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The Moscone Center
San Francisco, California, United States
28 January - 2 February 2017
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Brain Presentations

Clinical Technologies, Laser Tissue Interaction, and Tissue Engineering
(ordered by start date and time)


Investigation of hydrogen sulfide gas using Pd/Pt material based fiber Bragg grating sensor
Paper 10488-8

Author(s):  Amna, DIT Univ. (India), et al.
Conference 10488: Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVIII
Session 3: Sensors Detectors and Treatment Tools II
Date and Time: Saturday, January 27, 2018, 11:30 AM

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In this work, different FBG sensors for detection of hydrogen sulfide gas have been discussed. These designs are based on FBG sensor coated with the pd-material, coated with pt-material and coated with the both material. The devices have been analyzed using different parameters such as measurement angle, sensitivity, selectivity, limit of detection and response time. It is apparent from analysis that sensitivity of hydrogen sulfide gas sensor with pd-material is higher as compared to pt-material. The concentration of hydrogen sulfide gas should be differing from 0 to 100 PPM for proper detection.


Influence of low temperature ageing on optical and mechanical properties of transparent yittria stabilized-zirconia cranial prosthesis
Paper 10486-9

Author(s):  Nami, Univ. of California, Riverside (United States), et al.
Conference 10486: Design and Quality for Biomedical Technologies XI
Session 2: Phantoms and Test Methods
Date and Time: Saturday, January 27, 2018, 11:50 AM

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Laser-based diagnostics and therapeutics show promise for many neurological disorders, but are limited by poor transparency of cranial bone. We addressed this limitation with a novel cranial prosthesis made of transparent nanocrystalline yttria-stabilized-zirconia (nc-YSZ). Although zirconia-based implants have been known for their excellent mechanical properties, the in vivo application was found to be affected by long-term failures, due to low temperature degradation via phase transformation. Following guidelines from ISO 13356:2008 on simulated aging of zirconia, we demonstrate that phase transformation and changes to optical transparency and mechanical properties of nc-YSZ samples are minimal following 25 and 100 h hydrothermal treatments.


Automatical and accurate segmentation of cerebral tissues in fMRI dataset with combination of image processing and deep learning
Paper 10485-9

Author(s):  Zhenglun, Northeastern Univ. (United States), et al.
Conference 10485: Optics and Biophotonics in Low-Resource Settings IV
Session 2: Machine Learning in Imaging, Sensing and Diagnostics
Date and Time: Saturday, January 27, 2018, 12:00 PM

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We proposed to incorporate image processing and deep learning to extract volume values and three-dimensional (3D) morphological info of GM and WM for rapid and precise diagnosis of cerebral atrophy/expansion or leukodystrophy. Such approach greatly reduced the processing time compared to manual and semi-automatic segmentation and is of great importance in improving speed and accuracy by more samples deep learned. Plus the contours of the borders of GM and WM on all images were accurately extracted and 3D visualized, which offered doctors and researchers quantitative volume data and detailed morphological characterization for personal precise medicine of Cerebral atrophy/expansion. The whole software was developed as mobile app, which is applicable in both clinics and personal health care.


Measurements of coherent hemodynamics to enrich the physiological information provided by near-infrared spectroscopy (NIRS) and functional MRI
Paper 10487-13

Author(s):  Angelo, Tufts Univ. (United States), et al.
Conference 10487: Multimodal Biomedical Imaging XIII
Session 3: Clinical Applications
Date and Time: Saturday, January 27, 2018, 2:10 PM

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We describe how coherent hemodynamics may enhance the functional and physiological information provided by NIRS and fMRI. A technique that we recently introduced, Coherent Hemodynamics Spectroscopy (CHS), relies on measurements of hemodynamic changes that are coherent with a physiological quantity of interest (for example, mean arterial pressure). We describe measures of coherence and the minimum acceptable level of coherence, which depends on the specific application. We report some application of CHS to absolute measurements of cerebral blood flow and autoregulation, and we characterize the dynamic relationship between mean arterial pressure and blood volume in human subjects.


Laser speckle imaging of brain blood flow through a transparent nanocrystalline yttria-stabilized-zirconia cranial implant
Paper 10493-2

Author(s):  Nami, Univ. of California, Riverside (United States), et al.
Conference 10493: Dynamics and Fluctuations in Biomedical Photonics XV
Session 1: Speckle Technologies
Date and Time: Sunday, January 28, 2018, 9:10 AM

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Laser speckle flowmetry methods based on laser speckle imaging (LSI) allow for brain blood flow visualization with high spatiotemporal resolution. However, the poor transparency of the cranial bone limits the spatial resolution and imaging depth. Our study addresses these limitations with a transparent nanocrystalline yttria-stabilized zirconia (nc-YSZ) cranial implant. We demonstrate that spatial resolution and quantitative accuracy of blood flow measurements are improved when LSI is conducted through the implant rather than native skull in an acute murine model. These results demonstrate the feasibility of nc-YSZ implants to provide clinically-viable long-term optical access for LSI on a chronically-recurring basis.


Design and analysis of FBG based sensor for detection of damage in oil and gas pipelines for safety of marine life
Paper 10488-31

Author(s):  Amna, DIT Univ. (India), et al.
Conference 10488: Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVIII
Session 8: Sensors Detectors and Treatment Tools VII
Date and Time: Sunday, January 28, 2018, 1:20 PM

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The underwater pipelines structures are laid on the seafloor for transportation of oil and gas from the sub-sea structures to the various parts of the world. For timely detection of damages, a new technique based on laying an array of FBG along the pipelines for detection of oil and gas leakage caused by damage arising due to environmental hazard has been proposed. The numerical calculation based on the acoustic emission generated by defect in pipeline has been studied based on the fundamental of strain measurement and the output has been simulated using MATLAB.


