During the last 1.5 decades there have been significant scientific advances and technology developments in diagnosis, treatment and health care using light. Optical methods are generally noninvasive and sensitive to unique tissue contrast (absorption, scattering, birefringence, fluorescence, and nonlinear signal generation etc.) that complements other biomedical diagnostic technologies. Optical-based instruments/devices can be extremely compact and cost effective, suitable for home, clinic and global health care. The field of biomedical photonics is highly multi-disciplinary and has been attracting more and more researchers from physics, chemistry, electrical engineering, biomedical engineering, computer science, nanotechnology and many other disciplines. The past few years have also witnessed the integration of biomedical photonics with other technologies, such as photo-acoustics, optics-molecular and nano-techniques, optics and MRI, optics and positron emission tomography (PET), and more recently with artificial intelligence. Clinical applications of emerging biomedical photonics technologies span many areas including ophthalmology, dermatology, cardiology, gastroenterology, dentistry, urology, and neurology, etc. The objective of this conference is to provide a technical forum for engineers, scientists, clinicians, and health care industry and government agencies to report, share and learn the latest fundamental, translational, and clinical research and developments in the field of biomedical optics, and promote cross-disciplinary, institutional, and international collaborations. The topics of this conference are broad and will cover (but are not limited to) the following: ;
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Conference 11900

Optics in Health Care and Biomedical Optics XI

In person: 10 - 12 October 2021
On demand starting 10 October 2021
View Session ∨
  • Opening Ceremony and Plenary Session I
  • 1: Novel Microscopy I
  • Plenary Session II
  • 2: Novel Microscopy II
  • 3: Advanced Optical Technologies I
  • 4: Advanced Optical Technologies II
  • Poster Session
  • 5: OCT and Applications
  • 6: Biomedical Spectroscopy
  • 7: Translational Optical Techniques for Clinical Medicine I
  • 8: Translational Optical Techniques for Clinical Medicine II
  • 9: Nanobiophotonics
  • 10: Photoacoustic Imaging
2021-09-27T23:47:23-07:00
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UPCOMING LIVE EVENTS:
Opening Ceremony and Plenary Session I
In person / Livestream: 10 October 2021 • 09:00 - 12:00 China Standard Time
9:00: Opening Ceremony
9:20: Awards and Recognition

11900-501
Author(s): Rebecca R. Richards-Kortum, Director, Rice 360 Institute for Global Health Technologies (United States), Rice Univ. (United States)
In person / Livestream: 10 October 2021 • 09:30 - 10:10 China Standard Time
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This talk will examine the challenges of designing and translating new low-cost optical technologies to improve global health equity, drawing from examples to improve newborn survival in African hospitals, to improve early detection of cervical cancer for women in Texas and Latin America, and to improve point-of-care diagnosis of COVID-19. The talk will summarize lessons learned to increase the diversity of innovation teams, and to increase the impact and sustainability of the resulting innovations.
Tea/Coffee Break 10:10 - 10:40
11890-502
Author(s): Wentao Wang, State Key Lab. of High Field Laser Physics (China), CAS Ctr. for Excellence in Ultra-intense Laser Science (China), Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences (China)
In person: 10 October 2021 • 10:40 - 11:20 China Standard Time
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X-ray free-electron lasers can generate intense and coherent radiation at wavelengths down to the sub-ångström region and have become indispensable tools for applications in structural biology and chemistry, among other disciplines. Several X-ray free-electron laser facilities are in operation; however, their requirement for large, high-cost, state-of-the-art radio-frequency accelerators has led to great interest in the development of compact and economical accelerators. Laser-wakefield accelerators can sustain accelerating gradients more than three orders of magnitude higher than those of radio-frequency accelerators, and are regarded as an attractive option for driving compact X-ray free-electron lasers. However, the realization of such devices remains a challenge owing to the relatively poor quality of electron beams that are based on a laser-wakefield accelerator. After ten years of efforts, we present an experimental demonstration of undulator radiation amplification in the exponential-gain regime by using electron beams based on a laser-wakefield accelerator.
11893-503
Author(s): Rui Zhu, Peking Univ. (China)
In person: 10 October 2021 • 11:20 - 12:00 China Standard Time
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Perovskite solar cells have attracted tremendous attention in recent years due to the high device performance and the superior optoelectronic properties of perovskite materials. In this talk, I will give an introduction about the advances of perovskite solar cells. Then, I will present our efforts on improving the device performance and understanding the device physics of perovskite solar cells. In addition, I will also discuss the advantages of perovskite solar cells for space aircraft application. We have some preliminary efforts of sending the perovskite solar cells into near space. I will share our view about the exciting possibilities for perovskite solar cell technology.
Break
Lunch Break 12:00 - 13:30
Session 1: Novel Microscopy I
In person: 10 October 2021 • 13:30 - 15:00 China Standard Time
Session Chair: Ying Gu, Chinese PLA General Hospital (China)
11900-1
Author(s): Junle Qu, Shenzhen Univ. (China)
In person: 10 October 2021 • 13:30 - 14:00 China Standard Time
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We developed an adaptive optics (AO) based multifocal structured illumination microscopy (MSIM) system(AO-MSIM) for deep penetration, superresolution imaging. A spatial light modulator was used to generate a multi-focus excitation lattice, and the multi-focus structured illumination imaging is realized by stacking the phase of the grating. Based on the detected wavefront information, a spatial light modulator and a deformable mirror were used to correct the aberrations of excitation and emission paths respectively. By using pixel relocation and deconvolution image reconstruction algorithm, spatial resolution of 150nm was achieved at an imaging depth of 500μm. The applications of AO-MSIM technology in imaging thick brain tissue samples were demonstrated.
11900-3
Author(s): Tongyu Huang, Ruoyu Meng, Hui Ma, Tsinghua Univ. (China)
In person: 10 October 2021 • 14:00 - 14:15 China Standard Time
11900-4
Author(s): Jing Huang, Fudan Univ. (China); Xiaoguang Shao, Jiahua Pan, Shanghai Jiao Tong Univ. (China); Minbiao Ji, Fudan Univ. (China)
In person: 10 October 2021 • 14:15 - 14:30 China Standard Time
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Three-dimensional pathological imaging for human prostate cancer has important scientific and clinical significance. We develop new clearing methods for human prostate puncture strip and investigate the volumetric chemical information provided by stimulated Raman scattering and second harmonic generation. In particular, protein-lipid ratio, cell density, lipid cluster density, collagen fiber directions and prostate cancer stage will be analyzed.
11900-5
Author(s): Lanxin Zhu, Chengqiang Yi, Peng Fei, Huazhong Univ. of Science and Technology (China)
In person: 10 October 2021 • 14:30 - 14:45 China Standard Time
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Artifacts, nonuniform resolution and a slow reconstruction speed have limited the full capabilities of emerging light-field microscopy for in toto extraction of dynamic spatiotemporal patterns in samples. We overcome this limitation through combining a view-channel-depth (VCD) neural network with light-field microscopy, yielding artifact-free three-dimensional image sequences with uniform spatial resolution and high-video-rate reconstruction throughput. We imaged neuronal activities across moving Caenorhabditis elegans and blood flow in a beating zebrafish heart at single-cell resolution with volumetric imaging rates up to 200 Hz.
11900-10
Author(s): Jiaju Cheng, Tsinghua Univ. (China); Peng Zhang, Beijing Jiaotong Univ. (China), Key Lab. of Molecular Imaging, Institute of Automation (China); Chuangjian Cai, Yang Gao, Tsinghua Univ. (China); Jie Liu, Beijing Jiaotong Univ. (China); Hui Hui, Key Lab. of Molecular Imaging, Institute of Automation (China); Jie Tian, Key Lab. of Molecular Imaging, Institute of Automation (China), Beijing Advanced Innovation Ctr. for Big Data-Based Precision Medicine, Beihang Univ. (China); Jianwen Luo, Tsinghua Univ. (China)
In person: 10 October 2021 • 14:45 - 15:00 China Standard Time
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Utilizing reflective photons could circumvent the penetration limit of FMT, enabling reconstruction of fluorescence distribution near the surface regard less of the object size and extending its applications to surgical navigation and so on. Therefore, a time-domain reflective fluorescence molecular tomography (TD-rFMT) is proposed. The system excites and detects the emission light from the same angle within a field of view of 5 cm. Because the detected intensities of targets depend strongly on the depth, the reconstruction of targets in deep regions would be evidently affected. Therefore, a fluorescence yield reconstruction method with depth regularization and a weighted separation reconstruction strategy for lifetime are developed to enhance the performance for deep targets. Through simulations and phantom experiments, TD-rFMT is proved capable of reconstructing fluorescence distribution within a 2.5-cm depth with accurate reconstructed yield, lifetime, and target position(s).
11900-2
Author(s): Qi Zhao, Puxiang Lai, The Hong Kong Polytechnic Univ. (Hong Kong, China)
On demand starting 10 October 2021
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Light has been nearly perfect in many aspects to probe and treat biological tissues. Its applications, however, are limited to superficial layers beneath sample surface or compromised resolution at depths due to the inherent strong scattering nature of light in tissue. Since 2007, optical wavefront shaping has demonstrated its powers against such strong scattering to achieve focused or controllable optical delivery within or through scattering media. In this talk, we present our recent endeavors in this field towards robust and optimum optical focusing and stimulation. Future roadmaps are also discussed.
Break
Tea/Coffee Break 15:00 - 15:30
Plenary Session II
In person / Livestream: 10 October 2021 • 15:30 - 16:20 China Standard Time
15:30: Welcome and Introduction

Q&A period will follow after the talk
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Author(s): Peter L. Knight, Blackett Lab., Imperial College London (United Kingdom), UK National Quantum Technology Strategic Advisory Board for UKRI (United Kingdom)
In person / Livestream: 10 October 2021 • 15:35 - 16:05 China Standard Time
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The UK through a mix of government and industry funding has committed more than £1Bn over 10 years to a coordinated programme in quantum technology. Seven years into this programme I will describe here how we got there, and our goals for the future. The UK National Quantum Technology Programme has induced a step change in the UK’s capabilities for pushing forward a new sector in future information technologies. I describe how the programme arose and the activities it supported and influenced to deliver these new capabilities, building on a first phase almost £480M investment across several UK government agencies. The UK programme is now in its second phase, with a further substantial investment by UK government and global industries in the UK making a total of over £1Bn. I will describe our plans for ensuring the advanced quantum science and demonstrator platforms in imaging, sensing, communications and computing developed over the past seven years will drive the formation of the QT sector and embed quantum tech in a broad range of industries. References 1. “Blackett Review: The Quantum Age: technological opportunities.” Government Office for Science, Available: https://www.gov.uk/government/publications/quantum-technologies-blackett-review; https://uknqt.ukri.org/files/strategicintent2020/; Peter Knight and Ian Walmsley 2019 Quantum Sci. Technol. 4 040502
Break
Tea/Coffee Break 16:20 - 16:30
Session 2: Novel Microscopy II
In person: 10 October 2021 • 16:30 - 17:45 China Standard Time
Session Chair: Junle Qu, Shenzhen Univ. (China)
11900-6
Author(s): Wei Zheng, Shenzhen Institutes of Advanced Technology (China)
In person: 10 October 2021 • 16:30 - 17:00 China Standard Time
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We developed a short-wavelength two-photon excitation autofluorescence microscopy system using a home-made 520 nm femtosecond fiber laser as the excitation source. In vivo high-resolution 3D imaging of epithelial tissue was performed based on the signal of endogenous fluorophores, e.g. tryptophan and hemoglobin.
11900-7
Author(s): Rui Pu, Xin Guo, Qiuqiang Zhan, South China Normal Univ. (China)
In person: 10 October 2021 • 17:00 - 17:15 China Standard Time
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A novel emission depletion super-resolution microscopy (STED-like microscopy) by harnessing the effect of surface defects of lanthanide-doped nanoparticles was demonstrated in our work. a high-efficiency emission depletion process (> 95%) was achieved with a depletion saturation intensity of 19.4 kW/cm2, which is three orders of magnitude lower than achievable with conventional dye probes. The approach based on surface-mediated energy migration process, breaks the fundamental high-intensity constraint of STED imaging and provides background-free, contrast-enhanced images at a spatial resolution of ~25.4 nm. This strategy leads to a 24-fold improvement in spatial resolution over conventional multiphoton laser-scanning microscopy.
11900-8
Author(s): Binguo Chen, Honghui He, Hui Ma, Tsinghua Univ. (China)
In person: 10 October 2021 • 17:15 - 17:30 China Standard Time
11900-9
Author(s): Shaowei Wang, Xi'an Jiaotong Univ. (China)
In person: 10 October 2021 • 17:30 - 17:45 China Standard Time
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Recent studies have revealed the emerging roles of lipids in many metabolic diseases, including NAFLD, diabetes, obesity, and atherosclerosis. Most of lipid related research in biological laboratories relies on the detection and analysis of lipids in cells or fixed tissues, which hampers our understanding of dynamic lipid metabolism in live animals. Herein, we report an example of organic dots with high targeting specificity for in vivo labeling of lipids in live animals. The organic dots emit ultrabright three-photon fluorescence in far red/near infrared (NIR) region under NIR-II laser (1200 nm) excitation. The in vivo labeling of lipids in atherosclerotic plaques of brain vasculatures and carotid arteries were demonstrated by intravital three-photon fluorescence imaging. Our study highlights the great promise of organic multiphoton fluorophores for in vivo study of lipid dynamics in living systems.
