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Proceedings Paper

Molecular Dynamics Studies Using Frequency-Domain Fluorometry
Author(s): Enrico Gratton; Beniamino Barbieri
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Paper Abstract

The dynamical behavior of large molecules can be studied by measuring the fluorescence of fluorophores bound to the molecule. Time-resolved fluorescence, using frequency-domain technique, provides a unique tool for the analysis of the emission from complex systems. Time resolution of few picoseconds is attainable using state-of-the-art commercial phase and modulation fluorometers. The development of these high-resolution instruments allowed for a new understanding of the internal motions of proteins and of molecules embedded in natural and synthetic membranes.

Paper Details

Date Published: 1 January 1987
PDF: 6 pages
Proc. SPIE 0743, Fluorescence Detection, (1 January 1987); doi: 10.1117/12.966933
Show Author Affiliations
Enrico Gratton, University of Illinois at Urbana-Champaign (United States)
Beniamino Barbieri, University of Illinois at Urbana-Champaign (United States)


Published in SPIE Proceedings Vol. 0743:
Fluorescence Detection
E. Roland Menzel, Editor(s)

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