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Proceedings Paper

Interplay between experimental and numerical approaches in botanical studies: development of a hybrid REC inverse method with application in mycology
Author(s): Emoke Dalma Kovacs; Radu Trambitas; Arpad Lukacs Imre; Melinda Haydee Kovacs; Dumitru Ristoiu
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Paper Abstract

A REC model (Rate Estimation from Concentrations) numerical procedure was adapted in order to estimate the production rate of selected fatty acids in some mushroom species. The production as well the distribution of these target compounds was evaluated at whole mushroom bodies as well at its different anatomical compartments. These new numerical procedure was tested with some artificial tests as well with multiple real measurements of fatty acids from all anatomical compartments of studied mushroom species. Fatty acids analyses from mushroom samples were done on gas chromatograph-mass spectrometer (GC-MS) system after a derivatization step. The amount of fatty acid methyl esters (FAMEs) from mushroom body samples ranged between 0.5 – 29.4 ng⋅kg-1 dry weights. Obtained results showed that this adapted numerical approach is optimal to estimate robust and reasonable rate and distribution profiles for relative concentration measurements of fatty acids, uncertainty being no larger than 10 %.

Paper Details

Date Published: 1 November 2012
PDF: 8 pages
Proc. SPIE 8411, Advanced Topics in Optoelectronics, Microelectronics, and Nanotechnologies VI, 84112N (1 November 2012); doi: 10.1117/12.966608
Show Author Affiliations
Emoke Dalma Kovacs, Babeş-Bolyai Univ. (Romania)
Radu Trambitas, Babeş-Bolyai Univ. (Romania)
Arpad Lukacs Imre, Babeş-Bolyai Univ. (Romania)
Melinda Haydee Kovacs, National Institute for Research and Development of Isotopic and Molecular Technologies (Romania)
Dumitru Ristoiu, Babeş-Bolyai Univ. (Romania)


Published in SPIE Proceedings Vol. 8411:
Advanced Topics in Optoelectronics, Microelectronics, and Nanotechnologies VI
Paul Schiopu; Razvan Tamas, Editor(s)

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