The Moscone Center
San Francisco, California, United States
1 - 6 February 2020
Conference 11244
Multiphoton Microscopy in the Biomedical Sciences XX
Sunday - Tuesday 2 - 4 February 2020
Conference
Committee
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Conference Chairs
Program Committee
  • Holly Aaron, Univ. of California, Berkeley (United States)
  • Margarida Barroso, Albany Medical College (United States)
  • Wolfgang Becker, Becker & Hickl GmbH (Germany)
  • Paul J. Campagnola, Univ. of Wisconsin-Madison (United States)
  • Ji-Xin Cheng, Purdue Univ. (United States)
  • Alberto Diaspro, Istituto Italiano di Tecnologia (Italy)
  • Michelle Digman, Univ. of California, Irvine (United States)
  • Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
  • Kevin W. Eliceiri, Univ. of Wisconsin-Madison (United States)
  • Scott Fraser, The Univ. of Southern California (United States)
  • Katsumasa Fujita, Osaka Univ. (Japan)
  • Enrico Gratton, Univ. of California, Irvine (United States)
  • Min Gu, RMIT Univ. (Australia)
  • Stefan W. Hell, Max-Planck-Institut für Biophysikalische Chemie (Germany)

Program Committee continued...
Additional Conference
Information
In 2020: Celebration of 30 years of Multiphoton Microscopy
Sunday 2 February Show All Abstracts
Opening Remarks
Sunday 2 February 2020
8:00 AM - 8:15 AM
Session Chair:
Ammasi Periasamy, Univ. of Virginia (United States)
Session 1:
Keynote Session
Sunday 2 February 2020
8:15 AM - 9:45 AM
Session Chair:
Ammasi Periasamy, Univ. of Virginia (United States)
Leaving any number of photons behind: Adventures in structural neurobiology (Keynote Presentation)
Paper 11244-1
Author(s): Winfried Denk, Max-Planck-Institut für Neurobiologie (Germany)
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Evolution of multiphoton microscopy over three decades: Current perspectives and future directions (Keynote Presentation)
Paper 11244-2
Author(s): Paras N. Prasad, Univ. at Buffalo (United States)
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Spectral phasors by the sin-cos filter method in a two-photon excitation microscope (Keynote Presentation)
Paper 11244-3
Author(s): Enrico Gratton, Alexander Dvornikov, Univ. of California, Irvine (United States)
Show Abstract
Session 2:
Multiphoton Microscopy and Applications I
Sunday 2 February 2020
9:45 AM - 12:10 PM
Session Chair:
Karsten König, JenLab GmbH (Germany)
Early applications of multiphoton microscopy at Cornell (Invited Paper)
Paper 11244-4
Author(s): Warren R. Zipfel, Cornell Univ. (United States)
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Multicolor spatial cumulant analysis to measure dopamine receptor dynamics on the plasma membrane (Invited Paper)
Paper 11244-5
Author(s): Daniel J. Foust, Allesandro Ustione, David Piston, Washington Univ. in St. Louis (United States)
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Imaging deeper, wider, and faster (Invited Paper)
Paper 11244-6
Author(s): Chris Xu, Cornell Univ. (United States)
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Advances in adaptive optics for multi-photon microscopy (Invited Paper)
Paper 11244-7
Author(s): Martin J. Booth, Univ. of Oxford (United Kingdom)
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High-speed multiphoton microscopy for imaging the brain (Invited Paper)
Paper 11244-8
Author(s): Na Ji, Univ. of California, Berkeley (United States)
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Label-free classification of T cell activation (Invited Paper)
Paper 11244-9
Author(s): Melissa C. Skala, Morgridge Institute for Research (United States), Univ. of Wisconsin-Madison (United States); Alex J. Walsh, Kelsey Tweed, Morgridge Institute for Research (United States); Katie Mueller, Univ. of Wisconsin-Madison (United States); Isabel Jones, Steven M. Trier, Morgridge Institute for Research (United States); Krishanu Saha, Univ. of Wisconsin-Madison (United States)
Show Abstract
Lunch: Celebrating 30 years Multiphoton Microscopy
Sunday 2 February 2020
12:10 PM - 1:20 PM

Authors of conference 11244 are welcome to join us for lunch to Celebrate 30 Years of Multiphoton Microscopy

Registration Badges are required to attend
Session 3:
Multiphoton Microscopy and Applications II
Sunday 2 February 2020
1:20 PM - 3:00 PM
Session Chair:
Peter T. C. So, Massachusetts Institute of Technology (United States)
Translation of two-photon microscopy to the clinic: Multiphoton CARS tomography of in vivo human skin (Invited Paper)