In-vivo assessment of radiation-induced cerebrovascular damage in mice by hybrid optoacoustic-ultrasound bio-microscopy
Paper 10494-17

Author(s):  Johannes, Helmholtz Zentrum München GmbH (Germany), et al.
Conference 10494: Photons Plus Ultrasound: Imaging and Sensing 2018
Session 3: Therapy Monitoring and Guidance II
Date and Time: Sunday, January 28, 2018, 2:00 PM

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Ionizing radiation is used for the treatment of primary and metastatic brain tumors yet may result in a number of side effects, such as radiation-induced damage to the vascular network, which could ultimately lead to white matter necrosis. The mechanisms inducing the damage at a cellular and molecular level are poorly understood and the current imaging techniques are not suitable for accurate evaluation of the radiation-induced alterations in the micro-vasculature in-vivo. Here we use a hybrid optoacoustic and ultrasound bio-microscopy system in order to reveal damage to the skull and brain vasculature of mice in-vivo caused by ionizing radiation. The system is capable of rapid transcranial visualization of large-scale cerebral vascular networks and offers three-dimensional views of the intricate vascular networks with single capillary resolution and field of view of more than 6x8 mm² covering the entire cortical vasculature in mice. The 3D nature of the acquired optoacoustic images combined with a clear anatomical reference of the pulse-echo ultrasound data allowed clear differentiation of the skull vasculature from the superficial brain vasculature. The irradiation was selectively applied to one brain hemisphere and the effects of the ionizing radiation were evident without introduction of extrinsic labeling, owing to the strong hemoglobin-based contrast of optoacoustics.


Integrated Raman biopsy probe for high yield targeted brain cancer biopsies
Paper 10484-12

Author(s):  Joannie, Ecole Polytechnique de Montréal (Canada), et al.
Conference 10484: Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XVI
Session 3: Robotics and Light Biopsies for Surgical Applications
Date and Time: Sunday, January 28, 2018, 2:50 PM

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We present an innovative device to improve diagnostic yield associated with brain needle biopsies. The new intraoperative cancer detection optical needle system is based on the combination of fingerprint and high wavenumber Raman spectroscopy for highly accurate brain biopsy targeting based on molecular tissue features. It integrates a micro-probe directly into the internal cannula of a commercial brain biopsy needle. We report on the design, development, and validation of this in situ intraoperative, label-free, cancer detection system and present the results of the first use of this integrated optical cancer detection system in 5 patients during brain biopsy procedures.


Structure-stiffness relation of the brain tissue determined by oscillatory indentation mapping
Paper 10496-39

Author(s):  Nelda, Vrije Univ. Amsterdam (Netherlands), et al.
Conference 10496: Optical Elastography and Tissue Biomechanics V
Session 9: Tissue Mechanical Properties
Date and Time: Sunday, January 28, 2018, 3:45 PM

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To investigate the structure-stiffness relation of brain tissue we have conducted dynamic indentation-controlled mapping on isolated horizontal mouse brain sections. We report highly heterogeneous viscoelasticity maps of hippocampus where regions with specific mechanical properties correlate with structurally distinct layers. By estimating the mean stiffness and the density of nuclei of eleven anatomical regions, we found that more densely packed cell layers have lower stiffness. Our study presents a novel indentation method able to map viscoelastic properties at a high spatial resolution, which leads to a better understanding of the relation between brain tissue composition and stiffness in physiological and pathological conditions.


3D on-chip microscopy of optically cleared tissue
Paper 10485-35

Author(s):  Yibo, Univ. of California, Los Angeles (United States), et al.
Conference 10485: Optics and Biophotonics in Low-Resource Settings IV
Session 8: Computational Imaging and Sensing
Date and Time: Sunday, January 28, 2018, 4:00 PM

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Low-cost tissue clearing techniques, such as the simplified CLARITY method (SCM), are promising to potentially reduce the cost of disease diagnosis by providing 3D imaging/phenotyping of thicker tissue samples with simpler preparation steps. Here we demonstrate 3D imaging of SCM-cleared tissue using on-chip holography, which is based on pixel-super-resolution and multi-height phase-recovery algorithms to compute the sample’s amplitude/phase images at various depths. With this computational approach, we achieved 3D imaging of a 0.2mm thick cleared mouse brain tissue over >20mm2 field-of-view. Moreover, the lens-free microscope achieves ~10-fold improved data efficiency compared to its lens-based counterparts for volumetric imaging of samples.


Non-invasive detection of matrix-metalloproteinase activity in a mouse model of cerebral ischemia using multispectral optoacoustic tomography
Paper 10494-23

Author(s):  Ruiqing, ETH Zürich (Switzerland), et al.
Conference 10494: Photons Plus Ultrasound: Imaging and Sensing 2018
Session 4: Preclinical Imaging
Date and Time: Sunday, January 28, 2018, 4:15 PM

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Matrix metalloproteinases (MMPs) play important roles in the pathophysiology of cerebral ischemia. Here we visualized in vivo MMP activity in the transient middle cerebral artery occlusion (tMCAO) mouse model in the ischemic lesion using multispectral optoacoustic imaging (MSOT) and an MMP-activatable probe. MSOT data was coregistered with structural magnetic resonance imaging (MRI) obtained at 7T for localization of signal distribution. We demonstrated co-localization of upregulated MMP signal within the focal ischemic lesion in the tMCAO mouse model using MSOT/MRI multimodal imaging.


Design of tapered optical fibers to achieve high spatial selectivity during infrared neural stimulation
Paper 10488-39

Author(s):  Nelson, Univ. of Miami (United States), et al.
Conference 10488: Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVIII
Session 9: Sensors Detectors and Treatment Tools VIII
Date and Time: Sunday, January 28, 2018, 4:30 PM

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The purpose of this study is to determine if tapered fibers are capable of delivering focused infrared radiation to induce neural stimulation (INS). The accuracy of a numerical simulation program developed to predict the tapered fiber emittance was determined by comparing predicted output to measurements made at multiple energy levels and distances away from a custom-designed tapered fiber (core diameter = 200µm, tapered output face = 50µm, NA=0.22). The predicted values matched experimental values and suggest the feasibility of tapered optical fibers in achieving highly, spatially selective INS.


Which experimental model can sensitively indicate brain death by functional near-infrared spectroscopy?
Paper 10484-42

Author(s):  Boan, Univ. of Electronic Science and Technology of China (China), et al.
Conference 10484: Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XVI
Session Sun: Poster Session
Date and Time: Sunday, January 28, 2018, 5:30 PM

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Brain death is defined as permanent loss of the brain functions. The diagnosis of it has many meanings. The standard clinical tests are expensive, time consuming and even dangerous, and some auxiliary methods have limitations. Here, we attempt to use our FNIRS to diagnose brain death and find which experimental mode is effective. They were provided different fractional concentration of inspired oxygen (FIO2) in different phase. We found that the ratio of Δ[HbO2] (the concentration changes in oxyhemoglobin) to Δ[Hb] (the concentration changes in deoxyhemoglobin) in brain death patients is significantly higher than normal patients in FIO2 experiment. Combined with the data analysis result, restore oxygen change process and low-high-low paradigm is more sensitively.


