Share Email Print

Proceedings Paper

Recent Advances In Light Microscopy
Author(s): Shinya Inoue
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

The combination of the light microscope with modern electronic imaging, storage, and processing devices has brought about a virtual revolution in microscopy. Dynamic structures in living cells can now be visualized with a clarity, speed, and resolution never before achieved in differential interference. contrast OTC or Nomarsky), fluorescence, polarized light, dark field, and other modes of microscopy (Fig. 1); the gliding motion, and growth and shortening, of individual molecular filaments of microtubules and factin can be followed in real time, directly on the monitor screen, (Fig. 2); and the changing concentration, and distribution. of ions and specific protein molecules can be followed, moment by moment, in physiologically active cells.1,2 In polarized light and phase contrast microscopy, optical sections as thin as 0.1 um are now attainable (Fig. 3). These images can be viewed as through-focal stacks or stereo pairs, revealing 3-dimensional architecture of biological fine structure at very high. resolution (Fig. 4). The basic principles and methods of application of video microscopy are discussed in inoue,3 and recent developments have been summarized in a conference proceedings4 (see also ref. 5,6,7).

Paper Details

Date Published: 22 December 1989
PDF: 3 pages
Proc. SPIE 1161, New Methods in Microscopy and Low Light Imaging, (22 December 1989); doi: 10.1117/12.962705
Show Author Affiliations
Shinya Inoue, Marine Biological Laboratory (United States)

Published in SPIE Proceedings Vol. 1161:
New Methods in Microscopy and Low Light Imaging
John E. Wampler, Editor(s)

© SPIE. Terms of Use
Back to Top