Paper 11244-10
Author(s): Karsten König, Hans Georg Breunig, Univ. des Saarlandes (Germany), JenLab GmbH (Germany); Ana Maria Gonçalves Batista, Univ. des Saarlandes (Germany); Andeas Schindele, JenLab GmbH (Germany); Michael Zieger, Martin Kaatz, SRH Wald-Klinikum Gera GmbH (Germany)
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Multimodal non-linear imaging for tissue diagnosis, therapy and therapy-monitoring (Invited Paper)
Paper 11244-11
Author(s): Jürgen Popp, Leibniz-Institut für Photonische Technologien e.V. (Germany)
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Rapid non-linear image scanning microscopy (Invited Paper)
Paper 11244-12
Author(s): Ingo Gregor, Jörg Enderlein, Georg-August-Univ. Göttingen (Germany); Robert Ros, Arizona State Univ. (United States)
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Two-photon time-resolved anisotropy, FCS, and photon antibunching reveal excitonic coupling between fluorescent proteins. (Invited Paper)
Paper 11244-13
Author(s): Steven S. Vogel, National Institutes of Health (United States)
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Visible-wavelength two-photon excitation for high-resolution microscopy and microfabrication (Invited Paper)
Paper 11244-14
Author(s): Katsumasa Fujita, Osaka Univ. (Japan)
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Session 4:
Multiphoton Microscopy and Applications III
Sunday 2 February 2020
3:30 PM - 5:00 PM
Session Chair:
Angelika C. Rueck, Univ. Ulm (Germany)
New paradigms in femtosecond lasers for non-linear imaging of the brain and other tissues
Paper 11244-15
Author(s): Marco Arrigoni, Coherent, Inc. (United States); Darryl McCoy, Coherent Scotland Ltd. (United Kingdom)
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Developments in ultrafast laser technology enable advancements in in vivo imaging and optogenetics
Paper 11244-16
Author(s): Sebastian Peck, Spectra-Physics, a division of MKS Instruments (United States)
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Free-electron-mediated modifications of biomolecules: from photodamage in nonlinear microscopy to intentional photomodification of cells and tissues (Invited Paper)
Paper 11244-17
Author(s): Alfred Vogel, Xiao-Xuan Liang, Sebastian Freidank, Norbert Linz, Univ. zu Lübeck (Germany)
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Fluorescence lifetime trajectory of the mouse pre-implantation embryo using the phasor approach predicts its viability (Invited Paper)
Paper 11244-18
Author(s): Michelle Digman, Univ. of California, Irvine (United States)
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Eliminating drug-resistant bacteria and fungal infections via one-photon and multi-photon bleaching of intrinsic chromophores (Invited Paper)
Paper 11244-19
Author(s): Ji-Xin Cheng, Boston Univ. (United States)
Show Abstract
Session PSun:
Posters-Sunday
Sunday 2 February 2020
5:30 PM - 7:00 PM
Session Chairs:
Holly Aaron, Univ. of California, Berkeley (United States) ;
Lingyan Shi, Univ. of California, San Diego (United States) ;
Paolo Bianchini, Istituto Italiano di Tecnologia (Italy) ;
Alzbeta Marček Chorvátová, International Laser Ctr. (Slovakia)

Conference attendees are invited to attend the BiOS poster session on Sunday evening. Come view the posters, enjoy light refreshments, ask questions, and network with colleagues in your field.

Poster Setup: Sunday 10:00 AM – 4:30 PM
View poster presentation guidelines and set-up instructions at
http://spie.org/PWPosterGuidelines
A high-fat diet impacts collagen organization in breast tumor tissues but not in healthy ones
Paper 11244-67
Author(s): Yang Zhang, Zhiyi Liu, Tufts Univ. (United States); Lisa Arendt, Univ. of Wisconsin-Madison (United States); Irene Georgakoudi, Tufts Univ. (United States)
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Multicolor imaging of endogenous and exogenous fluorophores in living zebrafish larvae
Paper 11244-68
Author(s): Marco Andreana, Medizinische Univ. Wien (Austria); Caterina Sturtzel, St. Anna Kinderkrebsforschung e.V. (Austria); Wolfgang Drexler, Medizinische Univ. Wien (Austria); Martin Distel, St. Anna Kinderkrebsforschung e.V. (Austria); Angelika Unterhuber, Medizinische Univ. Wien (Austria)
Show Abstract
Automated spherical aberration correction applied to multiphoton microscopy
Paper 11244-69
Author(s): Carlo-Amadeo C. Alonzo, Olympus Corp. of the Americas (United States)
