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Proceedings Paper

Optical Sectioning With The Scanning Slit Confocal Microscope: Applications In Ophthalmology And Ear Research
Author(s): Charles J. Koester; Shyam M. Khanna; Robert B. Tackaberry
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Paper Abstract

Confocal slits offer certain advantages over confocal pinholes for optical sectioning microscopy, particularly with non-laser illumination. One-dimensional scanning simplifies the instrumentation and does not generate scan lines in the image. Slit widths can be adjusted during an experiment, in order to optimize the optical section depth and image illuminance. The optical sectioning effect provided by the out-of-focus effect can be enhanced by separating the illuminating and imaging light paths through the objective lens and within the specimen except for the region of the focal plane. Cellular structures are observed in vivo in the cornea and in the cochlea.

Paper Details

Date Published: 22 December 1989
PDF: 12 pages
Proc. SPIE 1161, New Methods in Microscopy and Low Light Imaging, (22 December 1989); doi: 10.1117/12.962720
Show Author Affiliations
Charles J. Koester, Columbia University (United States)
Shyam M. Khanna, Columbia University (United States)
Robert B. Tackaberry, Tackaberry Engineering (United States)

Published in SPIE Proceedings Vol. 1161:
New Methods in Microscopy and Low Light Imaging
John E. Wampler, Editor(s)

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