The Moscone Center
San Francisco, California, United States
28 January - 2 February 2017
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Translational Research Presentations

Nano/Biophotonics BiOS Program Track, LASE, and OPTO
(ordered by start date and time)

Ultrasound-guided photoacoustic imaging of lymph nodes with biocompatible gold nanoparticles as a novel contrast agent
Paper 10078-16

Author(s):  In-Cheol Sun, Georgia Institute of Technology (United States), et al.
Conference 10078: Colloidal Nanoparticles for Biomedical Applications XII
Session 4: Applications of Nanoparticles in Cancer Theranostics I
Date and Time: Saturday, January 28, 2017, 4:30 PM

We develop a lymph node mapping technique using ultrasound-guided photoacoustic imaging assisted with glycol-chitosan-coated gold nanoparticles as a contrast agent. In vitro dark-field microscopy studies demonstrated enhanced cellular uptake of nanoparticles by macrophages. Strong photoacoustic signal from labeled cells was observed in tissue-mimicking cell phantoms. The in vivo ultrasound-guided photoacoustic imaging of cervical lymph nodes indicated a strong PA signal from a lymph node 10 min post-injection while the signal intensity and the nanoparticle-labeled volume of tissue within the lymph node continued to increase until 4h post-injection. Histological analysis further confirmed the accumulation of gold nanoparticles within the lymph node.

Photothermal technique to damage cancer cells: in vitro studies of simultaneous pulsed and cw irradiation
Paper 10078-17

Author(s):  Noe Zamora-Romero, Univ. of California, Riverside (United States), et al.
Conference 10078: Colloidal Nanoparticles for Biomedical Applications XII
Session 4: Applications of Nanoparticles in Cancer Theranostics I
Date and Time: Saturday, January 28, 2017, 4:50 PM

This work studies the effects of simultaneous pulsed laser and continuous wave (cw) laser irradiation of prostate cancer cells incubated with gold nanorods (5.7 X10 10 p/ml), evaluated through viability testing. We hypothesize that the simultaneous use of pulsed and cw lasers may have synergistic effects since there are two techniques involved to damage the cells: hyperthermia by the cw laser and cavitation by the pulsed laser. The hyperthermia effect of the cw laser not only damages some cells by causing apoptosis due to the heat shock, but more importantly, it could increase their sensitivity to the mechanical stress caused by the shock wave generated from inducing cavitation generated in the solution by the pulsed laser irradiation.

Photonic crystal fiber-generated coherent supercontinuum for fast stain-free histopathology and intraoperative multiphoton imaging
Paper 10094-1

Author(s):  Haohua Tu, Univ. of Illinois at Urbana-Champaign (United States), et al.
Conference 10094: Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XVII
Session 1: Novel Biomedical Applications
Date and Time: Sunday, January 29, 2017, 8:30 AM

Standard histopathology and related intraoperative assessments, both widely used in cancer diagnosis and delineation of cancerous tissue, have long been plagued by slow speed and inadequate molecular information. Using coherent supercontinuum generation by photonic crystal fibers, we developed fast (minute-scale) stain-free histopathology with diverse molecular information useful for disease/cancer diagnosis, enabling intraoperative multiphoton imaging that may improve cancer surgeries. Novel imaging-based cancer biomarkers have emerged from the use of this technology, and due to laser-microscope alignment decupling, selective imaging of different biomolecules can be performed by programming without chemical labeling or optical realignment.

Precision resection of lung cancer in a sheep model using ultrashort laser pulses
Paper 10094-5

Author(s):  Rainer J. Beck, Heriot-Watt Univ. (United Kingdom), et al.
Conference 10094: Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XVII
Session 1: Novel Biomedical Applications
Date and Time: Sunday, January 29, 2017, 9:30 AM

We present the resection of lung cancer by means of picosecond laser pulses as a potential alternative to overcome limitations of current surgical techniques in terms of precision and thermal damage. Robust process parameters for the laser resection are demonstrated using tumour tissue from ovine pulmonary adenocarcinoma, a large animal model for human lung adenocarcinoma. Cancerous tissue is resected with only minimal collateral damage to the surrounding tissue and a precisely controlled depth. Additionally, deliberate heat transfer to the tissue can be achieved by modifying the laser parameters for coagulation.