Hyperspectral near infrared spectroscopy assessment of the brain during hypoperfusion
Paper 10484-43

Author(s):  Thu Nga, Ryerson Univ. (Canada), et al.
Conference 10484: Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XVI
Session Sun: Poster Session
Date and Time: Sunday, January 28, 2018, 5:30 PM

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Post-cardiac arrest brain injury is a common cause of death after cardiac arrest, yet there is no accepted approach to monitor cerebral perfusion and oxygenation during cardiac arrest resuscitation. In this study, we use the hyperspectral near infrared spectroscopy (hNIRS) to monitor acute changes in both hemoglobin and cytochrome C oxidase of the brain in patients undergoing transcatheter aortic valve insertion - a procedure that mimics cardiac arrest. We found that while measured rSO2 changes resulted mainly from the extra-cerebral tissues, CCO changes occurred mainly in the brain. To improve the quantitative accuracy of measurements we applied the 2-layer algorithm to hNIRS data.


Cost effective wireless embedded multichannel NIRS system
Paper 10485-45

Author(s):  Chuan-Hsiang, National Chiao Tung Univ. (Taiwan), et al.
Conference 10485: Optics and Biophotonics in Low-Resource Settings IV
Session Sun: Poster Session
Date and Time: Sunday, January 28, 2018, 5:30 PM

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Near-infrared spectroscopy (NIRS) system is recently a useful and popular technique for therapeutic applications. It detects the optical attenuation in tissues and derive relative oxyhemoglobin (HbO2) and deoxyhemoglobin (Hb) at discrete wavelengths. Compared with other commercial instruments our wearable NIRS system is quite cheap, but it can also do the most jobs that commercial instruments can do. Our system powered by two 3.7v chargeable Li batteries and have four channels in total covering the prefrontal cortex. With the homemade software, the application of our system can be more flexible to deal with most kind of cases. We test our system with mental algorithm (serial subtraction of 1000-13). Early researches said that subjects’ prefrontal cortex will get activated during the mental algorithm task. And by comparing the NIRS and fMRI data to verify our system.


Monitoring of acute injury in experimental cerebral ischemic stroke using optical coherence tomography
Paper 10493-18

Author(s):  Woo June, Univ. of Washington (United States), et al.
Conference 10493: Dynamics and Fluctuations in Biomedical Photonics XV
Session PSun: Posters-Sunday
Date and Time: Sunday, January 28, 2018, 5:30 PM

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We introduce microscopic optical imaging methods that monitor brain injury progression using intrinsic optical scattering properties of cerebral cortex in vivo. An optical coherence tomography (OCT) system is used for imaging of local ischemic stroke in mice with open-skull cranial window surgery preparations. After onset of the ischemic insult, our imaging methods on OCT platform visualize and quantify microscopic cascade events including rarefaction of capillary perfusion, cerebral blood flow deficiency, altered cellular scattering and deformed vascular geometry in the ischemic region of cortex that are featured within a few hours of post stroke.


Identification of the patterns of brain activity during the imagination of movements using an artificial neural network
Paper 10493-63

Author(s):  Semen, Saratov State Technical Univ. (Russian Federation), et al.
Conference 10493: Dynamics and Fluctuations in Biomedical Photonics XV
Session PSun: Posters-Sunday
Date and Time: Sunday, January 28, 2018, 5:30 PM

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Recognition and classification of EEG patterns of real and imaginary motion were carried out on the basis of data obtained from a group of volunteers. A post-processing approach was used and EEG signals were divided into fragments corresponding to different periods of the experiment: real or imaginary left or right hand/foot movement, fragments of different background activity. Classification was carried out by the method of artificial neural networks (ANN). Classification of various movements of the left and right legs shows good results. The best recognition level is 85-89%.


OCT Eigen decompensation based penetrating vessel mapping
Paper 10493-15

Author(s):  Wei, Univ. of Washington (United States), et al.
Conference 10493: Dynamics and Fluctuations in Biomedical Photonics XV
Session 4: Functional Imaging and Spectroscopy I
Date and Time: Sunday, January 28, 2018, 5:35 PM

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Penetrating vessels bridge the mesh of communicating vessels on the surface of cortex with the subsurface microvascular bed that feeds the underlying neural tissue. We propose a cerebral penetrating vessel mapping approach based on eigen decompensation (ED) principle component analysis that is innovatively redesigned from optical coherence tomography (OCT) angiography. The statistical analysis is immune to tailing artifacts, enabling automatic decoupling of penetrating vessels from lateral vasculature networks. This method allows for statistical quantification of penetrating arterioles and ascending venules from large volume OCT angiography datasets, and accordingly contributes to the morphometric analysis of cortical microvasculature in functioning brains.


Combined NIRS and DCS measurements of cerebral hemodynamics during intracranial and blood pressure changes
Paper 10493-16

Author(s):  Alexander, Carnegie Mellon Univ. (United States), et al.
Conference 10493: Dynamics and Fluctuations in Biomedical Photonics XV
Session 5: Cerebral Hemo- Lympho- and Glymphatic Dynamics
Date and Time: Monday, January 29, 2018, 8:20 AM

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We have recently shown that near-infrared spectroscopy (NIRS) measurements of hemoglobin concertation changes can be correlated to cerebral perfusion pressure in non-human primates, demonstrating the possibility of non-invasive intracranial pressure monitoring. Here we present on an extended study, where we combined NIRS with diffuse correlation spectroscopy (DCS) to obtain cerebral blood flow measures, allowing quantification of cerebral autoregulation. We will present on the experimental design and data analysis approaches for data collected on non-human primates during exsanguination.


OCT angiography reveals age-related differences in cerebral blood flow of anesthetized mice
Paper 10493-19

Author(s):  Woo June, Univ. of Washington (United States), et al.
Conference 10493: Dynamics and Fluctuations in Biomedical Photonics XV
Session 5: Cerebral Hemo- Lympho- and Glymphatic Dynamics
Date and Time: Monday, January 29, 2018, 9:30 AM

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We examine the effect of aging on cerebral microcirculation up to a capillary flow scale. This study uses optical coherence tomography angiography (OCTA) to measure vessel tortuosity, red blood cell (RBC) speed in individual capillaries and capillary density in the sensory-motor cortex of 8 young (3-month-old) and 8 aged (16-month-old) mice under isoflurane anesthesia. The result shows that the surface arterial vessels are more tortuous and the capillary RBC speed is much higher in aged animals old compared with young ones. However, the capillary vessel density is significantly lowered in the aged group than the young group.


