Technical considerations in quantitative blood oxygenation measurement using photoacoustic microscopy in vivo (Proceedings Paper)

Using peak amplitude spectral PA measurements in the range of 570 - 600 nm, we found it possible to quantify blood oxygenation levels. Visible light illumination minimizes the inversion error of the PA measurements. Owing to high blood absorption in this optical regime, there is also an improved signal-to-noise ratio and less influence from optical scattering. To arrive at correct, and vessel size independent, SO2 measurements, the central frequency of the ultrasonic transducer must be high enough to satisf

DOI: 10.1117/12.646265
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