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Proceedings Paper

A Video Rate Confocal Laser Beam Scanning Light Microscope Using An Image Dissector
Author(s): Seth R. Goldstein; Thomas Hubin; Scott Rosenthal; Clayton Washburn
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Paper Abstract

A video rate confocal reflected light microscope with no moving parts has been developed. Return light from an acousto-optically raster scanned laser beam is imaged from the microscope stage onto the photocathode of an Image Dissector Tube (IDT). Confocal operation is achieved by appropriately raster scanning with the IDT x and y deflection coils so as to continuously "sample" that portion of the photocathode that is being instantaneously illuminated by the return image of the scanning laser spot. Optimum IDT scan parameters and geometric distortion correction parameters are determined under computer control within seconds and are then continuously applied to insure system alignment. The system is operational and reflected light images from a variety of objects have been obtained. The operating principle can be extended to fluorescence and transmission microscopy.

Paper Details

Date Published: 22 December 1989
PDF: 11 pages
Proc. SPIE 1161, New Methods in Microscopy and Low Light Imaging, (22 December 1989); doi: 10.1117/12.962709
Show Author Affiliations
Seth R. Goldstein, National Institutes of Health (United States)
Thomas Hubin, Chesapeake Laser Systems (United States)
Scott Rosenthal, Microsol Corp. (United States)
Clayton Washburn, Washburn Laboratory, Inc (United States)

Published in SPIE Proceedings Vol. 1161:
New Methods in Microscopy and Low Light Imaging
John E. Wampler, Editor(s)

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