Conference 11627B

Lasers in Dentistry XXVII

Digital Forum: On-demand starting 6 March
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  • Welcome and Introduction
  • BiOS Hot Topics
  • 1: Lasers in Dentistry
  • Poster Session
2021-03-02T19:16:42-08:00
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UPCOMING LIVE EVENTS:
Welcome and Introduction
11627-801
Author(s): Peter Rechmann, Daniel Fried, Univ. of California, San Francisco (United States)
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Welcome and Introduction to SPIE Photonics West BiOS conference 11627B: Lasers in Dentistry XXVII.
BiOS Hot Topics
11618-700
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Every year, attendees look forward to Saturday Night Hot Topics, an evening spent hearing highly engaged, world renowned speakers reveal the latest innovations in their areas of expertise. Don't miss this year's outstanding list of speakers.
11648-601
Author(s): Enrico Gratton, Univ. of California, Irvine (United States)
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11648-600
Author(s): Kevin K. Tsia, The Univ. of Hong Kong (Hong Kong, China)
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Session 1: Lasers in Dentistry
11627-11
CANCELED: 3D stereolithography print (SLA): surface and analysis in cross section in clinical orthodontic and dental applications
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The aim of our contribution was to evaluate some of 3D print indications from clinical practice with checking of the surface and cross section of model. Patients during clinical treatment were scanned by the optical scanner system 3Shape TRIOS 3R. The data from scanner were stored to computer, and transferred to the STL files and then stereolithographic models (Formlabs 3D PrinterForm 2) were prepared following manufacturer directions. The method of optical triangulation, photogrammetric methods and Fringe Projection was prepared. Surface and structure analysis was evaluated also with Alpha Step IQ profilometr and scanning electron microscope JSM 5510 LV. The deviation was approximately by about 0.1 mm. Surface was smooth and homogeneous and its quality was directly connected with type and structure of filaments. Clinical applications demonstrated unusual treatment options for patients with nausea, patients with rare diseases or with small space between upper and lower jaw.
11627-12
Author(s): Peter Rechmann, Maxwell Kubitz, Benjamin W. Chaffee, Beate M. T. Rechmann, Univ. of California, San Francisco (United States)
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The objective of this randomized, split-mouth controlled, clinical trial was to evaluate whether the use of a short-pulsed 9.3µm CO2-laser increases the caries resistance of occlusal pit and fissures in addition to fluoride therapy over 12-months. 60 participants were enrolled. Second molars were randomized into test and control. Test molars were irradiated with a 9.3μm CO2-laser. Test molars received laser and fluoride treatment, control teeth fluoride alone. 57 participants completed the 6-month, 51 the 12-month recall. Laser treated surfaces showed very slight ICDAS improvements. Control teeth showed significantly higher ICDAS increases. Differences in ICDAS-changes between the groups were statistically significant. A total of 22% of the participants developed ICDAS-3 scores on the control teeth. Microsecond short-pulsed 9.3µm CO2-laser irradiation markedly inhibits caries progression in pits and fissures in comparison to fluoride varnish alone.
11627-13
Author(s): Andrés F. Zuluaga, Excelitas Technologies Corp. (United States); René Daher, Ivo Krejci, Univ. de Genève (Switzerland)
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An optical coherence tomography (OCT) system with a prototype dental probe was used to image fresh, frozen porcine jaws to evaluate the ability to identify different tissue types in the periodontium. OCT reliably imaged structures in the tooth and the periodontium to optical depths of approximately 3 mm at the 18 swine premolar sites measured. Measurements of the cemento-enamel junction (CEJ) to alveolar crest (AC) distance with OCT were compared to microphotography measurements at matching anatomic sites. Strong correlation between the measurements was observed (Person correlation r=0.89). Bland-Altman analysis of the agreement between measurements showed good agreement, no systematic bias, and zero fixed bias (p<0.01). This pilot study suggests OCT may be used to optically, non-invasively monitor periodontal conditions, in particular changes in alveolar bone level as measured by the CEJ-AC distance, in real time.  
11627-14
Author(s): Eran Nair Mesquita de Almeida, João Felipe Besegato, Joatan Lucas de Sousa Gomes Costa, Priscila Borges Gobbo de Melo, Univ. Estadual Paulista "Júlio de Mesquita Filho" (Brazil); Vanderlei Salvador Bagnato, Instituto de Física de São Carlos, Univ. de São Paulo (Brazil); Alessandra Nara de Souza Rastelli, Univ. Estadual Paulista "Júlio de Mesquita Filho" (Brazil)
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Tooth whitening is a widely known technique, however, nowadays, whitening with a violet light source has emerged, which is a visible and non-ionizing light. It acts by physical and non-chemical mechanisms, allowing the breakdown of pigment molecules in the teeth. The technique is performed in-office and can be associated with the use of transparent whitening gel based on carbamide peroxide (PC) or hydrogen peroxide (PH). To evaluate a whitening procedure, it is necessary to consider its action on the surface and microhardness of dental enamel and the ability to not alter the mineral content.
