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Proceedings Paper

3-D Microscope Imaging By Optical 3-D CT
Author(s): S. Minami; O. Nakamura; S. Kawata
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Paper Abstract

The success of X-ray CT (Computed Tomography) in clinical medicine has motivated studies of nondestructive three-dimensional (3-D) observation of samples by light wave propagation through 3-D samples. The optical CT technique has been applied to 3-D measurements of refractive index, air pollutants, flow, plasma density, and birefringence. We have proposed a new method of optical 3-D CT for microscopic 3-D imaging with a conventional transmission-microscope optics and an advanced computer algorithm. By this method, inside 3-D structures of alive biological samples can be reconstructed without slicing-preparation of samples. In this paper, we describe the principle of the developed optical projeciton system with the analysis of its 3-D OTF, and the reconstruction algorithm with constraints. An experimental example of multispectral 3-D reconstruction is shown for a pollen of pine.

Paper Details

Date Published: 1 January 1987
PDF: 2 pages
Proc. SPIE 0813, Optics and the Information Age, (1 January 1987); doi: 10.1117/12.967291
Show Author Affiliations
S. Minami, Osaka University (Japan)
O. Nakamura, Osaka University (Japan)
S. Kawata, Osaka University (Japan)

Published in SPIE Proceedings Vol. 0813:
Optics and the Information Age

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