GmbH (Germany)
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Low-cost fabrication of a high-performance femtosecond fiber laser for multiphoton microscopy
Paper 10882-28
Time: 4:15 PM - 4:30 PM
Author(s): Nima Davoudzadeh, Kai Zhang, Guilluame Ducourthial, Bryan Q. Spring, Northeastern Univ. (United States)
Show Abstract
920 nm fiber laser delivering 100 fs pulses for nonlinear microscopy
Paper 10882-29
Time: 4:30 PM - 4:45 PM
Author(s): Thomas Hellerer, Christoph Polzer, Hochschule für Angewandte Wissenschaften München (Germany); Axel Friedenauer, Isabel Greisberger, Bernhard Wolfring, Christoph Skrobol, TOPTICA Photonics AG (Germany)
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Ultra-broad tunable low noise multi-color light source for multi-photon imaging and spectroscopy
Paper 10882-30
Time: 4:45 PM - 5:00 PM
Author(s): Heiko Linnenbank, Stuttgart Instruments GmbH (Germany), Univ. Stuttgart (Germany); Tobias Steinle, Univ. Stuttgart (Germany); Florian Mörz, Stuttgart Instruments GmbH (Germany), Univ. Stuttgart (Germany); Moritz Floess, Univ. Stuttgart (Germany); Francisco Robles, Georgia Institute of Technology & Emory Univ. School of Medicine (United States); Stefan Kedenburg, Univ. Stuttgart (Germany); Harald Giessen, Stuttgart Instruments GmbH (Germany), Univ. Stuttgart (Germany)
Show Abstract
Tuesday 5 February Show All Abstracts
Session 10:
FLIM/FRET/FCS IV
Tuesday 5 February 2019
8:00 AM - 9:10 AM
Location: Room 160 (South Upper Mezzanine)
Session Chair:
Wolfgang Becker, Becker & Hickl GmbH (Germany)
Ligand-induced stepwise oligomerization of the human GPCR neurotensin receptor 1 in living cells monitored by FLIM, homoFRET and SIM (Invited Paper)
Paper 10882-31
Time: 8:00 AM - 8:25 AM
Author(s): Michael Börsch, Anika Westphal, Stefanie Reuter, Hendrik Sielaff, Ralf Mrowka, Universitätsklinikum Jena (Germany)
Show Abstract
Ophthalmic FLIM for eye disease studies: advanced technical solutions
Paper 10882-32
Time: 8:25 AM - 8:40 AM
Author(s): Cornelia Junghans, Hauke Studier, Wolfgang Becker, Becker & Hickl GmbH (Germany)
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Fluorescence lifetime imaging with single-photon SPAD arrays using long overlapping gates: an experimental and theoretical study
Paper 10882-33
Time: 8:40 AM - 8:55 AM
Author(s): Andrei Ardelean, Arin C. Ulku, Ecole Polytechnique Fédérale de Lausanne (Switzerland); Xavier Michalet, Univ. of California, Los Angeles (United States); Edoardo Charbon, Claudio Bruschini, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Show Abstract
Two-photon photoluminescence excitation and fluorescence lifetime spectroscopy for oil characterization
Paper 10882-34
Time: 8:55 AM - 9:10 AM
Author(s): Manoel L. Alves-Neto, Afonso L. D. Moura, Rosemayre Souza Freire, Francisco C. C. S. Salomão, Francisco Nepomuceno Filho, Univ. Federal do Ceará (Brazil); Hernandes F. Carvalho, Univ. Estadual de Campinas (Brazil); Vitor B. Pelegati, Instituto de Física "Gleb Wataghin", Univ. Estadual de Campinas (Brazil); Carlos L. Cesar, Univ. Federal do Ceará (Brazil), Instituto de Física "Gleb Wataghin", Univ. Estadual de Campinas (Brazil)
Show Abstract
Session 11:
Second/Third Harmonic Generation Microscopy I