11900-49
Author(s): E. Du, Shuhao Shen, Anqi Qiu, Nanguang Chen, National Univ. of Singapore (Singapore)
On demand starting 10 October 2021
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Laser speckle imaging has been an indispensable tool for in vivo imaging of blood flow in biological tissues. Here we report a novel design of laser speckle imaging system, which combines confocal illumination and detection with various speckle analysis methods. An illumination line is formed using a cylindrical lens and a 1-D scanning mirror is used to rapidly scan the line across the sample surface. The backscattered light is detected with a line camera at the confocal position. The acquired line speckle patterns can be analyzed with different methods, including temporal autocorrelation and spatial evaluation of speckle contrast, to retrieve the maps of correlation time and flow velocity. The line-scan configuration enables fast image acquisition, while confocal detection helps reject out-of-focus light and define a small focal volume. In vivo image experiments with chick embryos have demonstrated the excellent imaging performance, including depth selectivity, high spatial resolution
Session 3: Advanced Optical Technologies I
In person: 11 October 2021 • 08:30 - 10:00 China Standard Time
Session Chair: Dan Zhu, Wuhan National Research Ctr. for Optoelectronics (China)
11900-11
Author(s): Jiayu Li, Yuhan Cui, Zewen Wei, Tianfeng Zhou, Qin Li, Beijing Institute of Technology (China)
In person: 11 October 2021 • 08:30 - 09:00 China Standard Time
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In this work, we firstly presented an all-glass microfluidic flow cytometer (agFCM) which remarkably improved the optical performance and corresponding blood cell detection accuracy. Picosecond laser was introduced to pattern microfluidic channels, on-chip optical waveguides and on-chip micro-lens on a glass substrate (made with fused silica). The glass debris and burrs caused by laser machining were removed from microfluidic channels by ultrasonic cleaning and CO2 laser reflux respectively. The fabricated glass micro-channel with on-chip lens was sealed by bonding another glass layer to form the agFCM chip. The experimental results demonstrated that, compared with PDMS based devices, agFCM chip improved the optical performances. The performances of agFCM were verified by microsphere experiments. As expected, the improved optical parameters of agFCM resulted in related improvement on detecting accuracy.
11900-12
Author(s): Xiangwei Zhao, Southeast Univ. (China)
In person: 11 October 2021 • 09:00 - 09:30 China Standard Time
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Digital single molecules detection,such as digital PCR and ELISA, is attracting more and more attentions recently. However, the cost is still high and sophiscated instruments are needed. Here, we proposed a microparticle based digital single molecule coungting method, in which single molcules are captured by microparticles and their chemilluminescence signals are imaged by lens-free technique. This method makes digital single molecule detection compatable with point of care testing (POCT).
11900-13
Author(s): Jiawei Song, Nan Zeng, Hui Ma, Tsinghua Univ. Shenzhen International Graduate School (China)
In person: 11 October 2021 • 09:30 - 09:45 China Standard Time
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Polarimetry is sensitive to the microstructures and has its advantages in characterizing the tissue microstructures. Our previous work on dynamic polarization changes with tissue clearing has investigated the tissue variations by using glycerol, saturated sucrose and formamide. In this study, we use a fast Mueller matrix microscope to quantitatively compare the different mechanisms of two clearing agents: glycerol and formamide. We obtain the temporal Mueller matrix images and extract the temporal features of polarization parameters. Except the depolarization and linear retardance, we also analyze Mueller matrix transformation (MMT) parameters and rotation invariants. The former is used to characterize the microscale anisotropy of scattering media, and the latter is suitable for assessing the breaking of symmetries of scattering matrix. These Mueller matrix parameters convey the micro characteristic of biological tissues, and then help to explore and explain the microstructural changes
11900-14
Author(s): Jiaxing Gong, Qi Li, Hui Zhang, Jing Wang, Huazhong Univ. of Science and Technology (China)
In person: 11 October 2021 • 09:45 - 10:00 China Standard Time
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We proposed a new approach to evaluate tissue rheological properties by assessing the stochastic motion of optical vortices in the pseudo-phase representation of laser speckles. By studying the vortex MSD and VAF, we observed the anomalous diffusion of optical vortices and evaluated the viscoelastic modulus of different gel phantoms and tissue samples with diverse mechanical and optical properties. We established the direct relationship which connects the microscopic motion of the optical vortices to the macroscopic viscoelastic response of the multiple scattering viscoelastic media. We can imagine amounts of biomedical applications including blood coagulability monitoring, atherosclerotic plaque characterization, and tumor localization.
Break
Tea/Coffee Break 10:00 - 10:30
Session 4: Advanced Optical Technologies II
In person: 11 October 2021 • 10:30 - 12:00 China Standard Time
Session Chair: Buhong Li, Fujian Normal Univ. (China)
11900-15
Author(s): Dan Zhu, Huazhong Univ. of Science and Technology (China)
In person: 11 October 2021 • 10:30 - 11:00 China Standard Time
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Tissue optical clearing has shown a great potential for solving this problem. In this presentation, we developed easy-to-handle, switchable, and safe optical clearing skull windows without craniotomy. It permit us to image cortical cell structures at synaptic resolution, but also for vascular structure and function by combining with various optical imaging techniques. Through the transparent skull window, laser not only successfully opened cortical BBB, but also caused vascular obstruction. Thus, it has the potential for use in basic research on the physiological and pathologic processes of cortical vessels.
11900-16
Author(s): Xinyue Su, Tao Peng, Qin Li, Zewen Wei, Beijing Institute of Technology (China); Xuantao Su, Shandong University (China)
In person: 11 October 2021 • 11:00 - 11:15 China Standard Time
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White blood cells are a significant part of immune system, which can prevent human body from infection and invasion of foreign invaders. The count and recognition of white blood cells plays an important role in modern clinical practice. There is an urgent need to modify the conventional methods (such as cytometry), which are time-consuming and labor-intensive for white blood cell counting. This paper describes a microfluidic cytometer for white blood cells integrated a three-dimensional hydrodynamic focusing system and on-chip optical components, which can realize single-cell fluid flow and single-cell detection. Through the experiment, the device achieves the hydrodynamic focusing of cell flow and the detection of side scatter and fluorescence. For classifying and counting of white blood cells, we further perform an experiment using blood samples and get fairly good results.
11900-17
Author(s): Hui Zhang, Jiaxing Gong, Qi Li, Wenjian Lu, Yaowen Zhang, Jing Wang, Huazhong Univ. of Science and Technology (China)
In person: 11 October 2021 • 11:15 - 11:30 China Standard Time
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The whole dynamic process of blood coagulation can be characterized by tracking the MSD of optical vortices with our previous coagulation measurement system. To develop a portable prototype of the coagulation detection system, we use an embedded system for whole blood laser speckle image acquisition, and apply deep learning methods for the temporal and spatial interpolation of the acquired images and the fast localization of optical vortex. The prototype implementation with a compact optical design and experimental validation provide a feasible idea and method for the miniaturization of blood coagulation devices.
11900-18
Author(s): Yangyang Liu, Lin Meng, Nanjing Institute of Technology (China); Weitao Li, Nanjing Univ. of Aeronautics and Astronautics (China)
In person: 11 October 2021 • 11:30 - 11:45 China Standard Time
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Microwave hyperthermic tumor ablation has played an important role in the treatment of bone tumors because of its obvious curative effect, less side effects and fewer complications. However, the real-time thermal injury evaluation model of bone and related soft tissue during microwave ablation is still in exploration. In this paper, it proposes to study thermal injury evaluation optical parameters of bone tissue during microwave ablation. In the microwave ablation experiment, it studied the changes of tissue near-infrared spectrum and optical parameters (reduced scattering coefficient, slope factor, area factor) during specific microwave ablation conditions. It was found that some factors (slope(524-532) and slope(565-570)) changed significantly, correlated with microwave ablation time, which could be used as evaluation parameters of bone thermal injury. The results lay a foundation for establishment of a real-time evaluation system of bone tissue during microwave ablation.
11900-19
Author(s): Jiaqing Tao, Tianlong Chen, Chenfei Ren, Huiyun Lin, Yi Shen, Buhong Li, Fujian Normal Univ. (China)
In person: 11 October 2021 • 11:45 - 12:00 China Standard Time
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We have developed a multiple PDT parameters imaging system combining hyperspectral imaging (HSI), dual-wavelength reflection imaging (DWRI) (λ1 = 510 nm and λ2 = 660 nm) and laser speckle imaging (LSI). The capability for monitoring multi-dosimetric parameters has been demonstrated by the in vivo imaging of Hemoporfin-mediated PDT in a dorsal skinfold chamber model. The HSI allows for simultaneously monitoring the changes of Hemoporfin dosage and V-PDT induced vasoconstriction, while the DWRI and LSI were used to measure the blood oxygen saturation and blood flow velocity, respectively. This study shows that our home-built system has a promising potential for assessing the V-PDT efficiency in vivo and optimizing personalized treatment in real time.
Break
Lunch Break 12:00 - 13:30
Poster Session
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
Conference attendees are invited to attend the poster session on Monday afternoon. Come view the posters, ask questions, and network with colleagues in your field. Authors of poster papers will be present to answer questions concerning their papers. Attendees are required to wear their conference registration badges to the poster session.

Poster Setup: Monday 10:00 to 13:00
View poster presentation guidelines and set-up instructions at
https://spie.org/PA/poster-presentation-instructions
11900-22
Author(s): Lei Gao, Shanghai Institute of Technology (China), Shenzhen Institutes of Advanced Technology (China); Song Yuting, Teng Ma, Qi Zhang, Ruiming Kong, Zhuoquan Chen, Shenzhen Institutes of Advanced Technology (China); Guogang Cao, Cuixia Dai, Shanghai Institute of Technology (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Endoscope-based optical coherence tomography (OCT) had emerged as a valuable tool for high-speed volumetric imaging of subsurface features with high resolution. Here, we presented a proximal scanning endoscopic OCT technology that can enable OCTA. A nonrigid registration algorithm is used to correct the non-uniform rotational distortion (NURD). The final moving particles are generated by the spatiotemporal singular value decomposition (SVD) method to remove noise and static signals. Primary results showed that the endoscopic imaging system enabled OCTA based on 2D and 3D in vitro flow-mode experiments. The system was of great potential for advancing our understanding of histomorphology and studying the pathogenesis of pancreatic and bile duct cancers and even cardiovascular disease in clinical applications.
11900-51
Author(s): Meng Wang, Hebei Univ. of Science and Technology (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Feature extraction of pulse diagnosis signal based on Hilbert yellow transform. Shuwang Chen, Meng Wang, Yun Wang. Institute of Information Science and Engineer-ing, Hebei University of Science and Technology, Shijiazhuang,China. After denoising the measured pulse wave shape by wavelet processing, waveform analysis and feature extraction are carried out in time domain, frequency domain and time frequency domain respectively. It can be seen that the characteristics of flat pulse wave can be obtained more clearly and accurately through the combination of wavelet transform and Hilbert yellow transform, so as to facilitate the subsequent judgment.
11900-52
Author(s): Wei Qiao, Rui Jin, Tianpeng Luo, Yafeng Li, Guoqing Fan, Huazhong Univ. of Science and Technology (China); Qingming Luo, Huazhong Univ. of Science and Technology (China), Hainan Univ. (China), HUST-Suzhou Institute for Brainsmatics (China); Jing Yuan, Huazhong Univ. of Science and Technology (China), HUST-Suzhou Institute for Brainsmatics (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Optical sectioning technique is a useful tool for three-dimensional imaging of biological samples. To solve previous method constraints between imaging speed and optical section ability, we propose a single-scanning HiLo method to acquire optically sectioned images of uncleared thick tissue based on multi-line detection strategy. A series of experimental results demonstrated that our method offers a superior optical sectioning capability by integrating HiLo algorithm and virtual slit detection.
11900-53
Author(s): Mengmeng Zheng, Yamin Lin, Dan Liu, Xin Bai, Xin Zhao, Jiamin Gao, Shusen Xie, Wei Gong, Fujian Normal Univ. (China); Yun Yu, Fujian Univ. of Traditional Chinese Medicine (China); Juqiang Lin, Fujian Normal Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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A silver-based hydroxyapatite nanomaterial was developed for surface-enhanced Raman scattering (SERS) detection. The silver coated hydroxyapatite nanorods were prepared by depositing silver on the surface of hydroxyapatite nanorods using reduction method. Rhodamine 6G and adenine were used as samples to detect the enhancement effect of Raman scattering of this new nanocomposite substrate. The results show that this substrate has a significant enhancement effect for the Raman signal of Rhodamine 6G and adenine. Nano hydroxyapatite has great potential as a drug carrier to be introduced into living cells, and the enhancement of silver makes it possible to detect SERS in vivo.
11900-54
Author(s): Dan Liu, Jiamin Gao, Yamin Lin, Wei Gong, Fujian Normal Univ. (China); Xiang Wu, Qingjiang Xu, Tao Li, Fujian Provincial Hospital (China); Yun Yu, Provincial Clinical Medical College of Fujian Medical Univ. (China); Juqiang Lin, Fujian Normal Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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As one of the most common urinary diseases, the recurrence rate of kidney stones (kidney stones) is close to 50%, which has a significant impact on 5% of the world's population. This study focused on how to quickly detection renal calculi. The plasma of 10 normal volunteers and 10 patients with renal calculi were detected and analyzed by SERS technique. The PCA-LDA diagnostic algorithm was used to classify the SERS spectra of the two plasma samples. The discriminant sensitivity and specificity were 80% and 100%, respectively. Difference spectrum analysis combined with SERS band identification showed that there were observable changes between normal plasma and renal stone plasma, and the (ROC) curve of receiver working characteristics further confirmed the effectiveness of the algorithm. This study shows that the combination of SERS technology and PCA-LDA algorithm has great potential for the development of non-invasive and accurate detection of renal stone.