Evaluating vasculature changes in the murine embryonic brain due to prenatal alcohol exposure using optical coherence tomography, in utero
Paper 10493-20

Author(s):  Raksha, Univ. of Houston (United States), et al.
Conference 10493: Dynamics and Fluctuations in Biomedical Photonics XV
Session 5: Cerebral Hemo- Lympho- and Glymphatic Dynamics
Date and Time: Monday, January 29, 2018, 9:50 AM

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Fetal alcohol spectrum disorders refers to a spectrum of abnormalities associated with prenatal alcohol exposure (PAE). Volume of alcohol consumed and period of gestation during which exposure occurred influence the severity of the abnormality. Although changes in blood flow caused by PAE during the second trimester has been well documented, acute vasculature changes in the brain have not been evaluated. This work uses speckle variance optical coherence tomography (SVOCT) to evaluate changes in murine fetal brain vasculature, in utero, minutes after maternal alcohol consumption. Results showed a rapid and significant decrease (p<0.001) in vessel diameter as compared to sham group.


OCT velocimetry reveals electrical-evoked temporal capillary hemodynamics in mouse cerebral cortex during functional activation
Paper 10493-21

Author(s):  Wei, Univ. of Washington (United States), et al.
Conference 10493: Dynamics and Fluctuations in Biomedical Photonics XV
Session 5: Cerebral Hemo- Lympho- and Glymphatic Dynamics
Date and Time: Monday, January 29, 2018, 10:10 AM

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The cerebral vascular system serves constant demand of neuronal activities in the brain. Temporal dynamics and spatial redistribution of red blood cells (RBC) within the capillary bed play a deterministic role in oxygen diffusing capacity. Taking the advantages of the high spatiotemporal resolution of OCT velocimetry designed upon eigen-decomposition (ED) statistical analysis, we investigated the intrinsic capillary RBC fluctuations within mouse cerebral cortex, representing as bandwidths of the RBC flow frequencies. Neural activation in the hindpaw somatosensory cortex evokes spatiotemporal redistribution of capillary hemodynamics regulated through instantaneous increments in flow disturbance and flow velocity, but involves no recruitment of reserved capillaries (no RBC transit path variation).


Quantification of amyloid deposits and oxygen extraction fraction in the brain with multispectral optoacoustic imaging in arcAbeta mouse model of Alzheimer's disease
Paper 10494-50

Author(s):  Ruiqing, ETH Zurich (Switzerland), et al.
Conference 10494: Photons Plus Ultrasound: Imaging and Sensing 2018
Session 8: Functional Imaging and Brain Imaging
Date and Time: Monday, January 29, 2018, 3:30 PM

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The interaction of beta-amyloid (Aβ) deposition and vascular dysfunction are important pathophysiologies in Alzheimer’s disease (AD). Planar optical imaging such as near-infrared (NIRF) is limited by spatial resolution and differentiation of signal from different tissue compartment. Here we provide novel non-invasive in-vivo estimates of brain Aβ load with Aβ-binding probe CRANAD-2 and the brain oxygen-extraction-fraction (OEF) by using multi-spectral optoacoustic imaging (MSOT) in arcAβ mouse models of AD at 24-month of age. We demonstrated increased cerebral OEF and cortical amyloid load in arcAβ compared to wild-type mice.


Transcranial recording of stimulated neuronal activity in vivo using photoacoustic voltage-sensitive dye imaging
Paper 10494-53

Author(s):  Jeeun, Johns Hopkins Univ. (United States), et al.
Conference 10494: Photons Plus Ultrasound: Imaging and Sensing 2018
Session 8: Functional Imaging and Brain Imaging
Date and Time: Monday, January 29, 2018, 4:15 PM

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Non-invasive monitoring of electrophysiological brain activities in real-time has been demanded in neuroimaging field. Here we present in vivo proof-of-concept results for non-invasive sensing of neuronal activities in rat brain by using transcranial photoacoustic imaging of near-infrared voltage-sensitive dye (VSD). Using the frequency-domain signal analysis on temporal photoacoustic sequence, the amount and firing frequency of brain activity have successfully quantified in vivo. These studies demonstrate that photoacoustic imaging of fluorescence quenching-based VSD is a powerful tool for recording deep brain activities of rat brain without any invasive craniotomy.


Effects of short infrared laser pulses on neuron metabolism
Paper 10492-17

Author(s):  Roxanne, Georgia Institute of Technology (United States), et al.
Conference 10492: Optical Interactions with Tissue and Cells XXIX
Session 4: Laser Modulation of Cells
Date and Time: Monday, January 29, 2018, 4:30 PM

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Pulsed infrared light is capable of both activating and reversibly blocking action potential in neurons. Given its high tenability and precise delivery, short infrared laser pulse (SILP) therapy is a promising strategy for neuromodulation. We hypothesize that SILP causes short term changes in metabolism. To test this hypothesis, we are probing the effects of SILP on neuron metabolism using optical metabolic imaging (OMI). Cells will be stimulated with a single millisecond pulse or pulse train of 1869 nm light, and NADH and FAD fluorescence intensity and fluorescence lifetime will be imaged before and after SILP.


DAS: A simple, efficient, scalable and DiI-compatible optical clearing method for intact systems
Paper 10493-33

Author(s):  Lingling, Shenzhen Univ. (China), et al.
Conference 10493: Dynamics and Fluctuations in Biomedical Photonics XV
Session 8: Optical Clearing and Biomechanics
Date and Time: Monday, January 29, 2018, 5:00 PM

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There is an increasing interest in the three-dimensional visualization and quantification of cellular circuits in the brain and therefore optical clearing methods are highly in demand for brain imaging. In particular, clarification without membrane damage is required to image lipophilic tracer-labeled neural tracts. However, previously reported DiI-compatible optical clearing methods (i.e. SeeDB, FRUIT and ScaleS) are slow and can hinder transparency for imaging. Here, we present DAS, a new, convenient and reproducible optical clearing method that can efficiently clarify large tissues without volume enlargement phase and reliably preserves emission from fluorescent proteins and lipophilic dyes in membrane integrity preserved tissues.


