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Proceedings Paper

Label-free and sensitive fluorescence detection of nucleic acid, based on combination of a graphene oxid /SYBR green I dye platform and polymerase assisted signal amplification
Author(s): Xiao Zhu; Da Xing
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Paper Abstract

A new label-free isothermal fluorescence amplification detection for nucleic acid has been developed. In this paper, we first developed a novel sensitive and specific detection platform with an unmodified hairpin probe (HP) combination of the graphene oxid (GO)/ SYBR green I dye (SG), which was relied on the selective principle of adsorption and the high quenching efficiency of GO. Then for the application of this new strategy, we used Mirco RNA-21 (Mir-21) as the target to evaluate this working principle of our design. When the target was hybridizing with the HP and inducing its conformation of change, an efficient isothermal circular strand-displacement polymerization reaction was activating to assist the first signal amplification. In this format, the formed complex conformation of DNA would interact with its high affinity dye, then detached from the surface of GO after incubating with the platform of GO/intercalating dye. This reaction would accompany with obvious fluorescence recovery, and accomplish farther signal enhancement by a mass of intercalating dye inserting into the minor groove of the long duplex replication product. By taking advantage of the multiple amplification of signal, this method exerted substantial enhancement in sensitivity and could be used for rapid and selective detection of Mir-21 with attomole range. It is expected that this cost-effective GO based sensor might hold considerable potential to apply in bioanalysis studies.

Paper Details

Date Published: 11 December 2012
PDF: 8 pages
Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 855337 (11 December 2012); doi: 10.1117/12.999872
Show Author Affiliations
Xiao Zhu, South China Normal Univ. (China)
Da Xing, South China Normal Univ. (China)

Published in SPIE Proceedings Vol. 8553:
Optics in Health Care and Biomedical Optics V
Qingming Luo; Ying Gu; Xingde D. Li, Editor(s)

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