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Proceedings Paper

Real-Time Confocal Imaging Of The Living Eye
Author(s): James V. Jester; H. Dwight Cavanagh; John Essepian; William J. Shields; Michael A. Lemp
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Paper Abstract

In 1986, we adapted the Tandem Scanning Reflected Light Microscope of Petran and Hadraysky to permit non-invasive, confocal imaging of the living eye in real-time. We were first to obtain stable, confocal optical sections in vivo, from human and animal eyes. Using confocal imaging systems we have now studied living, normal volunteers, rabbits, cats and primates sequentially, non-invasively, and in real-time. The continued development of real-time confocal imaging systems will unlock the door to a new field of cell biology involving for the first time the study of dynamic cellular processes in living organ systems. Towards this end we have concentrated our initial studies on three areas (1) evaluation of confocal microscope systems for real-time image acquisition, (2) studies of the living normal cornea (epithelium, stroma, endothelium) in human and other species; and (3) sequential wound-healing responses in the cornea in single animals to lamellar-keratectomy injury (cellular migration, inflammation, scarring). We believe that this instrument represents an important, new paradigm for research in cell biology and pathology and that it will fundamentally alter all experimental and clinical approaches in future years.

Paper Details

Date Published: 22 December 1989
PDF: 12 pages
Proc. SPIE 1161, New Methods in Microscopy and Low Light Imaging, (22 December 1989); doi: 10.1117/12.962719
Show Author Affiliations
James V. Jester, Georgetown University Medical Center (United States)
H. Dwight Cavanagh, Georgetown University Medical Center (United States)
John Essepian, Georgetown University Medical Center (United States)
William J. Shields, Georgetown University Medical Center (United States)
Michael A. Lemp, Georgetown University Medical Center (United States)

Published in SPIE Proceedings Vol. 1161:
New Methods in Microscopy and Low Light Imaging
John E. Wampler, Editor(s)

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