Dual activity of carbon nanotube/CpG: inhibition of glioma migration and stimulation of macrophages
Paper 10078-22

Author(s):  Jacob M. Berlin, City of Hope Beckman Research Institute (United States), et al.
Conference 10078: Colloidal Nanoparticles for Biomedical Applications XII
Session 6: NP Applications in Ophtalmology and Neuroscience
Date and Time: Sunday, January 29, 2017, 11:10 AM

Even when treated with aggressive current therapies, most patients with glioblastoma survive less than two years. We previously reported that single-walled carbon nanotubes (SWCNTs) can be used to dramatically increase the immunotherapeutic efficacy of CpG oligonucleotides in a mouse model of glioma. Recently we showed that the activity observed was due to a complex mixture. Here we show that a simplified SWCNT/CpG construct not only maintains its immunostimulatory property and enhances the migration of macrophages, but surprisingly also inhibits the migration of glioma cells.

Preparation and physical characterization of magnetite nanoparticles (MNP) with aminosilane shell
Paper 10078-50

Author(s):  Maryam Youhannayee, Heinrich-Heine-Univ. Düsseldorf (Germany), et al.
Conference 10078: Colloidal Nanoparticles for Biomedical Applications XII
Session PSun: Posters-Sunday
Date and Time: Sunday, January 29, 2017, 5:30 PM

Magnetite Nanoparticles are widely studied because of their fascinating application and also biocompatibility and low toxicity in different fields of biology and medicine. These nanoparticles covered with Aminosilane are perfect candidates for hyperthermia therapy due to a nearly perfect prevention of wash-out. In this contribution, the synthesis of magnetite nanoparticles by chemical coprecipitation is presented. In a first step, magnetite nanoparticles were prepared by coprecipitation of 𝐹𝑒2+ and 𝐹𝑒3+ with ammonium. Subsequently, magnetite nanoparticles were coated with a ligand shell consisting of Aminosilane. Different techniques were used for morphology and structure characterization. Dynamic light scattering technique was carried out to investigate the size distribution of nanoparticles in wet environment. TEM images also prove the formation of spherical MNP. For characterizing the crystal structure, X-ray diffraction was used. These measurements show that different parameters such as preparation speed have a significant influence on the size of nanoparticles.

Spatially selective depleting tumor-associated negative regulatory T-cells with near infrared photoimmunotherapy (NIR-PIT): A new cancer immunotherapy
Paper 10079-1

Author(s):  Hisataka Kobayashi, National Cancer Institute (United States), et al.
Conference 10079: Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX
Session 1: NIR in Imaging and Theranostics
Date and Time: Monday, January 30, 2017, 8:00 AM

Near infrared photoimmunotherapy (NIR-PIT) is a new type of molecularly-targeted photo-therapy based on monoclonal antibody (mAb)-photoabsorber conjugates. When exposed to NIR light, the conjugate rapidly induces a highly-selective cell death only in receptor-positive, mAb-conjugate-bound cells. CD4+CD25+Foxp3+ regulatory T-cells (Tregs) are well-known immune-suppressor cells that play a key role in tumor immuno-evasion and have been the target of systemic immunotherapies. We used CD25-targeted NIR-PIT to selectively deplete Tregs, thus activating CD8+-T and NK cells and restoring local anti-tumor immunity. This not only resulted in regression of the treated tumor but also induced responses in separate untreated tumors.

