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Proceedings Paper

Recent development in light Ultramicroscopy using aspherical optical elements
Author(s): Saiedeh Saghafi; Klaus Becker; Christian Hahn; Hans-Ulrich Dodt
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Paper Abstract

Here we present a new light sheet generator unit for Ultramicroscopy (UM) employing a combination of optical lenses with aspherical surface structure. UM allows 3D-vizualization of chemically transparent biological specimens with μm-resolution. Improving optical characteristics parameters of light sheet such as the uniformity factor of spatial intensity distribution along the line of focus, the thickness of light sheet, and chromatic aberrations are the most important criteria in this design. Since we do not use any hard edge aperture there is no truncation of the beam and laser energy is used more efficiently. Due to these improvements, a marked enhancement in presenting fine details of biological specimens such as Drosophila melanogaster, entire mouse brain, and hippocampus are achieved.

Paper Details

Date Published: 18 December 2012
PDF: 6 pages
Proc. SPIE 8550, Optical Systems Design 2012, 85500K (18 December 2012); doi: 10.1117/12.980904
Show Author Affiliations
Saiedeh Saghafi, Technische Univ. Wien (Austria)
Medical Univ. of Vienna (Austria)
Klaus Becker, Technische Univ. Wien (Austria)
Medical Univ. of Vienna (Austria)
Christian Hahn, Technische Univ. Wien (Austria)
Medical Univ. of Vienna (Austria)
Hans-Ulrich Dodt, Technische Univ. Wien (Austria)
Medical Univ. of Vienna (Austria)

Published in SPIE Proceedings Vol. 8550:
Optical Systems Design 2012
Laurent Mazuray; Daniel G. Smith; Jean-Luc M. Tissot; Tina E. Kidger; Frank Wyrowski; Stuart David; Rolf Wartmann; Jeffrey M. Raynor; Andrew P. Wood; Pablo Benítez; Andreas Erdmann; Marta C. de la Fuente, Editor(s)

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