Light-scattering microscope as a tool to investigate scattering heterogeneity in tissue
Rayleigh light scattering has not yet been used for quantitative investigations of heterogeneous systems. Preconditions such an experiment are a well defined scattering geometry and independent information about the local state of the sample. We have designed a new instrument that meets these criteria: a light-scattering microscope with simultaneous imaging. We demonstrate the ability to characterize local differences within one tissue type as well as global differences between tissue types. Real space images of the sample are taken by normal video microscopy techniques. The light scattering pattern in analyzed by the evaluation of wave-vector dependence and scattering direction of the scattered intensity. Statistical analysis of scattering patterns show what is important for the characterization and classification of tissues and heterogeneous structures. Real space images provide context for scattering analysis. The light scattering microscope is a powerful tool for characterization of local structural order in inhomogeneous structures like tissues.
This paper was published in SPIE Proceedings Vol. 3917