New approaches for synthesis and processing of two-dimensional materials
Paper 10093-2

Author(s):  Masoud Mahjouri-Samani, Oak Ridge National Lab. (United States), et al.
Conference 10093: Synthesis and Photonics of Nanoscale Materials XIV
Session 1: Synthesis and Optoelectronic Properties of 2D Materials and Heterostructures I
Date and Time: Monday, January 30, 2017, 9:00 AM

We demonstrate the use of non-equilibrium laser-based approaches as a versatile method for the controlled synthesis and processing of 2D layered semiconductors. Tunable kinetic energy of the laser-generated precursors is used for the formation of either atomic species in vacuum or stoichiometric nanoparticles in background gases. Utilizing the stoichiometric nanoparticles as feedstocks, we demonstrate the formation of either small domain nanosheet networks (~ 20 nm) or large crystalline domains (~100 µm). Atomic species with high kinetic energies, on the other hand, are used in doping, alloying and formation of lateral heterojunction within monolayer 2D crystals. Moreover, we show a bottom-up synthesis of single crystalline monolayers of two-dimensional MoSe2-x with controllable levels of Se vacancies far beyond intrinsic levels (up to 20 %) with unique optical and electrical properties.These non-equilibrium laser-based approaches provide unique synthesis and processing opportunities that are not easily accessible through conventional methods.

Silica passivated conjugated polymer nanoparticles for biological imaging applications
Paper 10079-9

Author(s):  Struan Bourke, King's College London (United Kingdom), et al.
Conference 10079: Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX
Session 2: Nano in Imaging
Date and Time: Monday, January 30, 2017, 11:30 AM

Colorectal and prostate cancers are major causes of cancer-related death, with early detection key to increased survival. However as symptoms occur during advanced stages and current diagnostic methods have limitations, there is a need for new probes that remain bright, are biocompatible and can be targeted. Conjugated polymer nanoparticles have shown great promise in biological imaging due to their unique optical properties. We have synthesised small, very bright, very photostable CN-PPV, nanoparticles encapsulated with poloxamer polymer and a thin silica shell that have shown good cellular uptake with limited cytotoxocity. These can be further functionalised for target specific binding.

Towards biological ion imaging in vivo: potassium selective photoacoustic nanosensor
Paper 10077-4

Author(s):  Chang H. Lee, Univ. of Michigan (United States), et al.
Conference 10077: Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIV
Session 1: Biosensing Nano-structures/Particles I
Date and Time: Monday, January 30, 2017, 3:00 PM

Optically based nanosensors, such as Photonic Explorers for Bioanalysis with Biologically Localized Embedding (PEBBLEs), have been optimized for fluorescence microscopy imaging and applied for imaging numerous biochemical analytes. In here, we report the first example of a potassium selective nanosensor optimized for photoacoustic (PA) imaging. Notably, this first example of PA potassium sensor can be easily applied to other biologically relevant ions, cations and anions (e.g., sodium, magnesium, calcium, chloride and nitrite), based on the standard principles of ion selective optodes, and thus to their utilization for in vivo biological ion imaging.

Rhodamine-based activatable protease probes for fluorescence and photoacoustic cancer imaging
Paper 10079-15

Author(s):  Ryu J. Iwatate, The Univ. of Tokyo (Japan), et al.
Conference 10079: Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX
Session 5: Fluorescence Applications
Date and Time: Monday, January 30, 2017, 3:10 PM

To achieve rapid and sensitive detection of cancer during surgery, we have developed activatable fluorescent probes targeting proteases that are overexpressed in various types of cancer based on the hydroxymethyl rhodamine green (HMRG) scaffold. Here, we introduce a strategy to rationally develop activatable protease probes with desired properties based on novel asymmetrically modified rhodamines, to broaden the scope of these probes from detection to diagnosis and photoacoustic imaging.

Plasmonic distributed feedback cavity with a phase shift on single-mode optical fiber end facet for label-free biosensing
Paper 10080-15

Author(s):  Zeyu Lei, Shanghai Jiao Tong Univ. (China), et al.
Conference 10080: Plasmonics in Biology and Medicine XIV
Session 3: Plasmonics and Biomedical Applications
Date and Time: Monday, January 30, 2017, 4:20 PM

Plasmonic distributed feedback (DFB) cavities with a phase shift section have been designed and fabricated on the end facets of single-mode fibers, providing a defect state in the surface plasmon polariton band gap. Such fiber end facet SPR devices can have almost comparable biosensing capabilities to the prism coupled SPR devices, in spite of strong diffraction of the fiber guide mode. Detection of refractive index change and real-time monitoring of biomolecule binding are reported.

