Digital Forum
Online Only
6 - 11 March 2021
Conference 11648
Multiphoton Microscopy in the Biomedical Sciences XXI
Conference Committee
Important Dates
Abstract Due:
26 August 2020

Author Notification:
9 November 2020

Manuscript Due Date:
12 February 2021

Conference Cosponsors












Additional Conference Information
This conference is accepting post-deadline submissions:
Post-Deadline Submission Portal
Monday 18 January Show All Abstracts
Session R: Welcome and Introduction
Welcome and Introduction to Conference 11648
Paper 11648-800
Author(s): Ammasi Periasamy, Univ. of Virginia (United States); Peter T. C. So, Massachusetts Institute of Technology (United States); Karsten König, JenLab GmbH (Germany)
Show Abstract
Session 1: Keynote Session
Free-electron and thermally mediated pathways of photodamage in nonlinear microscopy (Keynote Presentation)
Paper 11648-1
Author(s): Alfred Vogel, Xiao-Xuan Liang, Sebastian Freidank, Norbert Linz, Univ. zu Lübeck (Germany)
Show Abstract
Multiphoton autofluorescence imaging of immune cells in cancer (Keynote Presentation)
Paper 11648-2
Author(s): Melissa C. Skala, Tiffany M. Heaster, Kelsey Tweed, Morgridge Institute for Research (United States), Univ. of Wisconsin (United States); Alexa Heaton, Morgridge Institute for Research (United States); Dan Pham, Morgridge Institute for Research (United States), Univ. of Wisconsin (United States); Katherine Mueller, Dave Beebe, Paul Sondel, Krishanu Saha, Univ. of Wisconsin-Madison (United States)
Show Abstract
Adaptive optics for high-resolution multiphoton microscopy (Keynote Presentation)
Paper 11648-3
Author(s): Na Ji, Univ. of California, Berkeley (United States)
Show Abstract
Session 2: FLIM/FRET/FCS I
Getting more from TCSPC FLIM data: SPCImage NG next generation data analysis software (Invited Paper)
Paper 11648-4
Author(s): Wolfgang H. Becker, Vladislav Shcheslavskiy, Axel Bergmann, Becker & Hickl GmbH (Germany)
Show Abstract
Skin cancer detection with a compact multimodal fiber laser multiphoton FLIM tomograph (Invited Paper)
Paper 11648-5
Author(s): Karsten König, JenLab GmbH (Germany), Univ. des Saarlandes (Germany); Dmitry Pankin, JenLab GmbH (Germany); Holger Hänssle, Julia Winkler, UniversitätsKlinikum Heidelberg (Germany); Michael Zieger, Martin Kaatz, SRH Wald-Klinikum Gera GmbH (Germany)
Show Abstract
Multiplexing FRET by PIE-FastFLIM and the phasor plots (Invited Paper)
Paper 11648-6
Author(s): Yuansheng Sun, ISS, Inc. (United States); Richard N. Day, Indiana Univ. School of Medicine (United States); Shih-Chu Liao, Beniamino Barbieri, ISS, Inc. (United States)
Show Abstract
Quantitation of cerebral oxygen tension using phasor analysis and phosphorescent lifetime imaging microscopy (Invited Paper)
Paper 11648-7
Author(s): Amanda Chisholm, Chang Liu, Northeastern Univ. (United States); Buyin Fu, Clover Su, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States); Ikbal Sencan, Athinoula A. Martinos Ctr. for Biomedical Imaging, Massachusetts General Hospital (United States), Harvard Medical School (United States); Sava Sakadžic, Athinoula A. Martinos Ctr. for Biomedical Imaging (United States); Mohammad A. Yaseen, Northeastern Univ. (United States)
Show Abstract
Visualizing dynamic processes with rapidFLIMHiRes, the ultra fast FLIM imaging method with outstanding 10ps time resolution (Invited Paper)
Paper 11648-8
Author(s): Maria Loidolt-Krüger, Fabian Jolmes, Matthias Patting, Michael Wahl, Evangelos Sismakis, André Devaux, Uwe Ortmann, Felix Koberling, Rainer Erdmann, PicoQuant GmbH (Germany)
Show Abstract
Session 3: FLIM/FRET/FCS II
Continuous and discrete phasor analysis of binned or time-gated periodic decays
Paper 11648-9
Author(s): Xavier Michalet, Univ. of California, Los Angeles (United States)