11627-15
Author(s): Nhan Le, Univ. of Washington (United States); Hrebesh M. Subhash, Latonya K. Liverman, Colgate-Palmolive Co. (United States); Ruikang Wang, Univ. of Washington (United States)
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A reversible gingivitis case was recorded using fluorescence-integrated optical coherence tomography (Fi-OCT). Gingival inflammation was observed between tooth #24 and #25, or ISO #31 and #41 . The OCT and its functional angiography (OCTA) results show that these tools can be used to track the changes in the structure and angiogenic properties of the gingiva during gingivitis and its resolution. The fluorescence results showed a reduction of red fluorescence signal (produced by mature biofilm) from Day02 to Day06. The observed gingival inflammation appeared to fully resolve 6 - 8 days after the peak inflammation. We conclude that the progression of reversible gingivitis can be detected in-vivo at micron-resolution (25μm at focus) using OCT/OCTA. We also demonstrate that the biofilm-mediated inflammatory trigger can be monitored using fluorescence imaging.
11627-16
Author(s): Manuja Sharma, Lauren K. Lee, Matthew D. Carson, David S. Park, Se Won An, Micah G. Bovenkamp, Jess J. Cayetano, Ian A. Berude, Zheng Xu, Alireza Sadr, Shwetak N. Patel, Eric J. Seibel, Univ. of Washington (United States)
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Measuring the activity of oral-bacteria in plaque, the sticky biofilm on enamel, can provide the presently lacking oral feedback to patients. We have developed O-pH, optical pH monitor that measures pH in the range of 4-7.5 and tested in vivo on 25 pediatric subjects (10-18 years) with high caries risk, 18 of these subjects had a dental cleaning within last two months and 7 didn’t have a cleaning in over 2 months. The average pH after a sugar rinse from regions of biofilm associated with caries was 5.8 and 0.5 units lower than the biofilm of sound enamel.
11627-17
Author(s): Caroline Tonon, Massachusetts General Hospital (United States), Harvard Univ. (United States); Beatriz Panariello, Indiana Univ. (United States); Denise Spolidorio, Univ. Estadual Paulista "Júlio de Mesquita Filho" (Brazil); Marlus Chorilli, Univ. Estadual Paulista "Júlio de Mesquita Filho" (Brazil); Simone Duarte, Indiana Univ. (United States)
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The aims of this study were to synthesize polymeric nanoparticles loaded with Curcumin (Cur-NP) and to evaluate their effect as photosensitizers (PS). Cur-NP was synthesized using polycaprolactone as a polymer and characterized by physicochemical properties and encapsulation efficiency (EE). For the antibiofilm evaluation, the activation of Cur-NP and Cur by blue light were compared using biofilms models of Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus oralis formed on titanium specimens. Data were analyzed by one-way ANOVA with Tukey post-hoc test (p <0.05). The properties of Cur-NP showed small particle size, being homogeneous and stable in solution, with an EE of 67.5% Cur. The results showed Cur-NP and Cur antibiofilm activity at 500 and 250 µg/mL concentration when photoactivated by blue light, as confirmed by the CLSM images. Both Cur and Cur-NP are promising PS which can be used in PACT against peri-implant diseases.
11627-18
Author(s): Jacob SImon, Emmanuel O. Mogire, Sam Y. Yun, Univ. of California, San Francisco (United States); Daniel Fried, Univ of California San Francisco (United States)
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Intraoral imaging of teeth with near-IR light provides increased contrast of dental caries and restorative materials compared to visible inspection and digital radiography. The objective of this study was to investigate the near-IR optical properties of the dental pulp-chamber floor, walls and canal orifices. We imaged in vitro extracted human posterior teeth at 1300-nm and 1500-1700-nm in reflectance and transillumination and compared the tissues properties with visible light images and quantitative light fluorescence. Transillumination of posterior teeth at both 1300-nm and 1500-1700-nm yielded significantly higher contrast between the pulp-chamber floor and walls than all other methods.
