Optical coherence tomography (OCT) holds great promise as a routine research tool for 3-D analysis of mammalian embryos. However, despite the depth penetration afforded by this imaging modality, light attenuation in tissues imposes limitations. Here we studied the optical clearing effect of different concentrations of glycerol in mouse embryos. Depth- and time-resolved profiles for OCT signal enhancement are presented. We found that application of 50% glycerol resulted in 51.5±12.5% improvement of the OCT s
DOI: 10.1117/12.808055Current SPIE Digital Library subscribers
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