Share Email Print
cover

Proceedings Paper

Three-Dimensional Analysis Of Molecular Distribution In Single Cells Using The Digital Imaging Microscope
Author(s): Fredric S. Fay; Walter Carrington; Larry M. Lifshitz; Kevin Fogarty
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

The development and operation of the digital imaging microscope, a system capable of analyzing the 3D molecular distribution in singe cells is described. It is a system consisting of hardware and software that: 1. obtains 2D or 3D microscope images of the faint signals from fluorescent molecules introduced into single cells; 2. reverses the distortion in such images introduced by the optics; 3. automatically or semi-automatically extracts features of interest from such images, and; 4. displays 2D, 3D images and even higher order information for interactive analysis. The strategies used to accomplish these tasks are discussed and their application to the analysis of the molecular events underlying white blood cell chemotaxis is illustrated.

Paper Details

Date Published: 22 December 1989
PDF: 12 pages
Proc. SPIE 1161, New Methods in Microscopy and Low Light Imaging, (22 December 1989); doi: 10.1117/12.962683
Show Author Affiliations
Fredric S. Fay, University of Massachusetts Medical School (United States)
Walter Carrington, University of Massachusetts Medical School (United States)
Larry M. Lifshitz, University of Massachusetts Medical School (United States)
Kevin Fogarty, University of Massachusetts Medical School (United States)


Published in SPIE Proceedings Vol. 1161:
New Methods in Microscopy and Low Light Imaging
John E. Wampler, Editor(s)

© SPIE. Terms of Use
Back to Top