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Proceedings Paper

A 2 Ghz Frequency-Domain Fluorometer; Picosecond Resolution Of Protein Fluorescence And Anisotropy Decays
Author(s): Joseph R. Lakowicz; Gabor Laczko; Ignacy Gryczynski; Henryk Cherek; N. Joshi; M. L. Johnson
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Paper Abstract

We developed a frequency-domain fluorometer which operates from 4 to 2000 MHz. The modulated excitation is provided by the harmonic content of a laser pulse train (7.59 MHz, 5 psec) from a syncronously pumped and cavity dumped dye laser. The phase angle and modulation of the emission are measured with a microchannel plate photomultiplier. Cross-correlation detection is performed outside the PMT. The performance was verified by measurement of known time delays and examination of standard fluorophores. The detector displayed no detectable color effect, with the 300 to 600 nm difference being less than 5 psec. The precision of the measurements is adequate to detect differences of 20 psec for decay times of 500 ps.

Paper Details

Date Published: 1 January 1987
PDF: 7 pages
Proc. SPIE 0743, Fluorescence Detection, (1 January 1987); doi: 10.1117/12.966918
Show Author Affiliations
Joseph R. Lakowicz, University of Maryland (United States)
Gabor Laczko, University of Maryland (United States)
Ignacy Gryczynski, University of Maryland (United States)
Henryk Cherek, University of Maryland (United States)
N. Joshi, University of Maryland (United States)
M. L. Johnson, University of Virginia (United States)

Published in SPIE Proceedings Vol. 0743:
Fluorescence Detection
E. Roland Menzel, Editor(s)

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