Tuesday 5 February 2019
9:10 AM - 10:05 AM
Location: Room 160 (South Upper Mezzanine)
Session Chair:
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
Analysis of collagen architecture alterations in human ovarian cancer and idiopathic pulmonary fibrosis via SHG pixel based polarization analyses (Invited Paper)
Paper 10882-35
Time: 9:10 AM - 9:35 AM
Author(s): Paul J. Campagnola, Kirby Campbell, Darian James, Rajeev Chaudhary, Julia Handel, Univ. of Wisconsin-Madison (United States)
Show Abstract
Fast Fourier transform second harmonic generation microscopy for probing the three-dimensional structure of corneal lamella
Paper 10882-36
Time: 9:35 AM - 9:50 AM
Author(s): Sheng-Lin Lee, Yang-Fang Chen, Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
Show Abstract
Cardiac applications of second harmonic generation (SHG) microscopy
Paper 10882-37
Time: 9:50 AM - 10:05 AM
Author(s): Che-Wei Chang, UC Davis Medical Ctr. (United States); Hannah Ledford, Univ. of California, Davis (United States); Hillary Kao, UC Davis Medical Ctr. (United States); Xiaodong Zhang, Nipavan Chiamvimonvat, Univ. of California, Davis (United States); Deborah K. Lieu, James W. Chan, UC Davis Medical Ctr. (United States)
Show Abstract
Coffee Break 10:05 AM - 10:35 AM
Session 12:
Second/Third Harmonic Generation Microscopy II
Tuesday 5 February 2019
10:35 AM - 11:50 AM
Location: Room 160 (South Upper Mezzanine)
Session Chair:
Paul J. Campagnola, Univ. of Wisconsin-Madison (United States)
Spectrally resolved multiphoton microscopy for the identification of biomarkers
Paper 10882-38
Time: 10:35 AM - 10:50 AM
Author(s): Björn-Ole Meyer, Dominik Marti, Peter E. Andersen, DTU Fotonik (Denmark)
Show Abstract
Assessing changes of collagen fiber organization and cellular metabolism during the onset of osteoarthritis by non-invasive label-free imaging
Paper 10882-39
Time: 10:50 AM - 11:05 AM
Author(s): Zhiyi Liu, Carrie Hui Mingalone, Yang Zhang, Li Zeng, Irene Georgakoudi, Tufts Univ. (United States)
Show Abstract
Multiphoton induced fluorescence and second harmonic imaging of drug-eluted arterial walls for non-invasive photo-polymerizable stents
Paper 10882-40
Time: 11:05 AM - 11:20 AM
Author(s): Tochukwu Emeakaroha, Divya Kota, South Dakota School of Mines and Technology (United States); Sam Young, Ron Utecht, Alumend, LLC (United States); Steve J. Smith, South Dakota School of Mines and Technology (United States)
Show Abstract
Connecting Mueller and Jones tensors for describing nonlinear optical conversion of unpolarized light
Paper 10882-41
Time: 11:20 AM - 11:35 AM
Author(s): Garth J. Simpson, Purdue Univ. (United States)
Show Abstract
Exploratory investigation of the spatial relationships of collagen and nerves in subcutaneous white adipose tissue (scWAT) using 2-photon microscopy
Paper 10882-42
Time: 11:35 AM - 11:50 AM
Author(s): Patrick Breeding, Univ. of Maine (United States); Magdalena Blaszkiewicz, Kristy Townsend, The Univ. of Maine (United States); Andre Khalil, Karissa Tilbury, Univ. of Maine (United States)
Show Abstract
Lunch Break 11:50 AM - 1:35 PM
Session 13:
In Vivo/Intravital Imaging
Tuesday 5 February 2019
1:35 PM - 3:00 PM
Location: Room 160 (South Upper Mezzanine)
Session Chair:
Vladislav I. Shcheslavskiy, Becker & Hickl GmbH (Germany)
Ultracompact multimodal multiphoton microscope for small animal research (Invited Paper)
Paper 10882-43
Time: 1:35 PM - 2:00 PM
Author(s): Karsten König, Univ. des Saarlandes (Germany), JenLab GmbH (Germany); Ana Batista, Univ. des Saarlandes (Germany); Martin Weinigel, JenLab GmbH (Germany); Aisada König, Univ. des Saarlandes (Germany), JenLab GmbH (Germany)
Show Abstract
In vivo multiphoton kinetic imaging of the toxic effect of carbon tetrachloride (CCl4) on hepatobiliary metabolism
Paper 10882-44
Time: 2:00 PM - 2:15 PM
Author(s): Hsu-Cheng Huang, Sheng-Lin Lee, Hsuan-Shu Lee, Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
Show Abstract
Multi-color fiber-optic two-photon endomicroscopy for neuroimaging
Paper 10882-45
Time: 2:15 PM - 2:30 PM
Author(s): Honghua Guan, Wenxuan Liang, Ang Li, Yung-Tian Gau, Dwight Bergles, Johns Hopkins Univ. (United States); Ming-Jun Li, Corning Incorporated (United States); Xingde Li, Johns Hopkins Univ. (United States)
Show Abstract
Two-photon focal modulation microscopy for deep tissue imaging
Paper 10882-46
Time: 2:30 PM - 2:45 PM
Author(s): Rui Chen, National Univ. of Singapore (Singapore); Younghoon Shin, Singapore-MIT Alliance for Research and Technology (Singapore); E. Du, Shuhao Shen, National Univ. of Singapore (Singapore); Peter T. C. So, Massachusetts Institute of Technology (United States); Nanguang Chen, National Univ. of Singapore (Singapore)
Show Abstract
Multiphoton microscopy for label-free histology of human brain tissues
Paper 10882-47
Time: 2:45 PM - 3:00 PM
Author(s): Defu Chen, David W. Nauen, Johns Hopkins Univ. (United States); Hyeon-Cheol Park, The Johns Hopkins Univ. (United States); Wu Yuan, Ang Li, Honghua Guan, Xingde Li, Johns Hopkins Univ. (United States)
Show Abstract
Coffee Break 3:00 PM - 3:30 PM
Session 14:
Technology Development II
Tuesday 5 February 2019
3:30 PM - 5:25 PM
Location: Room 160 (South Upper Mezzanine)
Session Chair:
Karsten Koenig, JenLab GmbH (Germany)
Scanless volumetric imaging by selective access multifoci multiphoton microscopy (Invited Paper)
Paper 10882-48
Time: 3:30 PM - 3:55 PM
Author(s): Yi Xue, Kalen Paul Berry, Christopher James Rowlands, Massachusetts Institute of Technology (United States); Yu Takiguchi, Hamamatsu Photonics K.K. (Japan); Elly Nedivi, Peter T. C. So, Massachusetts Institute of Technology (United States)
Show Abstract
Wide-field multiphoton imaging in scattering media (TRAFIX)
Paper 10882-49
Time: 3:55 PM - 4:10 PM
Author(s): Adrià Escobet-Montalbán, Kishan Dholakia, Mingzhou Chen, Univ. of St. Andrews (United Kingdom); Roman Spesyvtsev, Univ. of Strathclyde (United Kingdom); Wardiya Afshar Saber, Univ. of St. Andrews (United Kingdom); Melissa R. Andrews, Univ. of Southampton (United Kingdom); Michael Mazilu, Univ. of St. Andrews (United Kingdom); C. Simon Herrington, The Univ. of Edinburgh (United Kingdom)
Show Abstract
DMD-based two-photon microscope for fast 3D imaging and optogenetics applications
Paper 10882-50
Time: 4:10 PM - 4:25 PM
Author(s): Shih-Chi Chen, Qiang Geng, The Chinese Univ. of Hong Kong (Hong Kong, China)
Show Abstract
Rapid evaluation of prostate tissue surgical specimens using nonlinear microscopy