Show Abstract
Bleed-through elimination method in a dual-channel fluorescence microscopy system
Paper 11244-70
Author(s): Reddikumar Maddipatla, Patrice Tankam, Indiana Univ. (United States)
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Time-resolved anisotropy for cellular metabolic interpretations
Paper 11244-71
Author(s): Jenu V. Chacko, Kevin W. Eliceiri, Univ. of Wisconsin-Madison (United States)
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Ambient-light-on multiphoton microscopy by optical parametric amplification
Paper 11244-72
Author(s): Yi Sun, Haohua Tu, Sixian You, Chi Zhang, Yuan-Zhi Liu, Stephen A. Boppart, Univ. of Illinois (United States)
Show Abstract
Multiphoton microscopy in assessment of heterogeneous tissue engineered grafts
Paper 11244-73
Author(s): Daria Kuznetsova, Privolzhsky Research Medical Univ. (Russian Federation); Anastasia Koroleva, Laser Zentrum Hannover e.V. (Germany); Vadim V. Elagin, Privolzhsky Research Medical Univ. (Russian Federation); Boris Chichkov, Leibniz Univ. Hannover (Germany); Peter S. Timashev, Sechenov Univ. (Russian Federation); Elena V. Zagaynova, Privolzhsky Research Medical Univ. (Russian Federation)
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In vivo time-series quantitative evaluation of skin burn healing using second-harmonic-generation microscopy
Paper 11244-74
Author(s): Eiji Hase, Tokushima Univ. (Japan); Ryosuke Tanaka, Shuichiro Fukushima, Osaka Univ. (Japan); Takeshi Yasui, Tokushima Univ. (Japan), Osaka Univ. (Japan)
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Effect of wavelength on polarization-resolved second-harmonic-generation microscopy in thick tissues
Paper 11244-75
Author(s): Eiji Hase, Kosuke Maeda, Takahiko Mizuno, Takeo Minamikawa, Takeshi Yasui, Tokushima Univ. (Japan)
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Nonlinear imaging using a 35 fs 3.5 nJ all-PM fiber laser frequency doubled at 800 nm
Paper 11244-76
Author(s): Charles-Henri Hage, Simon Boivinet, Sébastien Vidal, Guillaume Machinet, Johan Boullet, ALPhANOV (France)
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Multiphoton imaging of precisely cut tumor slices for drug testing
Paper 11244-77
Author(s): Hsu-Cheng Huang, Shu-Han Wen, Shu-Jen Chiang, National Taiwan Univ. (Taiwan); Pei-Jung Lee, Huei-Wen Chen, National Taiwan Univ. College of Medicine (Taiwan); Shih-Chi Chen, The Chinese Univ. of Hong Kong (Hong Kong, China); Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
Show Abstract
Multiphoton imaging of dye penetration dynamics in tissue sections
Paper 11244-78
Author(s): Paul Tseng, Shu-Jen Chiang, National Taiwan Univ. (Taiwan); Shean-Jen Chen, National Chiao Tung Univ. (Taiwan); Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
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Two photon excitation in neurosciences using fiber lasers operating at 920nm and 1064nm
Paper 11244-79
Author(s): Pascal Dupriez, Spark Lasers (France)
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Automated Gleason grading of prostate cancers via deep learning in label-free multiphoton microscopic images
Paper 11244-80
Author(s): Xiaoqin Zhu, Jianyong Cai, Qinqin Yang, Zhexin Xu, Fujian Normal Univ. (China); Hong Chen, Fujian Medical Univ. (China)
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Multi-photon excited Fourier-transform fluorescence recovery after photobleaching (FT-FRAP) with patterned illumination
Paper 11244-81
Author(s): Andreas C. Geiger, Purdue Univ. (United States)
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Electric field imaging with vibrationally-resonant electric field-induced sum-frequency generation
Paper 11244-82
Author(s): Evan P. Perillo, NanoString Technologies, Inc. (United States); Mary E. Phipps, Los Alamos National Lab. (United States); Jennifer Martinez, Northern Arizona Univ. (United States); Anatoly Efimov, Los Alamos National Lab. (United States)
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Second-harmonic generation scattering from breast cancer core needle biopsies is associated with neoadjuvant chemotherapy response
Paper 11244-83
Author(s): Danielle Desa, Monisha Bhanote, Univ. of Rochester (United States); Robert L. Hill, Harmonigenic™ Corp. (United States); Joseph B. Majeski, Univ. of Rochester (United States); Brandon Buscaglia, Marcus D'Aguiar, Rochester Institute of Technology (United States); Robert L. Strawderman, David G. Hicks, Bradley M. Turner, Edward B. Brown, Univ. of Rochester (United States)
