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Proceedings Paper

Construction Of A Novel Functional Bacterial Luciferase By Gene Fusion And Its Use As A Gene Marker In Low Light Video Image Analysis.
Author(s): A. P. Escher; D. O' Kane; J. Lee; W. H.R. Langridge; A. A. Szalay
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Paper Abstract

We have engineered a two subunit luciferase enzyme into a single functional polypeptide chain using site specific mutagenesis. We have determined, using low light video imaging, that the activity of this novel enzyme is similar to wild type luciferase when synthesized at low temperatures (15-20°C), but that it is sensitive in vivo to higher temperatures. We have used the gene encoding the monomeric bacterial luciferase as a gene marker in prokaryotic and eukaryotic organisms. Combined with low light video image analysis, it is a practical and powerful tool for quantitatively monitoring gene expression in vivo.

Paper Details

Date Published: 22 December 1989
PDF: 6 pages
Proc. SPIE 1161, New Methods in Microscopy and Low Light Imaging, (22 December 1989); doi: 10.1117/12.962702
Show Author Affiliations
A. P. Escher, University of Alberta (Canada)
D. O' Kane, University of Georgia (United States)
J. Lee, University of Georgia (United States)
W. H.R. Langridge, University of Alberta (Canada)
A. A. Szalay, University of Alberta (Canada)


Published in SPIE Proceedings Vol. 1161:
New Methods in Microscopy and Low Light Imaging
John E. Wampler, Editor(s)

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