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Proceedings Paper

Illumination And Detection Strategies For Confocal Microscopy
Author(s): G. J. Brakenhoff; H T. M. van der Voort
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Paper Abstract

A confocal microscope can be considered as a 3-D sampling instrument to collect data from spatial structures, especially biological ones. Optimal performance requires the adaptation of the dimensions of the sampling volume to the lateral and axial raster parameters employed during data collection. It will be shown that the shape of the sampling volume can be controlled through optical means by a combination of specific illumination and detection parameters. The use of these techniques in computer controlled instruments is discussed, especially in relation to operation in fluorescence.

Paper Details

Date Published: 28 September 1989
PDF: 5 pages
Proc. SPIE 1139, Optical Storage and Scanning Technology, (28 September 1989); doi: 10.1117/12.961783
Show Author Affiliations
G. J. Brakenhoff, University of Amsterdam (Netherlands)
H T. M. van der Voort, University of Amsterdam (Netherlands)


Published in SPIE Proceedings Vol. 1139:
Optical Storage and Scanning Technology

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