Paper 10882-51
Time: 4:25 PM - 4:40 PM
Author(s): Lucas C. Cahill, Tadayuki Yoshitake, Michael G. Giacomelli, Massachusetts Institute of Technology (United States); Yubo Wu, Douglas I. Lin, Huihui Ye, Beth Israel Deaconess Medical Ctr. (United States); Oscar M. Carrasco-Zevallos, Massachusetts Institute of Technology (United States); Andrew A. Wagner, Seymour Rosen, Beth Israel Deaconess Medical Ctr. (United States); James G. Fujimoto, Massachusetts Institute of Technology (United States)
Show Abstract
GaAsP hybrid detectors in the analog mean-delay fluorescence lifetime imaging microscopy
Paper 10882-52
Time: 4:40 PM - 4:55 PM
Author(s): Dongeun Kim, Wonsang Hwang, Dug Young Kim, Yonsei Univ. (Korea, Republic of); Youngjae Won, Medical Device Development Ctr., Osong Medical Innovation Foundation (Korea, Republic of); Sucbei Moon, Kookmin Univ. (Korea, Republic of); Sang Yoon Lee, Min Gu Kang, Won Sub Han, INTEKPLUS Co., Ltd. (Korea, Republic of)
Show Abstract
Transient absorption imaging of cytochrome using pump-probe microscopy
Paper 10882-53
Time: 4:55 PM - 5:10 PM
Author(s): Erkang Wang, Randy A. Bartels, Adam J. Chicco, Jesse W. Wilson, Colorado State Univ. (United States)
Show Abstract
The features of temporal focusing multiphoton excitation fluorescence microscopy using the different excitation wavelength
Paper 10882-54
Time: 5:10 PM - 5:25 PM
Author(s): Fan-Ching Chien, National Central Univ. (Taiwan); Chun-Yu Lin, Chia-Yuan Chang, National Cheng Kung Univ. (Taiwan); Chi-Hsiang Lien, National United Univ. (Taiwan)
Show Abstract
Wednesday 6 February Show All Abstracts
Session 15:
Nonlinear and Linear Chemical Imaging I
Wednesday 6 February 2019
8:00 AM - 10:00 AM
Location: Room 160 (South Upper Mezzanine)
Session Chair:
Ji-Xin Cheng, Boston Univ. (United States)
Pump-probe imaging illuminates molecular changes in metastatic melanoma (Invited Paper)
Paper 10882-55
Time: 8:00 AM - 8:25 AM
Author(s): Warren S. Warren, Duke Univ. (United States)
Show Abstract
Label-free spectroscopic imaging unravels altered lipid metabolism in human renal cell cancer (Invited Paper)
Paper 10882-56
Time: 8:25 AM - 8:50 AM
Author(s): Shuhua Yue, Sishan Cui, Shuo Zhang, Beihang Univ. (China); Shuai Yan, Huazhong Univ. of Science and Technology (China); Lin Yao, Yu Fan, Peking Univ. First Hospital (China); Ping Wang, Huazhong Univ. of Science and Technology (China); Liqun Zhou, Peking Univ. First Hospital (China)
Show Abstract
Imaging chemical and physical stability of drug formulations with stimulated Raman scattering microscopy (Invited Paper)
Paper 10882-57
Time: 8:50 AM - 9:15 AM
Author(s): Dan Fu, Univ. of Washington (United States)
Show Abstract
Label-free volumetric quantitative imaging of the human somatic cell division by hyperspectral coherent anti-Stokes Raman scattering
Paper 10882-58
Time: 9:15 AM - 9:30 AM
Author(s): Arnica Karuna, Francesco Masia, Rachel Errington, Marie Wiltshire, Paola Borri, Wolfgang Langbein, Cardiff Univ. (United Kingdom)
Show Abstract
Stimulated Raman scattering endoscopy for in vivo label-free histology
Paper 10882-59
Time: 9:30 AM - 9:45 AM