Capillary red blood cell velocimetry by phase-resolved optical coherence tomography
Paper 10483-105

Author(s):  Jianbo, Massachusetts General Hospital (United States), et al.
Conference 10483: Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXII
Session Mon: Poster Session II: Functional and Applications
Date and Time: Monday, January 29, 2018, 5:30 PM

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Approaching through-skull optical brain imaging and phototherapy: optical properties of head tissues in near and short-wave infrared regions
Paper 10492-37

Author(s):  Sergii, Shenzhen Univ. (China), et al.
Conference 10492: Optical Interactions with Tissue and Cells XXIX
Session PMon: Posters-Monday
Date and Time: Monday, January 29, 2018, 5:30 PM

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The optical properties of the rat head tissues: as brain cortex, cranial bone and skin has been assessed in the visible, near-infrared and short-wave infrared regions (350-2800 nm), aiming towards the trough-skull light applications, such as brain imaging and phototherapy. Four transparence windows have been identified for head tissues: 700-1000 nm, 1000-1350 nm, 1550-1870 nm and 2100-2300 nm. The possibilities to exploit these windows for trough-skull optical imaging and phototherapy are considered.


Real-time volumetric mapping of calcium activity in living mice by functional optoacoustic neuro-tomography
Paper 10494-52

Author(s):  Sven, Institut für Biologische und Medizinische Bildgebung, Helmholtz Zentrum München GmbH (Germany), et al.
Conference 10494: Photons Plus Ultrasound: Imaging and Sensing 2018
Session 8: Functional Imaging and Brain Imaging
Date and Time: Monday, January 29, 2018, 5:30 PM

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Here we demonstrate that functional optoacoustic neuro-tomography (FONT) enables non-invasive imaging of sensory-evoked brain activity in living mice expressing genetically encoded calcium indicator (GECI). We applied a somatosensory electrical hindpaw stimulation protocol and observed rapid optoacoustic signal transients in the activated brain regions but not inside major vasculature, allowing for a clear differentiation of the calcium-related activity from the underlying hemodynamic responses. Our study is the first to examine fast optoacoustic signatures of GECIs non-invasively in living mice, paving the way for large-scale neural recording at penetration depths and spatio-temporal resolution scales not covered with the existing neuroimaging techniques.


Comprehensive photoacoustic characterization of the cerebral vasculature in awake mice
Paper 10494-161

Author(s):  Rui, Univ. of Virginia (United States), et al.
Conference 10494: Photons Plus Ultrasound: Imaging and Sensing 2018
Session PMon: Posters-Monday
Date and Time: Monday, January 29, 2018, 5:30 PM

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Increasing evidence shows that cerebrovascular dysfunction plays an important role in a variety of brain disorders, including traumatic brain injury, stroke, and Alzheimer’s disease. However, mechanistic studies of cerebrovascular dysfunction in awake mice without the influence of anesthesia remains challenging, due to the lack of proper imaging tools. We have developed a set of experimental and analytical protocols for comprehensive characterization of the cerebral vasculature (including vessel diameter, tortuosity, density, concentration and oxygen saturation of hemoglobin, blood flow, resistance, wall shear stress, reactivity, and the permeability of blood brain barrier) and how they go awry in diseases using head-restrained PAM.


The study on fast localization method of anomaly block in brain based on differential optical density
Paper 10484-39

Author(s):  Huiquan, Tianjin Polytechnic Univ. (China), et al.
Conference 10484: Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XVI
Session 10: Imaging: Theory and Simulations
Date and Time: Tuesday, January 30, 2018, 11:40 AM

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In vivo, noncontact, real-time, optical and spectroscopic assessment of the immediate local physiological response to spinal cord injury in a rat model
Paper 10489-10

Author(s):  Joseph, Syracuse Univ. (United States), et al.
Conference 10489: Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis
Session 3: Spectroscopic Instrumentation and Designs
Date and Time: Tuesday, January 30, 2018, 1:20 PM

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We report results of a small study intended to test a methodology for real-time detection and imaging of chemical and physical changes in spinal cords immediately after a localized, moderate contusive injury. Raman spectroscopy, optical profilometry and scanning NIR autofluorescence images were utilized simultaneously in vivo. Only injured cords display a Raman feature that could indicate extensive, localized protein phosphorylation may occur minutes following spinal cord trauma. We believe these results justify exploration of this approach to the study of spinal cord injury, traumatic brain trauma and possibly other forms of CNS damage.


Photoacoustic sensing of bio-electrical activity using quantum dots
Paper 10494-80

Author(s):  Nashaat, George Mason Univ. (United States), et al.
Conference 10494: Photons Plus Ultrasound: Imaging and Sensing 2018
Session 12: Molecular Imaging
Date and Time: Tuesday, January 30, 2018, 2:45 PM

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This study investigated the feasibility of photoacoustic imaging (PAI) of biopotentials, which relies on absorption of light by voltage-sensitive probes and subsequent generation/detection of ultrasound. PC12 cells were tagged with a voltage-sensing construct that consisted of a photoluminescent quantum dot (QD) anchored to the cell-lipid bilayer using a peptide-C60 anchor. Labeled cells were depolarized by administering 10 mM potassium chloride (KCl). The resulting change in cell-membrane potential (~35 mV) was verified using whole-cell patch-clamp. A 532-nm laser excited the photoacoustic (PA) response from QD-labelled cells. After administering KCl, the PA-signal increased by 48%, which corresponds to 1.5% per mV.


Measuring 3D temperature profiles using phase-decorrelation OCT
Paper 10483-50

Author(s):  Junqi, Case Western Reserve Univ. (United States), et al.
Conference 10483: Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXII
Session 8: OCT New Technology II
Date and Time: Tuesday, January 30, 2018, 4:15 PM

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Accurately measuring temperature profiles inside tissue can be challenging. We have developed a phase-decorrelation OCT method to measure 3D temperature profiles. We measured phase decorrelation in liquid and solid phantoms. As the laser power was increased, phase decorrelation was linearly proportional to temperature measurements made with a thermocouple and thermal camera. We are particularly interested in this technique for improving targeting and assessing safety of IR neuromodulation protocols, but measuring 3D temperature profiles with high resolution is likely to have many other applications.


Few-mode fiber OCT for angular scattering contrast in tissue
Paper 10483-77

Author(s):  Pablo, Medizinische Univ. Wien (Austria), et al.
Conference 10483: Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXII
Session 12: Novel Contrast
Date and Time: Wednesday, January 31, 2018, 4:00 PM

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A few-mode fiber OCT system has been developed for characterizing the angular scattering of tissue samples. The capability of few-mode fibers for delivering light through different path-lengths depending on the angle of excitation was investigated for OCT applications. Depending on the incident angle into the fiber, the beam will travel different paths, thereby reconstructing the modal information at different depths of the image. After a systematic investigation of the modal intensity dependence on the angle of reflection, the system was demonstrated for detecting amyloid-beta plaques formed in brains affected by Alzheimer’s disease.


