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Proceedings Paper

The effect of an alendronate-eluting titanium system to induce osteogenic differentiation in human buccal fat cells (HBFCs)
Author(s): Sung Eun Kim; Su-Young Lee; Young-Pil Yun; Jae Yong Lee; Kyeongsoon Park; Deok-Won Lee; Hae-Ryong Song
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Paper Abstract

The purpose of this study was to develop alendronate (Aln)-eluting Ti substrates to induce osteogenic differentiation of human buccal fat cells (HBFCs). The surface of pristine Ti was modified by dopamine (DOPA) and then heparin was grafted onto the aminated Ti surfaces to achieve the Aln-eluting Ti system. Aln was subsequently immobilized on the surface of heparinized Ti (Hep-Ti). Pristine Ti and surface-modified-Ti were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and contact angle. Osteogenic differentiation of HBFCs on the surface of pristine-Ti, Hep-Ti, Aln (1 mg)/Hep-Ti, and Aln (5 mg)/Hep-Ti was demonstrated by alkaline phosphatase (ALP) activity, calcium deposition, and osteocalcin and osteopontin mRNA expression. Successful immobilization of Aln on Hep-Ti was confirmed by XPS and contact angle. Aln/Hep-Ti showed the sustained release for up to 28 days. Additionally, HBFCs cultured on Aln/Hep-Ti substrates showed significantly induced ALP activity, calcium deposition, and osteocalcin and osteopontin mRNA expression. These results suggest that Aln-eluting Ti substrates have a potential effect on osteogenic differentiation of HBFCs and will be a promising material for bone regeneration.

Paper Details

Date Published: 24 October 2012
PDF: 21 pages
Proc. SPIE 8548, Nanosystems in Engineering and Medicine, 85486B (24 October 2012); doi: 10.1117/12.979889
Show Author Affiliations
Sung Eun Kim, Korea Univ. College of Medicine (Korea, Republic of)
Su-Young Lee, St. Mary's Dental Hospital, The Catholic Univ. of Korea (Korea, Republic of)
Young-Pil Yun, Korea Univ. College of Medicine (Korea, Republic of)
Jae Yong Lee, Korea Univ. College of Medicine (Korea, Republic of)
Kyeongsoon Park, Korea Basic Science Institute (Korea, Republic of)
Deok-Won Lee, Kyung Hee Univ. Dental Hospital at Gangdong (Korea, Republic of)
Hae-Ryong Song, Korea Univ. College of Medicine (United States)

Published in SPIE Proceedings Vol. 8548:
Nanosystems in Engineering and Medicine
Sang H. Choi; Jin-Ho Choy; Uhn Lee; Vijay K. Varadan, Editor(s)

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