11900-55
Author(s): Xin Zhao, Tingting Lin, Yamin Lin, Jiamin Gao, Wei Gong, Fujian Normal Univ. (China); Xiang Wu, Fujian Provincial Hosipital (China); Yun Yu, Provincial Clinical Medical College of Fujian Medical Univ. (China); Juqiang Lin, Fujian Normal Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Surface enhanced Raman spectroscopy (SERS) of silver glue mixing plasma from patients with prostate cancer and benign prostatic hyperplasia on aluminum plates was studied. Difference spectrum analysis combined with the assignment of Raman bands showed that there was a significant difference between prostate cancer (n = 14) with benign prostatic hyperplasia (n = 14). To differentiate prostate cancer (PCa) and benign prostatic hyperplasia (BPH) further, principal component analysis and linear discriminant analysis (PCA-LDA) were used to analyze the spectra data with sensitivity of 95.8% and a specificity of 100.0%, which indicated can significantly differentiate PCa and BPH groups by using PCA-LDA. The results demonstrate that SERS combined with PCA-LDA algorithms has great potential for becoming a clinical tool for screening PCa and BPH.
11900-56
Author(s): Xin Bai, Dan Liu, Yamin Lin, Fujian Normal Univ. (China); Xiang Wu, Qingjiang Xu, Tao Li, Fujian Provincial Hospital (China); Yun Yu, Fujian Univ. of Traditional Chinese Medicine (China); Wei Gong, Juqiang Lin, Fujian Normal Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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In this study, plasma of 10 healthy volunteers and 10 patients with bladder cancer were detected by Surface-enhanced Raman spectroscopy. Difference spectrum, principal component analysis(PCA), linear discriminant analysis(LDA) algorithm and receiver operating characteristic(ROC) curve were compared and analyzed.The difference spectrum analysis shows that there are slight but significant differences in the spectra between normal plasma and bladder cancer plasma.The sensitivity and specificity of PCA-LDA algorithm are 80% and 100%, respectively.The area under the ROC curve is 0.97,which further proves the validity of the diagnostic algorithm based on the PCA-LDA diagnostic algorithm.These analyses indicate that SERS could be used as a simple and effective method for the detection of clinical cancer.
11900-57
Author(s): Jiamin Gao, Xin Bai, Yamin Lin, Dan Liu, Yating Lin, Wei Gong, Xin Zhao, Fujian Normal Univ. (China); Yun Yu, Fujian Univ. of Traditional Chinese Medicine (China); Juqiang Lin, Fujian Normal Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Prostate cancer (PCa) is the common tumor in male genitourinary system, and its early screening is crucial. In this work, we developed a rapid PCa detection technique based on surface-enhanced Raman spectroscopy (SERS). We collected the SERS spectra of serum samples from patients with PCa and healthy volunteers, then classified them by principal component analysis (PCA) combined with linear discriminant analysis (LDA). The diagnostic sensitivity and specificity were 85% and 95%, respectively. The receiver operating characteristic(ROC) curve verified the effectiveness of the diagnostic algorithm. These results show that SERS technology combined with PCA-LDA algorithm has the potential of detection and screening of PCa.
11900-58
Author(s): Zhong Ren, Tao Liu, Guodong Liu, Jiangxi Science and Technology Normal Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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To accurately distinguish the true blood from the fake blood, the near infrared spectroscopy was used. The optical absorption spectra of blood samples were obtained via a Fourier transform NIR spectroscope at 4000cm-1 to 10000cm-1. The wavelet nerual network was used to train and test the blood samples. To further improve the correct rate, the particle swarm optimization was used to optimize the parameters of WNN algorithm. Under the optimal parameters, the correct rate of distinguishing true and fake blood based on WNN-PSO algorithm can be improved to 96.7%. Therefore, near infrared spectroscopy combined with WNN-PSO algorithm has the potential value in the identification of blood origin.
11900-59
Author(s): Yuhong Fang, Wei Gong, Zheng Huang, Shusen Xie, Fujian Normal Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Acute tubular necrosis after renal ischemia-reperfusion process leads to uneven distribution of uriniferous tubules. The renal activity could be evaluated by analyzing the uniformity of renal tubules. Optical coherence tomography has been proved to have the ability of imaging the microstructure of kidney. This paper applied fractal box dimension to assess the uniformity of renal tubular distribution in OCT images of kidney. Kidneys of three Wistar rats were ischemia for 75 minutes and reperfused for one hour and a half. The normal kidney before ischemia and every 5 minutes after blood reperfusion were imaged using a SDOCT system during the ischemia-reperfusion process, The box dimensions of en face OCT images at the same depth below the renal capsule were calculated using Fractal box count toolbox in ImageJ. Results showed that the renal tubular uniformity featured by fractal box dimension of OCT images could be used to evaluate renal ischemia - reperfusion injury.
11900-60
Author(s): Haiyang Qi, Sumei Liu, Sunqiang Pan, Pengbing Hu, Chonghui Chen, Zhejiang Institute of Metrology (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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With the outbreak of COVID-19,masks,as the most important personal protective equipment, its necessity and importance becomes evident. Particle protective performance, as the key index of masks, the accuracy of its test result is very important. In this study, based on the high-precision photometer, the calibration method of particle protective performance testers for mask is studied, for ensuring the accuracy of particle protective performance test results to protect life and health. The protective performance is evaluated by calculating the percentage of particle concentration reduction of before and after the mask. Photometric method is a relatively mature technology to measure the concentration of particulate matter, with advantages of portability and quick response. Two photometers are used in the calibration of particle protective performance testers. In our experiment, the extended uncertainty of the calibration results are lower than 4%.
11900-61
Author(s): Yunfeng Mo, Ziye Chen, Jiewen Chen, Jianhua Mo, Soochow Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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In this study, we developed a handheld OCE system based on compression OCE with a specially designed stress sensor for loading measurement. The OCE system is built based on swept-source OCT. The stress sensor is constructed with a glass window and a metal ring which are connected with elastic material. The window serves as both OCE loading and OCT imaging window. The window will undergo a displacement during compression which can be sensed by OCT imaging and eventually can be converted into the loading. After simulation, optimization and physical calibration, we evaluated the handheld OCE system with the stress sensor on both artificial phantom and human skin. The results show that the handheld OCE system has a good potential for elasticity measurement on biological tissues in vivo.
11900-62
Author(s): Ziye Chen, Jianhua Mo, Yunfeng Mo, Jiewen Chen, Soochow Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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In this study, we aim to improve the quality of CNF segmentation and centerline extraction by using UNET++ and the new centerline extraction algorithm . The UNET++ is modified by adding skip connection to be more applicable to multi-scale information so as to improve the quality of CNF segmentation. Following CNF segmentation, a new CNF centerline extraction algorithm is developed based on pixel statistics and traditional image processing methods which can obtain smoother center line than the traditional thinning and distance variation algorithm. The results show that our method can outperform the conventional UNet++ in terms of Acc (accuracy), TPR (True Positive Rate), TNR (True Negative Rate), Dice and FDR (False Discovery Rate). The centerline extraction method can extract the centerline with less errors.
11900-63
Author(s): Na Meng, Xin Wang, Jie Jiang, Fujian Normal Univ. (China); Rui Ding, Zhen Han, Guilin Huiang Biopharmaceutical Co., Ltd. (China); Zheng Huang, Fujian Normal Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Photosensitizer is a key element of photodynamic therapy (PDT). Currently, only limited numbers of photosensitizers are available for antitumor PDT in China. YLG-1 is a newly developed second-generation chlorin-type photosensitizer. In this preliminary study, the killing effect of combination of YLG-1 and 652 nm diode laser were investigated on human nasopharyngeal carcinoma cells (CNE1) and human ovarian cancer cells (HO-8910). In vitro study suggests that YLG-1 mediated PDT activity has strong cancer cell killing effect and therefore potentials for antitumor PDT.
11900-64
Author(s): Shaojun Liu, Dongyu Li, Tingting Yu, Dan Zhu, Huazhong University of Science and Technology (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Diabetes can not only disrupt the blood brain barrier and the homeostasis of brain microenvironment, but also affect the function of immune cells. Since diabetes is a chronic disease, it is of great value to investigate the changes of various physiological indicators with the development of diabetes, while there are few relevant studies. In this work, the changes of blood brain barrier and microglial function in mice with the development of diabetes was in vivo monitored, using recently arisen skull optical clearing window with a variety of optical imaging techniques. The results showed that with the development of diabetes mellitus, the permeability of the blood brain barrier in the cortex of mice increased gradually, which further induced the morphological and functional changes of microglia. This study is expected to provide a reference for the study of diabetic complications, as well as interventional treatment and efficacy evaluation of diabetes mellitus.
11900-65
Author(s): Yunqi Li, Chinese PLA General Hospital (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
11900-66
Author(s): Xiwen Chen, Key Lab. of Optoelectronic Science and Technology for Medicine, Ministry of Education (China), Fujian Provincial Key Lab. of Photonics Technology, Fujian Normal Univ. (China); Shuang Wang, Northwest Univ. (China); Jianxin Chen, Fujian Normal Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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The spinal cord injury (SCI) is a common disease of the central nervous system. Early diagnosis and assessment of patients with SCI in favor of timely treatment, can help patients recover from illness as soon as possible. In this study, we use multiphoton microscopy (MPM) to obtain the high-resolution images of fresh, unfixed, unstained rat spinal cord specimens (normal spinal cord tissue and the tissue of SCI). Our results show that MPM has great potential to identify the characteristics of SCI including the changes in the proliferation and hypertrophy of astrocytes and bleeding area. With the development of MPM, this technique can act as an efficient tool for early diagnosis and assessment of SCI.
11900-67
Author(s): Jiamin Gao, Yamin Lin, Mengmeng Zheng, Shuzhen Tang, Fujian Normal Univ. (China); Wei Gong, Fujian Normal University (China); Xiang Wu, Qingjiang Xu, Tao Li, Fujian Provincial Hospital (China); Yun Yu, Fujian Univ. of Traditional Chinese Medicine (China); Juqiang Lin, Fujian Normal Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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The incidence rate of cancer has been rising continuously,however, there is still a lack of ideal early diagnosis methods.In this work,we developed a method of surface-enhanced Raman spectroscopy(SERS) combined with principal component analysis (PCA) and linear discriminant analysis (LDA) algorithm to detect patients with prostate cancer and liver cancer. The collected serum of cancer patients was detected by Raman spectroscopy.PCA-LDA algorithm was used to classify these serum SERS.The sensitivity and specificity were 100% and 90%.The receiver operating characteristic (ROC)curve further confirmed the effectiveness of PCA-LDA algorithm.The results show that this method may provide an auxiliary means for the diagnosis of liver cancer and prostate cancer.
11900-68
Author(s): Weijie Wu, Qian Liu, Shulian Wu, Fujian Normal Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Microvascular network distributes all around the body. Blood vessel is the main channel of tumor development and metastasis, and it is also the channel of drug therapy. So, the tumor vessel feature is very important for characterizing the tumor progress. In our study, OCT was applied on capturing the structure of normal and tumor tissue. Speckle variance algorithm was used to deduce vessel information. And then, vessel character, such as measurable diameters of lumen in blood vessels, vessels tortuosity index and vessel density of vascular between normal vessel and tumor vessel were displayed quantitatively. The result could uncover the difference between the normal and tumor vessel and will help to understand tumor angiogenesis.
11900-69
Author(s): Juqiang Lin, Xin Zhao, Tingting Lin, Jiamin Gao, Yamin Lin, Wei Gong, Fujian Normal Univ. (China); Xiang Wu, Tao Li, Qingjiang Xu, Fujian Provincial Hospital (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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In this study, a non-invasive and convenient method, which uses silver nanoparticles as the enhancement substrate to directly perform SERS analysis on patients' urine for differentiating prostate cancer from BPH, was presented. The difference spectrum and spectral peak assignment were used for preliminary analysis. In order to further distinguish BPH and PCa, multivariate statistical methods including principal component analysis (PCA) and linear discriminant analysis (LDA) were used to analyze the spectrum. And the diagnostic sensitivity and specificity were 90% and 80% respectively. The area under the curve (AUC) of receiver operating characteristic (ROC) is 0.980, which verifies the effectiveness of PCA-LDA algorithm. The result suggests that surface enhanced Raman spectroscopy of urine combined with PCA-LDA is a potential diagnostic tool for distinguishing PCa from BPH.
11900-70
Author(s): Peng Wan, Yusha Li, Jingtan Zhu, Jianyi Xu, Xiaomei Liu, Tingting Yu, Dan Zhu, Huazhong University of Science and Technology (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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The recently reported solvent-based optical clearing method FDISCO can preserve various fluorescent signals very well. However, the strict low-temperature storage condition of FDISCO is not conducive to long-time or repetitive imaging usually conducted at room temperature (RT). Therefore, it is important to solve the contradiction between fluorescence preservation and imaging condition. We develop a modified FDISCO clearing method, termed FDISCO+, to change the preservation condition from low temperature (LT) to RT. Two alternative antioxidants were screened out to effectively inhibit the peroxide generation in the clearing agent at RT, enabling robust fluorescence preservation of cleared samples. FDISCO+ achieves comparable fluorescence preservation with the original FDISCO protocol and allows long-time storage at RT, making it easier for researchers to image and preserve the samples. FDISCO+ is expected to be widely used due to its loose operation requirements.
11900-71
Author(s): Juqiang Lin, Jianshu Xu, Yating Lin, Jiamin Gao, Shuzhen Tang, Mengmeng Zheng, Yimei Huang, Fujian Normal Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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For the MCs degranulation detection in vivo and in real-time at single cell level, we here developed a secretion-sensitizing FRET probe, designated tryptase-sensitizing probe (Tryprobe). After treated with C48/80 or trypsin, activated MCs will degranulate to release inflammatory mediators, such as tryptase release, which will destroy the non-fluorescent FRET system of Tryprobe, thus causing the fluorescence recovery. Additionally, curcumin–pretreated P815 cells sensitized with C48/80 can significantly decline the level of degranulation by detecting the morphology change of cells in bright field and green fluorescent channel. We also established Intensity-Scatter correlation method for the reconstruction of degranulation to build an evaluation standard for single cell degranulation using Tryprobe system.