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Study of the sarcomeric addition process in a tissue-like cell construct under mechanical overload via TPEF-SHG imaging system
Paper 11244-85
Author(s): Ailin Wei, Zhonghai Wang, Zongming Yang, Shenghao Tan, Tong Ye, Clemson Univ. (United States); Yonghong Shao, Shenzhen Univ. (China); Thomas K. Borg, Medical Univ. of South Carolina (United States); Bruce Z. Gao, Clemson Univ. (United States)
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Autofluorescence imaging of metabolic changes in human plasma-like medium vs standard culture medium
Paper 11244-86
Author(s): Logan Florek, Morgridge Institute for Research (United States); Amani A. Gillette, Kimberly Huggler, Univ. of Wisconsin-Madison (United States); Jason Cantor, Melissa C. Skala, Univ. of Wisconsin-Madison (United States), Morgridge Institute for Research (United States)
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Confocal Mueller matrix imaging microscopy to study the corneal polarization anisotropies
Paper 11244-88
Author(s): Ilyas Saytashev, Sudipta Saha, Joseph Chue-Sang, Jessica C. Ramella-Roman, Florida International Univ. (United States)
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Monitoring drug induced changes in cardiomyocyte contractility with second harmonic generation (SHG) microscopy
Paper 11244-89
Author(s): Che-Wei Chang, Yao-Hui Sun, Hillary K. Kao, Dalyir I. Pretto, Deborah K. Lieu, James W. Chan, Univ. of California, Davis (United States)
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Inetic analysis of DNA and dox binding process using fluorescence lifetime imaging
Paper 11244-90
Author(s): Liwei Liu, Shenzhen Univ. (China)
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Multiphoton induced fluorescence and second harmonic imaging of drug-infused arterial walls for non-invasive photo-polymerizable stents
Paper 11244-91
Author(s): Tochukwu Emeakaroha, Divya Kota, South Dakota School of Mines and Technology (United States); Sam Young, Alumend, LLC (United States); Steve J. Smith, South Dakota School of Mines and Technology (United States); Ron Utecht, Alumend, LLC (United States)
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Label-free whole-colony imaging for metastatic and metabolic analysis by a flexible multimodal microscopy
Paper 11244-92
Author(s): Binglin Shen, Shenzhen Univ. (China)
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High-speed, large field-of-view and deep multiphoton imaging with an adaptive excitation source
Paper 11244-93
Author(s): Bo Li, Chunyan Wu, Mengran Wang, Kriti Charan, Chris Xu, Cornell Univ. (United States)
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Imaging plasma membrane microviscosity in cancer cells during chemotherapy
Paper 11244-94
Author(s): Liubov E. Shimolina, Privolzhsky Research Medical Univ. (Russian Federation), N.I. Lobachevsky State Univ. of Nizhni Novgorod (Russian Federation); Marina V. Shirmanova, Privolzhsky Research Medical Univ. (Russian Federation); Marina Kuimova, Imperial College London (United Kingdom); Maria M. Lukina, Nadezhda I. Ignatova, Elena V. Zagaynova, Privolzhsky Research Medical Univ. (Russian Federation)
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Monitoring the role of palmitic acid in glioma cells using stimulated Raman scattering microscopy
Paper 11244-84
Author(s): Yuhao Yuan, Niraj Shah, Fa-Ke Lu, Binghamton Univ. (United States)
Show Abstract
Session PLN:
BiOS Sunday Plenary
Sunday 2 February 2020
7:15 PM - 8:00 PM
Monday 3 February Show All Abstracts
Session 5:
Metabolism/NADH/FAD/Tryptophan
Monday 3 February 2020
8:00 AM - 10:15 AM
Session Chairs:
Elena V. Zagaynova, Privolzhsky Research Medical Univ. (Russian Federation) ;
Fu-Jen Kao, National Yang-Ming Univ. (Taiwan)
Metabolic imaging by simultaneous 2-photon FLIM of NAD(P)H and FAD (Invited Paper)
Paper 11244-20
Author(s): Wolfgang Becker, Becker & Hickl GmbH (Germany); Angelika C. Rueck, Univ. Ulm (Germany)
Show Abstract
Metabolic FLIM and oxygen PLIM: Multiphoton luminescence lifetime imaging on the way to clinical diagnosis (Invited Paper)
Paper 11244-21
Author(s): Angelika C. Rueck, Univ. Ulm (Germany); P. Schäfer, The Children's Hospital of Philadelphia (United States); Bjorn von Einem, Christine A. F. von Arnim, Sviatlana Kalinina, Univ. Ulm (Germany)
