Microfluidics, BioMEMS, and Medical Microsystems VII, Wanjun Wang, Editors, 72070F
Date: 23 February 2009
Paper Abstract
This paper examines the necessary technologies to be mastered in order to build a practical micro array-based
immunoassay cassette and its processing station for protein analysis. The interdependence of surface-chemistry, dye
stability and imaging are outlined showning why a treated 100 nm film of Nitrocellulose adhered by an intervening layer
to glass offers an efficacious surface for immobilizing an array of protein probes. The properties of a storage surface to
support in desiccated form, fluidize and transport additional reagents are outlined and a practical solution proposed. Wet
and Dry imaging are compared. The steps and functions expected for an assay platform comprising processing station
and biochip cassette are identified. The performance of a successful bench-top automated multiplex immunoassay
system is briefly described
DOI: 10.1117/12.806639 Current SPIE Digital Library subscribers click here to download this paper.
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