Show Abstract
Functional imaging progress for clinical use
Paper 11648-10
Author(s): Hauke Studier, Becker & Hickl GmbH (Germany); Amy Holmes, Michael Pastore, Univ. of South Australia (Australia); Wolfgang H. Becker, Becker & Hickl GmbH (Germany); Michael S. Roberts, Univ. of South Australia (Australia)
Show Abstract
Line scanning mechanical streak camera for high-throughput parallel phosphorescence lifetime imaging
Paper 11648-11
Author(s): Meng Cui, Purdue Univ. (United States)
Show Abstract
Two-photon phosphorescence lifetime microscopy of intestinal capillary pO2 and RBC flux
Paper 11648-12
Author(s): Ikbal Sencan, Athinoula A. Martinos Ctr. for Biomedical Imaging, Massachusetts General Hospital (United States), Harvard Medical School (United States); Wenjing Zong, Perelman School of Medicine, Univ. of Pennsylvania (United States); Mohammed A. Alfadhel, Buyin Fu, Athinoula A. Martinos Ctr. for Biomedical Imaging, Massachusetts General Hospital (United States); Mohammad A. Yaseen, Northeastern Univ. (United States); Gary D. Wu, Perelman School of Medicine, Univ. of Pennsylvania (United States); Sava Sakadžic, Athinoula A. Martinos Ctr. for Biomedical Imaging, Massachusetts General Hospital (United States), Harvard Medical School (United States); Sergei A. Vinogradov, Perelman School of Medicine, Univ. of Pennsylvania (United States)
Show Abstract
Session 4: Metabolism-NADH/FAD/Tryptophan
FLIM based optical redox ratio of NADH, FAD and FMN versus metabolic index of NADH. Improved algorithms for metabolic imaging (Invited Paper)
Paper 11648-17
Author(s): Angelika C. Rueck, Univ. Ulm (Germany)
Show Abstract
Metabolic FLIM at the dawn of clinical application (Invited Paper)
Paper 11648-18
Author(s): Wolfgang H. Becker, Lukas Braun, Becker & Hickl GmbH (Germany)
Show Abstract
Fluorescence lifetime imaging of metabolic co-enzymes to determine cell type and function (Invited Paper)
Paper 11648-19
Author(s): Alex J. Walsh, Linghao Hu, Elizabeth Cardona, Nianchao Wang, Jocelyn Martinez, Uyen Nguyen, Chris Weber, Naman Bala, Texas A&M Univ. (United States)
Show Abstract
Discrimination of urinary tract infection bacteria using multiphoton redox imaging (Invited Paper)
Paper 11648-20
Author(s): Anandh Sundaramoorthy, Einstein Gnanatheepam, Bharanidaran Ganesan, Durgalakshmi Dhinasekaran, Aruna Prakasa Rao, Ganesan Singaravelu, Anna Univ. Chennai (India)
Show Abstract
Autofluorescence Anisotropy based investigation of cellular heterogeneity and metabolism (Invited Paper)
Paper 11648-21
Author(s): Jenu V. Chacko, Mark A. Tsuchida, Han-Nim Lee, Univ. of Wisconsin-Madison (United States); Erich Grotewold, Michigan State Univ. (United States); Marisa S. Otegui, Univ. of Wisconsin-Madison (United States); Kevin Eliceiri, Univ. of Wisconsin-Madison (United States), Univ. of Wisconsin-Madison (United States)
Show Abstract
Ex vivo metabolic imaging of tumor samples from patients by FLIM
Paper 11648-22
Author(s): Maria M. Lukina, Lubov E. Shimolina, Varvara V. Dudenkova, Alena I. Gavrina, Vladimir E. Zagainov, Dmitriy V. Komarov, Konstantin S. Yashin, Igor A. Medyanik, Elena V. Zagaynova, Marina V. Shirmanova, Privolzhsky Research Medical Univ. (Russian Federation)
Show Abstract
Multiphoton fluorescence bioimaging of mammalian cells in presence of Zinc Nitride nanoparticle
Paper 11648-23
Author(s): Prabha Soundharraj, Durgalakshmi Dhinasekaran, Aruna Prakasa Rao, Ganesan Singaravelu, Anna Univ. Chennai (India)
Show Abstract
Session 5: Technology and Applications I
Microfluid setup for high throughput laser-assisted cell transfection (Invited Paper)
Paper 11648-29
Author(s): Karsten König, JenLab GmbH (Germany), Univ. des Saarlandes (Germany)
Show Abstract
Innovative light sources for biological imaging
Paper 11648-30