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Multiphoton imaging of liver pathology and regeneration (Invited Paper)
Paper 11244-22
Author(s): Elena V. Zagaynova, Privolzhsky Research Medical Univ. (Russian Federation); Svetlana A. Rodimova, Privolzhsky Research Medical Univ. (Russian Federation), N.I. Lobachevsky State Univ. of Nizhni Novgorod (Russian Federation); Daria Kuznetsova, Privolzhsky Research Medical Univ. (Russian Federation); Dmitry Reunov, Privolzhsky Research Medical Univ. (Russian Federation), N.I. Lobachevsky State Univ. of Nizhni Novgorod (Russian Federation); Alexander Gulin, N.N. Semenov Institute of Chemical Physics (Russian Federation); Varvara V. Dudenkova, Privolzhsky Research Medical Univ. (Russian Federation); Nikolay Bobrov, The Volga District Medical Ctr. of Federal Medical and Biological Agency (Russian Federation); Natalia Vdovina, Vladimir E. Zagainov, Privolzhsky Research Medical Univ. (Russian Federation)
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Probing tumor metabolism by FLIM of NAD(P)H (Invited Paper)
Paper 11244-23
Author(s): Marina V. Shirmanova, Maria M. Lukina, Liubov E. Shimolina, Irina N. Druzhkova, Nadezhda I. Ignatova, Privolzhsky Research Medical Univ. (Russian Federation); Vladimir E. Zagainov, The Volga District Medical Ctr. (Russian Federation); Varvara V. Dudenkova, Privolzhsky Research Medical Univ. (Russian Federation); Vladislav I. Shcheslavskiy, Becker & Hickl GmbH (Germany); Elena V. Zagaynova, Privolzhsky Research Medical Univ. (Russian Federation)
Show Abstract
Label free two photon microscopic imaging to assess tissue metabolic and biomechanical function (Invited Paper)
Paper 11244-24
Author(s): Irene Georgakoudi, Dimitra Pouli, Zhiyi Liu, Yang Zhang, Li Zeng, Lauren Black, Tufts Univ. (United States); Ana Soto, Carlos Sonnenschein, Tufts Univ. School of Medicine (United States); Hong-Thao Thieu, Elizabeth Genega, Tufts Medical Ctr. (United States)
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NADH and FAD excitation with one excitation wavelength (Invited Paper)
Paper 11244-25
Author(s): Ruofan Cao, Horst K. Wallrabe, Ammasi Periasamy, Univ. of Virginia (United States)
Show Abstract
The effects of T cell polarization and changes in microenvironment on cell autofluorescence
Paper 11244-26
Author(s): Kelsey Tweed, Morgridge Institute for Research (United States), Univ. of Wisconsin-Madison (United States); Isabel Jones, Morgridge Institute for Research (United States); Alex J. Walsh, Morgridge Institute for Research (United States), Texas A&M Univ. (United States); Steven M. Trier, Morgridge Institute for Research (United States); Melissa C. Skala, Morgridge Institute for Research (United States), Univ. of Wisconsin-Madison (United States)
Show Abstract
JenLab Young Investigator Award
Monday 3 February 2020
10:35 AM - 11:15 AM
Session Chair:
Ammasi Periasamy, Univ. of Virginia (United States)
Session 6:
Technology and In Vivo Imaging I
Monday 3 February 2020
11:15 AM - 12:15 PM
Session Chair:
Michelle Digman, Univ. of California, Irvine (United States)
Multiphoton and FLIM imaging in quantifying ex vivo and in vivo body organ kinetics of solutes (Invited Paper)
Paper 11244-27
Author(s): Michael S. Roberts, Amy Holmes, Univ. of South Australia (Australia); Haolu Wang, Xiaowen Liang, Xin Liu, The Univ. of Queensland (Australia); Hauke Studier, Becker & Hickl GmbH (Germany); Michael Pastore, Genoskin (France); Deborah Barkauskas, Jeff E. Grice, Yousuf S. Mohammed, The Univ. of Queensland (Australia)
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Quantification of blood-brain barrier permeability with multiphoton fluorescence imaging (Invited Paper)
Paper 11244-28
Author(s): Lingyan Shi, Univ. of California, San Diego (United States)
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Ultracompact fiber-optic scanning platform for high-speed two-photon neural imaging on freely-walking mice (Invited Paper)
Paper 11244-29
Author(s): Xingde Li, Johns Hopkins Univ. (United States)
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Lunch Break 12:15 PM - 1:15 PM
Session 7:
Technology and In Vivo Imaging II
Monday 3 February 2020
1:15 PM - 2:50 PM
Session Chairs:
Alberto Diaspro, Istituto Italiano di Tecnologia (Italy) ;
Paolo Bianchini, Istituto Italiano di Tecnologia (Italy)
Approaching two-photon excitation microscopy from PCM2000 confocal to PRISM image scanning system. (Invited Paper)
Paper 11244-30
