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Proceedings Paper

Simulation Tests Of Restoring Truncated Fluorescence Micrographs
Author(s): Timothy J. Holmes; Yi-Hwa Liu
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Paper Abstract

The results of a computer simulation of restoring truncated fluorescence micrographs is presented. The particular application that is considered is that of three-dimensional (3-D) optical sectioning with a conventional microscope and a photon-counting camera.1,2 Practicalities that are considered in the simulation include quantum-photon noise, the degrading effects of the missing cone in the 3-D optical transfer function (OTF), and the effects of a truncated image. The restoration algorithT used, based on maximum-likelihood estimation, has been presented previously in a nonregularized4form. Herein we present an adaptation of a regularization method developed by others for positron-emission tomography. The restored images indicate that it may be feasible to use this method for optical sectioning with a conventional microscope. We suggest that this might provide an economical alternative to optical sectioning with a confocal microscope.

Paper Details

Date Published: 22 December 1989
PDF: 8 pages
Proc. SPIE 1161, New Methods in Microscopy and Low Light Imaging, (22 December 1989); doi: 10.1117/12.962699
Show Author Affiliations
Timothy J. Holmes, Rensselaer Polytechnic Institute (United States)
Yi-Hwa Liu, Rensselaer Polytechnic Institute (United States)

Published in SPIE Proceedings Vol. 1161:
New Methods in Microscopy and Low Light Imaging
John E. Wampler, Editor(s)

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