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Proceedings Paper

Use Of Low Light Image Microscopy To Monitor Genetically Engineered Bacterial Luciferase Gene Expression In Living Cells And Gene Activation Throughout The Development Of A Transgenic Organism
Author(s): W. H.R. Langridge; A. Escher; M. Baga; D. O' Kane; J. Wampler; C. Koncz; J. Schell; A. A. Szalay
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Paper Abstract

Procaryotic and eucaryotic expression vectors which contain a marker gene for selection of transformants linked to genes encoding bacterial luciferase for detection of promoter activated gene expression in vivo were used to transform the appropriate host organisms and drug resistant colonies, cells, or calli were obtained. Bacterial luciferase expression was measured by a luminescence assay for quantitative determination of promoter activation. The cellular localization of bacteria inside the host plant cell cytoplasm was achieved in a single infected plant cell based on the light emitting ability of the genetically engineered bacteria. In addition, the bacterial luciferase marker gene fusions were used to monitor cell type, tissue, and organ specific gene expression in transgenic plants in vivo. To monitor physiological changes during ontogeny of a transformed plant, low light video microscopy, aided by real time image processing techniques developed specifically to enhance extreme low light images, was successfully applied.

Paper Details

Date Published: 22 December 1989
PDF: 14 pages
Proc. SPIE 1161, New Methods in Microscopy and Low Light Imaging, (22 December 1989); doi: 10.1117/12.962701
Show Author Affiliations
W. H.R. Langridge, University of Alberta (Canada)
A. Escher, University of Alberta (Canada)
M. Baga, University of Alberta (Canada)
D. O' Kane, University of Georgia (United States)
J. Wampler, University of Georgia (United States)
C. Koncz, Max-Planck-Institut fur Zuchtungsforschung (Germany)
J. Schell, Max-Planck-Institut fur Zuchtungsforschung (Germany)
A. A. Szalay, University of Alberta (Canada)


Published in SPIE Proceedings Vol. 1161:
New Methods in Microscopy and Low Light Imaging
John E. Wampler, Editor(s)

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