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Proceedings Paper

A Laser Induced Fluorescence Immunoassay System For The Detection Of Salmonella And E. Coli
Author(s): R. W. Wygant; S. P. Almeida; M. D. Pierson; J. C. Hedrick; G. B. Churchill; H. P. Groger
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Paper Abstract

Total internal reflection fluorescent immunoassay techniques provide a powerful method of detecting biological antigens. A laser system has been developed to record the evanescent wave excited fluorescence emitted from the dye labeled polyclonal antibodies developed for Salmonella spp. and E. Coli 0157:H7. A cw argon laser tuned at 488 nm was used to induce the emitted fluorescence at about 520 nm. Measurement of the fluorescence was performed via an optical multichannel analyzer, on-line to a microcomputer. Antigen samples of varying concentrations were analyzed to test the operation of the system. A description of the laser detector is presented in this paper.

Paper Details

Date Published: 16 January 1988
Proc. SPIE 0952, Laser Technologies in Industry, (16 January 1988); doi: 10.1117/12.968922
Show Author Affiliations
R. W. Wygant, Virginia Polytechnic Institute (United States)
S. P. Almeida, Virginia Polytechnic Institute (United States)
M. D. Pierson, Virginia Polytechnic Institute (United States)
J. C. Hedrick, Virginia Polytechnic Institute (United States)
G. B. Churchill, American Research Corporation of Virginia (United States)
H. P. Groger, American Research Corporation of Virginia (United States)

Published in SPIE Proceedings Vol. 0952:
Laser Technologies in Industry
Silverio P. Almeida; Luis Miguel Bernardo; Oliverio D.D. Soares, Editor(s)

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