11900-72
Author(s): Ryan Muddiman, Kevin O'Dwyer, Zhengyuan Tang, Bryan M. Hennelly, National Univ. of Ireland, Maynooth (Ireland)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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This work presents a CARS system incorporating a super-continuum light source for multiplex addressing of the whole Raman signature of biological samples with < 50 ms acquisition time. This method is becoming the standard for high-speed spectral classification of differences between molecular functional groups and whole molecules, which has a great potential for diagnosing diseases from blood, liquid biopsies, and tissue biopsies. This work reports on our findings on the light source requirements as well as the optimization of the whole bCARS system for Raman fingerprinting of biological samples, including the collection optics, sample preparation and post-processing of the spectra.
11900-73
Author(s): Hua Liu, Luoyang Electro-optical Equipment Research Institute (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Methods on detection of objects below the surface of diffuse scattering media, particular some vivo capillaries in organs such as human beings skin and so on, using orthogonal polarized spat imaging, according to the invention comprising the steps by imaging the object in at least two different angles so that to obtain coordinates of the imaged objects, with laser in special spectral wave length. Correlative algorithms and perfect imaging methods are available to ensure this system can be corrected and optimized adequately. Results show that the system can meet the application requirements in related criteria. The determining factors are the prototype selection and the system configuration.
11900-74
Author(s): Zhi Wang, Wei Qiao, Rui Jin, Jing Yuan, Hui Gong, Huazhong Univ. of Science and Technology (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Oblique light-sheet microscopy (OLSM) is a high-throughput technology for large-scale imaging. Its imaging resolution and depth are essential but not easy to be balanced. Here, we propose a natural modulated OLSM to improve both axial resolution and imaging contrast by employing the original intensity variation along the illumination direction. We theoretically analyze the system response and experimentally verify the improvements of both resolution and optical sectioning. Combing with a vibratome, this system also demonstrates the improvement of the image quality and the reduction of data storage in large-scale tissue imaging, potentially facilitating the applications in biological visualization.
11900-75
Author(s): Kevin O'Dwyer, Katarina Domijan, Adam Dignam, Zhengyuan Tang, Marion Butler, Bryan M. Hennelly, National Univ. of Ireland, Maynooth (Ireland)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Raman micro-spectroscopy is an optical technique that can provide information on the biochemical composition of biological cells. Raman cytology, whereby Raman spectra are recorded from the nucleus of human epithelial cells is an active area of research. This typically involves the application of multivariate statistical algorithms to classify cell type, or disease group, based on the Raman spectrum. Although this approach has been shown to improve the diagnostic sensitivity of clinical cervical, bladder, and oral cytology for the identification of cancer cells, there has been no clinical adoption to date. The main reasons for this are the slow recording time and lack of reproducibility. In this paper, we report on recent work to overcome these two issues with an automated Raman cytology system based on image processing that can record several thousands of cells/day from unstained adherent epithelial cells.
11900-76
Author(s): Huihuang Deng, Haiyang Song, Huanchang Liu, Yuxin Lu, Lili Zhu, Fujian Normal Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Biological tissue is a multiple random scattering medium. A research on the propagation of acousto-optic signals in biological tissues is an important and complex problem in acousto-optic tomography (AOT). In this paper, the finite element simulation software COMSOL Multiphysics is used to simulate the propagation of acousto-optic signal modulated by ultrasound in the double-layer tissue. The effects of different types of ultrasound on the acousto-optic signals in tissues were studied. The influence of optical properties of double-layer tissue on acousto-optic signal is also discussed. The results of simulation indicate that the value of acousto-optic signal are affected by the optical properties of tissue. However the modulation depth of acousto-optic signal is only dependent on the optical and ultrasonic properties of target tissue in the ultrasonic focal volume, and It have nothing to do with the optical properties of the tissue outside the ultrasound zone.
11900-77
Author(s): Guang Han, Tiangong Univ. (China), Tianjin Univ. (China); Qianbei Guo, Siqi Chen, Hao Feng, Xinzheng Yu, Huiquan Wang, Tiangong Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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For specific cerebrovascular diseases such as epilepsy and Alzheimer's disease, it is urgent to detect the chronic changes of regional cerebral blood flow (rCBF) for a long time. interferometric diffusing wave spectroscopy (iDWS) based on optical heterodyne detection is expected to improve the detection sensitivity and reduce the interference of scalp blood flow. However, iDWS technology is difficult to achieve two-dimensional blood flow imaging. Therefore, this research proposes a interferometric diffusing speckle contrast imaging (iDSCI) system for monitoring rCBF, which combines an improved diffusion speckle contrast analysis method with iDWS technology. The dynamic phantom experiment demonstrated a good linear relationship between the reconstructed relative blood flow index and the actual flow velocity, and multi-parameter analysis validated the effectiveness of the iDSCI system in monitoring rCBF velocity, improving detection accuracy from 0.82 to 0.97.
11900-78
Author(s): Wenli Du, Xiangyu Jin, Rongxin Fu, Xue Lin, Ya Su, Han Yang, Xiaohui Shan, Wenqi Lv, Ruliang Wang, Guoliang Huang, Tsinghua Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Nucleic acid detection performs an extremely important role in the diagnosis of various diseases and the research of food and environmental safety monitoring. But its operation is often time-consuming and the implementation is difficult especially under poor environment. In this research, we report a rapid nucleic acid detection platform and realized the diagnosis of COVID-19. The platform is mainly consisted of a microchip, a motor, a CCD and can achieve automated nucleic acid detection based on the quantitating the precipitation. the detection limit of the product is 30 ng. We applied the platform to the rapid diagnosis of COVID-19. The detection result is able to be obtained in 30 minutes, and the sensitivity is 10 copies/μl. This platform is expected to be used in scenarios when rapid nucleic acid detection is needed and scenarios with insufficient conditions.
11900-79
Author(s): Yating Lin, Jianshu Xu, Jiamin Gao, Yimei Huang, Shusen Xie, Juqiang Lin, Fujian Normal Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Alcoholic liver fibrosis (ALF) is a growing global health problem, as deaths from end-stage liver cirrhosis and cancer are rising across the world. Accumulating evidence suggests that ALF is regarded as a turning point in alcoholic liver disease. Importantly, ALF is reversible and that recovery from cirrhosis may be possible. HSC activation represents a critical event in alcohol-induced fibrosis. Recent years, there is much evidence that adenosine aggravates fibrosis via the adenosine A2A receptor (A2AR) and adenosine A1 receptor (A1R), and the heteromerization of A1R and A2A R may be existed during this process. In this study, E-FRET imaging was used to detect the acetaldehyde-induced interaction between A1R and A2A R in LX-2 cells, for more comprehensively exploring, the pathogenesis of liver fibrosis in molecular scale.
11900-80
Author(s): Bowen Dong, Shanghai Institute of Technology (China); Xiangning Wang, The Sixth People's Hospital, Shanghai Jiao Tong Univ. (China); Guogang Cao, Lei Gao, Fengxian Du, Shanghai Institute of Technology (China); Qiang Wu, Sixth People’s Hospital, Shanghai Jiao Tong University,Shanghai (China); Cuixia Dai, Shanghai Institute of Technology (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Diabetic retinopathy (DR) is one of the major causes of blindness in adults suffering from diabetes. The extension technique of optical coherence tomography (optical coherence tomography angiography, OCTA) provides noninvasive and dye-free method to assess 3D retinal and choroid circulations which has been used to evaluate DR ever since it was proposed. Wide-field OCTA (WF-OCTA) has proved to be more efficient than fundus photographs and demonstrated great promise and strengths in evaluating DR, which substituted for fluorescein angiography in most cases. A convolutional neural network (CNN) was proposed to analyze OCTA images and automatically distinguish between no DR, mild non-proliferative diabetic retinopathy (NPDR), moderate to severe NPDR, and proliferative diabetic retinopathy (PDR). This technology enables early diagnosis and objective tracking of disease progression, which may help diabetic patients to minimize vision
11900-81
Author(s): Xiaochen Li, Qun Hao, Yong Huang, Xiaoxu Wang, Beijing Institute of Technology (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
11900-82
Author(s): Dongyue Liu, Zhengyuan Tang, Bryan M. Hennelly, National Univ. of Ireland, Maynooth (Ireland)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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An important consideration in the application of Raman spectroscopy in the field of diagnostics is the accurate calibration of instruments such that spectra from different clinical groups can be shared for the purpose of improving the machine learning process. It may be expected that over time, even a well-calibrated system will deviate from its initial specification and even normal handling can adversely affect calibration. Therefore, frequent calibration is a necessary part of practical Raman spectroscopy. Here we investigate a novel calibration procedure for wavelength/wavenumber calibration. It based on modelling the spectrometer, such that a mathematical relationship between wavelength, wavenumber, and detector pixel can be derived. Results demonstrate the performance of this approach is superior to traditional calibration methods in several aspects.
11900-83
Author(s): Fang Zhao, Wenyang Feng, Chunyu Fang, Yao Zhou, Peng Fei, Huazhong Univ. of Science and Technology (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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The combination of the content-aware compressed sensing (CACS) strategy with dual-side confocally-scanned Bessel plane illumination enables high-throughput 3D imaging of millimeter-to-centimeter sized samples with cellular isotropic resolution. On this basis, the sequential imaging of eight whole brains (~400 mm3 each) was realized within 10 minutes through a further utilization of the multi-sample holding module. With the increasing size of optically cleared and labeled samples and the increasing demands for multi-sample analysis nowadays, such technical advances of resolution/throughput shown in our method also becomes increasingly valuable, especially for the intoto mapping of entire organs/organisms at variable scales.
11900-84
Author(s): Tianpeng Luo, Jing Yuan, Hui Gong, Huazhong Univ. of Science and Technology (China); Qingming Luo, Hainan Univ. (China); XiaoQuan Yang, Huazhong Univ. of Science and Technology (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Multifocal confocal microscopy using a microlens arrays as the objective achieves a wide field-of-view (FOV) with high resolution. However, the resolving power of this system is restricted by numerical aperture of microlens which is not too high. Here we propose a parallel confocal microscopy with high-resolution and large FOV by introducing a rely multispot and coupled with pixel reassignment. A uniform multispot generated by a spatial light modulator is aligned with microlens arrays. The developed system provides lateral resolution of ~0.83 μm while ~1.6 times resolution enhancement after pixel reassignment compared with traditional image processing. Both fluorescent beads and mouse brain slices are demonstrated.
11900-85
Author(s): Xin Bai, Dan Liu, Yamin Lin, Fujian Normal Univ. (China); Xiang Wu, Qingjiang Xu, Tao Li, Fujian Provincial Hospital (China); Yun Yu, Fujian Univ. of Traditional Chinese Medicine (China); Juqiang Lin, Fujian Normal Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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In this paper, we used a new method of surface enhanced Raman spectroscopy (SERS) to detect the urine of 15 normal volunteers and 15 patients with kidney cancer, and successfully recorded their spectra. Compared with the SERS spectra in the normal group, it was found that there were some slight but discernible changes in the SERS spectra of kidney cancer. At the same time, we also use principal component analysis (PCA) and linear discriminant analysis (LDA) to analyze and distinguish the SERS spectra of the normal and patient samples. In addition, the receiver operating characteristic (ROC) curve further proves the effectiveness of the proposed algorithm. These analyses indicate that SERS spectroscopy has great potential in unlabeled and non-invasive screening of kidney cancer.
11900-86
Author(s): Yuxuan Zhao, Peng Wang, Peng Fei, Huazhong Univ. of Science and Technology (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Dual ring selective plane illumination microscopy (DR-SPIM) can generate a multi-color low-sidelobe Bessel light-sheet by carefully design the parameters of the inner ring, which is suited to study the organelle-organelle interaction over long term. Compared to lattice light-sheet microscopy and field synthesis method, the optical path has been simplified. The isotropic divide-stages-to-process (ID) convolutional neural network trained to super resolve 3D low SNR diffraction-limited images for different structures further improves the light usage efficiency, which allows faster volumetric imaging speed and longer observation time. In conclusion, IDDR-SPIM achieves simultaneous dual-color imaging with isotropic 100 nm spatial resolution and up to 400 frames per second temporal resolution.
11900-87
Author(s): Sinead J. Barton, Zhengyuan Tang, Bryan M. Hennelly, National Univ. of Ireland, Maynooth (Ireland)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Raman spectroscopy is a popular research avenue that can require expensive equipment in order to provide the optimal results. Determining the most appropriate equipment that can deliver the required results is a time consuming process. Having a reliable method of estimating the effect that a new optical element will have on the signal to noise ratio of the spectra collected by the system would be advantageous. This paper provides methodology for using software to virtually substitute a CCD in a given Raman spectrometer and evaluating the effect that the CCD would have on the signal to noise ratio of the resulting spectra. The methodology detailed herein shows that it is possible to emulate the effect of an alternative CCD to within 6% accuracy when the signal to noise ratio of the simulated data is compared to that of an experimental dataset.
11900-88
Author(s): Huifang Liu, Sijia Wang, Jing Wang, Cuiping Yao, Zhenxi Zhang, Xi'an Jiaotong Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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We constructed a dual responsive platform (MRPL), which enables precisely controllable drug release, increase the drug solubility and bioavailability, for effective combined therapy of PDT and NKG2D-mediated immunotherapy. MRPL-SC disassembled in the stroma through the cleavage of an MMP-2 responsive peptide and released drugs loaded for NKG2D-based immunotherapy and PDT, and finally realized a significant inhibition proliferation and metastasis of melanoma cell. In this study, the synthesized MRPL was well characterized and its effect was evaluated on A375 cells. Further immune-related molecular mechanism and in vivo assessment are ongoing to provide a new strategy for melanoma integration of PDT and immune therapy.