Author(s): Ian Read, MKS Instruments, Inc. (United States)
Show Abstract
Trends and developments in ultrafast lasers for non-linear microscopy
Paper 11648-31
Author(s): Darryl McCoy, Coherent Scotland Ltd. (United Kingdom); Marco Arrigoni, Coherent, Inc. (United States); Mantas Butkus, Coherent Scotland Ltd. (United Kingdom)
Show Abstract
Development and Characterization of Isocyanide Tags for IR and Raman based imaging (Invited Paper)
Paper 11648-32
Author(s): Richard C. Prince, Eric Potma, Univ. of California, Irvine (United States)
Show Abstract
Robust single-cell classification in hyperspectral stimulated raman scattering imaging by machine learning (Invited Paper)
Paper 11648-33
Author(s): Hyeon Jeong Lee, Zhejiang Univ. (China)
Show Abstract
Photon counting based pump-probe microscopy
Paper 11648-34
Author(s): Khalil Ur Rehman, Subir Das, Fu-Jen Kao, National Yang-Ming Univ. (Taiwan)
Show Abstract
Sectioning-free virtual H&E histology with fiber-based two-photon microscopy
Paper 11648-35
Author(s): Matthias Strauch, Jan Philip Kolb, Wolfgang Draxinger, Ann-Kathrin Popp, Melanie Wacker, Institut für Biomedizinische Optik, Univ. zu Lübeck (Germany); Nadine Merg, Jennifer Hundt, Lübecker Institut für Experimentelle Dermatologie, Univ. zu Lübeck (Germany); Sebastian Karpf, Robert Huber, Institut für Biomedizinische Optik, Univ. zu Lübeck (Germany)
Show Abstract
Session 6: SHG/THG Microscopy I
SHG and optical scattering analysis of collagen architecture alterations in human idiopathic pulmonary fibrosis (Invited Paper)
Paper 11648-24
Author(s): Paul J. Campagnola, Darian James, Alex Jambor, Emily Shelton, Nathan Sandbo, Univ. of Wisconsin-Madison (United States)
Show Abstract
Intratumoral heterogeneity of second-harmonic generation scattering from tumor collagen and its effects on metastatic risk prediction
Paper 11648-25
Author(s): Danielle E. Desa, Wencheng Wu, Univ. of Rochester (United States); Robert L. Hill, Harmonigenic™ Corp. (United States); J.W.M. Martens, Erasmus MC (Netherlands); Robert L. Strawderman, Bradley M. Turner, Edward B. Brown, Univ. of Rochester (United States)
Show Abstract
Translation of third and second harmonic generation microscopy into the clinic for the assessment of fresh lung tumor tissue
Paper 11648-26
Author(s): Laura M. Van Huizen, Vrije Univ. Amsterdam (Netherlands); Teodora Radonic, Amsterdam UMC (Netherlands); Frank van Mourik, Femto Diagnostics B.V. (Netherlands); Daniëlle Seinstra, Chris Dickhoff, Johannes M. A. Daniels, Idris Bahce, Jouke T. Annema, Amsterdam UMC (Netherlands); Marie Louise Groot, Vrije Univ. Amsterdam (Netherlands)
Show Abstract
Label-free imaging of biological tissue using widefield second-harmonic generation microscopy
Paper 11648-27
Author(s): Danielius Samsonas, Vilnius Univ. (Lithuania), Light Conversion Ltd. (Lithuania); Lukas Kontenis, Light Conversion Ltd. (Lithuania); Mikas Vengris, Vilnius Univ. (Lithuania)
Show Abstract
Role of collagen fiber morphology on ovarian cancer cell migration using multiphoton excited fabricated image-based models of the extracellular matrix (Invited Paper)
Paper 11648-28
Author(s): Paul J. Campagnola, Samuel Alkmin, Manish Patankar, Univ. of Wisconsin-Madison (United States)
Show Abstract
Session 7: Technology and Applications II
Flexible and compact multiphoton microscope with fiber delivered femtosecond-NIR laser pulses
Paper 11648-36
Author(s): Marek Warzecha, Arun Paudel, Sebastian Adam, JenLab GmbH (Germany); Hans Georg Breunig, Karsten König, Univ. des Saarlandes (Germany), JenLab GmbH (Germany)
Show Abstract
Portable nonlinear endoscope driven by an optimized compact femtosecond fiber-based laser source at 930nm
Paper 11648-37