Noninvasive measurement of cerebral venous oxygenation in neonates with a multi-wavelength, fiber-coupled laser diode optoacoustic system
Paper 10494-9

Author(s):  Stephen, The Univ. of Texas Medical Branch (United States), et al.
Conference 10494: Photons Plus Ultrasound: Imaging and Sensing 2018
Session 1: Clinical Applications
Date and Time: Sunday, January 28, 2018, 10:00 AM

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Noninvasive measurement of neonatal cerebral venous oxygenation could provide critical information on cerebral hypoxia. By probing the superior sagittal sinus (SSS), a large central cerebral vein, we can measure oxygenation with our multi-wavelength, fiber-coupled laser-diode optoacoustic system. SSS oxygenation was measured in reflection mode through open anterior and posterior fontanelles without obscuration by the overlying calvarium. In transmission mode it was measured through the skull in the occipital area. We monitored the SSS oxygenation in neonates with varying gestation, birth weight and clinical histories to identify normal range and difference between neonates with and without risk factors for cerebral hypoxia.


Micro-engineering a novel platform to reconstruct physiology and functionality of the human brain microvasculature in vitro
Paper 10491-23

Author(s):  Yasaman, Univ. of California, Berkeley (United States), et al.
Conference 10491: Microfluidics, BioMEMS, and Medical Microsystems XVI
Session 5: Applications II
Date and Time: Sunday, January 28, 2018, 3:00 PM

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We introduce a new fabrication method for mimicking 3D multi-layered brain vasculature in vitro. Our technique involves a sequence of hydrogel casting and thermal gelation steps using a coaxial micromolding platform. The method supports the culture of multiple cell types including human astrocytes, pericytes, and brain endothelial cells. The platform eliminates rigid polymeric surfaces from the cell surroundings and thus provides a physiologically relevant mechanical microenvironment. Our model offers spatially heterogeneous elastic moduli and enables co-culturing of multiple types and densities of cells. The technique could be applied to fundamental studies of the blood-brain barrier and to pharmaceutical development.


Fabrication of an optically-penetrating, multi-layered organ-on-chip device to study longitudinal changes in blood-brain barrier optical redox ratios
Paper 10491-42

Author(s):  Nasya, Univ. of Arkansas (United States), et al.
Conference 10491: Microfluidics, BioMEMS, and Medical Microsystems XVI
Session 10: Medical Microsystems II
Date and Time: Monday, January 29, 2018, 4:00 PM

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In this paper, we engineered and used a blood-brain barrier-on-chip system in conjunction with multiphoton microscopy to observe longitudinal changes in cell morphology and metabolic activity, via optical redox imaging, following a traumatic brain injury (TBI). Optical redox ratios were calculated using averaged and normalized two-photon autofluorescence intensities of nicotinamide dinucleotide (NADH) and flavin adenine dinucleotide (FAD) images, as FAD/(NADH+FAD). Our results demonstrated, via live multiphoton microscopy, acute cellular swelling in the glial cells 24 hours post-TBI. Optical redox ratios were reduced with TBI, and current work is ongoing to assess time-dependent changes in cell optical redox ratio following TBI.


Noninvasive optoacoustic monitoring of cerebral venous blood oxygenation in humans: Validation with invasive measurements
Paper 10494-54

Author(s):  Yuriy, The Univ. of Texas Medical Branch (United States), et al.
Conference 10494: Photons Plus Ultrasound: Imaging and Sensing 2018
Session 8: Functional Imaging and Brain Imaging
Date and Time: Monday, January 29, 2018, 4:30 PM

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Management of TBI patients requires cerebral venous oxygenation measurements, currently by invasive techniques. Recently we developed transmission-mode optoacoustic systems for this application by probing the superior sagittal sinus (SSS), a large central cerebral vein. In this study we validated our optoacoustic SSS oxygenation measurements in adult TBI patients using invasive measurements with jugular bulb catheters. Multi-wavelength optoacoustic systems operating in the NIR spectral range were used for measurements. The obtained results demonstrated high correlation of the noninvasive, optoacoustic SSS oxygenation measurements with the invasive measurements. Bland-Altman analysis yielded clinically acceptable bias and standard deviation of -2.7% and 3.5%, respectively.


A fast MEMS scanning photoacoustic microscopy system and its application in glioma study
Paper 10494-88

Author(s):  Renzhe, Singapore Bioimaging Consortium (Singapore), et al.
Conference 10494: Photons Plus Ultrasound: Imaging and Sensing 2018
Session 13: Microscopy
Date and Time: Wednesday, January 31, 2018, 8:00 AM

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We present a MEMS based scanning photoacoustic microscopy (PAM) system and its application in glioma mouse model study. Our PAM system has high optical resolution (~2.5 μm) and high scanning speed (up to 50 kHz), which is ideal for cerebral vascular imaging. In this study, the mice with glioma are treated with vascular disrupting agent (VDA). Our PAM system is utilized to image the cerebral with the whole skull intact before and after the injection of VDA. By image registration, the response of every single blood vessel can be traced. This will provide us deeper understanding of the drug effect.


Control of epileptic seizures in WAG/Rij Rats by means of brain-computer interface
Paper 10493-34

Author(s):  Vladimir V. , Saratov State Univ. (Russian Federation), et al.
Conference 10493: Dynamics and Fluctuations in Biomedical Photonics XV
Session PSun: Posters-Sunday
Date and Time: Sunday, January 28, 2018, 5:30 PM

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We developed an algorithm for predicting seizures in real time, evaluated it and implemented it into an online closed-loop brain stimulation system designed to prevent typical for the absence of epilepsy of spike waves (SWD) in the genetic rat model. The algorithm correctly predicts more than 85% of the seizures and the rest were successfully detected. A large number of false positive occurred during a light slow wave sleep. The inclusion of criteria to prevent false positives greatly reduced the rate of false alarms. However it reduced the sensitivity of the algorithm. We introduced the latest version of BCI into a closed system of stimulation of the brain, which led to a reduction in seizure activity by 70%. Unlike the old beliefs that SWDs are unpredictable, current results show that they can be predicted and that the development of systems for predicting and preventing closed-loop capture is a feasible step on the way to intervention to achieve control and freedom from epileptic seizures.