11900-89
Author(s): Haoyu Zhai, Zhipeng Fan, Honghui He, Hui Ma, Tsinghua Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
11900-90
Author(s): Yi Liu, China Pharmaceutical Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
11900-91
Author(s): Caiyun Miao, Jia Yu, Ocean Univ. of China (China); Lingyan Kan, Qingdao Institute of Bioenergy and Bioprocess Technology (China); Huiping Liu, Ocean Univ. of China (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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In the research area of diversity and heterogeneity of marine plankton, single-cell analysis becomes a hot issue in marine microbiology research. By combining micro-optical tweezers technology with microfluidic droplet wrapping technology, we studied a microscopic optical tweezers single-cell sorting system that consists of microscope module, optical tweezers module and sorting module. Using this system, we conduct single-cell observation, monitoring, capture manipulation, separation, and extraction of microplankton in seawater sample. Using our experimental system, we are able to provide precise technical support for downstream experiments. Our method has the advantages of flexible operation, intact cells, visualization, high viability and 100% single-cell acquisition rate.
11900-92
Author(s): Yuan Luo, Xiaoyi He, Ke Ren, Chongqing Univ. of Posts and Telecommunications (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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In order to solve the problems of low recognition accuracy for motor imagery EEG signals, this paper presents a feature extraction and classification algorithm based on decision tree and CSP-SVM. Firstly, we select the fixed frequency of signals ranging from 8 to 30 Hz. Secondly, multiple spatial filters are constructed by using the one versus the rest common spatial pattern (OVR-CSP) and extract the feature vectors. Support vector machine (SVM) is employed to classify the feature vectors so that the best spatial filter is selected. We build the first branch of decision tree with the spatial filter selected and SVM. Then, OVR-CSP and SVM are used to build the branches of the decision tree repeatedly. Finally, 2005 BCI competition IIIa data set is used to validate the effect of the proposed algorithm. The results show that the highest accuracy of the proposed algorithm can reach 94.27% which proves the effectiveness of the algorithm.
11900-93
Author(s): Kaicheng Yu, Xianlin Song, Nanchang Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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This paper proposes photoacoustic microscopy based on single-pixel imaging to realize a wide-field imaging without sacrificing the stability of the system. This method is based on the principles of photoacoustic imaging and Fourier single pixel imaging. According to the principle of Fourier single-pixel imaging, any image is a weighted superposition of a sequence of cosine stripes of different spatial frequencies, and the weight coefficients of these stripes can be obtained to reconstruct the image. Therefore, a series of spatial frequency stripes are used to illuminate the sample, and the photoacoustic signal obtained by a single ultrasonic transducer is the Fourier spectral coefficient of the structure information corresponding to the spatial frequency, all the Fourier spectral coefficients are obtained, and finally the inverse Fourier transform to obtain high-resolution images.
11900-94
Author(s): Yongchao Li, Xianlin Song, Nanchang Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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In this paper, a fast synthetic aperture photoacoustic imaging simulation platform is constructed by using K-Wave combined with synthetic aperture photoacoustic microscopic imaging algorithm. K-Wave simulation toolkit is used to simulate the propagation of photoacoustic field, photoacoustic signal recording and image reconstruction. By imaging the vascular network, the fast 3D high resolution imaging capability of the synthetic aperture photoacoustic imaging simulation platform is further verified. The establishment of the simulation platform has a good guiding significance for the theoretical research of synthetic aperture photoacoustic imaging and its application in biomedicine.
11900-95
Author(s): Zihao Li, Yuli Li, Xianlin Song, Nanchang Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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In this paper, the K-wave simulation toolbox of MATLAB is used to set up the virtual photoacoustic field and collect the photoacoustic signal of blood vessel. The results show that the MATLAB virtual Compressed Sensing photoacoustic tomography simulation platform based on k-wave can achieve high quality photoacoustic tomography with less data. The superiority of Compressed Sensing theory and the high efficiency and stability of k-wave virtual platform are verified. Also, the Compressed Sensing reconstruction algorithm OMP and ROMP are compared in this paper and the result shows that the ROMP algorithm works better.
11900-96
Author(s): Ao Teng, Xianlin Song, Nanchang Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Based on the characteristics of non-diffraction and large depth of field of Bessel beam, a virtual simulation platform of photoacoustic microscopy imaging system of Bessel beam based on finite element analysis was built by using simulation software COMSOL Multiphysics. In COMSOL, the human brain tissue was irradiated by the Bessel beam. The propagation of the Bessel beam in the brain tissue was simulated by solving the diffusion equation. And the temperature changes of gray matter and blood vessel were obtained by solving the equation of biological heat transfer. This study is helpful to understand the propagation of Bessel beam in human brain and the interaction between them, and has a certain theoretical significance for the optical imaging of human brain.
11900-97
Author(s): Zhihui Li, Xianlin Song, Nanchang Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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The conventional optical-resolution photoacoustic microscope (OR-PAM) suffers from limited depth of field (DoF) since the employed focused Gaussian beam only has a narrow depth range in focus, little details in depth direction can be revealed. Here, we developed a synthetic large volumetric optical-resolution photoacoustic microscopy using morphological pyramid fusion. A self-made optical-resolution photoacoustic microscope was used to obtain source images of the same sample with different focus. Then, morphological pyramid fusion was applied to obtain fuse image. Simulation was performed to demonstrate that this method can extend the depth of field of PAM two times without the sacrifice of lateral resolution. And the in vivo imaging of the mouse cerebral vasculature with intact skull further demonstrates the feasibility of our method.
11900-98
Author(s): Rui Wang, Xianlin Song, Nanchang Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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We studied the transmission of photons in mouse brain in both with and without intact skull states using Monte Carlo method. Photon track, optical absorption density and fluence rate were used as indicators for analysis. We found that, the photon distribution without intact skull goes farther in both longitudinal and transverse directions compared with that of with intact skull. The distribution of optical absorption density and fluence rate was fusiform and rounder on the whole with intact skull. This study will provide reference and theoretical guidance for the optical imaging of mouse brain and the study of the mouse and human brain.
11900-99
Author(s): Zhuangzhuang Wang, Xianlin Song, Nanchang Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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We study the SRCNN algorithm which is widely used in the field of image super-resolution based on deep learning and apply it to solve the problem of low resolution of photoacoustic imaging. The results show that the super-resolution reconstructed mouse cerebrovascular image has better performance compared with the traditional interpolation method through three evaluation indicators of PSNR, SSIM and AVG. SRCNN also has a good effect on the subjective visual effect. We obtained good experimental results composed of more part details, better image quality and resolution. The experiment results prove the superiority of deep learning network in solving the problem of image super-resolution. The deep learning based super resolution method has a significant application value for the problem of low resolution of photoacoustic imaging. It is hoped that this research can provide reference and theoretical guidance for improving the image quality of photoacoustic imaging.
11900-100
Author(s): Xiaofei Jin, Yiran Li, Yu Feng, Roujun Zhu, Lu Qian, Weitao Li, Zhiyu Qian, Nanjing Univ. of Aeronautics and Astronautics (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Finding multi-parameter comprehensive evaluation factors to achieve real-time evaluation of therapeutic effects has become the key for precise microwave ablation (MWA). The optical parameters (absorption coefficient (μa), reduced scattering coefficient (μ_s^'), etc.) of biological tissues also change dynamically due to changes in cell morphology and protein tertiary structure during tissue thermal damage. Real-time measurement of the optical parameters of tumor during MWA can be achieved by using minimally invasive function near-infrared spectroscopy. MWA of tumor is essentially a process of protein denaturation and gradual coagulation. During the process of coagulation, the tissue hardness (which could be reflected by Young's modulus, E) also changes in real time. Shear wave elastography (SWE) can measure Young's modulus in real-time and obtain 2D image. This paper focuses on the real-time evaluation of MWA based on reduced scattering coefficient and Young's modulus.
11900-101
Author(s): Xiangyu Jin, Rongxin Fu, Wenli Du, Xiaohui Shan, Anni Deng, Zeyin Mao, Ya Su, Xue Lin, Han Yang, Wenqi Lv, Guoliang Huang, Tsinghua Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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This program proposed an isothermal, label-free and fast nucleic acid detection system, named HiDA. HiDA system combined hyper-spectral interference signal analysis, reverse transcription, and solid-phase DNA amplification together, to realize fast and sensitive detection of SARS-CoV-2’s nucleic acids within 30 min in a label-free manner. The ORF1ab and N genes of SARS-CoV-2 could be detected at 1 copies/μL level, which was 10-times more sensitive than that of reverse transcription-PCR method. HiDA was also used to detect SARS-CoV-2 pseudovirus, 1000 copies/μL pseudovirus could be detected without the purification of nucleic acids. In conclusion, HiDA could be a potential useful system to help the diagnosis of COVID-19.
11900-102
Author(s): Zhe Li, Minnan Jiang, Jinchao Feng, Kebin Jia, Beijing Univ. of Technology (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Diffuse correlation spectroscopy (DCS) derives blood flow index (BFI) by measuring the temporal intensity fluctuations of multiply scattered light. Blood flow index (BFI) and especially its variations were demonstrated to be approximately proportional to absolute blood flow. In this paper, we have proposed a predictive method for calculating blood flow as well as oxygen saturation, based on a deep neural network of long short-term memory (LSTM) architecture. The simulated multiwavelength normalized intensity autocorrelation function data for various blood flows and oxygen saturations were used to train the LSTM architecture. The results validated the feasibility of the proposed method for quantification of blood flow and oxygen saturation simultaneously in DCS. The proposed approach would be an alternative method for oxygen metabolism monitoring.
11900-103
Author(s): Fengxian Du, Wei Qin, Lei Gao, Bowen Dong, Shanghai Institute of Technology (China); Sisi Chen, Meixiao Shen, Wenzhou Medical Univ. (China); Cuixia Dai, Shanghai Institute of Technology (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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We built a dual-wavelength (532nm and 1064nm) PAM and SD-OCT system, and PEG-GNR were used as contrast agent for in vivo imaging of rat choroid. Since the light absorption coefficient of hemoglobin is greatly reduced in the NIR spectral range, it is necessary to use exogenous contrast agent with a balanced scattering and absorption ratio. Experiments have proved that PAM in the near-infrared band with PEG-GNR can obtain choroidal microvasculature information, which verifies the feasibility of in vivo imaging. This imaging technique of contrast-enhanced NIR-PAM and OCT is a promising tool for the diagnosis of choroidal diseases in the future.
11900-104
Author(s): Xueqing Ding, Rui Qiu, Fengxian Du, Tianyang Zhao, Shanghai Institute of Technology (China); Yilei Shao, Meixiao Shen, Wenzhou Medical Univ. (China); Qiang Wu, Shanghai Jiao Tong Univ. (China); Cuixia Dai, Shanghai Institute of Technology (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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In this research, in order to investigate the mechanism of myopia, the biological parameters of the center and periphery of mouse eyes were performed by combining OCT and ZEMAX ray tracing technology. The biological parameters of myopic model mouse eyes, normal control mouse eyes and atropine-treated eyes were compared. The significant correlations of these biological parameters were analyzed, and the main factors caused myopia were obtained. This study provides data support for the study of the mechanism of myopia.
11900-105
Author(s): Zhaoqing Wu, Meijie Qi, Xidian Univ. (China); Runze Li, State Key Lab. of Transient Optics and Photonics (China); Peng Gao, Lixin Liu, Xidian Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Fluorescence lifetime is sensitive to the microenvironment of the fluorophore, and it can provide biological functional information complementary to fluorescence spectroscopy. Fluorescence lifetime measurement system is usually established based on laser scanning confocal microscopy (LSCM) that has the advantages of high resolution, optical sectioning and fast imaging speed. In this paper, a dual-color confocal fluorescence lifetime measurement system was designed and built, which employs two lasers to excite two kinds of fluorophores simultaneously, and then measure their fluorescence lifetime using two-channel time-correlated single-photon counting (TCSPC). The fluorescence lifetime measurement experiments were carried out based on the constructed microscopy system to obtain the fluorescence decay curves of eGFP (labeled to the ligand DKK1) and mCherry (labeled to the receptor LRP6).
11900-106
Author(s): Liuye Yao, Zhiyu Qian, Lu Zhou, Yu Zhang, Nanjing Univ. of Aeronautics and Astronautics (China); Yangyang Liu, Nanjing Institute of Technology (China); Weiyao Li, Lidong Xing, Nanjing Univ. of Aeronautics and Astronautics (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Photobiomodulation (PBM) is a treatment method, in that irradiation with certain wavelengths of red or near-infrared (NIR) light has been shown to produce a range of physiological effects in cells, tissues, animals and humans.Parameters like wavelength, energy density, power density and the pulse structure may influence the PBM outcomes as well. For now, there is no consistent results on effects of these factors on brain activitiesin clinical applications. In this study, we are going to explore the flicker frequency of NIR light whether and how to affect the brain by analyzing EEG signals.
11900-107
Author(s): Zhen Li, Yulan Liu, Yu Yi, Xiahui Han, Jianxin Chen, Lianhuang Li, Fujian Normal Univ. (China); Yelin Wu, Tongji Univ. Cancer Ctr., Shanghai Tenth People’s Hospital (China); Liqin Zheng, Fujian Normal Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Psoriasis is a common chronic inflammatory skin disease with high prevalence, chronicity, disfiguration, disability. Real-time detection of psoriatic pathological characteristics by optical technology is of great significance for the diagnosis and treatment of psoriasis. We used multiphoton microscopy (MPM) imaging technology based on intrinsic nonlinear optical signals to image the skin of a mouse psoriasis model induced by imiquimod (IMQ). The changes of cells and collagen in psoriasis skin tissue were obtained and analyzed, comparing with the hematoxylin and eosin (H&E) stained image. We found that the MPM technique could clearly observe the differences between normal and psoriasis skin. This is of great significance to the pathogenesis of psoriasis and also provides adjuvant imaging method for the treatment of psoriasis.