Author(s): Sven Dobner, Carsten Cleff, Menlo Systems GmbH (Germany); Victor Tsvirkun, Aix Marseille Univ. (France), CNRS (France), Ecole Centrale de Marseille (France); Vasyl Mytskaniuk, Herve Rigneault, Aix Marseille Univ. (France), CNRS (France), Ecole Centrale de Marseille (France); Michael Mei, Ronald Holzwarth, Menlo Systems GmbH (Germany)
Show Abstract
Chemical complexity of the retina addressed by super-phasor analysis
Paper 11648-38
Author(s): Jessica Kline, Marcos Dantus, Michigan State Univ. (United States)
Show Abstract
Accurate measurement of red blood cell velocity with laser scanning microscopy
Paper 11648-39
Author(s): Emmanuelle M. Chaigneau, Serge Charpak, INSERM (France)
Show Abstract
Multiphoton imaging of induced autofluorescent advanced glycation end products in porcine aorta
Paper 11648-40
Author(s): Chih-Ju Lin, National Taiwan Univ. (Taiwan); Jeon Woong Kang, Peter T. C. So, Laser Biomedical Research Ctr., Massachusetts Institute of Technology (United States); Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
Show Abstract
Multiphoton imaging of the formation of fluorescent advanced glycation end products in glycated tissues
Paper 11648-41
Author(s): Chih-Ju Lin, National Taiwan Univ. (Taiwan); Jeon Woong Kang, Peter T. C. So, Laser Biomedical Research Ctr., Massachusetts Institute of Technology (United States); Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
Show Abstract
Session 8: In Vivo Imaging
Multimodal microscopy of the mouse brain in the 1700 nm optical window in vivo (Invited Paper)
Paper 11648-13
Author(s): Xusan Yang, Siyang Liu, Fei Xia, Meiqi Wu, Steven Adie, Chris Xu, Cornell Univ. (United States)
Show Abstract
Fast in vivo multiphoton microscopy using optimized light-sheet illumination (Invited Paper)
Paper 11648-14
Author(s): Vincent Maioli, Antoine Boniface, Pierre Mahou, Júlia Ferrer Ortas, Lamiae Abdeladim, Emmanuel Beaurepaire, Willy Supatto, Ecole Polytechnique (France)
Show Abstract
High power OPCPA system for in-vivo 2- and 3-photon brain imaging
Paper 11648-15
Author(s): Michael Schulz, Gregor Indorf, Torsten Golz, Ivanka Grguras, Thomas Braatz, Ekaterina Zapolnova, Jan Heye Buss, Robert Riedel, Class 5 Photonics GmbH (Germany)
Show Abstract
Experimental investigation of the depth limit in three-photon nicroscopy
Paper 11648-16
Author(s): Najva Akbari, Chris Xu, Cornell Univ. (United States)
Show Abstract
Session P: Poster Session
Intravital multiphoton imaging of the bacterium Group A Streptococcus in the skin
Paper 11648-42
Author(s): Angela Kurz, Centenary Institute (Australia)
Show Abstract
Convolutional neural network denoising in fluorescence lifetime imaging microscopy (FLIM)
Paper 11648-43
Author(s): Varun Mannam, Univ. of Notre Dame (United States); Yide Zhang, Univ. of Notre Dame (United States), Caltech (United States); Xiaotong Yuan, Univ. of Notre Dame (United States); Takashi Hato, Pierre C. Dagher, Indiana Univ. (United States); Evan L. Nichols, Cody J. Smith, Univ. of Notre Dame (United States); Kenneth W. Dunn, Indiana Univ. (United States); Scott Howard, Univ. of Notre Dame (United States)
Show Abstract
ABT-263 inhibition of Bcl-2 alters optical redox ratio measurements independent of oxygen consumption rates and viability
Paper 11648-44
Author(s): Amani A. Gillette, Rebecca DeStefanis, Dustin Deming, Univ. of Wisconsin-Madison (United States); Melissa C. Skala, Univ. of Wisconsin-Madison (United States), Morgridge Institute for Research (United States)
Show Abstract
Improvement in labeling efficiency of hydrophilic and hydrophobic fluorescent molecules in thick tissue sections
Paper 11648-45
Author(s): Po-Hang Tseng, Shu-Jen Chiang, National Taiwan Univ. (Taiwan); Shean-Jen Chen, National Chiao Tung Univ. (Taiwan); Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
Show Abstract
Multiphoton imaging of the effects of formaldehyde fixation on tissue autofluorescence