Light assisted drying (LAD) for protein stabilization: optimization of laser processing parameters
Paper 10081-26

Author(s):  Madison A. Young, The Univ. of North Carolina at Charlotte (United States), et al.
Conference 10081: Frontiers in Biological Detection: From Nanosensors to Systems
Session 6: New Reagents, Techniques, and Diagnostics
Date and Time: Monday, January 30, 2017, 4:40 PM

The goal of this project is to develop a novel light-based processing method to dehydrate biologics (vaccines, proteins, etc.) in preparation for long-term storage at near ambient temperatures. Refrigeration and freezing are currently the standard for the preservation of a wide variety of biologics, but these methods can be challenging in many environments due to a lack of available resources. Light-assisted drying offers a relatively inexpensive, compact method for initial processing and dry-state preservation allows for low-maintenance storage after samples have been packaged.

Modeling of transdermal fluorescence measurements from first-in-human clinical trials for renal function determination using fluorescent tracer agent MB-102
Paper 10079-17

Author(s):  Kimberly M. Shultz, Triple Ring Technologies, Inc. (United States), et al.
Conference 10079: Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IX
Session 6: Fluorescence and Label Free Detection: Clinical Applications
Date and Time: Monday, January 30, 2017, 5:00 PM

A pharmacokinetic and optical model was constructed to simulate the transdermal measurement of the fluorescent tracer agent MB-102. This agent is used to noninvasively measure kidney function. Good agreement with data from both in vitro benchtop and in vivo human clinical studies is seen. The model was developed to evaluate tracer agent equilibration kinetics and optimize design of the optical geometry. The model comprises pharmacokinetics of agent distribution within body compartments, local diffusion of the agent within the skin, Monte Carlo photon transport through tissue, and ray tracing of the instrument optics.

Remote optical stethoscope and optomyography sensing device
Paper 10077-50

Author(s):  Zeev Zalevsky, Bar-Ilan Univ. (Israel), et al.
Conference 10077: Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIV
Session PMon: Posters-Monday
Date and Time: Monday, January 30, 2017, 5:30 PM

In this paper we present the usage of photonic remote device for sensing nano vibrations for detection of muscle contraction and fatigue, eye movements and in-vivo estimation of glucose concentration. The same concept is also used to realize a remote optical stethoscope. The advantage of doing the measurements from distance is in preventing passage of infections as in the case of optical stethoscope or in the capability to monitor e.g. sleep quality without disturbing the patient. The remote monitoring of glucose concentration in the blood stream and the capability to perform optomyography for the Messer muscles (chewing) is very useful for nutrition and weight control. The optical configuration for sensing the nano vibrations is based upon analyzing the statistics of the secondary speckle patterns reflected from various tissues along the body of the subjects. Experimental results present the preliminary capability of the proposed configuration for the above mentioned applications.

Surface modified gold nanoparticles for SERS based detection of vulnerable plaque formations
Paper 10077-7

Author(s):  Christian Matthäus, Leibniz-Institut für Photonische Technologien e.V. (Germany), et al.
Conference 10077: Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIV
Session 2: Biosensing Nano-structures/Particles II
Date and Time: Tuesday, January 31, 2017, 8:20 AM

Atherosclerosis is closely related to the majority of cardiovascular diseases. One of the greatest challenges in the risk assessment of atherosclerotic depositions is the detection and recognition of vulnerable plaques. It has been hypothesized that the amount of macrophages relates to the overall plaque stability. As phagocytes, macrophages act as recipients for nanoscale particles or structures. A very sensitive strategy for probing gold nanoparticles is by utilizing surface enhanced Raman scattering (SERS). SERS labeled gold nanoparticles have been synthesized and the uptake dynamics and efficiency on macrophages in cell cultures has been studied using Raman microscopic imaging.