Nonlinear dynamics and coherent resonance in a network of coupled neural-like oscillators
Paper 10493-40

Author(s):  Andrei V. , Yuri Gagarin State Technical Univ. of Saratov (Russian Federation), et al.
Conference 10493: Dynamics and Fluctuations in Biomedical Photonics XV
Session PSun: Posters-Sunday
Date and Time: Sunday, January 28, 2018, 5:30 PM

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We analyze nonlinear dynamics of coupled neural-like oscillators network some of which are stimulated by external stimulus. We find phenomenon of coherence resonance in it. We find that the regularity in spiking behavior of that stimulated neural network maximizes at a certain level of environment noise. The network coherence is also maximized at a certain stimulus amplitude, a network size, and a number of stimulated neurons. The coherence enhancement is characterized by the signal-to-noise ratio calculated from the average power spectra of the neural network, autocorrelation time, and the normalized standard deviation of the average spiking amplitude.


The study of human higher mental functions as they relate to neurophysiological processes and personal characteristics
Paper 10493-42

Author(s):  Anastasiya E. , Saratov State Technical Univ. (Russian Federation), et al.
Conference 10493: Dynamics and Fluctuations in Biomedical Photonics XV
Session PSun: Posters-Sunday
Date and Time: Sunday, January 28, 2018, 5:30 PM

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Brain-computer interface for alertness estimation and improving
Paper 10493-48

Author(s):  Alexander E. , Yuri Gagarin State Technical Univ. of Saratov (Russian Federation), et al.
Conference 10493: Dynamics and Fluctuations in Biomedical Photonics XV
Session PSun: Posters-Sunday
Date and Time: Sunday, January 28, 2018, 5:30 PM

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Using wavelet analysis of EEG, we study the brain activity associated with perception of visual stimuli. We demonstrate that the brain can process visual stimuli in two scenarios: (i) perception is characterized by destruction of the alpha-waves and increase in the high-frequency (beta) activity, (ii) the beta-rhythm is not well pronounced, while the alpha-wave energy remains unchanged. The special experiments show that the motivation factor initiates the first scenario, explained by the increasing alertness. Based on the obtained results we build the brain-computer interface and demonstrate how the degree of the alertness can be estimated and controlled in real experiment.


Nonlinear correlation method for the separation of couplings in EEG experiments with neural ensembles
Paper 10493-49

Author(s):  Alexander E. , Yuri Gagarin State Technical Univ. of Saratov (Russian Federation), et al.
Conference 10493: Dynamics and Fluctuations in Biomedical Photonics XV
Session PSun: Posters-Sunday
Date and Time: Sunday, January 28, 2018, 5:30 PM

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This report is devoted to application of nonlinear correlation method for allocation of connections in EEG experiments. We study the formation of the complex structure of links between different areas of brain by means of method based on the calculation of the nonlinear correlations coefficients. The nonlinear correlations analysis allows us to identify specific patterns in the EEG data set, appearing in both the cognitive processes during the perception of ambiguous objects and imaginary movements of the left and right limbs. Thus, it becomes possible to diagnose some patterns of connectivity of brain areas associated with the different cognitive tasks.


Effect of filtration of signals of brain activity on quality of recognition of brain activity patterns using artificial intelligence methods
Paper 10493-50

Author(s):  Alexander E. , Yuri Gagarin State Technical Univ. of Saratov (Russian Federation), et al.
Conference 10493: Dynamics and Fluctuations in Biomedical Photonics XV
Session PSun: Posters-Sunday
Date and Time: Sunday, January 28, 2018, 5:30 PM

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We study the problem of recognition of the different cognitive tasks by means of the registered EEG data using artificial neuronal networks. As analyzed cognitive tasks we considered both the perception of ambiguous image and imaginary movements of left and right legs. To solve the problem of recognition, we use artificial neural networks approach. We examine the influence of band-pass filtration of EEG signals on quality of recognition of brain activity patterns and show that filtering of high-frequency components of EEG leads to an increase in the recognition quality of brain activity patterns.


Analysis of bistable perception based on MEG data
Paper 10493-53

Author(s):  Vladimir A. , Saratov State Technical Univ. (Russian Federation), et al.
Conference 10493: Dynamics and Fluctuations in Biomedical Photonics XV
Session PSun: Posters-Sunday
Date and Time: Sunday, January 28, 2018, 5:30 PM

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In the present research we studied the cognitive processes, associated with the perception of ambiguous images using the multichannel MEG recordings. Using the wavelet transformation, we considered the dynamics of the neural network of brain in different frequency bands, including high (up to 100 Hz) frequency gamma-waves. Along with the time-frequency analysis of single MEG traces, the interactions between remote brain regions, associated with the perception, were also taken into consideration. As the result, the new features of bistable visual perception were observed and the effect of image ambiguity was analyzed.


Use of parallel computing for analyzing big data in EEG studies of ambiguous perception
Paper 10493-54

Author(s):  Vladimir A. , Saratov State Technical Univ. (Russian Federation), et al.
Conference 10493: Dynamics and Fluctuations in Biomedical Photonics XV
Session PSun: Posters-Sunday
Date and Time: Sunday, January 28, 2018, 5:30 PM

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Realizing the interaction between human and machine systems through the neuro-interfaces (or brain-computer interfaces) is an urgent task which requires analysis of large amount of neurophysiological EEG data. Taken into account the multichannel structure of EEG, the methods of parallel computing can be considered as the most powerful tool for the processing data in real time. In this context we demonstrate the use of parallel computing for the estimation of the spectral properties of multichannel EEG signals, associated with the visual perception. Using CUDA C library we run the algorithm of wavelet analysis on GPUs and show possibility of detection of the specific patterns in the set of EEG data in real time.


Study of the interactions in neural ensemble of the brain using wavelet analysis
Paper 10493-55

Author(s):  Vladimir A. , Saratov State Technical Univ. (Russian Federation), et al.
Conference 10493: Dynamics and Fluctuations in Biomedical Photonics XV
Session PSun: Posters-Sunday
Date and Time: Sunday, January 28, 2018, 5:30 PM

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The focal riddle for physicists and neuro-scientists consists in disclosing the way microscopic scale neural interactions pilot the formation of the different activities revealed (at a macroscopic scale) by EEG and MEG equipments. In the current paper we propose new method for detection the interactions between the remote regions of the brain, based on the wavelet analysis of EEG signals, recorded from these brain areas. With the help of the proposed approach we are able to estimate the degree of the interaction between the different parts of the brain and their participation in the generation of the different levels of consciousness.