Paper 11648-46
Author(s): Shu-Jen Chiang, Po-Hang Tseng, National Taiwan Univ. (Taiwan); Shean-Jen Chen, National Chiao Tung Univ. (Taiwan); Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
Show Abstract
Machine learning architecture to predict drug response in cancer cells
Paper 11648-47
Author(s): Jiaxin Zhang, Ruofan Cao, Horst Wallrabe, Daniel Weller, Univ. of Virginia (United States); Ammasi Periasamy, Univ. of Virgina (United States)
Show Abstract
Novel pH-responsive highly fluorescent lipophilic coumarins as efficient two-photon sensors of acidic and basic environments
Paper 11648-48
Author(s): Matt Rammo, Aleksander Trummal, Charlie Stark, Meelis-Mait Sildoja, Merle Uudsemaa, Katrin Petritsenko, National Institute of Chemical Physics and Biophysics (Estonia); Aleksander Rebane, Montana State Univ. (United States); Juri Pahapill, National Institute of Chemical Physics and Biophysics (Estonia); Ivo Leito, Sigrid Selberg, Institute of Chemistry, Univ. of Tartu (Estonia)
Show Abstract
Mapping of metabolic functions in cancer and healthy cells: Study of the redox ratio, fluorescence lifetime, and cellular metabolism.
Paper 11648-49
Author(s): Giancarlo de Souza, Matheus L. O. Pena, Renan A. Romano, Cristina Kurachi, Univ. de São Paulo (Brazil)
Show Abstract
Miniature two-photon microscopy for enlarged field-of-view, multi-plane, and long-term brain imaging
Paper 11648-50
Author(s): Runlong Wu, Peking Univ. (China); Weijian Zong, Norwegian Univ. of Science and Technology (Norway); Shiyuan Chen, Junjie Wu, Peking Univ. (China); Zhe Zhao, Beijing Institute of Microbiology and Epidemiology (China); Guoqing Chen, Peking Univ. (China); Haitao Wu, Beijing Institute of Microbiology and Epidemiology (China); Yunfeng Zhang, Aimin Wang, Liangyi Chen, Heping Cheng, Peking Univ. (China)
Show Abstract
Metabolic imaging and secondary ion mass spectrometry analysis to investigate liver structure and function during regeneration
Paper 11648-51
Author(s): Daria Kuznetsova, Privolzhsky Research Medical Univ. (Russian Federation); Svetlana Rodimova, Privolzhsky Research Medical Univ. (Russian Federation), Lobachevsky State Univ. of Nizhny Novgorod (Russian Federation); Nikolai Bobrov, Privolzhsky Research Medical Univ. (Russian Federation), The Volga District Medical Ctr. (Russian Federation); Alexander Gulin, Semenov Institute of Chemical Physics (Russian Federation); Natalia Vdovina, Privolzhsky Research Medical Univ. (Russian Federation); Vladimir E. Zagainov, Privolzhsky Research Medical Univ. (Russian Federation), The Volga District Medical Ctr. (Russian Federation); Elena V. Zagaynova, Lobachevsky State Univ. of Nizhny Novgorod (Russian Federation), Privolzhsky Research Medical Univ. (Russian Federation)
Show Abstract
Conference Committee
Conference Chairs
Program Committee
  • Holly Aaron, Univ. of California, Berkeley (United States)
  • Margarida Barroso, Albany Medical College (United States)
  • Wolfgang Becker, Becker & Hickl GmbH (Germany)
  • Paul J. Campagnola, Univ. of Wisconsin-Madison (United States)
  • Ji-Xin Cheng, Purdue Univ. (United States)
  • Alberto Diaspro, Istituto Italiano di Tecnologia (Italy)
  • Michelle Digman, Univ. of California, Irvine (United States)
  • Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
  • Kevin W. Eliceiri, Univ. of Wisconsin-Madison (United States)
  • Scott Fraser, The Univ. of Southern California (United States)
  • Katsumasa Fujita, Osaka Univ. (Japan)
  • Enrico Gratton, Univ. of California, Irvine (United States)
  • Min Gu, RMIT Univ. (Australia)
  • Stefan W. Hell, Max-Planck-Institut für Biophysikalische Chemie (Germany)

Program Committee continued...
Back to Top
PREMIUM CONTENT
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?
close_icon_gray