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Proceedings Paper

Thermal denaturation of protein studied by terahertz time-domain spectroscopy
Author(s): Xiuhua Fu; Xiangjun Li; Jianjun Liu; Yong Du; Zhi Hong
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Paper Abstract

In this study, the absorption spectra of native or thermal protein were measured in 0.2-1.4THz using terahertz time-domain spectroscopy (THz-TDS) system at room temperature, their absorption spectra and the refractive spectra were obtained. Experimental results indicate that protein both has strong absorption but their characteristics were not distinct in the THz region, and the absorption decreased during thermal denatured state. In order to prove protein had been denatured, we used Differential scanning calorimeter (DSC) measured their denatured temperature, from their DSC heating traces, collagen Td=101℃, Bovine serum albumin Td=97℃. While we also combined the Fourier transform infrared spectrometer (FTIR) to investigate their secondary and tertiary structure before and after denatuation, but the results did not have the distinct changes. We turned the absorption spectra and the refractive spectra to the dielectric spectra, and used the one-stage Debye model simulated the terahertz dielectric spectra of protein before and after denaturation. This research proved that the terahertz spectrum technology is feasible in testing protein that were affected by temperature or other factors,which can provide theoretical foundation in the further study about the THz spectrum of protein and peptide temperature stability.

Paper Details

Date Published: 5 December 2012
PDF: 7 pages
Proc. SPIE 8562, Infrared, Millimeter-Wave, and Terahertz Technologies II, 856218 (5 December 2012); doi: 10.1117/12.981864
Show Author Affiliations
Xiuhua Fu, China Jiliang Univ. (China)
Xiangjun Li, China Jiliang Univ. (China)
Jianjun Liu, China Jiliang Univ. (China)
Yong Du, China Jiliang Univ. (China)
Zhi Hong, China Jiliang Univ. (China)


Published in SPIE Proceedings Vol. 8562:
Infrared, Millimeter-Wave, and Terahertz Technologies II
Cunlin Zhang; Xi-Cheng Zhang; He Li; Sheng-Cai Shi, Editor(s)

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