11627-19
Author(s): Marwa Abdelaziz, Daniel Fried, Nai-Yuan N. Chang, V. Yang, William A. Fried, Jong Seto, C. Darling, Univ. of California, San Francisco (United States)
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This study investigated the utility of using OCT to monitor SDF application over time. Twenty dentin blocks each with 5 windows were exposed to a demineralization solution to produce carious lesions. Treatment windows included sound, sound+SDF, lesion, lesion+SDF, lesion+SDF+SDF. Lesion depth, mean reflectivity over the lesion depth and optical penetration through the lesions were monitored with OCT for 12 weeks. OCT was able to show changes in the reflectivity and optical penetration in demineralized and sound dentin after SDF application over time. Such changes can potentially be monitored to determine if and when re-application of SDF is needed.
11627-20
Author(s): Yihua Zhu, jacob Simon, Chung Ng, Daniel Fried, Univ of California San Francisco (United States)
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We have developed a clinical probe capable of acquiring simultaneous short wavelength infrared (SWIR) cross-polarized reflectance and occlusal transillumination images of lesions on tooth proximal and occlusal surfaces. We hypothesize that the dual SWIR reflectance and transillumination probe will improve the diagnostic accuracy of the device by reducing false positives since it is unlikely that confounding structural features or specular reflection are going to be present in both reflectance and transillumination images. In addition, the dual probe will provide complementary diagnostic information about lesion severity to help discriminate early superficial lesions on tooth surfaces from deeply penetrating lesions. The dual probe was 3D printed and equipped with a compact InGaAs camera and broadband superluminescent diode light sources that emit broadband light at 1300 nm for occlusal transillumination and 1600 nm light for cross-polarization reflectance measurements.
Session PS: Poster Session
11627-21
Author(s): Hao Liu, Nanjing Univ. of Science and Technology (China); Nick Chang, Univ. of California, San Francisco (United States); Wanrong Gao, Nanjing Univ. of Science and Technology (China); Daniel Fried, Univ of California San Francisco (United States)
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The purpose of this study was to test the hypothesis that the activity of arrested caries lesions on the coronal surfaces of extracted teeth would be changed by reducing the thickness of the highly mineralized transparent surface layer, which was measured using polarization sensitive optical coherence tomography (PS-OCT). The lesion activity was assessed using SWIR reflectance and thermal imaging during forced air drying of the lesion before and after mechanical removal of a surface layer ~ 50-µm thick covering the lesion. Both the intensity change in SWIR reflectance images at 1500-1750-nm wavelengths after drying the lesions and the change in thermal emission measured with a thermal camera at 8-13-µm wavelengths increased significantly (P<0.05) after reducing the thickness of the mineralized surface layer in the lesions indicating the permeability of the lesion to fluids increased.
11627-22
Author(s): John Tressel, Marwa Abdelaziz, Daniel Fried, Univ. of California, San Francisco (United States)
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Changes in the reflectivity of lesions on the proximal surfaces of extracted human teeth were measured at 1500-2340-nm and at 1950-nm as they were dried with air. An extended range tungsten-halogen lamp with a long pass filter (1500-2340-nm) and a broadband ASE source centered near the peak of the water-absorption band at 1950-nm were used as light sources and an extended range InGaAs camera (1000-2340-nm) was used to acquire reflected light images as the samples were dried with air. SWIR light at 1950-nm yields extremely high contrast of demineralization and appears to be the optimum wavelength for the assessment of lesion activity on tooth coronal surfaces.
11627-23
Author(s): Filipp Kashirtsev, Jacob C. Simon, Daniel Fried, Univ. of California, San Francisco (United States)
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Dental fluorosis is an increasing problem in the U.S. due to the widespread use of fluoride to reduce dental caries. Fluorosis causes hypomineralization of the enamel during tooth development and mild fluorosis is visible as faint white lines on the tooth surface while the most severe fluorosis can result in pitted surfaces. It is difficult to quantify the severity of fluorosis and assessments are limited to subjective visual assessments. Dental fluorosis appears with very high contrast at short wavelength infrared (SWIR) wavelengths beyond 1400-nm and we hypothesize that these wavelengths may be better suited for detecting mild fluorosis and for estimating the severity. In this study the contrast of fluorosis of varying severity on extracted human permanent teeth was measured at SWIR wavelengths ranging from 1300-2000-nm using an extended range InGaAs camera and broad band light sources. Optical coherence tomography was used to measure the depth of hypomineralization.
Conference Chair
Univ. of California, San Francisco (United States)
Conference Chair
Univ. of California, San Francisco (United States)
Program Committee
IPG Medical Corp. (United States)
Program Committee
Charles Univ. in Prague (Czech Republic)
Program Committee
Univ. Stuttgart (Germany)
Program Committee
Bio-Medical Consultants, Inc. (United States)
Program Committee
Universitätsklinikum Bonn (Germany)
Program Committee
Univ. of Washington (United States)