Semrock has launched a novel service for Super-resolution Microscopy applications that sets a new standard in performance with guaranteed /2 P-V per inch flatness from 1mm thick laser dichroic beamsplitters mounted in off the shelf, commercially available microscope cubes.
Maximize SNR and minimize artifacts in TIRF, Confocal, PALM, STORM, SIM, and other super-resolution techniques. Offered at attractive pricing, assembled cubes compatible with popular microscopes are now available as standard catalog products.
Conventional microscopy cubes can significantly compromise the flatness of the dichroic beamsplitters thereby introducing aberrations. But super-resolution imaging systems are highly sensitive to optical wavefront distortion and demand the highest quality components for best instrument sensitivity. Semrock's industry-leading λ/2 flatness 1 mm thick laser dichroic beamsplitters minimize focus shift and aberrations in the reflected beam compared to standard products. In order to realize their full flatness potential, these filters need to be carefully mounted in microscopy cubes and Semrock has developed proprietary methods that guarantee the /2 flatness required for super-resolution applications.
Utilizing a 1 mm thick dichroic beamsplitter in the light path minimizes beam deviation, reduces light scatter in the emission path, and enables faster switching speed between different cubes. Semrock's 1 mm thick super-resolution dichroic beamsplitters are designed to minimize reflected wavefront distortion for even large diameter illumination beams and are readily available for most popular microscopy cubes (e.g. Olympus U-MF2 & U-FF, Zeiss FL Cube EC P&C, Nikon TE 2000).