11900-108
Author(s): Zhihan Zou, Zhiyu Qian, Roujun Zhu, Nanjing Univ. of Aeronautics and Astronautics (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Currently, the effectiveness evaluation of microwave ablation is mostly performed by monitoring temperature parameters. However, the microscopic changes such as protein denaturation and the structure damage of cell cannot be completely reflected after the tissue is thermally damaged during the ablation process. Based on the reduced scattering coefficient, this thesis explored the changes in optical characteristics of biological tissues caused by the ablation process. The experimental results demonstrated that there is a significant correlation between the reduced scattering coefficient and the thermal damage process.
11900-109
Author(s): Hanqing Duan, Handi Deng, Jianpan Gao, Tsinghua Univ. (China); Tengfei Yu, Beijing Tiantan Hospital, Capital Medical Univ. (China); Yizhou Bai, Bin Luo, Beijing Tsinghua Changgung Hospital (China); Cheng Ma, Tsinghua Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Photoacoustic imaging (PAI) is an emerging image modality that can provide structural, functional, and molecular information. Superb microvascular imaging (SMI) is a novel angiography, which provides a visualization of low-speed and small-diameter blood vessel flow. To date, there is no work to compare PAI- and SMI-angiography. In this paper, we provide our recent evaluation results in terms of imaging depths, speeds, sensitivities, and resolutions of these two modalities through phantom experiments and in-vivo studies. We used PAI and SMI to image the human thyroid, and our preliminary data shows that PAI is superior in imaging speeds, and sensitivities, while SMI shows a better imaging depths . We acknowledge that more work needs to be done to compare the two techniques in diverse clinical applications more quantitatively, and we hope our work can pave the way for such more systematic studies.
11900-110
Author(s): Yuanhang Wang, Yang Bu, Shanghai Institute of Optics and Fine Mechanics (China), Univ. of Chinese Academy of Sciences (China); Yachao Cai, Shanghai Univ. (China); Xiangzhao Wang, Shanghai Institute of Optics and Fine Mechanics (China), Univ. of Chinese Academy of Sciences (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Laser induced breakdown spectroscopy (LIBS) is a rapid and simple detection method with almost no sample preparation. In this paper, LIBS was used to detect the electrolyte elements Na and K in human blood. The liquid drop method and evaporation method were used to improve the LIBS detection capability. The polynomial fitting method and wavelet transform method were proposed to optimize the LIBS spectrum. The results showed that, the signal to background ratio (SBR) of Na Ⅰ 588.99nm spectral line increased by 20.57 times after use the methods, and the SBR of K Ⅰ 766.49nm increased 24.65 times. The intensity of Na Ⅰ 588.99nm spectral line obtained by liquid drop method is 1.05 times higher than evaporation method, and 194.08 times higher than direct detection the human blood through test tube. The intensity of K Ⅰ 766.49nm spectral line obtained by liquid drop method is 2.41 times high than evaporation method. According to the results, LIBS is suitable for the detection of electrolyte e
11900-111
Author(s): Jiatian Li, Shulian Wu, Fujian Normal Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Photothermal therapy of tumors has become an important method for tumor treatment. Tumor cells are damaged caused by heat generated through photothermal effect. The outcome of biological response is mainly determined by the temperature generated during the process of photothermal therapy. So the temperature control is very important in corresponding tissues during the treatment process. In our study, the mouse skin was chosen as the research object. Thermocouple and photoacoustic sensor were combined to monitor the photothermal therapy in real time in vivo. Temperature, and photoacoustic signal were obtained during the photothermal therapy process. The results will provide effective guidance for the photothermal therapy of tissue.
11900-112
Author(s): Lu Qian, Jinzhe Zhao, Xiaofei Jin, Zhiyu Qian, Chunxiao Chen, Nanjing Univ. of Aeronautics and Astronautics (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Background and objective: The therapeutic effect of microwave ablation (MWA) precision treatment is highly related to intraoperative efficacy monitoring and postoperative curative effect evaluation. So finding an effective monitoring and evaluation factor for microwave ablation is becoming a research focus. Previous MWA experiments on in vitro porcine liver found that reduced scattering coefficient (μ_s^') is an efficient optical evaluation parameter. Results indicated that μ_s^' of normal tissue and coagulation tissue is 2–6 cm-1 and 17–19 cm-1 , respectively, and μ_s^' is highly related to the degree of thermal damage. This paper aims to validate if these results from in vitro porcine liver also applies to real tumor.
11900-113
Author(s): Yu Yi, Zhen Li, Yulan Liu, Zhijun Li, Fujian Normal Univ. (China); Zhonghua Han, Deyong Kang, Fujian Medical Univ. Union Hospital (China); Gangqin Xi, Jiajia He, Lianhuang Li, Liqin Zheng, Jianxin Chen, Jianhua Chen, Fujian Normal Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Necrosis is a form of cell death which is histologically characterized by homogeneous clusters and sheets of dead cells. Although several studies have indicated that the presence of tumor necrosis in pathological specimens may provide adverse prognostic information in solid tumor, the mechanism of necrosis is still unclear. Based on two-photon excited fluorescence (TPEF) and second -harmonic generation (SHG), multiphoton microscopy (MPM) is commonly used to monitor the morphological changes of biological tissues. In this study, we performed MPM imaging of the breast tissue and found that MPM can be used to rapidly classify the early and late-stage tumor necrosis in invasive breast cancer according to the changes of intracellular proteins. It demonstrated that MPM may provide a new assistant tool for pathologists to quickly and effectively classify the early and late-stage tumor necrosis.
11900-114
Author(s): Hongzhi Zuo, Manxiu Cui, Tsinghua Univ. (China); Bangyan Wang, Univ. of California, Santa Barbara (United States); Cheng Ma, Tsinghua Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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In PACT, the wavelength of the illumination light can be tuned to acquire multi-spectral images, such spectroscopic measurement can then be used to determine absolute or relative concentrations of endogenous chromophores and/or externally administered contrast agents.However, unlike conventional multispectral or hyperspectral imaging in which the excitation spectra are known, unmixing multispectral PA images is difficult because the excitation spectra in deep tissue are contaminated by light absorption and scattering in a highly unpredictable manner. Here, by using an analytical implementation of the Monte Carlo technique, we propose a spectral-domain model-based method to overcome this problem. Using this method, accurate estimation of blood oxygen saturation and identification of molecular probe were simultaneously realized numerically.
11900-115
Author(s): Yulan Liu, Yu Yi, Zhen Li, Zhenlin Zhan, Lianhuang Li, Fujian Normal Univ. (China); Liqin Zheng, Fujian Normal Univ (China); Jianxin Chen, Fujian Normal Univ. (China); Deyong Kang, Fujian Medical Univ. Union Hospital (China); Xiahui Han, Fujian Normal Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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The tumor microenvironment is now recognized as an important participant of tumor progression. As the most abundant extracellular matrix component in tumor microenvironment, collagen plays an important role in tumor development. The imaging study of collagen morphological characteristics in tumor microenvironment is of great significance for understanding the state of tumor. Multiphoton microscopy (MPM) based on second harmonic generation (SHG) and two-photon excitation fluorescence (TPEF) can be used to monitor the morphological changes of biological tissues without labeling. In this study, MPM was used to perform label-free imaging of the tumor border, the transition zone near the tumor, and the normal tissue away from the tumor sequentially from the center of the tumor in early invasive breast cancer samples. We found that collagen morphology varies significantly in different regions of breast cancer tumor tissue. The collagen content was further quantified and the results showed th
11900-116
Author(s): Guoqing Fan, Chenyu Jiang, Hui Gong, Jing Yuan, Huazhong Univ. of Science and Technology (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Mesoscopic imaging of a whole kidney is crucial to the disease staging and etiology exploration of hydronephrosis. However, traditional imaging methods cannot balance both macroscopic range and mesoscopic resolution. Here, we propose to acquire 3D morphology of frozen mice kidneys with a cellular resolution by autofluorescence imaging using cryo-micro-optical sectioning tomography (cryo-MOST). We imaged and compared whole acute obstructive hydronephrosis mice kidneys and their controls by cryo-MOST. The results indicate that cryo-MOST potentially facilitates to obtain three-dimensional morphology of whole hollow organs and the distribution of body fluids in them with the smallest possible deformation.
11900-117
Author(s): Huijun Wang, Shanghai Jiao Tong Univ. (China); Cuixia Dai, Fengxian Du, Bowen Dong, Shanghai Institute of Technology (China); Xinyu Chai, Shanghai Jiao Tong Univ. (China); Chuanqing Zhou, Shanghai Jiao Tong Univ. (China), Shenzhen Bay Lab. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Pulse wave velocity (PWV) is an important indicators of human arteriosclerosis. Measuring the PWV of retinal arteries can better detect early vascular-related diseases. However, the measurement results of retinal PWV from multiple research groups have a large difference in magnitude. In this study, we proposed a dual-beam variable-spacing scanning imaging method based on spectral-domain optical coherence tomography (SD-OCT). By changing the driving voltage of scanning galvanometer, the distance between two beams can be changed arbitrarily. The Doppler OCT was used to analyze the pulse waveform and propagation velocity of the fundus vessels. Based on the proposed method, we can assess the degree of fundus arteriosclerosis in human eyes more reliably.
11900-118
Author(s): Xiaoman Zhang, Huaqin Wu, Biying Yu, Shulian Wu, Jianyong Cai, Hui Li, Fujian Normal Univ. (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Continuous and non-invasive real-time measurement of human blood pressure is of great importance for health care and clinical diagnosis. Photoacoustic imaging allows absorption-based high-resolution spectroscopy in vivo imaging with a depth beyond that of optical microscopy. In this study, a novel photoacoustic imaging system is used for monitoring and imaging of vessel pulsation, which can realize simple, non-invasive and continuous measurement and recognition of blood pressure. Combined with deep learning method, a model is established to effectively evaluate the dependence of blood vessel elasticity on the blood pressure. These results can quickly and accurately identify the photoacoustic signals of blood vessels under different pressures.
11900-119
Author(s): Yidi Liu, Medical School of Chinese PLA (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Skin vascular lesions such as port wine stains (PWS) lead to facial and psychological problems, which require careful and precise treatments. The key point of treating PWS is to selectively destroy the abnormal blood vessels. Vascular-targeted photodynamic therapy (V-PDT) now is referred as the mainstream treatment, which selectively lead to the closure of the dilated vessels. However, there are still some patients can not achieve total clearance and some lesions show no response to V-PDT. The lesions consist of ectatic vessels in the superficial vascular plexus of the dermis, the inhomogeneity and complexity of them contribute to the difference in the response. This review will address what is presently known about lesion-specific characteristics of V-PDT recalcitrant PWS as well as discuss current non-invasive technologies and methods on obtaining in vivo individual PWS anatomy.
11900-120
Author(s): Bo Chen, Xuecong Feng, North China Univ. of Science and Technology (China)
In person: 11 October 2021 • 13:00 - 14:30 China Standard Time
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Coronary heart disease, as a major disease with high incidence rate, has increased the intensity of work of the radiology doctor.Using artificial intelligence technology to identify the plaque in coronary artery and judge the degree of coronary artery stenosis can effectively reduce the work intensity of CT imaging doctors.This paper studies the recognition and segmentation of coronary artery based on u-net network with attention mechanism.The u-net network with attention mechanism is trained. The loss function, accuracy and dice index were 0.0213, 0.9626 and 0.9999 respectively.Through the prediction results, we can see that the image segmentation is accurate. It provides a good foundation for the qualitative analysis of plaque and the calculation of calcification score.
11900-50
Author(s): Zhi Wang, Ragini Singh, Bingyuan Zhang, Santosh Kumar, Liaocheng Univ. (China)
On demand starting 10 October 2021
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In this work, a simple and effective sensor using single-mode fiber (SMF) tapered structure is developed to detect different concentrations of acetylcholine solutions, and its function is to test the probe's performance. A layer of synthesized gold nanoparticles (AuNPs) and zinc oxide nanoparticles (ZnO-NPs) is used to immobilize this type of SMF-based tapered structure. The work is based on the well-known phenomenon of localized surface plasmon resonance (LSPR) principle. A tapered region of the probe with a high fraction power of evanescent wave can stimulate LSPR and produce specific absorption peaks sensitive to the refractive index variation. The sensor probes performance was examined, including their stability, repeatability, reusability, and selectivity. Furthermore, the biosensor's ability to improve performance was tested in the experiment.
11900-121
Author(s): Samaria Nevarez Diaz, Orly Yadid-Pecht, Varun Vij, Raymond Turner, Univ. of Calgary (Canada)
On demand starting 10 October 2021
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An efficient method to bind Black Hole Quencher (BHQ) to gliadin from gluten has been developed. In the present work, we used the physical phenomenon of fluorescence resonance energy transfer (FRET) to study the quenching and hybridization efficiency of BHQ to gliadin. For this process, we developed an experiment with a fluorophore (6-Carboxyfluorescein or 6-FAM) labeled gliadin specific aptamer. The gliadin from gluten was labeled with BHQ using two different reactions: BHQ-10 succinimidyl ester (NHS ester) and BHQ-10 carboxylic acid. The reaction of BHQ-10 carboxylic acid with gliadin demonstrated to be efficient for quenching the fluorophore energy by an average of 62% in comparison to the gliadin as a control. Additionally, we demonstrated the hybridization efficiency by the FRET physical phenomenon. This sets the basis for the breakthrough development of a simplified gluten detection biosensor by the elimination of the fluorophore in common biosensing systems.
