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Proceedings Paper

Label free detection of quadruplex formation of peroxidase-like DNAzyme by tapered fiber optic biosensor
Author(s): Z. Bagheri; Z. Chenari; Z. Karami; M. I. Zibaii; B. Ranjbar; H. Latifi
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Paper Abstract

We investigated the possibility the active DNAzyme formation of particular Guanine-rich oligonucleotide with nonadiabatic tapered optical fiber (NATOF) sensor. These kinds of oligonucleotides can form stable four-stranded structure called G-quadruplex in the presence of particular metal ions. Structural change of immobilized oligonucleotide was detected by monitoring of transmission spectrum of the sensor. The formation of active DNAzyme formed was confirmed by verifying enzyme activity. Result shows that the NATOF sensor has capability to detect intramolecular structural changes and using this sequence as biorecognition molecule for fabricating fiber optic biosensor.

Paper Details

Date Published: 17 October 2012
PDF: 4 pages
Proc. SPIE 8421, OFS2012 22nd International Conference on Optical Fiber Sensors, 84215L (17 October 2012); doi: 10.1117/12.966396
Show Author Affiliations
Z. Bagheri, Tarbiat Modares Univ. (Iran, Islamic Republic of)
Shahid Beheshti Univ. (Iran, Islamic Republic of)
Z. Chenari, Shahid Beheshti Univ. (Iran, Islamic Republic of)
Z. Karami, Tarbiat Modares Univ. (Iran, Islamic Republic of)
M. I. Zibaii, Shahid Beheshti Univ. (Iran, Islamic Republic of)
B. Ranjbar, Tarbiat Modares Univ. (Iran, Islamic Republic of)
H. Latifi, Shahid Beheshti Univ. (Iran, Islamic Republic of)


Published in SPIE Proceedings Vol. 8421:
OFS2012 22nd International Conference on Optical Fiber Sensors
Yanbiao Liao; Wei Jin; David D. Sampson; Ryozo Yamauchi; Youngjoo Chung; Kentaro Nakamura; Yunjiang Rao, Editor(s)

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