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Proceedings Paper

Application Of Laser Fluorimetry To Enzyme-Linked Immunoassay
Author(s): William D. Hinsberg; Kristin H. Milby; Steven D. Lidofsky; Richard N. Zare
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Paper Abstract

An enzyme-linked sandwich immunoassay for insulin is described. Horseradish peroxidase is employed as an enzyme label for antibody, and enzyme activity is measured via the fluorogenic substrate, p-hydroxyphenylacetic acid. The product is detected by excitation of fluorescence with the 325 nm line of a cw helium-cadmium ion laser on-line with reverse phase high performance liquid chromatography. The method requires a total incubation time of 45 minutes, and the limit of insulin detection is 1.1 μU/mℓ (6.6 pM). This assay is applicable to the analysis of human serum samples.

Paper Details

Date Published: 8 September 1981
PDF: 7 pages
Proc. SPIE 0286, Laser Spectroscopy for Sensitive Detection, (8 September 1981); doi: 10.1117/12.965825
Show Author Affiliations
William D. Hinsberg, Stanford University (United States)
Kristin H. Milby, Stanford University (United States)
Steven D. Lidofsky, Stanford University (United States)
Richard N. Zare, Stanford University (United States)

Published in SPIE Proceedings Vol. 0286:
Laser Spectroscopy for Sensitive Detection
Jerry A. Gelbwachs, Editor(s)

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