Development of a microfluidic system for measuring HIV-1 viral load
The World Health Organization (WHO) is rapidly expanding antiretroviral treatment (ART) in sub-Saharan countries.
However, virological failure of ART is rarely monitored due to the lack of affordable and sustainable viral load assays
suitable for resource-limited settings. Here, we report a prototype of a rapid virus detection method based on
microfluidic technologies. In this method, HIV-1 particles from 10 μL whole blood were captured by anti-gp120
antibody coated on the microchannel surface and detected by dual fluorescence signals under microscopy. Next,
captured HIV-1 particles were counted using the free software, ImageJ (http://rsbweb.nih.gov/ij/). This rapid HIV-1
detection method has potential to be further developed for viral load monitoring at resource-limited settings.
This paper was published in SPIE Proceedings Vol. 7666
Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Security and Homeland Defense IX, Edward M. Carapezza, Editors, 76661H