Applied Image, Inc.


Precision imaged optical components and calibration standards. For the design, evaluation, and calibration of photonics-based systems. Single-part prototypes to thousands of OEM parts. Production and certification of NIST Traceable barcode verifier calibration standards. Image Quality Testing Standards. Custom optical components and standards, Test Targets and Charts, Photomasks, or Barcodes. ISO 9001:2015, ISO/IEC 17025:2017 accredited for measurement, grading, and calibration of barcodes.
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Applied Image, Inc.
1653 E Main St Ste 1
Rochester, NY
United States


04 February 2021
Angular Measurement Accreditation Announcement
APPLIED IMAGE, a leading manufacturer of NIST Traceable Calibration Standards, announced that its Rochester-based laboratory added angular measurement to its ISO/IEC 17025:2017 accreditation. ISO/IEC 17025 is the highest recognized quality standard in the world for calibration and testing laboratories. For an organization to be accredited in ISO/IEC 17025, an accreditation board must determine their lab can consistently produce precise and accurate measurements and implement a rigorous quality management system. This standard also requires employees to be assessed as skilled to perform all testing on the scope of accreditation. Having passed the ANSI National Accreditation Board’s stringent surveillance audit, APPLIED IMAGE has expanded its accreditation and can now offer its customers angular measurements from its own accredited lab. “We’ve always offered angular measurements, but now we can keep the service in-house at our accredited lab. This means we keep costs down and cut at least a week out of the project timeline; we can turn that project step around in a day,” said Gary Klimek, quality manager at APPLIED IMAGE. Klimek knows a lot goes into calculating the uncertainties of angular measurement. Compared to other measurements, it’s a bit more challenging. As part of the audit, labs must create repeatability studies that the auditors then watch. Klimek was proud of his team of quality control technicians. He noted that the auditor was pleased with the technicians’ testing performance and the process and the quality system they had in place. Klimek said, “It meant a lot to hear the auditor say ‘You have a good level of quality and you know what you’re doing.’“ Darren Dixon, the technical sales manager at APPLIED IMAGE, was excited about this addition to the scope, too; he said, “a lot of customers request this service, so having the accreditation satisfies their requirements.” The technical sales team is ready to put this new accreditation to use for customers. Adding angular measurement to its 17025 accreditation confirms the demonstrated and ongoing commitment to reliability and quality assurance at APPLIED IMAGE. For more details, contact APPLIED IMAGE online, by email, or phone (585) 482-0300.
04 February 2021
APPLIED IMAGE Launches a New NIST Traceable Color Calibration Slide for Whole Slide Imagers
APPLIED IMAGE, pre-eminent manufacturer of NIST Traceable Calibration Standards, launched a newly designed color calibration slide that will help standardize whole slide imaging analysis. The new NIST Traceable, Whole Slide Imaging Color Transmission Calibration Standard (p/n IAM-9C-WSI) is designed to help researchers calibrate, standardize, and trace color settings for imaging analysis. With this addition, APPLIED IMAGE now offers two choices for color calibration standards for digital pathology. Market feedback indicated that the whole slide imager market would benefit from modifications to the original IAM-9C slide. This new design consists of a modified and simpler color palette, clear background, and thinner design which would help users scan the slide with their WSI in automated mode. This benefit is especially useful for histology, pathology, food processing, and other applications. “Without this type of technology, researchers have to rely on visual inspection to match their system to the correct color profile, that creates a wide range of variability,” said Glenn Jackling CEO, APPLIED IMAGE. Perception of color is one reason why it’s not uncommon for two labs to return different results. Developing standards for color calibration and color measurement related to digital pathologies has been an elusive objective for many years for the industry. APPLIED IMAGE has leveraged its 40 years of calibration standard development to help create a NIST traceable approach to color calibration by measuring and reporting spectral transmission data for each color patch in 5nm increments, from 340nm to 830nm. The International Workshop on Harmonization and Standardization of Digital Pathology (April 2019) reported that “Standardization of color in Digital Pathology Image and