Author(s): Peng Lin, Shufan Chen, Chien-Sheng Liao, Fengyuan Deng, Boston Univ. (United States); Oliver Joshua Spires, The Univ. of Arizona (United States); Yi Zhang, Boston Univ. (United States); Rongguang Liang, College of Optical Sciences, The Univ. of Arizona (United States); Ji-Xin Cheng, Boston Univ. (United States)
Show Abstract
Label-free imaging of tumorous tissue in the Raman fingerprint region with time-encoded (TICO) stimulated Raman scattering
Paper 10882-60
Time: 9:45 AM - 10:00 AM
Author(s): Hubertus Hakert, Medizinisches Laserzentrum Lübeck GmbH (Germany); Matthias Eibl, Sebastian Nino Karpf, Institut für Biomedizinische Optik, Univ. zu Lübeck (Germany); Barbara Wollenberg, Ralph Pries, Univ. zu Lübeck (Germany); Robert Huber, Institut für Biomedizinische Optik, Univ. zu Lübeck (Germany)
Show Abstract
Coffee Break 10:00 AM - 10:30 AM
Session 16:
Nonlinear and Linear Chemical Imaging II
Wednesday 6 February 2019
10:30 AM - 12:30 PM
Location: Room 160 (South Upper Mezzanine)
Session Chair:
Shuhua Yue, Beihang Univ. (China)
Vibrational imaging with third-order sum-frequency generation microscopy (Invited Paper)
Paper 10882-61
Time: 10:30 AM - 10:55 AM
Author(s): Adam M. Hanninen, Richard C. Prince, Eric O. Potma, Univ. of California, Irvine (United States)
Show Abstract
Vibrational imaging of metabolic dynamics in animals by DO-SRS (Invited Paper)
Paper 10882-62
Time: 10:55 AM - 11:20 AM
Author(s): Wei Min, Lingyan Shi, Columbia Univ. (United States)
Show Abstract
Intelligent image-activated cell sorting: principles and applications (Invited Paper)
Paper 10882-63
Time: 11:20 AM - 11:45 AM
Author(s): Keisuke Goda, Nao Nitta, Takeaki Sugimura, The Univ. of Tokyo (Japan); Yoichiroh Hosokawa, Nara Institute of Science and Technology (Japan); Sotaro Uemura, Yasuyuki Ozeki, The Univ. of Tokyo (Japan)
Show Abstract
Bond-selective phase contrast microscopy
Paper 10882-64
Time: 11:45 AM - 12:00 PM
Author(s): Lu Lan, Delong Zhang, Boston Univ. (United States); Yeran Bai, National Lab. on High Power Laser and Physics (China); Hassaan Majeed, Mikhail E. Kandel, Gabriel Popescu, Univ. of Illinois (United States); Ji-Xin Cheng, Boston Univ. (United States)
Show Abstract
Nonlinear Raman and second harmonic imaging with quantum light
Paper 10882-65
Time: 12:00 PM - 12:15 PM
Author(s): Vladislav V. Yakovlev, Texas A&M Univ. (United States)
Show Abstract
Common-mode balanced detection in stimulated Raman scattering microscopy
Paper 10882-66
Time: 12:15 PM - 12:30 PM
Author(s): Carlo Valensise, Politecnico di Milano (Italy); Vikas Kumar, Univ. Duisburg-Essen (Germany); Francesco Crisafi, Univ. Zürich (Switzerland); Marco Marangoni, Giulio Cerullo, Dario Polli, Politecnico di Milano (Italy)
Show Abstract
Lunch Break 12:30 PM - 1:30 PM
Session 17:
Nonlinear and Linear Chemical Imaging III
Wednesday 6 February 2019
1:30 PM - 3:15 PM
Location: Room 160 (South Upper Mezzanine)
Session Chair:
Wei Min, Columbia Univ. (United States)
Understanding lipid metabolism using stimulated Raman scattering microscopy (Invited Paper)
Paper 10882-67
Time: 1:30 PM - 1:55 PM
Author(s): Meng Wang, Baylor College of Medicine (United States)
Show Abstract
Single unnatural amino acid carried Raman tags for stimulated Raman imaging of specific proteins (Invited Paper)
Paper 10882-68
Time: 1:55 PM - 2:20 PM