Author(s): Alberto Diaspro, Istituto Italiano di Tecnologia (Italy), Univ. degli Studi di Genova (Italy)
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Large area functional and structural nonlinear brain imaging (Invited Paper)
Paper 11244-31
Author(s): Francesco Saverio Pavone, LENS - Lab. Europeo di Spettroscopie Non-Lineari (Italy)
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Easy two-photon image-scanning microscopy with SPAD array and blind image reconstruction (Invited Paper)
Paper 11244-32
Author(s): Sami V. Koho, Eli Slenders, Giorgio Tortarolo, Marco Castello, Istituto Italiano di Tecnologia (Italy); Mauro Buttafava, Federica A. Villa, Politecnico di Milano (Italy); Elena Tcarenkova, Univ. of Turku (Finland); Marcel Ameloot, Univ. Hasselt (Belgium); Paolo Bianchini, Colin J. R. Sheppard, Alberto Diaspro, Istituto Italiano di Tecnologia (Italy); Alberto Tosi, Politecnico di Milano (Italy); Giuseppe Vicidomini, Istituto Italiano di Tecnologia (Italy)
Show Abstract
Improving the resolution in multiphonton microscopy (Invited Paper)
Paper 11244-33
Author(s): Paolo Bianchini, Istituto Italiano di Tecnologia (Italy); Behjat Kariman, Istituto Italiano di Tecnologia (Italy), Univ. degli Studi di Genova (Italy); Francesco Garzella, Eleonora Uriati, Istituto Italiano di Tecnologia (Italy), Univ. degli Studi di Parma (Italy); Giulia Zanini, Takahiro Deguchi, Istituto Italiano di Tecnologia (Italy); Alberto Diaspro, Istituto Italiano di Tecnologia (Italy), Univ. degli Studi di Genova (Italy)
Show Abstract
Simultaneous dual-plane imaging with a multi-site mesoscope (Invited Paper)
Paper 11244-34
Author(s): Peter Saggau, Baylor College of Medicine (United States); Dmitri A. Tsyboulski, Janelia Farm Research Campus, Howard Hughes Medical Institute (United States); Natalia Orlova, Allen Institute for Brain Science (United States)
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Award Presentation
Monday 3 February 2020
2:50 PM - 3:10 PM
Session 8:
SHG/THG Microscopy
Monday 3 February 2020
3:30 PM - 5:15 PM
Session Chair:
Paul J. Campagnola, Univ. of Wisconsin-Madison (United States)
Evaluation of newly synthesized potential NLO-phores for 2-photon and SHG imaging. (Invited Paper)
Paper 11244-37
Author(s): Alzbeta Marček Chorvátová, International Laser Ctr. (Slovakia), Univ. of SS. Cyril and Methodius (Slovakia); Peter Magdolen, Andrea Martinicka, Comenius Univ. in Bratislava (Slovakia); Martin Uherek, Dusan Chorvat, International Laser Ctr. (Slovakia)
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Studying intrinsic skin aging by slide-free in vivo harmonic generation microscopy (Invited Paper)
Paper 11244-36
Author(s): Chi-Kuang Sun, Kuan-Hung Lin, Ming-Liang Wei, National Taiwan Univ. (Taiwan); Yi-Hua Liao, National Taiwan Univ. Hospital (Taiwan)
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Multiscale SHG analysis of collagen architecture alterations in human idiopathic pulmonary fibrosis (Invited Paper)
Paper 11244-35
Author(s): Paul J. Campagnola, Darian S. James, Zachary Alden, Hsin-Yu Chang, Univ. of Wisconsin-Madison (United States); Mark Jones, Univ. of Southampton (United Kingdom); Nathan Sandbo, Univ. of Wisconsin-Madison (United States)
Show Abstract
Label-free characterization of attenuation lengths of cortical regions via three-photon microscopy in awake mice
Paper 11244-38
Author(s): Murat Yildirim, Ming Hu, Peter T. C. So, Mriganka Sur, Massachusetts Institute of Technology (United States)
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Second harmonic generation imaging collagen structure modulation in embryonic chicken cornea
Paper 11244-39
Author(s): Sheng-Lin Lee, Ming-Ye He, Yang-Fang Chen, Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
Show Abstract
Bringing third and second harmonic generation microscopy into the clinic for the assessment of fresh lung (tumor) tissue
Paper 11244-40
Author(s): Laura M. G. Van Huizen, Vrije Univ. Amsterdam (Netherlands); Daniëlle Seinstra, Chris Dickhoff, Teodora Radonic, Idris Bahce, Amsterdam UMC (Netherlands); Frank van Mourik, Tritos Diagnostics B.V. (Netherlands); Jouke T. Annema, Johannes M. A. Daniels, Wim-Jan P. van Boven, Amsterdam UMC (Netherlands); Marie Louise Groot, Vrije Univ. Amsterdam (Netherlands)
Show Abstract
Tuesday 4 February Show All Abstracts
Session 9:
FLIM/FRET/FCS
Tuesday 4 February 2020
8:00 AM - 10:00 AM
Session Chair:
Kevin W. Eliceiri, Univ. of Wisconsin-Madison (United States)
