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27 - 28-January 2018
San Francisco, CA, United States

Applied Scientific Instrumentation, Inc.

Applied Scientific Instrumentation, Inc.
29391 W Enid Rd
Eugene, OR
United States
Company Description
Featured Product: diSPIM & oSPIM Light sheet microscopes with selective plane illumination

ASI manufactures hardware for microscope automation including: extremely precise motion control devices, such as sub micron XY stages, piezo top plate stages for precise & fast focusing, LED based feedback systems for maintaining focus, High-speed filter wheels. We also build microscope systems including light sheet and other custom high resolution systems. We work with end users as well as OEM’s and offer complete system solutions.
14 August 2017
diSPIM Dual Inverted Selective Illumination Microscope & Oblique Single Plane Illumination Microscope (oSPIM)
ASI’s oSPIM microscope is an excellent platform for high resolution light sheet microscopy for samples mounted in standard coverslip-bottom culture dishes. The oSPIM is a single-view light sheet system where the illumination light sheet is generated at an oblique angle using an oil immersion objective below the sample dish. Fluorescent emission is observed using a high NA water dipping objective from the top side where this objective is tilted 60 degrees, perpendicular to the illumination sheet. The arrangement with high NA objectives both above and below the sample dish allow for high-resolution imaging in a convenient geometry for cell culture work.
The oSPIM is two microscopes in one. The lower microscope can be used for fluorescent imaging using conventional modalities, such as wide field fluorescence, confocal, or TIRF. It also is utilized as the light sheet excitation objective. The tilted top microscope is dedicated to Selective Plane Illumination Microscopy (SPIM) or light sheet images.

ASI’s Dual Inverted Selective Plane Microscopy (diSPIM)

• Use conventional mounting /glass cover slips
• Generate 3D volumes with isotropic resolution (330 nm in all directions)
• Axial resolution is ~2x better than confocal- or spinning disk systems
• Achieve a ~7-10 fold reduction in photobleaching
• Acquisition rates up to 200 images per second or 2-5 volumes per second (roughly 10x faster than spinning-disk
• Compared to Bessel beam plane illumination approaches, diSPIM offers equivalent (or better) axial resolution (~330 nm), 10-100x faster volumetric imaging rates (0.5-1 s instead of 10-100 s), and the ability to image over ~10x more time points (~1000 instead of ~100), due to the significantly lower illumination dose employed in our experiments and the lack of extraneous illumination outside the focal plane
• Microscope has multicolor capability, and has been tested successfully on cells cultured on cover slips, cells embedded in collagen gels, and nematode and zebrafish embryos.
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