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Proceedings Paper

Towards increased selectivity of drug delivery to cancer cells: development of a LDL-based nanodelivery system for hydrophobic photosensitizers
Author(s): Diana Buzova; Veronika Huntosova; Peter Kasak; Dana Petrovajova; Jaroslava Joniova; Lenka Dzurova; Zuzana Nadova; Franck Sureau; Pavol Miskovsky; Daniel Jancura
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Paper Abstract

Low-density lipoproteins (LDL), a natural in vivo carrier of cholesterol in the vascular system, play a key role in the delivery of hydrophobic photosensitizers (pts) to tumor cells in photodynamic therapy (PDT) of cancer. To make this delivery system even more efficient, we have constructed a nano-delivery system by coating of LDL surface by polyethylene glycol (PEG) and dextran. Fluorescence spectroscopy and confocal fluorescence imaging were used to characterize redistribution of hypericin (Hyp), a natural potent pts, loaded in LDL/PEG and LDL/dextran complexes to free LDL molecules as well as to monitor cellular uptake of Hyp by U87-MG cells. It was shown than the redistribution process of Hyp between LDL molecules is significantly suppressed by dextran coating of LDL surface. On the other hand, PEG does not significantly influence this process. The modification of LDL molecules by the polymers does not inhibit their recognition by cellular LDL receptors. U-87 MG cellular uptake of Hyp loaded in LDL/PEG and LDL/dextran complexes appears to be similar to that one observed for Hyp transported by unmodified LDL particles. It is proposed that by polymers modified LDL molecules could be used as a basis for construction of a drug transport system for targeted delivery of hydrophobic drugs to cancer cells expressing high level of LDL receptors.

Paper Details

Date Published: 10 October 2012
PDF: 10 pages
Proc. SPIE 8460, Biosensing and Nanomedicine V, 84600U (10 October 2012); doi: 10.1117/12.981831
Show Author Affiliations
Diana Buzova, Univ. of Pavol Jozef Safarik (Slovakia)
Veronika Huntosova, Univ. of Pavol Jozef Safarik (Slovakia)
Lab. Jean Perrin, CNRS, Pierre and Marie Curie Univ., Paris (France)
Peter Kasak, Polymer Institute of the Slovak Academy of the Science (Slovakia)
Dana Petrovajova, Univ. of Pavol Jozef Safarik (Slovakia)
Jaroslava Joniova, Univ. of Pavol Jozef Safarik (Slovakia)
Lab. Jean Perrin, CNRS, Pierre and Marie Curie Univ., Paris (France)
Lenka Dzurova, Univ. of Pavol Jozef Safarik (Slovakia)
Zuzana Nadova, Univ. of Pavol Jozef Safarik (Slovakia)
Franck Sureau, Lab. Jean Perrin, CNRS, Pierre and Marie Curie Univ., Paris (France)
Pavol Miskovsky, Univ. of Pavol Jozef Safarik (Slovakia)
International Laser Ctr. (Slovakia)
Daniel Jancura, Univ. of Pavol Jozef Safarik (Slovakia)


Published in SPIE Proceedings Vol. 8460:
Biosensing and Nanomedicine V
Hooman Mohseni; Massoud H. Agahi; Manijeh Razeghi, Editor(s)

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