Blood pulse wave velocity and pressure sensing via fiber based and free space based optical sensors
Paper 10077-8

Author(s):  Zeev Zalevsky, Bar-Ilan Univ. (Israel), et al.
Conference 10077: Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIV
Session 2: Biosensing Nano-structures/Particles II
Date and Time: Tuesday, January 31, 2017, 8:50 AM

In this paper, we introduce two techniques for non-contact sensing of vital bio signs. In the first approach the optical sensor is based on single mode in-fibers Mach-Zehnder interferometer (MZI) to detect heartbeat, respiration and pulse wave velocity (PWV). The introduced interferometer is based on a new implanted scheme. It replaces the conventional MZI realized by inserting of discontinuities in the fiber to break the total internal reflection and scatter/collect light. The proposed fiber sensor was successfully incorporated into shirt to produce smart clothing. The measurements obtained from the smart clothing could be obtained in comfortable manner and there is no need to have an initial calibration or a direct contact between the sensor and the skin of the tested individual. In the second concept we show a remote noncontact blood pulse wave velocity and pressure measurement based on tracking the temporal changes of reflected secondary speckle patterns produced in human skin when illuminated by a laser beams.

Intraoperative NIR tomography system based on spatially modulated illumination using the DLP4500 evaluation module
Paper 10117-13

Author(s):  Sang Hoon Chong, Univ. of Pennsylvania (United States), et al.
Conference 10117: Emerging Digital Micromirror Device Based Systems and Applications IX
Session 4: Biomedical Imaging using a DMD or other Light Structuring Devices: Joint Session with Conferences 10068 and 10117
Date and Time: Tuesday, January 31, 2017, 11:10 AM

We constructed a novel imaging system using a digital micro-mirror device (DLP4500) for quasi real-time fluorescence image guided surgery. It enables 3D fluorescence tumor imaging based on spatial frequency domain techniques. We discuss data acquisition corrections for spatial inhomogeneity and gamma adjust, and present images from tissue phantom and in-vivo experiments.

Do piezoelectric and piezomagnetic sensors help in simulation of stress propagation prior to seismic events and hence display preseismic signature?
Paper 10105-28

Author(s):  Umesh P. Verma, Patna Science College (India), et al.
Conference 10105: Oxide-based Materials and Devices VIII
Session 10: Piezoelectric Properties and Applications
Date and Time: Tuesday, January 31, 2017, 2:45 PM

Pizeolecrtric and Pizeomagnetic materials like pbtio2,quartz, PbGatio2gallium titanate are quite susceptible to the im;lied stress and respond proportionately in direction and magnitude to the electric voltage.The intensity ,na magnitude and direction depends on the configuration and dimension of the mnaterial configurated,For desirted voltage a demarcated coupling factor are designed to respond accordingly.similar way the pizeomagnetic substances are reponding Viz CoF2 and MnF2 to the applied mechanical stressinproportionate manner.objectives are targeted to capture the pizeomagnetic signals as confirmatory and Pizeoelectric signals induced due to stress applied as preseismic signature in the subsurface of crustal region.

High power lasers for gamma source
Paper 10082-49

Author(s):  Antoine Courjaud, Amplitude Systèmes (France), et al.
Conference 10082: Solid State Lasers XXVI: Technology and Devices
Session 10: Ultrafast Lasers II
Date and Time: Wednesday, February 1, 2017, 2:40 PM

The developments of the ELI-NP interaction lasers are presented. The targeted specifications are 400 mJ @1030 nm, 200 mJ @ 515nm, 100 Hz, 3.5 ps. The laser is composed of an ultrafast oscillator, a regenerative amplifier, a main amplifier, a compressor and second harmonic generation module. A booster may be added to the laser chain in order to meet the targeted pulse duration.