11900-122
Author(s): Xuan Liu, Roberto Adamson, New Jersey Institute of Technology (United States)
On demand starting 10 October 2021
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In this study, we digitally stained volumetric optical coherence tomography (OCT) images of human breast tissues, utilizing a deep learning approach for pixel classification and coloring. Our main goal was to generate histology-like images from 3D OCT data. Our digital staining technology converts gray scale OCT images into color images and the colors at different regions resemble those in hematoxylin-eosin stained histology. This largely facilitates the interpretation of OCT data, which is critical for the clinical adoption of OCT in breast cancer diagnosis and treatment. We achieved digital staining through deep learning. We trained a U-Net to classify image pixels into diferent (dense or adipose) tissues, and trained a conditional generative adversarial network (CGAN) to create colors of dense and adipose tissue from histological images.
11900-123
Author(s): Ioanna A. Gorbunova, Maxim E. Sasin, Dmitry P. Golyshev, Marina K. Krasnopevtceva, Andrey G. Smolin, Oleg S. Vasyutinskii, Ioffe Institute (Russian Federation)
On demand starting 10 October 2021
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In this work the influence of solution polarity and viscosity on fluorescence decay parameters in free and protein-bound NADH was studied. The fluorescence of NADH in water, methanol, ethanol, propylene glycol, and alcohol dehydrogenase solutions was two-photon excited by femtosecond laser pulses at 720 nm and recorded using TCSPC system. Fluorescence decay times and the corresponding weighting coefficients were determined from fit of polarization-insensitive intensity decay. As shown fluorescence decay times τ1 and τ2 and weighting coefficients a2 and a1 was dependent on the solution. The interpretation of the results obtained was made using the quantum chemical ab initio calculations and the model describing the dependence of the nonradiative relaxation transition probabilities on the intramolecular static electric field.
11900-124
Author(s): Marina K. Krasnopevtceva, Maxim E. Sasin, Ioanna A. Gorbunova, Dmitry P. Golyshev, Andrey G. Smolin, Viktor Belik, Oleg S. Vasyutinskii, Ioffe Institute (Russian Federation)
On demand starting 10 October 2021
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Flavin adenine dinucleotide (FAD) in its oxidized (FAD+) and reduced (FADH2) forms is an important intracellular coenzyme involved in redox reactions in living cells. As known, FAD+ can fluoresce, but FADH2 cannot and therefore FAD can be used as a fluorescent probe for studying biochemical reactions in living cells. In this paper fluorescence anisotropy and decay times in FAD in water-methanol solutions have been determined from experiment under excitation at 355 and 450 nm and analysed. Theoretical models describing relaxation processes in FAD after excitation by short laser pulses were developed based on analysis of the experimental results obtained and quantum chemical calculations performed.
11900-125
Author(s): Karina Matveeva, Andrey Y. Zyubin, Elizaveta Demishkevich, Vladimir Rafalskiy, Ekaterina Moiseeva, Igor Kon, Anna Kundalevich, Victoria Butova, Ilia Samusev, Immanuel Kant Baltic Federal Univ. (Russian Federation)
On demand starting 10 October 2021
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This paper describes detailed study of spectral and time-resolved photoprocesses in human platelets and their complexes with platinum (Pt) nanoparticles (NPs). Fluorescence, quantum yield and platelet amino acid lifetime changes in presence and without femtosecond ablated platinum NPs have been studied. Fluorescence spectroscopy analysis of main fluorescent amino acids and their residues (tyrosine (Tyr), tryptophan (Trp) and phenylalanine (Phe)) belonging to the platelet membrane has been performed.
11900-126
Author(s): Andrey Y. Zyubin, Immanuel Kant Baltic Federal Univ. (Russian Federation); Anastasia Lavrova, Olga Manicheva, Marine Dogonadze, Saint-Petersburg State Research Institute of Phthisiopulmonology (Russian Federation); Vitaly Belik, Freie Univ. Berlin (Germany); Ilia Samusev, Immanuel Kant Baltic Federal Univ. (Russian Federation)
On demand starting 10 October 2021
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The Mycobacterium tuberculosis (MbT) drug resistance remains a serious problem to global public health and usually leads to a lethal outcome in patients with such strain MbT. Precise and rapid identification of a specific MbT strain is vital for the appointment of anti-tuberculosis therapy. We continue investigation [1, 2] of deactivated MbT strains of the Beijing family by Raman spectroscopy method and focus on Raman spectra simulation for potential biomarker describing strains identification. We have studied extrapulmonary Beijing strain with XDR resistance. Experimentally, samples were investigated by Raman spectrometry with He-Ne (λ=633 nm) laser excitation source in the «fingerprint» region. As a result new spectral features were determined. These differences can be described for spectral discrimination between drug-sensitive and drug-resistant strains taken from extra-pulmonary forms of tuberculosis. We suppose that the obtained results can be useful for new diagnostic tools research
Session 5: OCT and Applications
In person: 11 October 2021 • 14:30 - 15:00 China Standard Time
Session Chair: Zhihua Ding, Zhejiang Univ. (China)
11900-21
Author(s): Chaoliang Chen, Southeast Univ. (China)
In person: 11 October 2021 • 14:30 - 14:45 China Standard Time
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We present a microvascular imaging technique: Gabor optical coherence tomographic angiography (GOCTA), which calculates vascular signals from spectral domain directly, achieving low computational complexity. The data processing speed is improved of 140 times compared Speckle Variance OCT (SVOCT). We tested the performance of the proposed technique on both phantom and human retinal data, and a home-built high-speed OCT system with a swept source and graphics processing units were performed to achieve real-time microvascular imaging on human skin. All experimental results demonstrated the high-speed imaging ability of the proposed technique, and it may help improve the efficiency of disease diagnosis in clinics.
11900-23
Author(s): Jianrong Qiu, Zhihua Ding, Zhejiang Univ. (China)
In person: 11 October 2021 • 14:45 - 15:00 China Standard Time
Break
Tea/Coffee Break 15:00 - 15:30
Session 6: Biomedical Spectroscopy
In person: 11 October 2021 • 15:30 - 17:15 China Standard Time
Session Chair: Haixia Qiu, Chinese PLA General Hospital (China)
11900-24
Author(s): Yamin Lin, Mengmeng Zheng, Jiamin Gao, Xin Zhao, Fujian Normal Univ. (China); Wei Gong, Fujian Normal University (China); Yun Yu, Fujian Univ. of Traditional Chinese Medicine (China); Juqiang Lin, Fujian Normal Univ. (China)
In person: 11 October 2021 • 15:30 - 15:45 China Standard Time
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In this study, a noninvasive and sensitive prostate cancer screening method combining membrane protein purification with silver nanoparticle-based surface-enhanced Raman spectroscopy (SERS) was present. The cellulose acetate (CA) membrane was used to isolate the total serum proteins from patients with prostate cancer (n = 10) and healthy volunteers (n = 10), and then the purified serum proteins were mixed with silver nanoparticles to perform SERS detection. Furthermore, the PCA-LDA diagnostic algorithm was introduced to analyze and classify the SERS spectra acquired from cancer and normal serum proteins, with the diagnostic sensitivity and specificity of 90% and 80%, respectively. These results are very promising for developing a label-free clinical tool for prostate cancer detection and screening.
11900-25
Author(s): Rongxin Fu, Ya Su, Ruliang Wang, Xue Lin, Xiangyu Jin, Han Yang, Wenli Du, Xiaohui Shan, Wenqi Lv, Guoliang Huang, Tsinghua Univ. (China)
In person: 11 October 2021 • 15:45 - 16:00 China Standard Time
11900-26
Author(s): Yamin Lin, Jiamin Gao, Mengmeng Zheng, Dan Liu, Xin Bai, Fujian Normal Univ. (China); Yun Yu, Fujian Univ. of Traditional Chinese Medicine (China); Juqiang Lin, Fujian Normal Univ. (China)
In person: 11 October 2021 • 16:00 - 16:15 China Standard Time
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In this study, we report a novel approach for SERS analysis in body fluid detection by combining Ag NPs with HAp NPs as a substrate. SERS has a broad application prospect in the field of various cancer types detection owing to its ultrahigh detective sensitivity. Unfortunately, the SERS technology was unstable due to the maldistribution of silver nanoparticles (Ag NPs). Hence, Ag NPs was deposited on HAp NPs to prepare HAp/Ag composite SERS substrate. Then, the HAp/Ag composite SERS substrate was employed to detect some human fluid samples, including serum, urine and saliva. In summary, the HAp/Ag nanocomposite SERS substrate designed and synthesized in this paper has high SERS activity. The rapid SERS detection and analysis can be carried out by dropping the fluid samples onto the HAp/Ag substrate.
11900-27
Author(s): Jing Wang, Key Lab. of OptoElectronic Science and Technology for Medicine, Ministry of Education (China), Fujian Provincial Key Lab. of Photonics Technology (China)
In person: 11 October 2021 • 16:15 - 16:30 China Standard Time
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Liquid biopsy is a non-invasive test that analyzes the circulating biomolecules released by tumors for cancer detection and monitoring. Specifically, the overall molecular make-ups of primary and metastatic tumors could be evaluated by the comprehensive characterization of circulating biomolecules including circulating tumor cells (CTCs), extracellular vesicles (EVs), proteins, and nucleic acids, etc. However, the characterization of these circulating molecules is challenging due to their rarity and/or heterogeneity. In this contribution, I will talk about the use of surface-enhanced Raman spectroscopy (SERS) techniques for sensitive characterization of circulating molecules for improved cancer management (e.g., cancer detection, therapy monitoring, cancer risk assessment).
11900-28
Author(s): Yongqing Zhang, Yihui Zhou, Hyeon Jeong Lee, Delong Zhang, Zhejiang Univ. (China)
In person: 11 October 2021 • 16:30 - 16:45 China Standard Time
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Cholesterol has become a disturbing word for the mass population due to its connections to multiple health problems. Although the biochemical pathway is well-understood, cholesterol, as a crucial metabolite, still has many disease-related mechanisms unclear, such as subcellular regulation of cholesterol synthesis. It presents a grand challenge for conventional characterization methods, such as biochemical assay or mass spectrometry, which have difficulties providing non-invasive, high-speed, and high-resolution measurement of metabolites in living cells. In contrast, stimulated Raman scattering (SRS), enhanced by simultaneous two-photon fluorescence imaging, is capable to selectively visualize metabolic biomolecules based on vibrational spectroscopic features.
11900-29
Author(s): Qiwen Wang, Mengmeng Zheng, Yuhong Fang, Fujian Normal Univ. (China); Kecan Lin, The First Affiliated Hospital of Fujian Medical Univ. (China); Shusen Xie, Zheng Huang, Fujian Normal Univ. (China)
In person: 11 October 2021 • 16:45 - 17:00 China Standard Time
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Early diagnosis and treatment of Liver cancer play a significant role in reducing its high mortality. In this preliminary study, the feasibility of using surface enhanced Raman spectroscopy (SERS) of serum to identify primary liver cancer was explored. Serum samples were obtained from liver cancer patients and healthy controls. Nano-silver colloids were used as substrate and mixed with serum samples. Raman spectra were obtained from a confocal Raman micro-spectrometer. Analyses of spectral characteristics showed noticeable differences in peak positions between the mean spectra of the control and patients, which suggest that SERS might be useful for identifying serum markers of liver cancer.
11900-30
Author(s): Tianrun Chen, Qingyue Cheng, Hyeon Jeong Lee, Zhejiang Univ. (China)
In person: 11 October 2021 • 17:00 - 17:15 China Standard Time
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Cell classification is a fundamental task in biological research and medical practices. In this study, we proposed a single-cell classification pipeline through machine learning and hyperspectral stimulated Raman scattering imaging. The pipeline proposed is validated by using hyperspectral SRS images of two types of pancreatic cancer cells before and after the treatment of drugs that affects cellular cholesterol level. The result demonstrates that the proposed machine learning pipeline is capable of classifying cells with different metabolite dynamics, which provides possibilities for wide applications in cell analysis.
Session 7: Translational Optical Techniques for Clinical Medicine I
In person: 12 October 2021 • 08:30 - 10:00 China Standard Time
Session Chair: Dan Zhu, Wuhan National Research Ctr. for Optoelectronics (China)
11900-31
Author(s): Buhong Li, Fujian Normal Univ. (China)
In person: 12 October 2021 • 08:30 - 09:00 China Standard Time
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Singlet oxygen is widely considered to be the major cytotoxic agent, and its production and total amount during photodynamic therapy (PDT) could be served as a direct indicator for therapeutic evaluation. Therefore, the quantification of singlet oxygen generation during PDT treatment has been of great interesting over the past three decades, while the direct measurement of singlet oxygen luminescence around 1270 nm has been achieved with the high sensitive near-infrared photomultiplier tube. Most recently, considerable attention has been focused to the direct detection of singlet oxygen luminescence for PDT dosimetry. In this talk, the recent advances on the spatiotemporal detection of singlet oxygen luminescence during photosensitization will be presented, and the potential application of singlet oxygen luminescence dosimetry for clinical PDT will be discussed.
11900-32
Author(s): Lei Su, Bowen Liao, Yichun Wang, Hongzhang He, Han Wu, Xiuquan Ma, Huazhong Univ. of Science and Technology (China)
In person: 12 October 2021 • 09:00 - 09:15 China Standard Time
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Excimer laser coronary plaque ablation (ELCA) has been increasingly used due to its safety and effectiveness. The laser catheter plays an important role in the success of the operation. In this paper, the finite element simulation analysis of the mechanical properties of the laser catheter is carried out, and an innovative optimization design is carried out on this basis.
11900-33
Author(s): Peng Xiao, Zhengyu Duan, Jin Yuan, ZhongShan Ophthalmic Ctr. (China)
In person: 12 October 2021 • 09:15 - 09:30 China Standard Time
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To establish complementary information for the diagnosis and evaluation of ocular surface diseases, we developed a multi-modal non-invasive anterior eye imaging platform by combining ultra-high resolution optical coherence tomography (UHR-OCT) with a microvascular imaging system based on slit-lamp biomicroscopy. The system provided versatile structural images by performing real-time cross-sectional UHR-OCT imaging of the anterior eye, conjunctival vessel network imaging, high-resolution conjunctival blood flow videography, fluorescein staining and traditional slit-lamp imaging on a single device. With self-developed software, a conjunctival vessel network image and blood flow videography were further analyzed to acquire quantitative morphological and hemodynamics information. The ability of our multi-modal anterior eye imager to provide both structural and functional information for ophthalmic clinical applications was demonstrated on a healthy human subject and a keratitis patient.