The interaction between the meningeal lymphatics and blood-brain barrier
Paper 10495-18

Author(s):  Oxana V. , Saratov State Univ. (Russian Federation), et al.
Conference 10495: Biophotonics and Immune Responses XIII
Session 4: Novel Detection Technology
Date and Time: Monday, January 29, 2018, 4:25 PM

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Here we present new approaches based on OCT and fluorescent microscopy for imaging of lymphatic and glymphatic systems in the mouse brain. By injection of Evans Blue into the cisterna magna, we imaged the meningeal lymphatic vessels using spectral OCT and fluorescent microscopy. The unique technique of injection of gold nanorods into the brain parenchyma and OCT-imaging allowed us to observe the clearance of brain from nanorods via glymphatic pathway. These discoveries open ways to new technologies and strategies for further in vivo studies of role of cerebral lymphatic and glymphatic systems in the etiology of many neoroinflammatory and neurodegenerative diseases and their therapy.


Optoacoustic theranostics
Paper 10494-49

Author(s):  Irene Y. , The Univ. of Texas Medical Branch (United States), et al.
Conference 10494: Photons Plus Ultrasound: Imaging and Sensing 2018
Session 7: Quantitative Imaging
Date and Time: Monday, January 29, 2018, 2:45 PM

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Optoacoustic diagnostics is based on detection and analysis of optoacoustic waves induced in tissues. Recently we proposed to use optoacoustic waves for therapy (Nano-Pulse Laser Therapy-NPLT) to combine merits of low-level light and ultrasound therapies. Here we propose optoacoustic theranostics that can be used for diagnostics, therapy, and monitoring therapeutic response. We built systems for optoacoustic theranostics and tested them in rats with traumatic brain injury (TBI). Low and higher energy pulses were used for optoacoustic monitoring of cerebral brain oxygenation and for therapy, respectively. The obtained results suggest that optoacoustic theranostics may be used for TBI and other disorders.


Alzheimer's disease: evaluation using label-free fluorescence of tryptophan metabolites and the kynurenine pathway
Paper 10489-49

Author(s):  Laura A. , The City College of New York (United States), et al.
Conference 10489: Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis
Session Tues: Poster Session
Date and Time: Tuesday, January 30, 2018, 6:00 PM

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Brain tissues (Alzheimer’s disease and control) were studied using label-free fluorescence of tryptophan and its metabolites. Tryptophan (exc. 280/ em. 340 nm), N-formyl-L-kynurenine (325/434 nm), kynurenine (365/480 nm) and kynurenic acid (330/390 nm) have distinct spectral profiles. Under stress conditions, pro-inflammatory cytokines are released. These cytokines stimulate indoleamine 2,3-dioxygenase (IDO) and tryptophan 2,3-dioxygenase (TDO), which increase tryptophan metabolism through the kynurenine pathway. This can cause increased production of neurotoxic compounds. Determinations of tryptophan/metabolite ratios could potentially be used to measure IDO and TDO activity. This technique may be useful in the study of Alzheimer’s and other neurodegenerative diseases.


Feasibility of transfontanelle photoacoustic imaging: Towards neonatal functional brain imaging
Paper 10494-232

Author(s):  Mohammad R. N. , Wayne State Univ. (United States), et al.
Conference 10494: Photons Plus Ultrasound: Imaging and Sensing 2018
Session PTue: Posters-Tuesday
Date and Time: Tuesday, January 30, 2018, 6:00 PM

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To address several limitations of current clinical imaging modalities, we develop a novel transfontanelle photoacoustic imaging (TFPAI) probe, which, for the first time, should allow for non-invasive structural and functional imaging of the infant brain. TFPAI can potentially be used for bedside monitoring of neonates with various disease conditions and complications affecting brain perfusion and oxygenation, including apnea, asphyxia, as well as for detection of various types of intracranial hemorrhages (IH), such as subarachnoid (SAH), intraventricular (IVH), subependimal (SEPH), subdural (SDH), or intracerebral (ICH)


Development of a low-cost neonatal brain imaging system using photoacoustic technology: phantom study
Paper 10494-235

Author(s):  Mohammad R. N. , Wayne State Univ. (United States), et al.
Conference 10494: Photons Plus Ultrasound: Imaging and Sensing 2018
Session PTue: Posters-Tuesday
Date and Time: Tuesday, January 30, 2018, 6:00 PM

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In this study, we utilize photoacoustic computed tomography (PACT) with a hemispherical transducer array consisting of 64 single element transducers. We investigate the potential benefits of fiber bundle laser illumination for a more homogenous energy deposition than single fiber overhead illumination. Field II program was utilized to visualize the far field overlap of our transducers, which represents the volume of interest of where objects can be reconstructed. Various transducer array configurations have been simulated to show the feasibility of our low cost PACT system. We then evaluate our system's performance by imaging lead samples embedded in gelatin phantoms.


Whole-organ atlas imaged by label-free high-resolution photoacoustic microscopy assisted by a microtome
Paper 10494-93

Author(s):  Terence T. W. , Washington Univ. in St. Louis (United States), et al.
Conference 10494: Photons Plus Ultrasound: Imaging and Sensing 2018
Session 13: Microscopy
Date and Time: Wednesday, January 31, 2018, 9:15 AM

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Three-dimensional (3D) imaging of whole biological organs with microscopic resolution has remained a challenge. We demonstrated microtomy-assisted photoacoustic microscopy (mPAM) of mouse brains and other organs, which automatically acquired serial distortion-free and registration-free images with endogenous absorption contrasts. Without tissue clearing or staining, mPAM generated micrometer-resolution 3D images of paraffin- or agarose-embedded whole organs with high fidelity, achieved by label-free simultaneous sensing of DNA/RNA, hemoglobins, and lipids. With these intrinsic biological absorbers, mPAM provides histology-like imaging of cell nuclei, blood vessels, axons, and other anatomy. mPAM offers a new way to better understand complex biological organs.


Important Dates

Abstracts Due
17 July 2017

Author Notification
25 September 2017

Manuscripts Due
See Individual Conferences


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Journal of Biomedical Optics

Journal of Biomedical OpticsPublishes peer-reviewed papers that utilize modern optical technology for improved health care and biomedical research.