Author(s): Jing Zhang, Ping Wang, Huazhong Univ. of Science and Technology (China); Shuhua Yue, Beihang Univ. (China)
Show Abstract
Chemical imaging measurements of biological and 3D printed systems for cancer pathology (Invited Paper)
Paper 10882-69
Time: 2:20 PM - 2:45 PM
Author(s): Rohit Bhargava, Mark Gryka, Shachi Mittal, Kevin Yeh, Sudipta Mukherjee, Beckman Institute for Advanced Science and Technology (United States)
Show Abstract
Hyperspectral stimulated Raman scattering imaging to determine spatially resolved chemical composition of breast calcifications in association with neoplastic processes
Paper 10882-70
Time: 2:45 PM - 3:00 PM
Author(s): Kseniya Shin, Mint Laohajaratsang, Suzanne Dintzis, Dan Fu, Univ. of Washington (United States)
Show Abstract
Nanoscale organization of proteins assemblies probed by polarized label free multiphoton fluorescence
Paper 10882-71
Time: 3:00 PM - 3:15 PM
Author(s): Sophie Brasselet, Institut Fresnel (France); Ellen Green, Peter Winlove, Univ. of Exeter (United Kingdom)
Show Abstract
Coffee Break 3:15 PM - 3:45 PM
Session 18:
Nonlinear and Linear Chemical Imaging IV
Wednesday 6 February 2019
3:45 PM - 5:50 PM
Location: Room 160 (South Upper Mezzanine)
Session Chair:
Eric O. Potma, Univ. of California, Irvine (United States)
Compressive Raman microspectroscopy (Invited Paper)
Paper 10882-72
Time: 3:45 PM - 4:10 PM
Author(s): Hilton B. de Aguiar, École normale supérieure Paris-Saclay (France)
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Slide-free molecular histopathology using simultaneous label-free auto-fluorescence multi-harmonic (SLAM) microscopy (Invited Paper)
Paper 10882-73
Time: 4:10 PM - 4:35 PM
Author(s): Stephen Allen Boppart, Univ. of Illinois (United States)
Show Abstract
Quantitation of glycated hemoglobin in single red blood cells by transient absorption microscopy and phasor analysis
Paper 10882-74
Time: 4:35 PM - 4:50 PM
Author(s): Pu-Ting Dong, Haonan Lin, Ji-Xin Cheng, Boston Univ. (United States)
Show Abstract
Label-free molecular subtyping of glioblastomas with epi-detected hyperspectral stimulated Raman scattering microscopy
Paper 10882-75
Time: 4:50 PM - 5:05 PM
Author(s): Bae Kideogg, Wei Zheng, Kan Lin, Carol Tang, Christopher Beng Ti Ang, Zhiwei Huang, National Univ. of Singapore (Singapore)
Show Abstract
Following the diffusion of cryopreservatives in tissues by stimulated Raman scattering microscopy
Paper 10882-76
Time: 5:05 PM - 5:20 PM
Author(s): Richard C. Prince, Eric O. Potma, Univ. of California, Irvine (United States); Nancy Levinger, Colorado State Univ. (United States)
Show Abstract
Clearing-enhanced volumetric chemical imaging by stimulated Raman scattering microscopy
Paper 10882-77
Time: 5:20 PM - 5:35 PM
Author(s): Lingyan Shi, Mian Wei, Lu Wei, Wei Min, Columbia Univ. (United States)
Show Abstract
3D printed high-NA ultra-thin catadioptric condenser to minimize background contribution from cross-phase modulation in stimulated Raman scattering microscopy
Paper 10882-78
Time: 5:35 PM - 5:50 PM
Author(s): Andrea Bertoncini, King Abdullah Univ. of Science and Technology (Italy); Carlo Liberale, King Abdullah Univ. of Science and Technology (Saudi Arabia)
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