Two-photon phosphorescence lifetime microscopy (2PLM) of xxygen (Invited Paper)
Paper 11244-41
Author(s): Sergei A. Vinogradov, Univ. of Pennsylvania (United States)
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FLIM, FRET and high content analysis (HCA) (Invited Paper)
Paper 11244-42
Author(s): Paul M. W. French, Imperial College London (United Kingdom)
Show Abstract
Multi-target immunofluorescence using spectral FLIM-FRET for separation of undesirable antibody cross-labeling and autofluorescence (Invited Paper)
Paper 11244-43
Author(s): Sumeet Rohilla, PicoQuant GmbH (Germany), Charité Universitätsmedizin Berlin (Germany); Benedikt Krämer, Felix Koberling, Uwe Ortmann, PicoQuant GmbH (Germany); Ingo Gregor, Georg-August-Univ. Göttingen (Germany); Andreas C. Hocke, Charité Universitätsmedizin Berlin (Germany); Rainer Erdmann, PicoQuant GmbH (Germany)
Show Abstract
Monitoring receptor-ligand interactions using fluorescence lifetime FRET imaging via deep learning (Invited Paper)
Paper 11244-44
Author(s): Margarida Barroso, Alena Rudkouskaya, Albany Medical College (United States); Jason T. Smith, Xavier Intes, Rensselaer Polytechnic Institute (United States)
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High speed 2-photon fluorescence lifetime imaging of protein-protein interactions with a swept array microscope (Invited Paper)
Paper 11244-45
Author(s): Simon M. Ameer-Beg, Simon P. Poland, Thomas Kavanagh, James A. Levitt, King's College London (United Kingdom); Robert K. Henderson, Hanning Mai, The Univ. of Edinburgh (United Kingdom); Andrea Serio, Conor Treacy, Kelly O'Toole, King's College London (United Kingdom); Richard Walker, Photon Force Ltd. (United Kingdom)
Show Abstract
Live cell metabolic imaging of cancer cell lines using multiphoton fluorescence polarization (Invited Paper)
Paper 11244-46
Author(s): Einstein Gnanatheepam, Anandh Sundaramoorthy, Durgalakshmi Dhinasekaran, Aruna Prakasa Rao, Ganesan Singaravelu, Anna Univ., Chennai (India)
Show Abstract
Session 10:
Technology and In Vivo Imaging III
Tuesday 4 February 2020
10:30 AM - 12:15 PM
Session Chair:
Margarida Barroso, Albany Medical College (United States)
Time-resolved mesoscopic imaging of a whole animal by FastFLIM (Invited Paper)
Paper 11244-47
Author(s): Yuansheng Sun, ISS, Inc. (United States); Chien Sing Poon, Wright State Univ. (United States); Hailin Qiu, Orient KOJI Scientific (China); Ulas C. Coskun, Shih-Chu J. Liao, Sunil Shah, Beniamino Barbieri, ISS, Inc. (United States); Ulas Sunar, Wright State Univ. (United States)
Show Abstract
Real time imaging of the multiphoton excitation volume using a point detector (Invited Paper)
Paper 11244-48
Author(s): Sudipta Maiti, Tata Institute of Fundamental Research (India)
Show Abstract
Synchronized subharmonic modulation in stimulated emission microscopy (Invited Paper)
Paper 11244-49
Author(s): Fu-Jen Kao, National Yang-Ming Univ. (Taiwan)
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Multiplexed 3-photon microscopy for functional connectomics of mammalian cortex
Paper 11244-50
Author(s): Kevin Takasaki, Allen Institute for Brain Science (United States); Dmitri A. Tsyboulski, Howard Hughes Medical Institute (United States); Jack Waters, Allen Institute for Brain Science (United States)
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High speed resonant fiber-optic scanning nonlinear endomicroscopy for monitoring brain functional dynamics
Paper 11244-51
Author(s): Hyeon-Cheol Park, Honghua Guan, Ang Li, Dawei Li, Johns Hopkins Univ. (United States); Yuanlei Yue, The George Washington Univ. (United States); Ming-Jun Li, Corning Incorporated (United States); Hui Lu, The George Washington Univ. (United States); Xingde Li, Johns Hopkins Univ. (United States)
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NAD(P)H FLIM for metabolic imaging: Fast acquisition, faster analysis, fastest decay detection
Paper 11244-52
Author(s): Lukas Z. Braun, Axel Bergmann, Becker & Hickl GmbH (Germany); Rodrigo Suarez Ibarrola, Universitätsklinikum Freiburg (Germany); Hauke Studier, Becker & Hickl GmbH (Germany); Philippe F. Pohlmann, Universitätsklinikum Freiburg (Germany); Wolfgang Becker, Becker & Hickl GmbH (Germany)
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Lunch Break 12:15 PM - 1:30 PM
Session 11:
Technology and Raman Microscopy
Tuesday 4 February 2020
1:30 PM - 3:10 PM
Session Chair:
Lingyan Shi, Univ. of California, San Diego (United States)