scanners is being demanded by various experts” in the field. At the workshop, Prof. Masahiro Yamaguchi (Tokyo Institute of Technology) addressed the importance and challenges of standardization of color pathology image analysis. Staining and scanning processes cause color variation; industry leaders and experts must address color variation issues in digital pathology. APPLIED IMAGE is dedicated to using its expertise in calibration technology to bring reliable tools to the whole slide imaging market to reduce variability and help researchers set baseline reference standards and color measurements that are traceable. The NIST Traceable, Whole Slide Imaging Color Transmission Calibration Standard (p/n IAM-9C-WSI) supports that effort. The new IAM-9C-WSI slide is unique in the marketplace, providing calibrated and traceable spectral transmission data for each color on the slide. IAM-9C-WSI Modification The NIST Traceable Color Transmission Calibration Slide (p/n IAM-9C), launched in 2018, now has a thinner design. This thinner design will help users achieve better fit, focus, and functionality when the slide is used with industry-standard equipment. “By offering both slide designs, we’ve made it easier for customers to achieve first-time use success. We’re excited that we have products that customers can use in their current image analysis or whole-slide-imaging applications. Having a NIST traceable color calibration standard is the critical first step in ensuring color standardization in both intra- and inter-lab environments,” said Glenn Jackling CEO, APPLIED IMAGE. IAM-9C-WSI and IAM-9C- Description Microscopy color patches for assessing color accuracy. NIST traceable calibration data for each large color patch is supplied as spectral transmission. By using the supplied spreadsheet, this data can be converted to the color space you need (such as Adobe RGB 1998, L*A*B*, XYZ, etc.) The film is permanently sandwiched between glass; atmospheric deterioration of the measured values is minimized. Each slide is individually serialized and calibrated. Both slides are mounted on a base of D263 optical glass, with an overall thickness of under 1.2MM. The products include Calibration Certificate, Calibration Data Report, Conversion Spreadsheet, USB Flash Drive with Data and Spreadsheet, and Protective Case. The APPLIED IMAGE facility is ISO 9001:2015 certified, and its calibration lab is ISO 17025:2017 accredited, ensuring high quality, accurate, and consistent performance. The NIST Traceable Color Transmission Calibration Slide, part number IAM-9C-WSI is available for direct order, here and part number IAM-9C, is available for direct order, here. Our sales team can be reached by email, or phone (585) 482-0300. About APPLIED IMAGE Inc. APPLIED IMAGE is a global manufacturer of precision-imaged optical components and calibration standards for a wide range of industries and applications. Whether you require NIST Traceable calibration standards, color targets, precision-imaged optical components to test, calibrate, align, control, or measure your optics, photonics, or imaging systems, or need OEM components to work within your product design, we can support your unique requirements.
04 February 2021
APPLIED IMAGE Has Updated the T-45-1-RM Product Line to the New 2020 Standard.
APPLIED IMAGE Has Updated the T-45-1-RM Product Line to the New 2020 Standard. Rochester, NY June 9, 2020 APPLIED IMAGE, a global manufacturer of precision imaged optical components and calibration standards, is pleased to announce the official rollout of the T-45 ANSI/ISEA Z87.1-2020 Test Resolution Standard (p/n T-45-1-RM). The company has updated the T-45-1-RM target to comply with the new 2020 industry standard. The critical update to the product is the addition of the “17” Ronchi patterns on the target. APPLIED IMAGE is one of the few, if not only, manufacturers of this ISO-compliant test target. The internal team made a special effort to make this product available within weeks of the standard update. “We wanted to be the first to make this product available, so our customers could follow and meet the requirements of the 2020 standard. Our mission is to ensure customers have the tools they need to offer products compliant with the latest standards consistently,” said Brandon Morabito, sales engineer, APPLIED IMAGE. Orders for the T-45-1-RM product have already come through. The target consists of 24 Sunburst Lines, 0.25mm x 30mm, with a center crosshair target area and three off-center vertical and horizontal Ronchi gratings. These features consist of 0.68cy/mm, 0.8cy/mm and 1.6cy/mm bars (which are then 17cy/mm, 20cy/mm and 40cy/mm after 25 X reduction). For a detailed product description or to order directly, visit Contact our sales team at or (585) 482-0300 ext. 219 for additional questions or support.