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Intelligent Imaging Innovations, Inc
Company Description
3i designs and manufactures optical microscope systems, components, and software for cutting edge research by customizing microscopy platforms to suit many different needs. Our modular systems can be expanded to address all scientific methods. 3i’s over-arching goal is to continue to create the best imaging technologies by scientists for scientists.
Contact Information
3509 Ringsby Ct.
Denver,CO
United States
Press Releases
| 3i Phasor™ Digital Holographic Photomanipulation
3i offers the first commercial implementation of a phase-only spatial light modulator (SLM) designed specifically for patterned and 3D point photomanipulation in optical microscopy. It is built as a compact, modular device that attaches to one of the microscope’s camera ports and is illuminated by the 3i LaserStack™ through a fiber coupling.
Region specification, hologram generation, and experimentally-synchronized optical path switching and excitation light gating are all managed within 3i’s SlideBook™ software. For over seven years, the SlideBook acquisition engine has enabled sophisticated experiment design incorporating pattern photomanipulation, including FRAP, photoactivation, photoconversion, and photostimulation. Driving Phasor™ is a natural extension to these robust tools.
By using a fiber-coupled laser combiner, it is possible to use Phasor™ with a number of different lasers. With the addition of 3i’s fiber switcher, it is possible to also use those same lasers for other purposes, such as point-scanning photomanipulation, spinning disk confocal imaging, and TIRF illumination. As all switching takes place in a millisecond, it is possible to design very elaborate experiments that leverage the same lasers for a combination of imaging techniques, even at very high speeds.
As a major advantage over systems which block light outside desired regions of interest, the Phasor™ holographic system is able to redirect a significant portion of the illumination intensity to regions where photomanipulation is desired, and therefore can make much more efficient use of the laser power, particularly when regions are small. Digital holography also provides superior optical sectioning for larger regions compared to approaches that use Gaussian beam illumination. Finally, digital holography permits different points of stimulation to be placed on different z planes. Or, alternatively, an entire photomanipulation pattern can be axially displaced from the imaging plane of focus. |
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