From multi-photon absorption and stimulated emission to forbidden S0→T1 transition (Invited Paper)
Paper 11244-53
Author(s): Zygmunt K. Gryczynski, Texas Christian Univ. (United States); Ignacy Gryczynski, Univ. of North Texas Health Science Ctr. at Fort Worth (United States)
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Coherent Raman scattering imaging for pharmacokinetics and pharmacodynamics (Invited Paper)
Paper 11244-54
Author(s): Conor L. Evans, Wellman Ctr. for Photomedicine (United States)
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High-speed imaging during femtosecond-laser cell optoporation
Paper 11244-55
Author(s): Ana Maria Gonçalves Batista, Hans Georg Breunig, Karsten König, Univ. des Saarlandes (Germany)
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Improving the sensitivity of coherent Raman scattering microscopy with 10 MHz solid-state light source
Paper 11244-56
Author(s): Wenlong Yang, Gary R. Holtom, Harvard Univ. (United States); Yuanzhen Suo, Xiaoliang S. Xie, Peking Univ. (China)
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Frequency-tunable two-color ultrafast fiber laser for nonlinear excitation of NADH and FAD
Paper 11244-57
Author(s): Axel Friedenauer, Konrad Birkmeier, Andreas Brodschelm, Christoph Skrobol, Patrick Leisching, TOPTICA Photonics AG (Germany)
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Time-lapse stimulated Raman scattering (SRS) microscopy
Paper 11244-58
Author(s): Yuhao Yuan, Soumit Saha, Mohammad I. Almohaisin, Steven T. Blum, Fa-Ke Lu, Binghamton Univ. (United States)
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Session 12:
Technology and In Vivo Imaging IV
Tuesday 4 February 2020
3:30 PM - 5:35 PM
Session Chairs:
Yuansheng Sun, ISS, Inc. (United States) ;
Hauke Studier, Becker & Hickl GmbH (Germany)
High-resolution tissue imaging using moxifloxacin as a clinically compatible cell labeling agent (Invited Paper)
Paper 11244-59
Author(s): Ki Hean Kim, Seunghun Lee, Bumju Kim, Pohang Univ. of Science and Technology (Korea, Republic of)
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Multiphoton FLIM is gaining ground as a clinical tool
Paper 11244-60
Author(s): Hauke Studier, Becker & Hickl GmbH (Germany); Yousuf S. Mohammed, Michael S. Roberts, The Univ. of Queensland (Australia); Amy Holmes, Michael Pastore, Univ. of South Australia (Australia); Wolfgang Becker, Becker & Hickl GmbH (Germany)
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Assessing functional and morphological properties of different adipose tissues in vivo by high-resolution, quantitative multi-photon imaging using endogenous contrast
Paper 11244-61
Author(s): Zhiyi Liu, Dimitra Pouli, Yang Zhang, Irene Georgakoudi, Tufts Univ. (United States)
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Flexible multiphoton microscopy with femtosecond-pulse fiber delivery
Paper 11244-62
Author(s): Hans Georg Breunig, Univ. des Saarlandes (Germany), JenLab GmbH (Germany); Karsten König, Univ. des Saarlandes (Germany)
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Optimization of analysis protocol and probe expression for monitoring intracellular oxygenation using fluorescence lifetime imaging of myoglobin-mCherry
Paper 11244-63
Author(s): Rozhin Penjweini, Greg Alspaugh, Branden Roarke, Alexandra Chand, Jay R. Knutson, National Institutes of Health Clinical Ctr. (United States)
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Multimodal method for the identification of multiphoton cancer biomarkers using 3D tumor spheroids
Paper 11244-64
Author(s): Björn-Ole Meyer, M. Pilar J. Stella, Dominik Marti, DTU Health Tech (Denmark); Bjørn Holst, Boye S. Nielsen, Kim Holmstrøm, Bioneer A/S (Denmark); Peter E. Andersen, DTU Health Tech (Denmark)
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Multiphoton autofluorescence imaging of advanced glycation end products glycated tissues
Paper 11244-65
Author(s): Chih-Ju Lin, National Taiwan Univ. (Taiwan); Jeon Woong Kang, Peter T. C. So, Massachusetts Institute of Technology (United States); Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
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Rapid volumetric multiphoton microscopy via dual resonant scanning with deep learning
Paper 11244-66
Author(s): Chia Wei Hsu, Yu-Shin Hsu, Chun-Yu Lin, Shean-Jen Chen, National Chiao Tung Univ. (Taiwan)
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