11900-34
Author(s): Na Meng, Sen Wang, ShuLing Zou, Fujian Normal Univ. (China); Min Li, Zhejiang lnstitute of Medical Device Testing (China); Min Wang, Zheng Huang, Fujian Normal Univ. (China)
In person: 12 October 2021 • 09:30 - 09:45 China Standard Time
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In this study, four light sources were evaluated for Hemoporfin photodynamic therapy. Photobleaching experiments were carried out by exposing Hemoporfin solutions under the same power density for different lengths of time to 532 nm diode laser, 630 nm diode laser, green LED, and red LED, respectively. Hemoporfin fluorescence was measured before and after light exposure. Photobleaching kinetics showed the order of 532 nm laser > green LED > red LED > 630 nm laser. This study suggests that the power density and exposure time of different light sources need to be adjusted in order to achieve the same levels of photodynamic effect.
11900-35
Author(s): Zhiyuan Liu, Huafeng Liu, Zhejiang Univ. (China)
In person: 12 October 2021 • 09:45 - 10:00 China Standard Time
Break
Tea/Coffee Break 10:00 - 10:30
Session 8: Translational Optical Techniques for Clinical Medicine II
In person: 12 October 2021 • 10:30 - 12:00 China Standard Time
Session Chair: Changfeng Wu, Southern Univ. of Science and Technology of China (China)
11900-36
Author(s): Haixia Qiu, Chinese PLA General Hospital (China)
In person: 12 October 2021 • 10:30 - 11:00 China Standard Time
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Photodynamic therapy (PDT) is a targeting and minimally invasive treatment. It is a photochemical process combining the interactions among photosensitizer, light, and oxygen to generate cytotoxic reactive oxygen species, which is finally responsible for the PDT-mediated destruction. PDT was developed as an anticancer regiment. Nowdays, it has been widely used in many intraluminal benign and malignant diseases, such as tumor targeted-PDT for intraluminal malignant tumor and precancerous lesion. PDT can be divided into tumor targeted-PDT, Vascular-targeted PDT (V-PDT) and antimicrobial photodynamic therapy (aPDT). Tumor targeted-PDT can be used for many intraluminal malignant tumor and precancerous lesion. V-PDT can apply for some intraluminal benign vascular diseases. And aPDT can deal with some refractory microbial infection. The continuous progress of laser or optical diagnosis and treatment equipment will further improve the selectivity, safety and effectiveness of PDT.
11900-37
Author(s): Huafeng Liu, Zhejiang Univ. (China); Linlin Zhao, Zhejiang University (China)
In person: 12 October 2021 • 11:00 - 11:30 China Standard Time
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The importance of accurate attenuation correction (AC) in positron emission tomography (PET) has been widely recognized. However AC based PET/CT or PET/MR suffers from several problems such as artifacts and noises. In this paper, we propose a novel method based on physics-driven Iteration Shrinkage-Thresholding Algorithm (PISTA) to achieve quantitative PET image reconstruction from raw sinogram data without AC. The PISTA utilizes system matrix which is used as geometry of projection between quantitative PET activity images and sinograms without AC, to generate the next updated quantitative PET activity images as the input of learnable part. An evaluation study on simulated phantom data is described, where experimental results have shown great promise for such strategy.
11900-38
Author(s): Wenqi Lv, Rongxin Fu, Xue Lin, Ya Su, Xiangyu Jin, Han Yang, Xiaohui Shan, Wenli Du, Kai Jiang, Guoliang Huang, Tsinghua Univ. (China)
In person: 12 October 2021 • 11:30 - 11:45 China Standard Time
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Diabetes is one of the most common metabolic diseases in the world. The human eye plays an important role in painless and non-invasive approaches, because it is considered an internal organ but can be easily be externally observed. We developed an AI model to predict the probability of diabetes from scleral images taken by a specially developed instrument, which could conveniently and quickly collect complete scleral images in four directions and perform artificial intelligence (AI) analysis in 3 min without any reagent consumption or the need for a laboratory. With this AI eye feature imaging and analysis method, diabetic patients’ health conditions can be rapidly, non-invasively, and accurately analyzed, which offers a platform for noninvasive forecasting, early diagnosis, and long-term monitoring for diabetes and its complications. ------------------- The authors thank all funding support from National Key Research and Development Program of China (2018YFA0704000), National Natural Science Foundation of China (61927819, 81827808), Tsinghua University Spring Breeze Fund (2020Z99CFG011), the Beijing Lab Foundation, and the Tsinghua Autonomous Research Foundation (20194180031, 20201080058).
11900-39
Author(s): Yuli Li, Xianlin Song, Nanchang Univ. (China)
In person: 12 October 2021 • 11:45 - 12:00 China Standard Time
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We use the simulation software COMSOL Multiphysics to develop a computer numerical simulation model composed of water, tumor, breast tissue, subcutaneous tissue, epithelial tissue and short pulse laser sources of different wavelengths. The model studied the propagation and heat transfer of laser at 633nm, 700nm, 752nm and 900nm in the human breast. The energy transfer from the water layer to the breast tissue is described by the diffusion equation or the Helmholtz equation. The temperature change of the breast tumor and each breast tissue is obtained by solving the biological heat transfer equation. The interaction of the short pulse laser with the breast model at different wavelengths was obtained.
Break
Lunch Break 12:00 - 13:30
Session 9: Nanobiophotonics
In person: 12 October 2021 • 13:30 - 15:15 China Standard Time
Session Chair: Qiuqiang Zhan, South China Normal Univ. (China)
11900-40
Author(s): Yueqing Gu, China Pharmaceutical Univ. (China)
In person: 12 October 2021 • 13:30 - 14:00 China Standard Time
11900-41
Author(s): Changfeng Wu, Southern Univ. of Science and Technology of China (China)
In person: 12 October 2021 • 14:00 - 14:30 China Standard Time
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有机半导体材料具有优异的光电特性,广泛应用于柔性及有机光电子器件中。利用溶剂突然变化,可以将有机半导体聚合物制备出小尺寸高亮度的聚合物点荧光探针。通过调控尺寸和组分,获得了闪烁型小尺寸聚合物点,并将该类探针用于光学涨落超分辨成像中。利用染料掺杂的方法对各种聚合物纳米粒子进行了光学性质的调控,通过聚合物和染料分子之间的能量传递可以获得高灵敏的生物传感器,用于体内小分子的高灵敏实时检测。设计窄带隙有机半导体,提高近红外二区发光量子效率,实现了可穿透颅骨的高清晰小鼠脑血管成像。
11900-42
Author(s): Cuiping Yao, Xi'an Jiaotong Univ. (China)
In person: 12 October 2021 • 14:30 - 15:00 China Standard Time
11900-43
Author(s): Weikai Zhang, Zewen Wei, Qin Li, Beijing Institute of Technology (China)
In person: 12 October 2021 • 15:00 - 15:15 China Standard Time
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Acquisition of the genes encoding variable regions of paired heavy and light chains (VH:VL) is crucial, but it is a labor and cost-intensive process in traditional methods. This study presents a novel method in which all processing steps for acquiring natively paired VH:VL genes from single cells are finished in a single microfluidic chip. The microfluidic chip performs single-cell trap/in situ fluorescent examination of antibody specificity/cell lysis/gene amplification all at single-cell level. By a proof-of-concept validation of efficiently acquiring paired VH:VL genes of anti-RBD (which is a key protein of SARS-CoV-2 virus) mAbs from single hybridomas, the microfluidic chip has been proved capable of remarkably improving cell loss/human labor/time cost, and more importantly, determinacy of native VH:VL genes pairing which is one of the most decisive factors of effectiveness for antibody discovery.
Break
Tea/Coffee Break 15:15 - 15:45
Session 10: Photoacoustic Imaging
In person: 12 October 2021 • 15:45 - 16:45 China Standard Time
Session Chair: Puxiang Lai, The Hong Kong Polytechnic Univ. (Hong Kong, China)
11900-44
Author(s): Liming Nie, Guangdong General Hospital (China)
In person: 12 October 2021 • 15:45 - 16:15 China Standard Time
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Photodynamic therapy and adipose browning induction are two promising approaches to reverse obesity. The former strategy acts rapidly and locally, whereas the latter has a more gradual and widespread effect. Despite their complementarity, they have rarely been combined and imaged non-invasively in vivo. Here we introduce an adipose-targeting hepatitis B core protein complex that contains a traceable photosensitizer and a browning agent that allows simultaneous photodynamic and browning treatments, with photoacoustic molecular imaging. After intravenous injection in obese mice drives adipose reduction thanks to the synergy of ZnPcS4 photodynamics and rosiglitazone browning. Using photoacoustic molecular imaging, we could monitor the changes induced by the treatment, which included complex activity, lipid catabolism and angiogenesis. Our findings demonstrate the anti-obesity potential of our feedback-based synergic regimen orchestrated by the targeted hepatitis B core complex.
11900-47
Author(s): Yubin Liu, Fujian Normal Univ. (China)
In person: 12 October 2021 • 16:15 - 16:30 China Standard Time
11900-48
Author(s): Jianpan Gao, Handi Deng, Hanqing Duan, Haoming Huo, Tsinghua Univ. (China); Yizhou Bai, Bin Luo, Beijing Tsinghua Changgung Hospital (China); Cheng Ma, Tsinghua Univ. (China)
In person: 12 October 2021 • 16:30 - 16:45 China Standard Time
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Articulated arms provide an alternative light delivery option which potentially offer high transmission efficiency, stable and flexible operation, and low cost. Despite its wide applications in cosmetology, articulated arms for light delivery were understudied in the PAI community. In this paper, we reported the fabrication and experimental evaluation of an articulated arm specifically designed for linear-array-based PAI. Without losing the flexibility provided by the linear probe, the new illumination strategy has increased transmission efficiency and improved beam quality. Moreover, the articulated arm can be equipped with a number of spatial positioning devices to perform three-dimensional reconstructions in the future.
11900-45
Author(s): Samuel John, Nasrin Nesha, Yoseph Adie, Wayne State Univ. (United States); Loay S. Kabbani, Henry Ford Health System (United States); Mohammad Mehrmohammadi, Wayne State Univ. (United States)
On demand starting 10 October 2021
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It is estimated that more than 400,000 gastrostomy tubes (G-tubes) are placed annually in the United States. Currently, image-guidance technologies such as endoscopy, fluoroscopy, and ultrasound (US) imaging are used for placing G-tubes. However, the poor visualization of vital organs such as the colon, ionizing radiation in fluoroscopy, and US imaging artifacts causes complications and limits the efficacy of image-guided G-tube placement procedures. Moreover, the majority of these procedures are not point-of-care and require equipped facilities. Herein, we propose to develop a safe, point-of-care solution based on Photoacoustic (PA) imaging which utilizes a combination of tissue’s endogenous contrast and an exogenous contrast agent for the accurate guidance of the G-tube placement. Our preliminary studies indicated that PA imaging accurately detected the dye-coated colon-mimic, providing high contrast, artifact-free images of the introducer needle superimposed on the tissue background
11900-46
Author(s): Yan Yan, Jose Galaz, Maryam Basij, Nardhy Gomez-Lopez, Mohammad Mehrmohammadi, Wayne State Univ. (United States)
On demand starting 10 October 2021
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This work presents a non-invasive imaging method, combined ultrasound and photoacoustic (PA), to evaluate cervical remodeling at the molecular level by assessing relative collagen and water content variations. A longitudinal study was performed in murine cervices (n=3/group) acquired at different time points from non-pregnant to postpartum. Excised cervical tissues were scanned spectroscopically across the wavelengths covering the peak absorption of collagen and water (1100 to 1650 nm). A custom-designed spectral unmixing method was utilized to calculate the collagen-to-water ratio (CWR). Histology analysis by H&E and Sirius Red stains were used to validate the collagen content of the spectroscopic PA results, which reveal that the non-pregnant murine cervices have significantly higher CWR than any other groups. The CWR is decreasing along with the process of cervical ripening. The process of cervical tissue repairing shows an increment in CWR until ~4 days after postpartum.
Conference Chair
Hainan Univ. (China)
Conference Chair
Johns Hopkins Univ. (United States)
Conference Chair
Ying Gu
Chinese PLA General Hospital (China)
Conference Co-Chair
Wuhan National Research Ctr. for Optoelectronics (China)
Program Committee
Beijing Institute of Technology (China)
Program Committee
The Chinese Univ. of Hong Kong (Hong Kong, China)
Program Committee
The Univ. of Oklahoma (United States)
Program Committee
Univ. of Maryland, College Park (United States)
Program Committee
Zhihua Ding
Zhejiang Univ. (China)
Program Committee
Fujian Normal Univ. (China)
Program Committee
Huafeng Liu
Zhejiang Univ. (China)
Program Committee
Tsinghua-Berkeley Shenzhen Institute (China)
Program Committee
Univ. at Buffalo (United States)
Program Committee
Zhejiang Univ. (China)
Program Committee
Shenzhen Univ. (China)
Program Committee
Peking Univ. (China)
Program Committee
Zhejiang Univ. (China)
Program Committee
Saratov State Univ. (Russian Federation)
Program Committee
Univ. of Washington (United States)
Program Committee
Univ. of Michigan (United States)
Program Committee
Peking Univ. Health Science Ctr. (China)
Program Committee
South China Normal Univ. (China)
Program Committee
Tianjin Univ. (China)
Program Committee
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences (China)
Program Committee
Xi'an Jiaotong Univ. (China)
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