All-fiber widely tunable optical parametric oscillator laser system
Paper 10083-49

Author(s):  Thomas Gottschall, Friedrich-Schiller-Univ. Jena (Germany), et al.
Conference 10083: Fiber Lasers XIV: Technology and Systems
Session 10: Tunable Fiber Laser Sources
Date and Time: Wednesday, February 1, 2017, 2:40 PM

Stimulated Raman Scattering requires an extremely quiet, widely wavelength tunable laser, which, up to now, is unheard of in fiber laser systems. We present a compact all-fiber optical parametric oscillator based on degenerate four-wave mixing (FWM) in an endlessly single-mode photonic crystal fiber, which can be tuned between 785 and 960 nm and 1177 and 1500 nm. Combining FWM based conversion, with all-fiber Yb-based fiber lasers allows for the first extremely compact, turn-key and widely tunable optical parametric oscillator. This source could very well be the missing key instrument to propel CRS imaging to real world applications.

Ultrafast optical reversible Feynman-double logic gate using lithium niobate based Mach Zehnder interferometers
Paper 10105-57

Author(s):  Chanderkanta Chauhan, DIT Univ. (India), et al.
Conference 10105: Oxide-based Materials and Devices VIII
Session PWed: Posters-Wednesday
Date and Time: Wednesday, February 1, 2017, 6:00 PM

In this paper, basic reversible logic gate (double Feynman gate) using the lithium niobate based Mach-Zehnder interferometer is proposed. The results are verified using beam propagation method and MATLAB simulations.

Transmittance through mouse brain tissue with Gaussian and Laguerre Gaussian vortex beams
Paper 10120-14

Author(s):  Lingyan Shi, Columbia Univ. (United States), et al.
Conference 10120: Complex Light and Optical Forces XI
Session PWed: Posters-Wednesday
Date and Time: Wednesday, February 1, 2017, 6:00 PM

Light transmission of Gaussian (G) and Laguerre-Gaussian (LG) vortex beams in mouse brain tissue are investigated. Transmittance is measured with different orbital angular momentum (OAM) at various tissue thickness. In both ballistic and diffusive regions, transmittances of LG and G beams show no significant difference. The transition point from ballistic to diffusive region for the mouse brain tissue is determined at about 480 μm. The observed transmittance of the LG and G beams shows the independence on OAM modes, which may be attributed to brain tissue’s special structures and interference effects.

Design of XOR/AND gate using 2D photonic crystal principle
Paper 10130-25

Author(s):  Lokendra Singh, DIT Univ. (India), et al.
Conference 10130: Next-Generation Optical Communication: Components, Sub-Systems, and Systems VI
Session 8: Advanced Devices and Optical Signal Processing
Date and Time: Thursday, February 2, 2017, 9:50 AM

In this paper, an effective design of all-optical logic gates like XOR gate and AND gate is presented. The structure of these two logic gates is based on T-shape waveguide with optimized silica dielectric rod radius.

Laser-driven parametric generation of coherent THz radiation in graphene and topological insulators
Paper 10123-53

Author(s):  Alexey A. Belyanin, Texas A&M Univ. (United States), et al.
Conference 10123: Novel In-Plane Semiconductor Lasers XVI
Session 12: IR and THz Sources: New Designs II
Date and Time: Thursday, February 2, 2017, 12:00 PM

We show that a strong near-infrared or mid-infrared laser beam obliquely incident on monolayer graphene can experience a parametric instability with respect to decay into lower-frequency (idler) photons and THz surface plasmons. Although graphene is an isotropic medium for low-energy in-plane electron excitations, its second-order nonlinearity is enabled by strong spatial dispersion effects. The parametric gain leads to efficient generation of THz plasmons. Furthermore, the parametric decay process gives rise to quantum entanglement of idler photon and surface plasmon states. A similar parametric process can be implemented with surface states in 3D topological insulators such as Bi2Se3.

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Journal of Biomedical Optics

Journal of Biomedical OpticsPublishes peer-reviewed papers that utilize modern optical technology for improved health care and biomedical research.