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Proceedings Paper

The Binding Effect Of Metal On The Secondary Structure Of Albumin
Author(s): Ly Li; Wentian Liu; Jinguang Wu; Guangxian XU
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Paper Abstract

On the basis of vibrational analysis, the deconvolution and derivative procedures of amide bands in IR spectra has stimulated the interests in the study on the secondary structure of protein [1, 2]. The inter-relationships between metal ions and binding substances such as protein are important in the living system. However, the influnce of metal ions on the secondary stucture of protein has not been studied in details by this technique. In this investigation, NaC1, KC1,ZnC12, MgC12, CaC12, AlC13, NaNO3, AI (NO3)3, PrC13,NdC13,EuC13,DyC13, TbBr3, Ce(NO3)3, andGd(NO3)3 were reacted with bovine albumin in aqueons solutions for 4-5 hours respectively. The albumin complexes were formed both in supernatants and precipitates. Their FTIR spectra were measured with BaF2 pellet for the precipitates and cast film on BaF2 windows for the supernatants, utilizing a Nicolet 7199B spectrometer.

Paper Details

Date Published: 1 December 1989
PDF: 2 pages
Proc. SPIE 1145, 7th Intl Conf on Fourier Transform Spectroscopy, (1 December 1989); doi: 10.1117/12.969528
Show Author Affiliations
Ly Li, Peking University (China)
Wentian Liu, Peking University (China)
Jinguang Wu, Peking University (China)
Guangxian XU, Peking University (China)

Published in SPIE Proceedings Vol. 1145:
7th Intl Conf on Fourier Transform Spectroscopy

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