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Proceedings Paper

On The Water Subtraction In The Fourier Transform Infrared (FTIR) Spectra Of Proteins And Lipids
Author(s): Marie Therrien; Michel Lafleur; Michel Pezolet
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Paper Abstract

A procedure is described to subtract the buffer contribution from the infrared spectra of aqueous solutions of proteins using the 2130 cm-1 association band of water as an internal intensity standard. After correction, the spectra of several globular proteins were used to develop a method to evaluate their conformation in solution.

Paper Details

Date Published: 20 December 1985
PDF: 2 pages
Proc. SPIE 0553, Fourier and Computerized Infrared Spectroscopy, (20 December 1985); doi: 10.1117/12.970757
Show Author Affiliations
Marie Therrien, Universite Laval (Canada)
Michel Lafleur, Universite Laval (Canada)
Michel Pezolet, Universite Laval (Canada)


Published in SPIE Proceedings Vol. 0553:
Fourier and Computerized Infrared Spectroscopy
David G. Cameron; Jeannette G. Grasselli, Editor(s)

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